Please review the QC test results indicated by icon below as well as clone information before placing your order.

pRSET-hp38deltaM107T

Bacterial expression vector of human p38delta, gate keeper mutant, His6-tagged.

Sequence (full)	RDB12369zzt01.seq compiled by us

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12369_A4B5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261) Region: 6xHis, T7 tag, Xpress tag, Insert 5' Sequence file: RDB12369_A4B5a.seq
>RDB12369_1_T7_20mer_E08_14.ab1     1 NNNNNNNNNN NNNNNNNNNN TTTGNTNACT TTAAGAAGGA NNNATACATA TGCGGGGTTC    61 TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA   121 TCTGTACGAC GATGACGATA AGGATCGATG GATCCCATCG ATGAGCCTCA TCCGGAAAAA   181 GGGCTTCTAC AAGCAGGACG TCAACAAGAC CGCCTGGGAG CTGCCCAAGA CCTACGTGTC   241 CCCGACGCAC GTCGGCAGCG GGGCCTATGG CTCCGTGTGC TCGGCCATCG ACAAGCGGTC   301 AGGGGAGAAG GTGGCCATCA AGAAGCTGAG CCGACCCTTT CAGTCCGAGA TCTTCGCCAA   361 GCGCGCCTAC CGGGAGCTGC TGCTGCTGAA GCACATGCAG CATGAGAACG TCATTGGGCT   421 CCTGGATGTC TTCACCCCAG CCTCCTCCCT GCGCAACTTC TATGACTTCT ACCTGGTGAC   481 GCCCTTCATG CAGACGGATC TGCAGAAGAT CATGGGGATG GAGTTCAGTG AGGAGAAGAT   541 CCAGTACCTG GTGTATCAGA TGCTCAAAGG CCTTAAGTAC ATCCACTCTG CTGGGGTCGT   601 GCACAGGGAC CTGAAGCCAG GCAACCTGGC TGTGAATGAG GACTGTGAAC TGAAGATTCT   661 GGATTTTGGG CTGGCGCGAC ATGCAGACGC CGAGATGACT GGCTACGTGG TGACCCGCTG   721 GTACCGAGCC CCCGAGGTGA TCCTCAGCTG GATGCACTAC AACCAGACAG TGGACATCTG   781 GTCTGTGGGC TGTATCATGG CAGAGATGCT GACAGGGAAA ACTCTGTTCN AGGGGAAAGA   841 TTACCTGNAC NAGCTGANCC AGATCCTGAA AGTGACCGGG GTGCCTGGCA CGGAGTTTGT   901 GCAGAANNCT GAACGANAAA GCGGCCNAAT CCTACATNNN GTCCCNGCCN CANANCCCCC 961 NNGANNGANT NCANNCNNCT GNNNCCNCNG NNNNNCCCCC CANNCTGNNN NNNNNNN //
Primer: T7 terminator (Pr0155) Region: insert 3' Sequence file: RDB12369_A4B5b.seq
>RDB12369_1_T7terminator_F08_17.ab1     1 NNNNNNNNNN NNNNNNNGNT NNGTNNGCNG CCGGNNNNAG CTTCGAATTC NNTGGTACCN    61 GCTGCAGGGC CCTCTAGATG AGTCCCTACA GCTTCATGCC ACTCCGGCGC CGTGAGTCCT   121 TCCGGGCAAT GGGGCTGAAG TTCACAATCT CCTTGTAGAT GTGCTGCTTC CATTCATCCA   181 CTGTGAGTTT CTCGTGTTCT AAGGAATCAT CAAACGGCTG CTGGGCCTCC GTCTCTTCCT   241 CAGGGTCCCG GAAGGGTTCA AAGAAGGGAT GGGTGAGGGC CTGCGCGGCC GTCAGGCGCT   301 TGTCCACGTC TAGCTCCAGC ATCTTCTCCA GCAGGTCCGC AGCCTGGGGG CTGGCCCGTG   361 GGAACAGCTG AGTGAAATCC TTCCTGGGGG TCTGTGGCAG GGACTGGATG TAGGATTTGG   421 CCGCTTTGTC GTTCAGCTTC TGCACAAACT CCGTGCCAGG CACCCCGGTC ACTTTCAGGA   481 TCTGGGTCAG CTGGTCCAGG TAATCTTTCC CCTTGAACAG AGTTTTCCCT GTCAGCATCT   541 CTGCCATGAT ACAGCCCACA GACCAGATGT CCACTGTCTG GTTGTAGTGC ATCCAGCTGA   601 GGATCACCTC GGGGGCTCGG TACCAGCGGG TCACCACGTA GCCAGTCATC TCGGCGTCTG   661 CATGTCGCGC CAGCCCAAAA TCCAGAATCT TCAGTTCACA GTCCTCATTC ACAGCCAGGT   721 TGCCTGGCTT CAGGTCCCTG TGCACGACCC CAGCAGAGTG GANGTACTTA AGGCCTTTGA   781 GCATCTGATA CACCAGGTAC TGGATCTTCT CCTCACTGAA CTCCNTCCCC ATGATCTTCT   841 GCAGATCCGT CTGCATGAAN GGCGTCACCA GGTAGAAGTC ATAGAAGTTG CGCANGGNGG   901 ANGCTGGGNN GAAGACATCC NGGANCCCNA TGNNGTTCTC ATGCTGNATG TGCTTCANCA   961 NCAGCAGCTC CCNGNANNNN NGCTTGNNNA ANANCTCNGN NTGAAANGGN NNGNNCANCN 1021 NCNTNNNTNG NNNNNNNNNN //
Primer: p38delta-M_R (Pr1103) Region: insert mid (M107T) Sequence file: RDB12369_A4B5c.seq
>12369_12369_B2AkF_1_p38delta-M_R_C01_03_ABI08.ab1     1 NNNNNNNNNN TTNGTCGTTN NGCTTCTGCA CAAACTCCGT GCCAGGCACC CCGGTCACTT    61 TCAGGATCTG GGTCAGCTGG TCCAGGTAAT CTTTCCCCTT GAACAGAGTT TTCCCTGTCA   121 GCATCTCTGC CATGATACAG CCCACAGACC AGATGTCCAC TGTCTGGTTG TAGTGCATCC   181 AGCTGAGGAT CACCTCGGGG GCTCGGTACC AGCGGGTCAC CACGTAGCCA GTCATCTCGG   241 CGTCTGCATG TCGCGCCAGC CCAAAATCCA GAATCTTCAG TTCACAGTCC TCATTCACAG   301 CCAGGTTGCC TGGCTTCAGG TCCCTGTGCA CGACCCCAGC AGAGTGGATG TACTTAAGGC   361 CTTTGAGCAT CTGATACACC AGGTACTGGA TCTTCTCCTC ACTGAACTCC ATCCCCATGA   421 TCTTCTGCAG ATCCGTCTGC ATGAAGGGCG TCACCAGGTA GAAGTCATAG AAGTTGCGCA   481 GGGAGGAGGC TGGGGTGAAG ACATCCAGGA GCCCAATGAC GTTCTCATGC TGCATGTGCT   541 TCAGCAGCAG CAGCTCCCGG TAGGCGCGCT TGGCGAANAT CTCGGACTGA AAGGGTCGGC   601 TCAGCTTCTT GATGGCCACC TTCTCCCCTG ACCGCTTGTC GATGGNCGAN CACACGGANC   661 NNTNNNNCCN GCTGCCGANG NGCGTCNGGG NNNCGTNNNC NNNNNNNCTN CNNNGCNGNN 721 NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl