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Flag-Chk1 (1-365) K38M mutant (#RDB12230)

Mammalian expression clone of rat Chk1 (1-365) K38M mutant, FLAG-tagged

Alternative name rat CHEK (1-365) K38M mutant
Clone info. Mammalian expression clone of rat Chk1 (1-365) K38M mutant, FLAG-tagged.
Comment Xpress-tag and 6xHis-tag have been removed from the vector backbone. Expression was observed by the depositor with the Western blotting and immunohistostaining using anti-FLAG antibody.
Vector backbone pcDNA4/HisMax-TOPO (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Rat Chk1 cDNA
Depositor|Developer Takeda, Kenji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB12230 Flag-Chk1 (1-365) K38M mutant Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The Flag-Chk1 (1-365) K38M mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12230).

Reference section:

Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]