Resource data sheet
Prev.

Histac pcDNA3.1

For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.

Catalog number RDB12840
Resource name Histac pcDNA3.1
Clone info. For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.
Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.5kb
Gene/insert name Mouse testis-specific bromodomain cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor Sasaki, Kazuki |
 
Other clones in our bank mouse Brdt (NCBI Gene 114642) | mouse Hist4h4 (NCBI Gene 320332) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12840 Histac pcDNA3.1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12840z.seq from Depositor
Electronic file PDF (map, protocol and/or remarks) RDB12840.pdf from Depositor

Original reference

original Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Epigenetics clone (English text)
Featured content Histac for visualization of acetylation of the Histon (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12840_A4Ihp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12840_A4Iha.seq
>RDB12840_3_CMV_Forward_B11_05.ab1
    1 TCTTACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCATGGTGAG
  181 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  241 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  301 GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  361 CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA
  421 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  481 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  541 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  601 GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA
  661 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA
  721 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
  781 CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  841 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG AATTCATGTC
  901 TCTGCCAAGT CGACAAACAG CTATTGTTAA CCCCCCTCCA CCAGAGTACA TAAACACTAA
  961 GAAGAGTGGG CGGGTTGACG AATCAGCTGC AGTTCCTACA GAGGGTTGTG CTGAAGGCCC
 1021 TGTGGAAGCA CGGCTTCTCT TGGCCTTTCC ACAGCCCGGT GGACGCCGTG AACTAAGCTG
 1081 CCCTGACTAT TACACCATCA TAAAAAACCC CCAATGGATT GAATACATAG AGCGTTTGAA
1141 AAATAAGTAT TTTATGAAGA AAAGGCCTTC CGGAAATG
//
Primer: attB2-R1 RDB12840_A4Ihb.seq
>12840_12840_A9ErF_3_attB2-R1_C01_03_ABI08.ab1
    1 GCCGGTCAAG AGGTGGCGGG GCCCTGGGCG CTTGAGCGCG TACACCACGT CCATAGCCGT
   61 GACCGTCTTG CGCTTGGCGT GCTCGGTGTA GGTGACGGCG TCGCGGATCA CATTCTCAAG
  121 GAAAACCTTG AGGACACCGC GAGTCTCCTC GTAGATGAGA CCCGAGATGC GCTTCACGCC
  181 GCCACGCCGA GCCAACCGCC GGATAGCGGG CTTGGTAATG CCCTGGATGT TGTCGCGGAG
  241 AACCTTGCGG TGTCGCTTGG CGCCACCTTT CCCCAGACCC TTGCCGCCTT TTCCTCGTCC
  301 TGACATGGAT CCAGATCCTC CACCACCTGA ACCACCACCT CCAGAACCTC CTCCACCAGA
  361 TCCACCTCCT CCGTCGACTG GAACTTGGGA CAGAACTTGC AGCTGCTGAT GAACAGCATT
  421 AAGCTGCTCC TGAAGCTTGG CAAGATGCTG CACTCGTTCA TCCTCAGAGT CTTCAGAAGA
  481 GGCGTCCCCC GAGGAGGCCT CGCTGCTGCT TTCTCTGCTG AGGGCTTGTG CACTGTTTGT
  541 TGTAAGGTGG CATGCATGCA TACTCTCAAT AGGTTCATCG GGAATCTTGG CAAAATGTAG
  601 TTCAAAAACA TCCTGAAGGG TCCGAGCCAT CGCCACAACT TCATGATCGG GAGGATTATA
  661 CTTGTAACAG TTCATGAACA TCAGTCTGAC ATCTGCAGCA AATTCATATG CATCCTTATA
  721 TTCTTGATTG TCCATTTTCC CCTTGATCGT TCCGAGGATC CATGGGATTT TTGACAAACG
  781 TCATAGTAAG TTGTGGAGTC CCAAAAGCAT CAGCATCCAC AGGATTATAG AAGGGGGCCA
  841 TGCATAACGG CAAAATGTTT TCTTGGCAAA GCATTTCTTT TAAAAATCTC GCTTGCCAGT
  901 GCTTTTAATT TGCTCCCGTT ACTTTGAACT GTTTTTAAAA ACTTTGGTGT TGGTTGCTGA
  961 AAAGGTCTGG GCAAAAACGT TTCTTTTACT GGAATTTTTC TTTTTTACTG GGCATTGTTC
 1021 CACTTGGCTT TTTGTTTTCC TCTAAGTTGT CGGAAAGGAG AATTTCCCCT TACCTTAGAC
 1081 TTTAGACCTA ATAGGACAAT GTGTGAAGAG AGATTTAGTT GGTCGCTCTG TGCTTCTGAC
1141 CGTACCGTCA CCCCACCCCC TCTTTCATGT TAAGATGT
//
Primer: BGH_rev RDB12840_A4Ihc.seq
>RDB12840_3_BGH_rev_C11_08.ab1
    1 CAGGTCCGGG CCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC
   61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
  121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
  181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG
  241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
  301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
  361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
  421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
  481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC
  541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
  601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
  661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
  721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC
  781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC
  841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
  901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
  961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGGT AATGCCCTGG ATGTTGTCGC
 1021 GGAGAACCCT TGCGGTGTCG CTTGGCGCCA CCTTTCCCCA GACCCTTGCC GCCTTTTCTC
 1081 GTCCTGACAT GATCAGATCT CCACACCTGA ACCACAACTT CAGACTTCTT CAACCAGATC
1141 CACCTCCTTC CGGATCGACC TGAACTTTGG C
//
Primer: SV40pro_F_V2 RDB12840_A4Ihd.seq
>RDB12840_3_SV40pro_F_V2_H05_23.ab1
    1 ACAAGAGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG TATATCCATT
   61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
  661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
  721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
  781 ATTGCTGAAG AGCTTGCGGC GAATGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
  841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGACTC
  901 TGGGGTTCGA AATGACCGAC AGCGACGCCC ACTGCCATCA CGAGATTTCG ATCACGCGCT
  961 TCTATGAAGT GGGCCTCGGA TCGTTTCGGG ACGCGCTGGA TGATCTCAGC GCGGGGATCT
 1021 CATGCTGGAG TCTCGCCACC CAACCTGGTT ATGCAGCTAA TGGTACCAAT AGCATGCATC
1081 ATTACCAATA GGCATTTTCC ACTTGCATCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290.
reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.
user report Dancy, B.M., Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition. Chembiochem. 13 (14): 2113-2121 (2012). PMID 22961914.

2019.05.30

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