Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Dscr1-pCAGEN (#RDB12852)

Expression clone of mouse DSCR1.1L

Alternative name Dscr1.1L/pCAGEN
Clone info. The plasmid expressing mouse DSCR1.1L under the control of the CAG promoter.
Comment Expression was confirmed by the depositor with anti-DSCR1 antibody.
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Dscr1 cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12852 Dscr1-pCAGEN DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Dscr1-pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12852).

Reference section:

Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12852_A4Imp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB12852_A4Ima.seq check
>RDB12852_2_pAxCA_F1_C03_09.ab1
    1 CGAGCCCTGT CAACATGTTA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA CCATGGAGGA CGGCGTGGCC
  121 GGGCCGCGGC TCGGAGAGGT GGCGGAGGCG GTCGAGGCAC GGGCGCCCCG TAGGGTGACT
  181 CTGCGGCCCT TCGCGCCCTT CTCTGCGGCT GCCGAGGGGG ACGGGGGCGG CGGCGGCGAC
  241 TGGAGCTTCA TCGACTGCGA GATGGAGGAG GTGGATCTGC AGGACCTGCC GAGCGCCACC
  301 ATCGCCTGCC ACCTGGACCC GCGCGTGTTC GTGGACGGCC TGTGCCGGGC CAAATTTGAA
  361 TCCCTCTTCA GAACATATGA CAAGGACACC ACCTTCCAGT ATTTTAAGAG CTTCAAACGT
  421 GTCCGGATAA ACTTCAGCAA CCCCTTATCT GCAGCCGATG CCAGGCTGCG GCTGCACAAG
  481 ACCGAGTTCC TGGGGAAGGA AATGAAGTTG TATTTTGCTC AGACTTTACA CATAGGAAGT
  541 TCACACCTGG CTCCGCCCAA TCCCGACAAA CAGTTCCTCA TCTCCCCTCC GGCCTCTCCT
  601 CCCGTTGGCT GGAAACAAGT AGAAGATGCC ACCCCCGTCA TAAATTACGA TCTTTTATAT
  661 GCCATCTCCA AGCTGGGGCC AGGAGAGAAG TATGAACTGC ATGCAGCGAC AGACACCACT
  721 CCCAGTGTGG TGGTCCACGT GTGTGAGAGT GACCAAGAGA ATGAGGAGGA AGAGGAAGAG
  781 ATGGAGAGAA TGAAGAGACC CAAGCCCAAA ATCATCCAGA CACGGAGACC GGAGTACACA
  841 CCCATCCACC TCAGCTGAGC GGCCGCACTC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG
  901 TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA
  961 AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTTCTGGCTA ATAAGGAAAT
 1021 TTATTTTCAT TGCATAGTGT GTTGGAAATT TTTTGTGTCT CTCACTCGGA GACATATGGG
 1081 AGGCAAATCA ATTAAACATC AGATGAGTAT TGGGTTAGAG TGCCACTATG GCCATATGCT
1141 GGCTTGCCAT GAACCAAGGT TGGCCTAATT AAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]