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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

SNAP-RALT (#RDB12227)

Mammalian expression clone of rat RALT.

Alternative name SNAP-tagged Gene-33
Clone info. Mammalian expression clone of rat RALT, SNAP-tagged. This clone contains a cDNA fragment corresponding to the 274 to 1649nt of X07266.1.
Comment Expression was observed by the depositor with the fluorescent detection using SNAP-tag substrate. Known differences (at least) : Substitutions with reference to X07266.1 are A to C 295nt (Gln to Pro), T to C 433nt (Leu to Pro), T to C 483nt (Ser to Pro), C to A 545nt (Pro, synonym), T to C 556nt (Ile to Thr), A to G 971nt (Pro, synonym), T to C 1013nt (Ser, synonym), C to T 1259nt (Thr, synonym), and T to C 1354nt (Leu to Pro).
Vector backbone pSNAP-tag(m) (Plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r
Gene/insert name Rat RALT cDNA
Depositor|Developer Takeda, Kenji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。

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RDB12227 SNAP-RALT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The SNAP-RALT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12227).

Reference section:

Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12227_B0Llp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SNAP_3_F (Pr0231)
Region: SNAP-Tag, insert 5'
Sequence file: RDB12227_B0Lla.seq check
>12227_12227_B0Ll_SNAP_3_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTGGGCA GCCTGGGCTG GGTCCTGCAG GTATAGGCGC
   61 GCCAGGAATG TCAACAGCAG GAGTTGCTGC TCCGGATATT CGAGTCCCAT TAAAAACTGG
  121 ATTTCTCCAT AATGGCCAGG CTTTGGGGAA TATGAAGACC TGCTGGGGCA GTCGCAATGA
  181 GTTTGAAAAG AATTTTTTAA ATATCGATCC AATAACCATG GCCTACAATC CGAACTCCCC
  241 TGCTCCGGAG CATCTAACAA CCCTCGGATG TGCTTCTCCG CCCGCTCCAG GGAGCGGTCA
  301 CTTCTTTGCA GAGCGTGGTC CATCTCCAAA GTCAAGCTTG CCACCTCTTG TTACCCCACC
  361 AAGTGAAAGC TCGGGACAGC GTGAAGAGGA TCAAGTTCTG TGTGGTTTTA AGAAACTCTC
  421 AGTGAATGGG GTGTGCGCTT CCACACCTCC ACTTACACCC ATTCAAAGCT GCTCTTCCCC
  481 GTTTCCCTGT GCAGCTCCCT GTGATCGCAG TTCCCGACCG CTCCCACCAT TGCCCATCTC
  541 TGANGACCCA TCTCTGGATG ANGCCGACTG TGANGTGGAA TTTCTAACCA GTGCAGATAC
  601 AGACTTCCTT TTANAAGANT GCGTGCCTTC CNATTTCNAA TACNATGTTC CTGGCANGCG
  661 AANCTTNCGT GGGTGCNGNN AGNNCAATTA NGCNNANTTN NNNNGCCNNA CNGNTNNNNN
721 NNNNN
//
Primer: chimeric in-H_R (Pr0942)
Region: insert 3'
Sequence file: RDB12227_B0Llb.seq check
>12227_12227_B0Ll_chimeric in-H_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNGG CCCTCGCAGA CAGCGAATTA ATTCCAGCAC ACTGGATCAG
   61 TTATCTATGC GGCCGCCTCA CTCGACTACG GAGAAACCAC GTAGGATAAG TGTTTGCGCT
  121 TCCCGTGGCC GCCCACATCC ATTTTCATTC TGGAGGCCAG CTTGTCTGTG GCAGAGACCA
  181 TACCACAGGC AGCAGGTAAT GGCTGAATCT GGGTGCTTGG GTTTGCTTCT TCTGCTTCCC
  241 TAAAATACTT CTCATATTTG TCAAGGTATG GCGGTCTCTC AGGTAGTAGG TAATAATGCG
  301 TGGAGCTGAC CTTCTTCCCA TTCTCAATGA TGGGCAGGAT GCAGGGGGCC TTGGCAGACC
  361 CTTCGCTGCT CTGTCTCTGC GGGGCTTTGC TGCTGACATA CTTGGGGTCG GGGGCAAAGC
  421 TCTGTGTTGG AGGCATGACC CCATTGAGGT AAGACGGAAG GCTTTTAGGA CTTGGAGTAC
  481 GGGAGTTACT CCGAGACAAA GGTTCTCTCG GGGGGACTTT GGGAGGCCTA TCTTCATCAC
  541 TGTATGTGTT AGAGGTCACT TCTGCTGACC ACCTTCTGTA GTCTGGCTTG NCTGGCCTGG
