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Dyrk1a/pCAGEN (#RDB12851)

Expression clone of mouse DYRK1A

Clone info. The plasmid expressing mouse DYRK1A under the control of the CAG promoter.
Comment Expression was confirmed by the depositor with anti-DYRK1A antibody. Known differences (at least): compared with NM_007890.2, C to G substitution at 2633nt (Ala to Gly 743aa).
Vector backbone pCAGEN (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Dyrk1a cDNA
Depositor|Developer Kurabayashi, Nobuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013))
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013))
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12851 Dyrk1a/pCAGEN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Dyrk1a/pCAGEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12851).

Reference section:

Kurabayashi, N., Sanada, K., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12851_A6Igp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1_ ()
Sequence file: RDB12851_A6Igc.seq check
>12851_12851_A6Ig_pAxCA_F1_D04_04_ABI08.ab1
    1 GGTTCCCCTG CTACATGGTT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCG CCACCATGCA TACAGGAGGA
  121 GAGACTTCAG CATGCAAACC TTCATCTGTC CGGCTTGCAC CGTCGTTCTC ATTCCATGCT
  181 GCTGGCCTTC AGATGGCTGC ACAGATGCCC CACTCACACC AGTACAGTGA CCGTCGCCAG
  241 CCGAGCATAA GTGACCAGCA GGTGTCTGCC TTACCATATT CTGACCAGAT TCAGCAACCT
  301 CTAACTAACC AGGTGATGCC TGACATTGTC ATGTTACAGA GGCGGATGCC CCAAACCTTC
  361 CGTGATCCAG CAACTGCTCC TCTGAGAAAA CTCTCTGTGG ACTTGATCAA AACATACAAG
  421 CATATTAATG AGGTTTACTA TGCAAAAAAG AAGCGAAGAC ACCAACAGGG CCAGGGGGAC
  481 GATTCCAGTC ATAAGAAGGA GCGGAAGGTT TACAATGATG GTTACGATGA TGATAACTAT
  541 GATTATATTG TAAAAAACGG GGAAAAGTGG ATGGATCGGT ATGAAATCGA CTCCTTAATA
  601 GGCAAAGGTT CATTTGGACA GGTTGTGAAA GCTTATGACA GAGTGGAGCA AGAATGGGTC
  661 GCCATTAAAA TCATCAAGAA CAAGAAAGCG TTTCTGAATC AAGCCCAGAT AGAAGTGCGG
  721 CTGCTTGAGC TCATGAACAA ACACGACACT GAAATGAAGT ACTACATAGT GCATTTGAAA
  781 CGCCACTTTA TGTTTCGAAA CCATCTCTGT TTAGTGTTTG AAATGCTGTC CTATAATCTC
  841 TATGATTTGT TGAGAAACAC CAACTTCCGA GGGGTCTCTT TGAACCTAAC ACGAAAGTTT
  901 GCGCAACAGA TGTGCACAGC ATTGCTTTTT CTTGCGACTC CAGAACTTAG TATCATTCAC
  961 TGTGACTTAA AGCCTGAGAA CATCCTTCTG TGTAACCCCA AACGGAGTGC AATCAAGATT
