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pBR-lacZ alpha

Plasmid clone for quantitative assay for genome editing

Catalog number RDB12261
Resource name pBR-lacZ alpha
Clone info. Plasmid clone for quantitative assay for genome editing at endogenous genomic loci.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers Amp^r
Gene/insert name E. coli lacZ genomic DNA
Depositor Hisano, Yu |
 
Other clones in our bank E. coli lacZ (KEGG orthology K01190) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Biology Open, 2, 363-367 (2013)).
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RDB12261 pBR-lacZ alpha DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

original Hisano, Y., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open., 2: 363-367 (2013). PMID 23616919.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12261_A6Hup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB12261_A6Hua.seq
>12261_12261_A6Hu_1_T7_20bp_D08_11_ABI24.ab1
    1 CGTAACATGG GCGCGTCGAG TCGGCGGTAT CGATAAGCGT GTGATATCGA GATTCCTGCA
   61 GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT
  121 CCCTTTAGTG AGGGTTAATT TCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT
  181 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG
  241 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT
  301 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC TAGCAGCACG CCATAGTGAC TGGCGATGCT
  361 GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC
  421 TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA
  481 CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT
  541 GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT
  601 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA
  661 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
  721 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
  781 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
  841 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
  901 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC CGAAAGAACG
  961 TTTTTCCCAA TGATGAGCAC TTTTTAAAGT TTCTGCTATG TGGGCGCGGG TATTTATCCC
 1021 GTGTTTGACG CCGGGCAAGA GCAACTCGGT TCGGCGCATA CACTACCTCA GATTGACCTT
1081 GGTGAAGTAC CTCACCCAGG TTCCCACAGG A
//
Primer: T3 RDB12261_A6Hub.seq
>12261_12261_A6Hu_1_T3_G08_20_ABI24.ab1
    1 CAGGCTCACG CGGTGGCGGC CGCTCTAGAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA
   61 TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT TCGCCCTATA
  121 GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG
  181 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG
  241 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG
  301 CGCCCTGTAG CGAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG
  361 GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT
  421 TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC
  481 TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA
  541 ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGACCGATGC
  601 CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG
  661 CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT
  721 GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG
  781 CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC
  841 GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT
  901 TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCCA TTATGATTCT TCTCGCTTTC
  961 CCGGCGGCAT CGGGATGCCC GCGTTTGCAG CCCATGCTGT CCAGGGCAGT AGATGACGAA
 1021 CCAATCAGGA CAGGCCTCAA TGATCGCCTC CGCGCCTCTA CAGTCTACCT TTCGAATCAT
1081 TGGCAAACCG CCTGG
//
Primer: ColE1ori_F RDB12261_A6Huc.seq
>12261_12261_A6Hu_1_ColE1ori_F_B09_06_ABI24.ab1
    1 TTCTTTTATC TGATCGTCGC ACCGTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
   61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
  121 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
  181 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
  241 CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG
  301 GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA
  361 GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC
  421 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
  481 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
  541 GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG
  601 TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA
  661 GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG
  721 ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGG
  781 TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT
  841 GGATGCGGCG GGACCAGAGA AAAATCACTC AGGTCAATGC CAGCGCTTCG TTTAATACAG
  901 ATGTAAGTGT TCCACAGGGT AGCCCAGCAG CATCCTGCGA TGCAGATCCG GGAACATATG
  961 GGTGCAGGCG CTGAACTTTC GGCGTTTCAG ACTTTACGGA AACACGGAAC GAGGACATTC
 1021 ATGTTGGTGC TCAGTTCGCA GACGTTTTGC AGCAGCAGCT CGCTCAACGG ATCGCTTCGG
1081 CA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hisano, Y., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open., 2: 363-367 (2013). PMID 23616919.
reference Hisano, Y., Genome editing using artificial site-specific nucleases in zebrafish. Dev. Growth Differ. 56 (1): 26-33 (2014). PMID 24117409.

2018.09.03

GNP_filter3_RDBDEP_html_180823.pl