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pBR-lacZ alpha

Plasmid clone for quantitative assay for genome editing

Catalog number RDB12261
Resource name pBR-lacZ alpha
Clone info. Plasmid clone for quantitative assay for genome editing at endogenous genomic loci.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers Amp^r
Gene/insert name E. coli lacZ genomic DNA
Depositor|Developer Hisano, Yu |
Other clones in our bank

External Database
E. coli lacZ

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Biology Open, 2, 363-367 (2013)).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biology Open, 2, 363-367 (2013))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をご参照ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12261 pBR-lacZ alpha DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hisano, Y., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open., 2: 363-367 (2013). PMID 23616919.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12261_A6Hup1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB12261_A6Hua.seq check
          >12261_12261_A6Hu_1_T7_20bp_D08_11_ABI24.ab1
              1 CGTAACATGG GCGCGTCGAG TCGGCGGTAT CGATAAGCGT GTGATATCGA GATTCCTGCA
             61 GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT
            121 CCCTTTAGTG AGGGTTAATT TCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT
            181 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG
            241 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT
            301 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC TAGCAGCACG CCATAGTGAC TGGCGATGCT
            361 GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC
            421 TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA
            481 CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT
            541 GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT
            601 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA
            661 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
            721 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
            781 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
            841 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
            901 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC CGAAAGAACG
            961 TTTTTCCCAA TGATGAGCAC TTTTTAAAGT TTCTGCTATG TGGGCGCGGG TATTTATCCC
           1021 GTGTTTGACG CCGGGCAAGA GCAACTCGGT TCGGCGCATA CACTACCTCA GATTGACCTT
          1081 GGTGAAGTAC CTCACCCAGG TTCCCACAGG A
          //
          Primer: T3
          Sequence file: RDB12261_A6Hub.seq check
          >12261_12261_A6Hu_1_T3_G08_20_ABI24.ab1
              1 CAGGCTCACG CGGTGGCGGC CGCTCTAGAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA
             61 TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT TCGCCCTATA
            121 GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG
            181 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
            241 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG
            301 CGCCCTGTAG CGAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG
            361 GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT
            421 TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC
            481 TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA
            541 ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGACCGATGC
            601 CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG
            661 CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT
            721 GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG
            781 CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC
            841 GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT
            901 TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCCA TTATGATTCT TCTCGCTTTC
            961 CCGGCGGCAT CGGGATGCCC GCGTTTGCAG CCCATGCTGT CCAGGGCAGT AGATGACGAA
           1021 CCAATCAGGA CAGGCCTCAA TGATCGCCTC CGCGCCTCTA CAGTCTACCT TTCGAATCAT
          1081 TGGCAAACCG CCTGG
          //
          Primer: ColE1ori_F
          Sequence file: RDB12261_A6Huc.seq check
          >12261_12261_A6Hu_1_ColE1ori_F_B09_06_ABI24.ab1
              1 TTCTTTTATC TGATCGTCGC ACCGTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
             61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
            121 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
            181 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
            241 CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG
            301 GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA
            361 GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC
            421 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
            481 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
            541 GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG
            601 TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA
            661 GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG
            721 ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGG
            781 TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT
            841 GGATGCGGCG GGACCAGAGA AAAATCACTC AGGTCAATGC CAGCGCTTCG TTTAATACAG
            901 ATGTAAGTGT TCCACAGGGT AGCCCAGCAG CATCCTGCGA TGCAGATCCG GGAACATATG
            961 GGTGCAGGCG CTGAACTTTC GGCGTTTCAG ACTTTACGGA AACACGGAAC GAGGACATTC
           1021 ATGTTGGTGC TCAGTTCGCA GACGTTTTGC AGCAGCAGCT CGCTCAACGG ATCGCTTCGG
          1081 CA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hisano, Y., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open., 2: 363-367 (2013). PMID 23616919.
          reference Hisano, Y., Genome editing using artificial site-specific nucleases in zebrafish. Dev. Growth Differ. 56 (1): 26-33 (2014). PMID 24117409.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl