Please review the QC test results indicated by icon below as well as clone information before placing your order.

SNAP-RALT S250A mutant

Mammalian expression clone of rat RALT S250A mutant

Sequence (full)	RDB12228zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12228_B1Bcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB12228_B1Bca.seq
>D01392A4_B1Bc_3_CMV-Forward_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG NNNNNNNGGG CGGTANGCGT GTACGGTGGG ANGTCTATAT    61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC   121 GACTCACTAT AGGGAGACCC AAGCTTGGTA CCGAGCTCGG ATCGATATCG AATTCACCAT   181 GGACAAAGAC TGCGAAATGA AGCGCACCAC CCTGGATAGC CCTCTGGGCA AGCTGGAACT   241 GTCTGGGTGC GAACAGGGCC TGCACGAGAT CAAGCTGCTG GGCAAAGGAA CATCTGCCGC   301 CGACGCCGTG GAAGTGCCTG CCCCAGCCGC CGTGCTGGGC GGACCAGAGC CACTGATGCA   361 GGCCACCGCC TGGCTCAACG CCTACTTTCA CCAGCCTGAG GCCATCGAGG AGTTCCCTGT   421 GCCAGCCCTG CACCACCCAG TGTTCCAGCA GGAGAGCTTT ACCCGCCAGG TGCTGTGGAA   481 ACTGCTGAAA GTGGTGAAGT TCGGAGAGGT CATCAGCTAC CAGCAGCTGG CCGCCCTGGC   541 CGGCAATCCC GCCGCCACCG CCGCCGTGAA AACCGCCCTG AGCGGAAATC CCGTGCCCAT   601 TCTGATCCCC TGCCACCGGG TGGTGTCTAG CTCTGGCGCC GTGGGGGGCT ACGAGGGCGG   661 GCTCGCCGTG AAAGAGTGGC TGCTGGCCCA CGAGGGCCAC AGACTGGGCA AGCCTGGGCT   721 GGGTCCTGCA GGTATAGGCG CGCCAGGAAT GTCAACAGCA GGAGTTGCTG CTCCGGATAT   781 TCGAGTCCCA TTAAAAACTG GATTTCTCCA TAATGGCCAG GCTTTGGNGA ATATGAAGAC   841 CTGCTGGGGC AGTCGCAATG ANTTTGAAAA GAANTTTTTT AAATATCGAT CCNATANNCA   901 TGGCCNACAN TCCGAACTCC CNNGCTCCGG ANCATCTNAN NACCNNNNNA NNNNCTTCNN 961 CNNCNGNNNC NNGNNNNNGN NNNNNNNNTN NNNNNNNNN //
Primer: gene33-B_F1 (Pr0948) Region: insert 3', intron Sequence file: RDB12228_B1Bcb.seq
>D01392A4_B1Bc_3_gene33-B_F1_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNAGCTGGCC TCNNGAANGA AAANGGANGT GGGCGGCCAC    61 GGGAAGCGCA AACACTTATC CTACGTGGTT TCTCCGTAGT CGAGTGAGGC GGCCGCATAG   121 ATAACTGATC CAGTGTGCTG GAATTAATTC GCTGTCTGCG AGGGCCAGCT GTTGGGGTGA   181 GTACTCCCTC TCAAAAGCGG GCATGACTTC TGCGCTAAGA TTGTCAGTTT CCAAAAACGA   241 GGAGGATTTG ATATTCACCT GGCCCGCGGT GATGCCTTTG AGGGTGGCCG CGTCCATCTG   301 GTCAGAAAAG ACAATCTTTT TGTTGTCAAG CTTGAGGTGT GGCAGGCTTG AGATCTGGCC   361 ATACACTTGA GTGACAATGA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG   421 GTCCAACTGC AGGTCGAGCA TGCATCTAGG GCGGCCAATT CCGCCCCTCT CCCCNNNNNN   481 CCTCTCCCTC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC   541 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA   601 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT   661 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC   721 AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG   781 CGGCCNAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGNGCCNCGT   841 TGTGAGTTGG ATAGTTGTGN AAAGANTCAA ATGGCTCTCC TCNAGCGNAT TCAACAAGGG   901 GCTGANNNAN GNCNAGAANN NNACCCCATN GNATGGGNNC TGATCNGGGG NNNNNNNGCA 961 NATNCNTNAC NTGTNNTTAN NNNNNNNTNA AAAAANNNNN NNNNN //
Primer: gene33-M_F1 (Pr0947) Region: insert mid (S250A) Sequence file: RDB12228_B1Bcc.seq