 1021 GTTGATTTTG GGCAGCTCTT GTCAGTTGGG GCAGAGGATA TACCAGTATT ATTCAGAGTC
 1081 GCTTTTATCG GTCTCCAGAG GTGCTACTGG GAAATGCCTT ATGACCTTGC TATCGACATG
 1141 TGTCCCTTGG ATGTTATTCT GTTGAATGCA CACTGTAGAG CTCTGGTCAA TGTGCATTGA
1201 AGTTCGATTC AGATGAAATT AAAAATATGT TGCAAAGGT
//
Primer: pCA_bgter ()
Sequence file: RDB12851_A6Igd.seq check
>12851_12851_A6Ig_pCA_bgter_C05_03_ABI08.ab1
    1 CGCCCTGAAG TTCCATAATT TTTGGCAGAG GGAAAAAGAT CTCAGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGCGG
  121 CCGCTCAATG TAGTCACGAG CTAGCTACAG GACTCTGTTG CACACAAACT CCTGTCATGG
  181 GAGACTCTTC TCTATCACCC CCTTGTCTCA TCGCCAGATG TCCTTCAGTC ATGTAATGTG
  241 CAGGACCTGT ATTAGCAGAA AATTGGTATG TTGGATGTCC AGCTATGCCA GTCTCTTGGC
  301 GGGGATTGGA GTAGACGGTC AAATTAACGT CCATAGCTCC ATTCTGTCCA AAGTCCAAGG
  361 TGTTAGCAGC CACTGGGCGG TTCTGATAGG CCTGATTGCC TTGATTACCA GTAGAGGAGG
  421 AAGATGTCGA GGAAGAAGTT GTTGAGGAAG ACAGGGATGT CATGGAGTGG TGACTGTGGC
  481 CAACCTCCAT AGAATCCTGG GTAGAGGAGC TATTTGTTGG AGAATTGTAG ACCCTTGGCC
  541 TGGTCCGGTT ACCCAAGGCT TGCTGTCCAT GGTGGTGGTG GTGGTGATGG TGGTGGCATG
601 AAATACAGAC CCTGTCTTTT CTTCG
//
Primer: Dyrk1a_F1 ()
Sequence file: RDB12851_A6Ige.seq check
>12851_12851_A6Ig_Dyrk1a_F1_F04_06_ABI08.ab1
    1 TTCCAGAGTT AATTTGAGGT TTTTCTATGG CAAAAAAGAA GCGAAGACAC CAACAGGGCC
   61 AGGGGGACGA TTCCAGTCAT AAGGAAGGAG CGGAAGGTTT ACAATGATGG TTACGATGAT
  121 GATAACTATG ATTATATTGT AAAAAACGGG GAAAAGTGGA TGGATCGGTA TGAAATCGAC
  181 TCCTTAATAG GCAAAGGTTC ATTTGGACAG GTTGTGAAAG CTTATGACAG AGTGGAGCAA
  241 GAATGGGTCG CCATTAAAAT CATCAAGAAC AAGAAAGCGT TTCTGAATCA AGCCCAGATA
  301 GAAGTGCGGC TGCTTGAGCT CATGAACAAA CACGACACTG AAATGAAGTA CTACATAGTG
  361 CATTTGAAAC GCCACTTTAT GTTTCGAAAC CATCTCTGTT TAGTGTTTGA AATGCTGTCC
  421 TATAATCTCT ATGATTTGTT GAGAAACACC AACTTCCGAG GGGTCTCTTT GAACCTAACA
  481 CGAAAGTTTG CGCAACAGAT GTGCACAGCA TTGCTTTTTC TTGCGACTCC AGAACTTAGT
  541 ATCATTCACT GTGACTTAAA GCCTGAGAAC ATCCTTCTGT GTAACCCCAA ACGGAGTGCA
  601 ATCAAGATTG TTGATTTTGG CAGCTCTTGT CAGTTGGGGC AGAGGATATA CCAGTATATT
  661 CAGAGTCGCT TTTATCGGTC TCCAGAGGTG CTACTGGGAA TGCCTTATGA CCTTGCTATC
  721 GACATGTGGT CCCTTGGGAT GTATCTTGGT TGAAATGCAC ACTGGAGAGC CTCTGTTCAG
  781 TGGTGCCAAT GAGGTCGATC AGATGAATAA AAATAGTGGA AGTCTTGGGC ATCCCCACCT
  841 GCTCATATTC TTGACCAAGC ACCGAAAGCA AGAAAGTTCT TTGAGAAGTT GCCCGATGGC
