Resource data sheet
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pgLTR_T6 (#RDB12750)

gRNA expressing vector of HIV LTR

Alternative name T6 gRNA expressiong vector
Clone info. gRNA expressing vector. This gRNA was designed to target NF-kB binding site of HIV LTR.
Comment gRNA target sequence was cloned into the AflII site of the gRNA_cloning vector (Addgene #41824).
Vector backbone pCR-BluntII-TOPO (Plasmid)
Size of vector backbone 3.51kb
Gene/insert name Human U6 genomic DNA HIV-1 LTR genomic DNA
Depositor|Developer Koyanagi, Yoshio | Ebina, Hirotaka |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Sci Rep. 3: 2510(2013)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci Rep. 3: 2510(2013))。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB12750 pgLTR_T6 Under QC test. Please contact us. DNA solution Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pgLTR_T6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12750).

Reference section:

Ebina, H., Misawa, N., Kanemura, Y., Koyanagi, Y., Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. Sci. Rep. 3: 2510 (2013). PMID 23974631. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ebina, H., Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. Sci. Rep. 3: 2510 (2013). PMID 23974631. [link to RRC of NBRP]