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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

NFATc4(FL)/pCAGEN (#RDB12857)

Expression clone of mouse Nfatc4

Clone info. The plasmid expressing mouse NFATc4 under the control of the CAG promoter.
Comment Expression was confirmed by the depositor with anti-NFATc4 antibody.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Nfatc4 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12857 NFATc4(FL)/pCAGEN DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The NFATc4(FL)/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12857).

Reference section:

Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12857_A4Iop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 ()
Sequence file: RDB12857_A4Ioa.seq check
>RDB12857_3_pAxCA_F1_A10_01.ab1
    1 GGCGCTGGGA AAACGAAACT GCTCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCGCC ACCATGGGGG
  121 CCGCAAGCTG CGAGGATGAG GAGCTGGAAT TTAAGCTGGT GTTCGGAGAG GAAAAGGAGC
  181 CCCCCCCGCT GGGCCCTGGG GGTCCTGGGG AAGAGCTGGA CTCAGAGGAT ACCCCTCCAT
  241 GCTGTCGTTT GGCCCTGGGG GAACCCCTTC CTTATGGTGC TGCCCCTATT GGGATTCCCC
  301 GACCCCCACC CCCTCGGCCA GGGATGCACT CACCACCGCC CCGTCCCGCC CCTTCACCTG
  361 GCACCTGGGA GAGCCAGCCA GCTAGGTCGG TGAGGCTGGG GGGCCCAGGA GGTAATGCAG
  421 GGGGTGCTGG GGGTGGTCGT GTTCTTGAGT GTCCCAGCAT CCGGATTACC TCCATCTCTC
  481 CCACGCCTGA CCCGCCGACC TCGCTAGAGG ACACATCTGA AACTTGGGGG GACGGCTCTC
  541 CTAGAGATTA CCCTCCACCA GAAGGCTTTG GGGGCTATCG AGAGGCTGGA GGTCAGGGCG
  601 GAGGCGCTTT CTTCAGCCCG AGCCCTGGCA GCAGCAGCCT GTCTTCGTGG AGCTTCTTCT
  661 CGGACGCCTC CGACGAGGCA GCCCTGTATG CAGCGTGCGA CGAGGTGGAG TCTGAACTTA
  721 ATGAAGCAGC CTCCCGTTTT GGCTTGAGCT CTCCGCTGCC CTCGCCCCGG GCTTCTCCTA
  781 GGCCATGGAC CCCGGAGGAT CCCTGGAGCC TATACGGTCC AAGCTCGGGA GGCCGAGCGC
  841 CAGAGGATAG CTGGCTACTC CTCAGCGCTC CTGGGCCCGT CCCAGCCTCC CCTCGGCCTG
  901 CTTCTCCCTG CGGCAAGCGG CGCTATTCCA GTTCCGGAAC CCCATCTTCG GCCTCCCCAG
  961 CTTTGTCCCG CCGAGGCAGC CTTGGGGAAG AAGGTCCAGA GCCACCTCCA CCACCCCCGT
 1021 TGCCTCTGGG TCCGGACCCT AGTTTCCCCT GGCCCTTTTG ACTATGTGGG TGCTCACCAC
 1081 CGAGAGCCAT CCCTCAGAAA CTCGGAGGAC TTCTAGCGAG CAGCCATGAT CCTGCTTCGT
1141 CTGAGGAGCC CTCCCCTCGG TGCCATGGGG AGC
//
Primer: pCA_bgter ()
Sequence file: RDB12857_A4Iob.seq check
>RDB12857_3_pCA_bgter_B10_04.ab1
    1 AGCCCGGAGG TCCCATAATT TTTGGCAGAG GGAAAAAGAT CTCAGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGCGG
  121 CCGCTCAGGC AGGAGGCTCT TCTCCAGGAC GTGCAGGGAA GCCACTCAGG TCTCGGCCAA
  181 TGATCTCACT CACTTCCTCC AGGGTGATAC CCTGGATAGG TACACTGTCT CTGAAGCCGC
  241 TGCTGTAGCC ACCCCTGGCT TTCTCCTGCT CCGAAGCCCC CTCCCCAGGG GTATAGCCTG
  301 GCCCCACCTC ATTGTAGCCC TCAGGAGGTA AGGGGTGGAT GGCACTCTGG GGATGGAAGG
  361 GGTCTTCGAG TGGTGGGGAG GAAGGCAGTG GCGGCCTAAA GGGGGCTGGA GGGGAGAATG
  421 GAAGCCCCAG GGCAAAAGAG GAGCCCTGTC CTCCATATTG ATCACCAGGA AAGGGGCGCG
  481 GAAGGAAAGA GACTGAAGGT AGGCGGGCAC AACCTGTGGT ACCCCCAGTC TCAGGGAACA
  541 TCCGCAGGCC AGATCTATAG GACGGTGGGG GCCCCGTCTG GGGGTACAGA GCGGGTGTGC
  601 TATAACCAAA GCCTTCTGAC AGATAAGGAG TCTCATAAGC AGGGTCTTCA TGGGGATAGG
  661 AGGGGTAAGG GGGCCTGGGC GGTGAGAAGT CCACGTCAGG GCCAAAGGGG GGACCCGATG
  721 TGGAAGGGAA GCCCCGGAGA GATGAGTCTG GTAGGGGCTC CTCCTTGAAG ACCACAGGTA
  781 GGAACTTGAA ACTTTGGGTA GGACTGCGCT TCCTCCGTCC ATTGGAGACG TAAAAGTAGA
  841 CCTGGACTGG TCGGGACACC CGCTTGTTGC TGTACTCGGG GATGGTGAGA GTCAATGTCA
  901 CCTCACTGCT CTGCAGTCGG TTCACCGCTG CCTCTTCCTC CCACTGCAGT TTTCCATCAG
  961 GACCCCTCTC AATGAACACC ACTTTAGAGT CTGGCAGGAA GTTGGAACCA GTCAACACTA
 1021 GCTCCTCCTC TCCTCTCACA GAGCAGGCAC TGGGGGCTGT AGGGTCTCCA CCTGGGGCAG
 1081 CTCCTTGGGT CGAGCGCTGG GGAGCACTCG ATGGTCACTT GATGCCTGCT GCACAGAACA
1141 CGAACCCATC CCGCCCGCCC CTGGAGGCAC ACGGGTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]