Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Histac pcDNA3.1

For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.

Catalog number RDB12840
Resource name Histac pcDNA3.1
Clone info. For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.
Comment Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.5kb
Gene/insert name Mouse testis-specific bromodomain cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Sasaki, Kazuki |
Other clones in our bank

External Database
Mouse testis-specific bromodomain Mouse Histone H4 Aequorea victoria GFP

        Reference sequence
          
         
        Sequence RDB12840z.seq
        Remarks, protocol and/or map (pdf) RDB12840.pdf

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
        Additional terms and conditions:
        RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009)。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
        付加的提供条件:
        利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
        宮脇敦史博士による提供承諾書 [Word]
        備考
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB12840 Histac pcDNA3.1 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Epigenetics clone (English text)
        Featured content Histac for visualization of acetylation of the Histon (English text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB12840_A4Ihp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Sequence file: RDB12840_A4Iha.seq check
        >RDB12840_3_CMV_Forward_B11_05.ab1
            1 TCTTACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
           61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
          121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCATGGTGAG
          181 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
          241 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
          301 GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
          361 CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA
          421 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
          481 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
          541 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
          601 GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA
          661 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA
          721 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
          781 CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
          841 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG AATTCATGTC
          901 TCTGCCAAGT CGACAAACAG CTATTGTTAA CCCCCCTCCA CCAGAGTACA TAAACACTAA
          961 GAAGAGTGGG CGGGTTGACG AATCAGCTGC AGTTCCTACA GAGGGTTGTG CTGAAGGCCC
         1021 TGTGGAAGCA CGGCTTCTCT TGGCCTTTCC ACAGCCCGGT GGACGCCGTG AACTAAGCTG
         1081 CCCTGACTAT TACACCATCA TAAAAAACCC CCAATGGATT GAATACATAG AGCGTTTGAA
        1141 AAATAAGTAT TTTATGAAGA AAAGGCCTTC CGGAAATG
        //
        Primer: attB2-R1
        Sequence file: RDB12840_A4Ihb.seq check
        >12840_12840_A9ErF_3_attB2-R1_C01_03_ABI08.ab1
            1 GCCGGTCAAG AGGTGGCGGG GCCCTGGGCG CTTGAGCGCG TACACCACGT CCATAGCCGT
           61 GACCGTCTTG CGCTTGGCGT GCTCGGTGTA GGTGACGGCG TCGCGGATCA CATTCTCAAG
          121 GAAAACCTTG AGGACACCGC GAGTCTCCTC GTAGATGAGA CCCGAGATGC GCTTCACGCC
          181 GCCACGCCGA GCCAACCGCC GGATAGCGGG CTTGGTAATG CCCTGGATGT TGTCGCGGAG
          241 AACCTTGCGG TGTCGCTTGG CGCCACCTTT CCCCAGACCC TTGCCGCCTT TTCCTCGTCC
          301 TGACATGGAT CCAGATCCTC CACCACCTGA ACCACCACCT CCAGAACCTC CTCCACCAGA
          361 TCCACCTCCT CCGTCGACTG GAACTTGGGA CAGAACTTGC AGCTGCTGAT GAACAGCATT
          421 AAGCTGCTCC TGAAGCTTGG CAAGATGCTG CACTCGTTCA TCCTCAGAGT CTTCAGAAGA
          481 GGCGTCCCCC GAGGAGGCCT CGCTGCTGCT TTCTCTGCTG AGGGCTTGTG CACTGTTTGT
          541 TGTAAGGTGG CATGCATGCA TACTCTCAAT AGGTTCATCG GGAATCTTGG CAAAATGTAG
          601 TTCAAAAACA TCCTGAAGGG TCCGAGCCAT CGCCACAACT TCATGATCGG GAGGATTATA
          661 CTTGTAACAG TTCATGAACA TCAGTCTGAC ATCTGCAGCA AATTCATATG CATCCTTATA
          721 TTCTTGATTG TCCATTTTCC CCTTGATCGT TCCGAGGATC CATGGGATTT TTGACAAACG
          781 TCATAGTAAG TTGTGGAGTC CCAAAAGCAT CAGCATCCAC AGGATTATAG AAGGGGGCCA
          841 TGCATAACGG CAAAATGTTT TCTTGGCAAA GCATTTCTTT TAAAAATCTC GCTTGCCAGT
          901 GCTTTTAATT TGCTCCCGTT ACTTTGAACT GTTTTTAAAA ACTTTGGTGT TGGTTGCTGA
          961 AAAGGTCTGG GCAAAAACGT TTCTTTTACT GGAATTTTTC TTTTTTACTG GGCATTGTTC
         1021 CACTTGGCTT TTTGTTTTCC TCTAAGTTGT CGGAAAGGAG AATTTCCCCT TACCTTAGAC
         1081 TTTAGACCTA ATAGGACAAT GTGTGAAGAG AGATTTAGTT GGTCGCTCTG TGCTTCTGAC
        1141 CGTACCGTCA CCCCACCCCC TCTTTCATGT TAAGATGT
        //
        Primer: BGH_rev
        Sequence file: RDB12840_A4Ihc.seq check
        >RDB12840_3_BGH_rev_C11_08.ab1
            1 CAGGTCCGGG CCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC
           61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT
          121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
          181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG
          241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
          301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
          361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
          421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
          481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC
          541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
          601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
          661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
          721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC
          781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC
          841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC
          901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC
          961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGGT AATGCCCTGG ATGTTGTCGC
         1021 GGAGAACCCT TGCGGTGTCG CTTGGCGCCA CCTTTCCCCA GACCCTTGCC GCCTTTTCTC
         1081 GTCCTGACAT GATCAGATCT CCACACCTGA ACCACAACTT CAGACTTCTT CAACCAGATC
        1141 CACCTCCTTC CGGATCGACC TGAACTTTGG C
        //
        Primer: SV40pro_F_V2
        Sequence file: RDB12840_A4Ihd.seq check
        >RDB12840_3_SV40pro_F_V2_H05_23.ab1
            1 ACAAGAGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG TATATCCATT
           61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
          121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
          181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
          241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
          301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
          361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
          421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
          481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
          541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
          601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
          661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
          721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
          781 ATTGCTGAAG AGCTTGCGGC GAATGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
          841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGACTC
          901 TGGGGTTCGA AATGACCGAC AGCGACGCCC ACTGCCATCA CGAGATTTCG ATCACGCGCT
          961 TCTATGAAGT GGGCCTCGGA TCGTTTCGGG ACGCGCTGGA TGATCTCAGC GCGGGGATCT
         1021 CATGCTGGAG TCTCGCCACC CAACCTGGTT ATGCAGCTAA TGGTACCAAT AGCATGCATC
        1081 ATTACCAATA GGCATTTTCC ACTTGCATCA
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290.
        reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
        reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.
        user_report Dancy, B.M., Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition. Chembiochem. 13 (14): 2113-2121 (2012). PMID 22961914.

        2022.10.12

        GNP_filter3_RDBDEP_html_221010.pl