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SNAP-RALT

Mammalian expression clone of rat RALT.

Catalog number RDB12227
Resource name SNAP-RALT
Alternative name SNAP-tagged Gene-33
Clone info. Mammalian expression clone of rat RALT, SNAP-tagged. This clone contains a cDNA fragment corresponding to the 274 to 1649nt of X07266.1.
Comment Expression was observed by the depositor with the fluorescent detection using SNAP-tag substrate. Known differences (at least) : Substitutions with reference to X07266.1 are A to C 295nt (Gln to Pro), T to C 433nt (Leu to Pro), T to C 483nt (Ser to Pro), C to A 545nt (Pro, synonym), T to C 556nt (Ile to Thr), A to G 971nt (Pro, synonym), T to C 1013nt (Ser, synonym), C to T 1259nt (Thr, synonym), and T to C 1354nt (Leu to Pro).
Vector backbone pSNAP-tag(m) (Plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r
Gene/insert name Rat RALT cDNA
Depositor|Developer Takeda, Kenji |
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External Database
Rat RALT
          Reference sequence
            
           
          Sequence (full) RDB12227zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12227 SNAP-RALT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The SNAP-RALT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12227).

          Reference section:

          Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12227_B0Llp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SNAP_3_F (Pr0231)
          Region: SNAP-Tag, insert 5'
          Sequence file: RDB12227_B0Lla.seq check
          >12227_12227_B0Ll_SNAP_3_F_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGGGCA GCCTGGGCTG GGTCCTGCAG GTATAGGCGC
             61 GCCAGGAATG TCAACAGCAG GAGTTGCTGC TCCGGATATT CGAGTCCCAT TAAAAACTGG
            121 ATTTCTCCAT AATGGCCAGG CTTTGGGGAA TATGAAGACC TGCTGGGGCA GTCGCAATGA
            181 GTTTGAAAAG AATTTTTTAA ATATCGATCC AATAACCATG GCCTACAATC CGAACTCCCC
            241 TGCTCCGGAG CATCTAACAA CCCTCGGATG TGCTTCTCCG CCCGCTCCAG GGAGCGGTCA
            301 CTTCTTTGCA GAGCGTGGTC CATCTCCAAA GTCAAGCTTG CCACCTCTTG TTACCCCACC
            361 AAGTGAAAGC TCGGGACAGC GTGAAGAGGA TCAAGTTCTG TGTGGTTTTA AGAAACTCTC
            421 AGTGAATGGG GTGTGCGCTT CCACACCTCC ACTTACACCC ATTCAAAGCT GCTCTTCCCC
            481 GTTTCCCTGT GCAGCTCCCT GTGATCGCAG TTCCCGACCG CTCCCACCAT TGCCCATCTC
            541 TGANGACCCA TCTCTGGATG ANGCCGACTG TGANGTGGAA TTTCTAACCA GTGCAGATAC
            601 AGACTTCCTT TTANAAGANT GCGTGCCTTC CNATTTCNAA TACNATGTTC CTGGCANGCG
            661 AANCTTNCGT GGGTGCNGNN AGNNCAATTA NGCNNANTTN NNNNGCCNNA CNGNTNNNNN
          721 NNNNN
          //
          Primer: chimeric in-H_R (Pr0942)
          Region: insert 3'
          Sequence file: RDB12227_B0Llb.seq check
          >12227_12227_B0Ll_chimeric in-H_R_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNCNGG CCCTCGCAGA CAGCGAATTA ATTCCAGCAC ACTGGATCAG
             61 TTATCTATGC GGCCGCCTCA CTCGACTACG GAGAAACCAC GTAGGATAAG TGTTTGCGCT