  601 GANGTATAGG AACCCGGGGC GGGNNCTCAN GCTTGTCTTC NTCCNAGCTA NGANANGCTC
  661 TGTGTGTGCA GCTAGATATC CNGNNGNNTG GCTTGNTNAA CNANCCNGCN NGNCCNGANN
721 NGGNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, SNAP-Tag
Sequence file: RDB12227_B0Llc.seq check
>12227_12227_B0Ll_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGC ANTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TTGGTACCGA GCTCGGATCG ATATCGAATT CACCATGGAC
  181 AAAGACTGCG AAATGAAGCG CACCACCCTG GATAGCCCTC TGGGCAAGCT GGAACTGTCT
  241 GGGTGCGAAC AGGGCCTGCA CGAGATCAAG CTGCTGGGCA AAGGAACATC TGCCGCCGAC
  301 GCCGTGGAAG TGCCTGCCCC AGCCGCCGTG CTGGGCGGAC CAGAGCCACT GATGCAGGCC
  361 ACCGCCTGGC TCAACGCCTA CTTTCACCAG CCTGAGGCCA TCGAGGAGTT CCCTGTGCCA
  421 GCCCTGCACC ACCCAGTGTT CCAGCAGGAG AGCTTTACCC GCCAGGTGCT GTGGAAACTG
  481 CTGAAAGTGG TGAAGTTCGG AGAGGTCATC AGCTACCAGC AGCTGGCCGC CCTGGCCGGC
  541 AATCCCGCCG CCACCGCCGC CGTGAAAACC GCCCTGANCG GAAATCCCGT GCCCATTCTG
  601 ATCCCCTGCC ACCGGGTGGT GTCTAGCTCT GGCGCCGTGG GGGGCTACNA NGGCGGGCTC
  661 GCCGTGAAAN ANTGGCTGCT GGNCNNCNNN GGNNACNGAN TGGGNNNNCN NNGNNTGGGT
721 NNNGCNNNNN NNNGNNNNNN NNNN
//
Primer: gene33-B_F1 (Pr0948)
Region: insert mid
Sequence file: RDB12227_B0Lld.seq check
>12227_12227_B0Ll_2_gene33-B_F1_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCTTANCTGT GCATCTGANC NGAACAGAGT TGTTCCAGAT
   61 CCGAACCCTC CCCCGCCTCA AAGCCATCGC AGATTAAGGA GGTCCCACTC AGGACCAGCC
  121 GGGTCGTTTA ACAAGCCAGC CATCCGGATA TCTAGCTGCA CACACAGAGC CTCTCCTAGC
  181 TCGGATGAAG ACAAGCCTGA GATCCCGCCC CGGGTTCCTA TACCTCCCAG GCCAGCCAAG
  241 CCAGACTACA GAAGGTGGTC AGCAGAAGTG ACCTCTAACA CATACAGTGA TGAAGATAGG
  301 CCTCCCAAAG TCCCCCCGAG AGAACCTTTG TCTCGGAGTA ACTCCCGTAC TCCAAGTCCT
  361 AAAAGCCTTC CGTCTTACCT CAATGGGGTC ATGCCTCCAA CACAGAGCTT TGCCCCCGAC
  421 CCCAAGTATG TCAGCAGCAA AGCCCCGCAG AGACAGAGCA GCGAAGGGTC TGCCAAGGCC
  481 CCCTGCATCC TGCCCATCAT TGAGAATGGG AAGAAGGTCA GCTCCACGCA TTATTACCTA
  541 CTACCTGAGA GACCGCCATA CCTTGACAAA TATGAGAAGT ATTTTAGGGA AGCAGAAGAA
  601 GCAAACCCAA GCACCCAGAT TCNGCCATTA CCTGCTGNCT GTGGTANGGN CTCTGNCACA
  661 GACAANCTGG NCTCCNNAAT GNAAATGGNN GTGGGCNGNN NCGGGNAGCN NAAACNCTTN
721 TCCTACNNGG TTNNNNNNNN NN
//
Primer: IRES-F (Pr0036)
Region: IRES, NeoR
Sequence file: RDB12227_B0Lle.seq check
>12227_12227_B0Ll_2_IRES-F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGTCN ANGTTNNNAA AACGTCTAGG CCCCCCGAAC CACGGGGNCG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAG CTTGCCACAA CCCGGGATAA TTCCTGCAGC
  121 CAATATGGGA TCGGCCATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
  181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
  241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
  301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
  361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
  421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
  481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
  541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
  601 NGANCTGGAC NAANANCATC ANNGGCTCGC GCCNGCCGAA CTGNTCGCCA GGCTCNAGGC
  661 GCGCATGCCC NANNNCGANG ATCTCNTCNN GNNCNNNGGN NNNGNCNGCN NNNNNAANNN
721 CATGGNNNNA AAANNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]