  901 ACTTGGAGCT TAAAGAAGAC CAAAAGATGG AAAACGGGAG TACAACCACC AGGAAACCCG
  961 TAAACTTCAT AATATTCTTT GAAATAGAAA CAGAGGACCT TGGCGGGCGG CGTGCTGGGG
 1021 ATCGGGTCAT ACTGTAGCTG ACTACTTGAA GTTCAAAGAC CTCATTTTAA GGATGCTGAT
 1081 TTATGACCCC CAAACTCGGA TTCAACCTTA TTATGGCCCT GCAGCCACAG TTTTTTCACA
 1141 GGAAGACAGC TGGATGAAGT ACCCAACTCA AGTTACAAGG TGTGTCTACC AAGCCCCTGG
 1201 CCATTGGAAG CATCCCTCGA GTCCTCAGGC ACCACCTCCC AGCACCCTTC CCTCAGACTC
1261 AAGGGGTGGA TCTCCCTGTG GCAAACCGCA G
//
Primer: Dyrk1a_F2 ()
Sequence file: RDB12851_A6Igf.seq check
>12851_12851_A6Ig_Dyrk1a_F2_B05_02_ABI08.ab1
    1 ATGGGAAATT GGGGAAGCTC TTGTCAGGTT GGGGCAGAGG ATATACCAGT ATATTCAGAG
   61 TCGCTTTTAT CGGTCTCCAG AGGTGCTACT GGGAATGCCT TATGACCTTG CTATCGACAT
  121 GTGGTCCCTT GGATGTATCT TGGTTGAAAT GCACACTGGA GAGCCTCTGT TCAGTGGTGC
  181 CAATGAGGTC GATCAGATGA ATAAAATAGT GGAAGTCTTG GGCATCCCAC CTGCTCATAT
  241 TCTTGACCAA GCACCGAAAG CAAGAAAGTT CTTTGAGAAG TTGCCCGATG GCACTTGGAG
  301 CTTAAAGAAG ACCAAAGATG GAAAACGGGA GTACAAACCA CCAGGAACCC GTAAACTTCA
  361 TAATATTCTT GGAGTAGAAA CAGGAGGACC TGGCGGGCGG CGTGCTGGGG AATCGGGTCA
  421 TACTGTAGCT GACTACTTGA AGTTCAAAGA CCTCATTTTA AGGATGCTTG ATTATGACCC
  481 CAAAACTCGG ATTCAACCTT ATTATGCCCT GCAGCACAGT TTTTTCAAGA AAACAGCTGA
  541 TGAAGGTACC AACACAAGTA ACAGTGTGTC TACCAGCCCT GCGATGGAGC AGTCTCAGTC
  601 TTCAGGCACC ACCTCCAGCA CCTCCTCCAG CTCAGGTGGA TCCTCGGGAA CGAGTAACAG
  661 TGGGAGAGCC AGGTCGGATC CGACGCACCA GCATCGACAC AGCGGTGGAC ACTTCGCTGC
  721 TGCTGTCCAG GCCATGGACT GTGAGACACA CAGTCCCCAG GTGCGCCAGC AGTTTCCGGC
  781 TCCTCTGGGA TGGTCAGGCA CTGAAGCTCC TACACAAGTC ACTGTTGAAA CTCATCCTGT
  841 TCAAGAGACA ACCTTTCATG TAGCCCCCCA GCAGAACGCA TTGCATCATC ACCATGGAAA
  901 CAGTTCCCAT CACCACCACC ACCACCATCA CCACCACCAC CACCATGGGA CAGCAAGCCT
  961 TGGGTAACCG GGACCAGGCC AAGGGTCTAC AATTCTCCAA CAAATAGCTC CTCTACCCAG
 1021 GATTCTATGG AGGTTGGCCA CAGTCACACT CCATGACATC CTGTCTTCCT CAACAACTTC
 1081 TTCTCGACAT CTTCTCCTCT ACTGGTAATC AAGGCAATCA AGCTATCAGA AGCGGCCCAG
 1141 TTGCTGCTTA CACTGGGACT TTGGGACAGA ATGAAGCCTA TGGACGTTTA ATTGGAACCG
1201 GTCTATACTT CCCATATCTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kurabayashi, N., Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells. Genes Dev. 27 (24): 2708-2721 (2013). PMID 24352425. [link to RRC of NBRP]