Please review the QC test results indicated by icon below as well as clone information before placing your order.

Histac pcDNA3.1

For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please use Schedule A (GFP-A) additionally. Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009)。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
付加的提供条件	利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 宮脇敦史博士による提供承諾書 [Word]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12840_A4Ihp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB12840_A4Iha.seq
>RDB12840_3_CMV_Forward_B11_05.ab1     1 TCTTACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCATGGTGAG   181 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT   241 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT   301 GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC   361 CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA   421 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA   481 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG   541 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA   601 GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA   661 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA   721 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG   781 CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA   841 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG AATTCATGTC   901 TCTGCCAAGT CGACAAACAG CTATTGTTAA CCCCCCTCCA CCAGAGTACA TAAACACTAA   961 GAAGAGTGGG CGGGTTGACG AATCAGCTGC AGTTCCTACA GAGGGTTGTG CTGAAGGCCC  1021 TGTGGAAGCA CGGCTTCTCT TGGCCTTTCC ACAGCCCGGT GGACGCCGTG AACTAAGCTG  1081 CCCTGACTAT TACACCATCA TAAAAAACCC CCAATGGATT GAATACATAG AGCGTTTGAA 1141 AAATAAGTAT TTTATGAAGA AAAGGCCTTC CGGAAATG //
Primer: attB2-R1 Sequence file: RDB12840_A4Ihb.seq
>12840_12840_A9ErF_3_attB2-R1_C01_03_ABI08.ab1     1 GCCGGTCAAG AGGTGGCGGG GCCCTGGGCG CTTGAGCGCG TACACCACGT CCATAGCCGT    61 GACCGTCTTG CGCTTGGCGT GCTCGGTGTA GGTGACGGCG TCGCGGATCA CATTCTCAAG   121 GAAAACCTTG AGGACACCGC GAGTCTCCTC GTAGATGAGA CCCGAGATGC GCTTCACGCC   181 GCCACGCCGA GCCAACCGCC GGATAGCGGG CTTGGTAATG CCCTGGATGT TGTCGCGGAG   241 AACCTTGCGG TGTCGCTTGG CGCCACCTTT CCCCAGACCC TTGCCGCCTT TTCCTCGTCC   301 TGACATGGAT CCAGATCCTC CACCACCTGA ACCACCACCT CCAGAACCTC CTCCACCAGA   361 TCCACCTCCT CCGTCGACTG GAACTTGGGA CAGAACTTGC AGCTGCTGAT GAACAGCATT   421 AAGCTGCTCC TGAAGCTTGG CAAGATGCTG CACTCGTTCA TCCTCAGAGT CTTCAGAAGA   481 GGCGTCCCCC GAGGAGGCCT CGCTGCTGCT TTCTCTGCTG AGGGCTTGTG CACTGTTTGT   541 TGTAAGGTGG CATGCATGCA TACTCTCAAT AGGTTCATCG GGAATCTTGG CAAAATGTAG   601 TTCAAAAACA TCCTGAAGGG TCCGAGCCAT CGCCACAACT TCATGATCGG GAGGATTATA   661 CTTGTAACAG TTCATGAACA TCAGTCTGAC ATCTGCAGCA AATTCATATG CATCCTTATA   721 TTCTTGATTG TCCATTTTCC CCTTGATCGT TCCGAGGATC CATGGGATTT TTGACAAACG   781 TCATAGTAAG TTGTGGAGTC CCAAAAGCAT CAGCATCCAC AGGATTATAG AAGGGGGCCA   841 TGCATAACGG CAAAATGTTT TCTTGGCAAA GCATTTCTTT TAAAAATCTC GCTTGCCAGT   901 GCTTTTAATT TGCTCCCGTT ACTTTGAACT GTTTTTAAAA ACTTTGGTGT TGGTTGCTGA   961 AAAGGTCTGG GCAAAAACGT TTCTTTTACT GGAATTTTTC TTTTTTACTG GGCATTGTTC  1021 CACTTGGCTT TTTGTTTTCC TCTAAGTTGT CGGAAAGGAG AATTTCCCCT TACCTTAGAC  1081 TTTAGACCTA ATAGGACAAT GTGTGAAGAG AGATTTAGTT GGTCGCTCTG TGCTTCTGAC 1141 CGTACCGTCA CCCCACCCCC TCTTTCATGT TAAGATGT //
Primer: BGH_rev Sequence file: RDB12840_A4Ihc.seq
>RDB12840_3_BGH_rev_C11_08.ab1     1 CAGGTCCGGG CCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC    61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT   121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT   181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG   241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG   301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT   361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT   421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT   481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC   541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA   601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT   661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA   721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC   781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC   841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC   901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC   961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGGT AATGCCCTGG ATGTTGTCGC  1021 GGAGAACCCT TGCGGTGTCG CTTGGCGCCA CCTTTCCCCA GACCCTTGCC GCCTTTTCTC  1081 GTCCTGACAT GATCAGATCT CCACACCTGA ACCACAACTT CAGACTTCTT CAACCAGATC 1141 CACCTCCTTC CGGATCGACC TGAACTTTGG C //
Primer: SV40pro_F_V2 Sequence file: RDB12840_A4Ihd.seq
>RDB12840_3_SV40pro_F_V2_H05_23.ab1     1 ACAAGAGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG TATATCCATT    61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG   121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG   181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT   241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA   301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG   361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT   421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT   481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG   541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA   601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC   661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC   721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT   781 ATTGCTGAAG AGCTTGCGGC GAATGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC   841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGACTC   901 TGGGGTTCGA AATGACCGAC AGCGACGCCC ACTGCCATCA CGAGATTTCG ATCACGCGCT   961 TCTATGAAGT GGGCCTCGGA TCGTTTCGGG ACGCGCTGGA TGATCTCAGC GCGGGGATCT  1021 CATGCTGGAG TCTCGCCACC CAACCTGGTT ATGCAGCTAA TGGTACCAAT AGCATGCATC 1081 ATTACCAATA GGCATTTTCC ACTTGCATCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.07.10

GNP_filter3_RDBDEP_html_210618.pl