>D01392A4_B1Bc_3_gene33-M_F1_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NCTTAGCTGT GCNTCTGANC NGAACAGAGT TGTTCCAGAT    61 CCGAACCCTC CCCCGCCTCA AAGCCATCGC AGATTAAGGA GGTCCCACGC AGGACCAGCC   121 GGGTCGTTTA ACAAGCCAGC CATCCGGATA TCTAGCTGCA CACACAGAGC CTCTCCTAGC   181 TCGGATGAAG ACAAGCCTGA GATCCCGCCC CGGGTTCCTA TACCTCCCAG GCCAGCCAAG   241 CCAGACTACA GAAGGTGGTC AGCAGAAGTG ACCTCTAACA CATACAGTGA TGAAGATAGG   301 CCTCCCAAAG TCCCCCCGAG AGAACCTTTG TCTCGGAGTA ACTCCCGTAC TCCAAGTCCT   361 AAAAGCCTTC CGTCTTACCT CAATGGGGTC ATGCCTCCAA CACAGAGCTT TGCCCCCGAC   421 CCCAAGTATG TCAGCAGCAA AGCCCCGCAG AGACAGAGCA GCGAAGGGTC TGCCAAGGCC   481 CCCTGCATCC TGCCCATCAT TGAGAATGGG AAGAAGGTCA GCTCCACGCA TTATTACCTA   541 CTACCTGAGA GACCGCCATA CCTTGACAAA TATGAGAAGT ATTTTAGGGA AGCAGAAGAA   601 GCAAACCCAA GCACCCAGAT TCAGCCATTA CCTGCTGCCT GTGGTATGGT CTCTGCCACA   661 GACAAGCTGG CCTCCAGAAT GAAAATGGAT GTGGGCGGCC ACGGGAAGCG CAAACACTTA   721 TCCTACGTGG TTTCTCCGTA GTCGAGTGAN GCGGCCGCAT AGATAACTGA TCCAGTGTGC   781 TGGAATTAAT TCGCTGTCTG CGAGGGCCAG CTGTTGNGGT GAGTACTCCC TCTCAAAAGC   841 GGGCATGACT TCTGCGCTAA GATTGTCAGT TTCCNAAAAC GANGANGANT TGATATTCAC   901 CTGNCCCGCG GNGANGCCTT TNANGGTGGN CGCGNCCATC NGGTCAGAAA AGANNATCNT 961 TTTTGNNGTC NNGCTTGNNN GNNNNNNNNT NNNNNNNNNG NNNNNNNNNN //
Primer: SNAP_3_F (Pr0948) Region: insert mid Sequence file: RDB12228_B1Bcd.seq
>D01392A4_B1Bc_3_SNAP_3_F_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGN NNNNNNNNNG GNNANCCNGG GCNGGGTCCT    61 GCAGGTATAG GCGCGCCAGG AATGTCAACA GCAGGAGTTG CTGCTCCGGA TATTCGAGTC   121 CCATTAAAAA CTGGATTTCT CCATAATGGC CAGGCTTTGG GGAATATGAA GACCTGCTGG   181 GGCAGTCGCA ATGAGTTTGA AAAGAATTTT TTAAATATCG ATCCAATAAC CATGGCCTAC   241 AATCCGAACT CCCCTGCTCC GGAGCATCTA ACAACCCTCG GATGTGCTTC TCCGCCCGCT   301 CCAGGGAGCG GTCACTTCTT TGCAGAGCGT GGTCCATCTC CAAAGTCAAG CTTGCCACCT   361 CTTGTTACCC CACCAAGTGA AAGCTCGGGA CAGCGTGAAG AGGATCAAGT TCTGTGTGGT   421 TTTAAGAAAC TCTCAGTGAA TGGGGTGTGC GCTTCCACAC CTCCACTTAC ACCCATTCAA   481 AGCTGCTCTT CCCCGTTTCC CTGTGCAGCT CCCTGTGATC GCAGTTCCCG ACCGCTCCCA   541 CCATTGCCCA TCTCTGAGGA CCCATCTCTG GATGAGGCCG ACTGTGAGGT GGAATTTCTA   601 ACCAGTGCAG ATACAGACTT CCTTTTAGAA GACTGCGTGC CTTCCGATTT CAAATACGAT   661 GTTCCTGGCA GGCGAAGCTT CCGTGGGTGC GGACAGATCA ATTATGCGTA TTTTGACAGC   721 CCAACTGTTT CTGTGGCAGA TCTTAGCTGT GCATCTGACC AGAACAGAGT TGTTCCAGAT   781 CCGAACCCTC CCCCGCCTCA AAGCCATCGN NNNNTANNGA GGTCCCACGC ANGANCAGCC   841 NGGTCGTTTA ACAAGCCAGC CATCCGGATA TCTANCTGCA CACACAGAGC CTCTCCTAGC   901 TCGNANGAAG ACAANCCTGA NATNCCGCCC CCGGGTNNCT ATANCTCCCN NNNNAGCCNA 961 NGNNNNANTA CAGAANNNGG NCAGCANNAG NGNNCNNNNN NNCNTACNNN NNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES, NeoR Sequence file: RDB12228_B1Bce.seq
>D01392A4_B1Bc_3_IRES-F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN GTCGANGNNN NNNNNCGTCT AGGCCCCCCG AACCACGGGG    61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AAGCTTGCCA CAACCCGGGA TAATTCCTGC   121 AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG   181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC   241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG   301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT   361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG   421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT   481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA   541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA   601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA   661 GGCGCGCATG CCCGACGGCG ATGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA   721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC   781 GGANCGCTAT CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGANC TTGGCGGCGA   841 ATGGGCTGAC CGCTTCCTCN TGCTTTACGG TATCGCCGCT CCCGANTCGC ANCGCATCGC   901 CTTCTATCGC CNTCTNGANN ANTNCTTCNN ANGGGNNCNN NTCTCNANAN NNNNGATCAT 961 AATCNNNNNT NNCNNCNNTN NNNNNNNNTT NNNNNNNNTT NNNNAANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl