Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3Flag-hp38delta

Expression vector of human p38delta MAPK cDNA, FLAG-tagged

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12363_A4B5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(FLAG,p38 delta 5') Sequence file: RDB12363_A4B5a.seq
>12363_12363_B2A7F_1_CMV-Forward_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNANNNGTG GGNGGTCTAT ATAAGCAGAG CTCTCTGGCT    61 AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT ATAGGGAGAC   121 CCAAGCTTGC CACCATGGAC TACAAGGACG ACGACGATAA ATCGATGAGC CTCATCCGGA   181 AAAAGGGCTT CTACAAGCAG GACGTCAACA AGACCGCCTG GGAGCTGCCC AAGACCTACG   241 TGTCCCCGAC GCACGTCGGC AGCGGGGCCT ATGGCTCCGT GTGCTCGGCC ATCGACAAGC   301 GGTCAGGGGA GAAGGTGGCC ATCAAGAAGC TGAGCCGACC CTTTCAGTCC GAGATCTTCG   361 CCAAGCGCGC CTACCGGGAG CTGCTGCTGC TGAAGCACAT GCAGCATGAG AACGTCATTG   421 GGCTCCTGGA TGTCTTCACC CCAGCCTCCT CCCTGCGCAA CTTCTATGAC TTCTACCTGG   481 TGATGCCCTT CATGCAGACG GATCTGCAGA AGATCATGGG GATGGAGTTC AGTGAGGAGA   541 AGATCCAGTA CCTGGTGTAN CAGATGCTNN NNNGCCTTAA GTACATCCAC TCTGCTGNNG   601 TCGTGCACAN GGNNCTGANN NCAGGNNNNN NGGCTGTGAA TGANGNNTGT GANCTGNNNA 661 NTCTGGNNNN TTTGGGNNNN NNNNNACNNG NNNNNNNNCN NNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: insert 3'(p38 delta 3') Sequence file: RDB12363_A4B5b.seq
>12363_12363_B2A7F_1_bGH_rev3_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNGGNNNN NNNNNNNNNA NNAGNTGGCT GGCAACTAGN NAGGCACAGT    61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTANATG   121 AGTCCCTACA GCTTCATGCC ACTCCGGCGC CGTGAGTCCT TCCGGGCAAT GGGGCTGAAG   181 TTCACAATCT CCTTGTAGAT GTGCTGCTTC CATTCATCCA CTGTGAGTTT CTCGTGTTCT   241 AAGGAATCAT CAAACGGCTG CTGGGCCTCC GTCTCTTCCT CAGGGTCCCG GAAGGGTTCA   301 AAGAAGGGAT GGGTGAGGGC CTGCGCGGCC GTCAGGCGCT TGTCCACGTC TAGCTCCAGC   361 ATCTTCTCCA GCAGGTCCGC AGCCTGGGGG CTGGCCCGTG GGAACAGCTG AGTGAAATCC   421 TTCCTGGGGG TCTGTGGCAG GGACTGGATG TAGGATTTGG CCGCTTTGTC GTTCAGCTTC   481 TGCACAAACT CCGTGCCAGG CACCCCGGTC ACTTTCAGGA TCTGGGTCAG CTGGTCCAGG   541 TAATCTTTCC CCTTGAACAG AGTTTTCCCT GTCNNCATCT CTGCCATGNN ACAGCCCANA   601 GACCANANGT CNNCTGTCTG GNTGTAGTGC ATNCNNCTGA GGATCACNTN NNGGGGCTCG 661 GTACNNNNNG GTCACCNNNN NNNCNNNNNN NNNN //
Primer: p38delta-M_R (Pr1103) Region: insert mid(p38 delta mid) Sequence file: RDB12363_A4B5c.seq
>12363_12363_B2A7F_1_p38delta-M_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGCCGCTTT GTCGTTNNGC TTCTGCACAA ACTCCGTGCC    61 AGGCACCCCG GTCACTTTCA GGATCTGGGT CAGCTGGTCC AGGTAATCTT TCCCCTTGAA   121 CAGAGTTTTC CCTGTCAGCA TCTCTGCCAT GATACAGCCC ACAGACCAGA TGTCCACTGT   181 CTGGTTGTAG TGCATCCAGC TGAGGATCAC CTCGGGGGCT CGGTACCAGC GGGTCACCAC   241 GTAGCCAGTC ATCTCGGCGT CTGCATGTCG CGCCAGCCCA AAATCCAGAA TCTTCAGTTC   301 ACAGTCCTCA TTCACAGCCA GGTTGCCTGG CTTCAGGTCC CTGTGCACGA CCCCAGCAGA   361 GTGGATGTAC TTAAGGCCTT TGAGCATCTG ATACACCAGG TACTGGATCT TCTCCTNNNN   421 NNACTCCATC CCCATGATCT TCNGNNNATC CGTCTGCATG AAGGGCATCA CCANNTANAN 481 NNNATAGAAG TTGCGCNNGG AGGANNNNGG NNNGAANANN NNNANNANNN NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: Sv40 pro_ori,NeoR Sequence file: RDB12363_A4B5d.seq
>12363_12363_B2A7F_1_SV40pro_ori_F_C03_09_ABI24.ab1     1 NNNNNCNGNN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCNATC ANGATGATCT   601 GGACGAAGAG CATCANGGGC TCGCGCCNGC CNAACTGNTC GCCNNGCTNN ANGCGCNNAT   661 GNCCGANNGC GANNNNCTCN TCNNGACCNN NGGNNNNGNC NGNNNNNNNA NNNNNNNNNG 721 NNNGNNAANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl