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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Dscr1 sh#2/pBS-U6

Plasmid expressing shRNA against Dscr1

Catalog number RDB12856
Resource name Dscr1 sh#2/pBS-U6
Clone info. The plasmid expressing shRNA against Dscr1.
Vector backbone pBS-U6 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Dscr1
Depositor|Developer Kurabayashi, Nobuhiro |
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External Database
Mouse Dscr1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

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          RDB12856 Dscr1 sh#2/pBS-U6 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Dscr1 sh#2/pBS-U6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12856).

          Reference section:

          Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12856_A4Imp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp ()
          Sequence file: RDB12856_A4Ima.seq check
          >RDB12856_3_T7_20bp_E03_15.ab1
              1 TGCTGCACTG ACTAGTGGAT CCGACGCCGC CATCTCTAGG CCCGCGCCGG CCCCCTCGCA
             61 CAGACTTGTG GGAGAAGCTC GGCTACTCCC CTGCCCCGGT TAATTTGCAT ATAATATTTC
            121 CTAGTAACTA TAGAGGCTTA ATGTGCGATA AAAGACAGAT AATCTGTTCT TTTTAATACT
            181 AGCTACATTT TACATGATAG GCTTGGATTT CTATAAGAGA TACAAATACT AAATTATTAT
            241 TTTAAAAAAC AGCACAAAAG GAAACTCACC CTAACTGTAA AGTAATTGTG TGTTTTGAGA
            301 CTATAAATAT CCCTTGGAGA AAAGCCTTGT TTGGGCCAGG AGAGAAGTAT GAAAAGCTTT
            361 TTATACTTCT CTCATGGGCC AGGTAAGGTT TTTCTCCAAG GGATAATTAT AGAATATAAA
          421 C
          //
          Primer: Reverse2 ()
          Sequence file: RDB12856_A4Imb.seq check
          >12856_12856_A4Im_3_Reverse2_H06_08_ABI08.ab1
              1 GTTCCCAGGC ATTTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA
             61 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCAA
          121 AAAGGGCCAG GAGATAAAAA TAATATA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl