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SNAP-RALT S250A mutant (#RDB12228)

Mammalian expression clone of rat RALT S250A mutant

Alternative name SNAP-tagged Gene-33 S250A mutant
Clone info. Mammalian expression clone of rat RALT S250A mutant, SNAP-tagged..
Comment Expression was observed by the depositor with the fluorescent detection using SNAP-tag substrate.
Vector backbone pSNAP-tag(m) (Plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r
Gene/insert name Rat RALT cDNA
Depositor|Developer Takeda, Kenji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12228 SNAP-RALT S250A mutant DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The SNAP-RALT S250A mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12228).

Reference section:

Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12228_B1Bcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'
Sequence file: RDB12228_B1Bca.seq check
>D01392A4_B1Bc_3_CMV-Forward_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNNNNNNGGG CGGTANGCGT GTACGGTGGG ANGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTTGGTA CCGAGCTCGG ATCGATATCG AATTCACCAT
  181 GGACAAAGAC TGCGAAATGA AGCGCACCAC CCTGGATAGC CCTCTGGGCA AGCTGGAACT
  241 GTCTGGGTGC GAACAGGGCC TGCACGAGAT CAAGCTGCTG GGCAAAGGAA CATCTGCCGC
  301 CGACGCCGTG GAAGTGCCTG CCCCAGCCGC CGTGCTGGGC GGACCAGAGC CACTGATGCA
  361 GGCCACCGCC TGGCTCAACG CCTACTTTCA CCAGCCTGAG GCCATCGAGG AGTTCCCTGT
  421 GCCAGCCCTG CACCACCCAG TGTTCCAGCA GGAGAGCTTT ACCCGCCAGG TGCTGTGGAA
  481 ACTGCTGAAA GTGGTGAAGT TCGGAGAGGT CATCAGCTAC CAGCAGCTGG CCGCCCTGGC
  541 CGGCAATCCC GCCGCCACCG CCGCCGTGAA AACCGCCCTG AGCGGAAATC CCGTGCCCAT
  601 TCTGATCCCC TGCCACCGGG TGGTGTCTAG CTCTGGCGCC GTGGGGGGCT ACGAGGGCGG
  661 GCTCGCCGTG AAAGAGTGGC TGCTGGCCCA CGAGGGCCAC AGACTGGGCA AGCCTGGGCT
  721 GGGTCCTGCA GGTATAGGCG CGCCAGGAAT GTCAACAGCA GGAGTTGCTG CTCCGGATAT
  781 TCGAGTCCCA TTAAAAACTG GATTTCTCCA TAATGGCCAG GCTTTGGNGA ATATGAAGAC
  841 CTGCTGGGGC AGTCGCAATG ANTTTGAAAA GAANTTTTTT AAATATCGAT CCNATANNCA
  901 TGGCCNACAN TCCGAACTCC CNNGCTCCGG ANCATCTNAN NACCNNNNNA NNNNCTTCNN
961 CNNCNGNNNC NNGNNNNNGN NNNNNNNNTN NNNNNNNNN
//
Primer: gene33-B_F1 (Pr0948)
Region: insert 3', intron
Sequence file: RDB12228_B1Bcb.seq check
>D01392A4_B1Bc_3_gene33-B_F1_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNAGCTGGCC TCNNGAANGA AAANGGANGT GGGCGGCCAC
   61 GGGAAGCGCA AACACTTATC CTACGTGGTT TCTCCGTAGT CGAGTGAGGC GGCCGCATAG
  121 ATAACTGATC CAGTGTGCTG GAATTAATTC GCTGTCTGCG AGGGCCAGCT GTTGGGGTGA
  181 GTACTCCCTC TCAAAAGCGG GCATGACTTC TGCGCTAAGA TTGTCAGTTT CCAAAAACGA
  241 GGAGGATTTG ATATTCACCT GGCCCGCGGT GATGCCTTTG AGGGTGGCCG CGTCCATCTG
  301 GTCAGAAAAG ACAATCTTTT TGTTGTCAAG CTTGAGGTGT GGCAGGCTTG AGATCTGGCC
  361 ATACACTTGA GTGACAATGA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG
  421 GTCCAACTGC AGGTCGAGCA TGCATCTAGG GCGGCCAATT CCGCCCCTCT CCCCNNNNNN
  481 CCTCTCCCTC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC
  541 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA
  601 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT
  661 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC
  721 AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG
  781 CGGCCNAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGNGCCNCGT
  841 TGTGAGTTGG ATAGTTGTGN AAAGANTCAA ATGGCTCTCC TCNAGCGNAT TCAACAAGGG
  901 GCTGANNNAN GNCNAGAANN NNACCCCATN GNATGGGNNC TGATCNGGGG NNNNNNNGCA
961 NATNCNTNAC NTGTNNTTAN NNNNNNNTNA AAAAANNNNN NNNNN
//
Primer: gene33-M_F1 (Pr0947)
Region: insert mid (S250A)
Sequence file: RDB12228_B1Bcc.seq check
>D01392A4_B1Bc_3_gene33-M_F1_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN NCTTAGCTGT GCNTCTGANC NGAACAGAGT TGTTCCAGAT
   61 CCGAACCCTC CCCCGCCTCA AAGCCATCGC AGATTAAGGA GGTCCCACGC AGGACCAGCC
  121 GGGTCGTTTA ACAAGCCAGC CATCCGGATA TCTAGCTGCA CACACAGAGC CTCTCCTAGC
  181 TCGGATGAAG ACAAGCCTGA GATCCCGCCC CGGGTTCCTA TACCTCCCAG GCCAGCCAAG
  241 CCAGACTACA GAAGGTGGTC AGCAGAAGTG ACCTCTAACA CATACAGTGA TGAAGATAGG
  301 CCTCCCAAAG TCCCCCCGAG AGAACCTTTG TCTCGGAGTA ACTCCCGTAC TCCAAGTCCT
  361 AAAAGCCTTC CGTCTTACCT CAATGGGGTC ATGCCTCCAA CACAGAGCTT TGCCCCCGAC
  421 CCCAAGTATG TCAGCAGCAA AGCCCCGCAG AGACAGAGCA GCGAAGGGTC TGCCAAGGCC
  481 CCCTGCATCC TGCCCATCAT TGAGAATGGG AAGAAGGTCA GCTCCACGCA TTATTACCTA
  541 CTACCTGAGA GACCGCCATA CCTTGACAAA TATGAGAAGT ATTTTAGGGA AGCAGAAGAA
  601 GCAAACCCAA GCACCCAGAT TCAGCCATTA CCTGCTGCCT GTGGTATGGT CTCTGCCACA
  661 GACAAGCTGG CCTCCAGAAT GAAAATGGAT GTGGGCGGCC ACGGGAAGCG CAAACACTTA
  721 TCCTACGTGG TTTCTCCGTA GTCGAGTGAN GCGGCCGCAT AGATAACTGA TCCAGTGTGC
  781 TGGAATTAAT TCGCTGTCTG CGAGGGCCAG CTGTTGNGGT GAGTACTCCC TCTCAAAAGC
  841 GGGCATGACT TCTGCGCTAA GATTGTCAGT TTCCNAAAAC GANGANGANT TGATATTCAC
  901 CTGNCCCGCG GNGANGCCTT TNANGGTGGN CGCGNCCATC NGGTCAGAAA AGANNATCNT
961 TTTTGNNGTC NNGCTTGNNN GNNNNNNNNT NNNNNNNNNG NNNNNNNNNN
//
Primer: SNAP_3_F (Pr0948)
Region: insert mid
Sequence file: RDB12228_B1Bcd.seq check
>D01392A4_B1Bc_3_SNAP_3_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGN NNNNNNNNNG GNNANCCNGG GCNGGGTCCT
   61 GCAGGTATAG GCGCGCCAGG AATGTCAACA GCAGGAGTTG CTGCTCCGGA TATTCGAGTC
  121 CCATTAAAAA CTGGATTTCT CCATAATGGC CAGGCTTTGG GGAATATGAA GACCTGCTGG
  181 GGCAGTCGCA ATGAGTTTGA AAAGAATTTT TTAAATATCG ATCCAATAAC CATGGCCTAC
  241 AATCCGAACT CCCCTGCTCC GGAGCATCTA ACAACCCTCG GATGTGCTTC TCCGCCCGCT
  301 CCAGGGAGCG GTCACTTCTT TGCAGAGCGT GGTCCATCTC CAAAGTCAAG CTTGCCACCT
  361 CTTGTTACCC CACCAAGTGA AAGCTCGGGA CAGCGTGAAG AGGATCAAGT TCTGTGTGGT
  421 TTTAAGAAAC TCTCAGTGAA TGGGGTGTGC GCTTCCACAC CTCCACTTAC ACCCATTCAA
  481 AGCTGCTCTT CCCCGTTTCC CTGTGCAGCT CCCTGTGATC GCAGTTCCCG ACCGCTCCCA
  541 CCATTGCCCA TCTCTGAGGA CCCATCTCTG GATGAGGCCG ACTGTGAGGT GGAATTTCTA
  601 ACCAGTGCAG ATACAGACTT CCTTTTAGAA GACTGCGTGC CTTCCGATTT CAAATACGAT
  661 GTTCCTGGCA GGCGAAGCTT CCGTGGGTGC GGACAGATCA ATTATGCGTA TTTTGACAGC
  721 CCAACTGTTT CTGTGGCAGA TCTTAGCTGT GCATCTGACC AGAACAGAGT TGTTCCAGAT
  781 CCGAACCCTC CCCCGCCTCA AAGCCATCGN NNNNTANNGA GGTCCCACGC ANGANCAGCC
  841 NGGTCGTTTA ACAAGCCAGC CATCCGGATA TCTANCTGCA CACACAGAGC CTCTCCTAGC
  901 TCGNANGAAG ACAANCCTGA NATNCCGCCC CCGGGTNNCT ATANCTCCCN NNNNAGCCNA
961 NGNNNNANTA CAGAANNNGG NCAGCANNAG NGNNCNNNNN NNCNTACNNN NNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES, NeoR
Sequence file: RDB12228_B1Bce.seq check
>D01392A4_B1Bc_3_IRES-F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN GTCGANGNNN NNNNNCGTCT AGGCCCCCCG AACCACGGGG
   61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AAGCTTGCCA CAACCCGGGA TAATTCCTGC
  121 AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
  181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
  241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
  301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
  361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
  421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
  481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
  541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
  601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
  661 GGCGCGCATG CCCGACGGCG ATGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
  721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC
  781 GGANCGCTAT CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGANC TTGGCGGCGA
  841 ATGGGCTGAC CGCTTCCTCN TGCTTTACGG TATCGCCGCT CCCGANTCGC ANCGCATCGC
  901 CTTCTATCGC CNTCTNGANN ANTNCTTCNN ANGGGNNCNN NTCTCNANAN NNNNGATCAT
961 AATCNNNNNT NNCNNCNNTN NNNNNNNNTT NNNNNNNNTT NNNNAANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]