RDB12840 - Histac pcDNA3.1

Resource data sheet
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Histac pcDNA3.1

For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.


Catalog number	RDB12840
Resource name	Histac pcDNA3.1
Clone info.	For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain. Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone	pcDNA3.1 (Plasmid)
Size of vector backbone	5.5kb
Gene/insert name	Mouse testis-specific bromodomain cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor	Sasaki, Kazuki \|
Other clones in our bank	mouse Brdt (NCBI Gene 114642) \| mouse Hist4h4 (NCBI Gene 320332) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.

Remarks
Please use Schedule A (GFP-A) additionally.
Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Remarks	Please use Schedule A (GFP-A) additionally. Please obtain written approval of Dr. Atsushi Miyawaki.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009)。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
付加的提供条件	提供依頼者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12840	Histac pcDNA3.1	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB12840z.seq from Depositor
Electronic file	PDF (map, protocol and/or remarks) RDB12840.pdf from Depositor

Original reference

original	Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290.

Further references such as user reports and related articles (go to bottom)

Featured content	Epigenetics clone (English text)
Featured content	Histac for visualization of acetylation of the Histon (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB12840_A4Ihp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward	RDB12840_A4Iha.seq
>RDB12840_3_CMV_Forward_B11_05.ab1 1 TCTTACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCATGGTGAG 181 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 241 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT 301 GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC 361 CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA 421 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA 481 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 541 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA 601 GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA 661 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA 721 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG 781 CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA 841 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG AATTCATGTC 901 TCTGCCAAGT CGACAAACAG CTATTGTTAA CCCCCCTCCA CCAGAGTACA TAAACACTAA 961 GAAGAGTGGG CGGGTTGACG AATCAGCTGC AGTTCCTACA GAGGGTTGTG CTGAAGGCCC 1021 TGTGGAAGCA CGGCTTCTCT TGGCCTTTCC ACAGCCCGGT GGACGCCGTG AACTAAGCTG 1081 CCCTGACTAT TACACCATCA TAAAAAACCC CCAATGGATT GAATACATAG AGCGTTTGAA 1141 AAATAAGTAT TTTATGAAGA AAAGGCCTTC CGGAAATG //
Primer: attB2-R1	RDB12840_A4Ihb.seq
>12840_12840_A9ErF_3_attB2-R1_C01_03_ABI08.ab1 1 GCCGGTCAAG AGGTGGCGGG GCCCTGGGCG CTTGAGCGCG TACACCACGT CCATAGCCGT 61 GACCGTCTTG CGCTTGGCGT GCTCGGTGTA GGTGACGGCG TCGCGGATCA CATTCTCAAG 121 GAAAACCTTG AGGACACCGC GAGTCTCCTC GTAGATGAGA CCCGAGATGC GCTTCACGCC 181 GCCACGCCGA GCCAACCGCC GGATAGCGGG CTTGGTAATG CCCTGGATGT TGTCGCGGAG 241 AACCTTGCGG TGTCGCTTGG CGCCACCTTT CCCCAGACCC TTGCCGCCTT TTCCTCGTCC 301 TGACATGGAT CCAGATCCTC CACCACCTGA ACCACCACCT CCAGAACCTC CTCCACCAGA 361 TCCACCTCCT CCGTCGACTG GAACTTGGGA CAGAACTTGC AGCTGCTGAT GAACAGCATT 421 AAGCTGCTCC TGAAGCTTGG CAAGATGCTG CACTCGTTCA TCCTCAGAGT CTTCAGAAGA 481 GGCGTCCCCC GAGGAGGCCT CGCTGCTGCT TTCTCTGCTG AGGGCTTGTG CACTGTTTGT 541 TGTAAGGTGG CATGCATGCA TACTCTCAAT AGGTTCATCG GGAATCTTGG CAAAATGTAG 601 TTCAAAAACA TCCTGAAGGG TCCGAGCCAT CGCCACAACT TCATGATCGG GAGGATTATA 661 CTTGTAACAG TTCATGAACA TCAGTCTGAC ATCTGCAGCA AATTCATATG CATCCTTATA 721 TTCTTGATTG TCCATTTTCC CCTTGATCGT TCCGAGGATC CATGGGATTT TTGACAAACG 781 TCATAGTAAG TTGTGGAGTC CCAAAAGCAT CAGCATCCAC AGGATTATAG AAGGGGGCCA 841 TGCATAACGG CAAAATGTTT TCTTGGCAAA GCATTTCTTT TAAAAATCTC GCTTGCCAGT 901 GCTTTTAATT TGCTCCCGTT ACTTTGAACT GTTTTTAAAA ACTTTGGTGT TGGTTGCTGA 961 AAAGGTCTGG GCAAAAACGT TTCTTTTACT GGAATTTTTC TTTTTTACTG GGCATTGTTC 1021 CACTTGGCTT TTTGTTTTCC TCTAAGTTGT CGGAAAGGAG AATTTCCCCT TACCTTAGAC 1081 TTTAGACCTA ATAGGACAAT GTGTGAAGAG AGATTTAGTT GGTCGCTCTG TGCTTCTGAC 1141 CGTACCGTCA CCCCACCCCC TCTTTCATGT TAAGATGT //
Primer: BGH_rev	RDB12840_A4Ihc.seq
>RDB12840_3_BGH_rev_C11_08.ab1 1 CAGGTCCGGG CCTCTAGACT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC 61 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT 121 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT 181 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG 241 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG 301 GCTGATGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT 361 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT 421 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT 481 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC 541 GCCCCAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA 601 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT 661 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA 721 CAGCTCCTCG CCCTTGCTCA CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC 781 GCCGCCGAAG CCGTACAGAG TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC 841 CGTGACCGTC TTGCGCTTGG CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC 901 AAGGAAAACC TTGAGGACAC CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC 961 GCCGCCACGC CGAGCCAACC GCCGGATAGC GGGCTTGGGT AATGCCCTGG ATGTTGTCGC 1021 GGAGAACCCT TGCGGTGTCG CTTGGCGCCA CCTTTCCCCA GACCCTTGCC GCCTTTTCTC 1081 GTCCTGACAT GATCAGATCT CCACACCTGA ACCACAACTT CAGACTTCTT CAACCAGATC 1141 CACCTCCTTC CGGATCGACC TGAACTTTGG C //
Primer: SV40pro_F_V2	RDB12840_A4Ihd.seq
>RDB12840_3_SV40pro_F_V2_H05_23.ab1 1 ACAAGAGCTT TTTTGGAGGC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG TATATCCATT 61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 781 ATTGCTGAAG AGCTTGCGGC GAATGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC 841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGACTC 901 TGGGGTTCGA AATGACCGAC AGCGACGCCC ACTGCCATCA CGAGATTTCG ATCACGCGCT 961 TCTATGAAGT GGGCCTCGGA TCGTTTCGGG ACGCGCTGGA TGATCTCAGC GCGGGGATCT 1021 CATGCTGGAG TCTCGCCACC CAACCTGGTT ATGCAGCTAA TGGTACCAAT AGCATGCATC 1081 ATTACCAATA GGCATTTTCC ACTTGCATCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290.
reference	He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
reference	Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.
user report	Dancy, B.M., Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition. Chembiochem. 13 (14): 2113-2121 (2012). PMID 22961914.

2019.05.30

GNP_filter3_RDBDEP_html_190430.pl