            121 TCCCGTGGCC GCCCACATCC ATTTTCATTC TGGAGGCCAG CTTGTCTGTG GCAGAGACCA
            181 TACCACAGGC AGCAGGTAAT GGCTGAATCT GGGTGCTTGG GTTTGCTTCT TCTGCTTCCC
            241 TAAAATACTT CTCATATTTG TCAAGGTATG GCGGTCTCTC AGGTAGTAGG TAATAATGCG
            301 TGGAGCTGAC CTTCTTCCCA TTCTCAATGA TGGGCAGGAT GCAGGGGGCC TTGGCAGACC
            361 CTTCGCTGCT CTGTCTCTGC GGGGCTTTGC TGCTGACATA CTTGGGGTCG GGGGCAAAGC
            421 TCTGTGTTGG AGGCATGACC CCATTGAGGT AAGACGGAAG GCTTTTAGGA CTTGGAGTAC
            481 GGGAGTTACT CCGAGACAAA GGTTCTCTCG GGGGGACTTT GGGAGGCCTA TCTTCATCAC
            541 TGTATGTGTT AGAGGTCACT TCTGCTGACC ACCTTCTGTA GTCTGGCTTG NCTGGCCTGG
            601 GANGTATAGG AACCCGGGGC GGGNNCTCAN GCTTGTCTTC NTCCNAGCTA NGANANGCTC
            661 TGTGTGTGCA GCTAGATATC CNGNNGNNTG GCTTGNTNAA CNANCCNGCN NGNCCNGANN
          721 NGGNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, SNAP-Tag
          Sequence file: RDB12227_B0Llc.seq check
          >12227_12227_B0Ll_CMV_Forward_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNGC ANTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TTGGTACCGA GCTCGGATCG ATATCGAATT CACCATGGAC
            181 AAAGACTGCG AAATGAAGCG CACCACCCTG GATAGCCCTC TGGGCAAGCT GGAACTGTCT
            241 GGGTGCGAAC AGGGCCTGCA CGAGATCAAG CTGCTGGGCA AAGGAACATC TGCCGCCGAC
            301 GCCGTGGAAG TGCCTGCCCC AGCCGCCGTG CTGGGCGGAC CAGAGCCACT GATGCAGGCC
            361 ACCGCCTGGC TCAACGCCTA CTTTCACCAG CCTGAGGCCA TCGAGGAGTT CCCTGTGCCA
            421 GCCCTGCACC ACCCAGTGTT CCAGCAGGAG AGCTTTACCC GCCAGGTGCT GTGGAAACTG
            481 CTGAAAGTGG TGAAGTTCGG AGAGGTCATC AGCTACCAGC AGCTGGCCGC CCTGGCCGGC
            541 AATCCCGCCG CCACCGCCGC CGTGAAAACC GCCCTGANCG GAAATCCCGT GCCCATTCTG
            601 ATCCCCTGCC ACCGGGTGGT GTCTAGCTCT GGCGCCGTGG GGGGCTACNA NGGCGGGCTC
            661 GCCGTGAAAN ANTGGCTGCT GGNCNNCNNN GGNNACNGAN TGGGNNNNCN NNGNNTGGGT
          721 NNNGCNNNNN NNNGNNNNNN NNNN
          //
          Primer: gene33-B_F1 (Pr0948)
          Region: insert mid
          Sequence file: RDB12227_B0Lld.seq check
          >12227_12227_B0Ll_2_gene33-B_F1_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCTTANCTGT GCATCTGANC NGAACAGAGT TGTTCCAGAT
             61 CCGAACCCTC CCCCGCCTCA AAGCCATCGC AGATTAAGGA GGTCCCACTC AGGACCAGCC
            121 GGGTCGTTTA ACAAGCCAGC CATCCGGATA TCTAGCTGCA CACACAGAGC CTCTCCTAGC
            181 TCGGATGAAG ACAAGCCTGA GATCCCGCCC CGGGTTCCTA TACCTCCCAG GCCAGCCAAG
            241 CCAGACTACA GAAGGTGGTC AGCAGAAGTG ACCTCTAACA CATACAGTGA TGAAGATAGG
            301 CCTCCCAAAG TCCCCCCGAG AGAACCTTTG TCTCGGAGTA ACTCCCGTAC TCCAAGTCCT
            361 AAAAGCCTTC CGTCTTACCT CAATGGGGTC ATGCCTCCAA CACAGAGCTT TGCCCCCGAC
            421 CCCAAGTATG TCAGCAGCAA AGCCCCGCAG AGACAGAGCA GCGAAGGGTC TGCCAAGGCC
            481 CCCTGCATCC TGCCCATCAT TGAGAATGGG AAGAAGGTCA GCTCCACGCA TTATTACCTA
            541 CTACCTGAGA GACCGCCATA CCTTGACAAA TATGAGAAGT ATTTTAGGGA AGCAGAAGAA
            601 GCAAACCCAA GCACCCAGAT TCNGCCATTA CCTGCTGNCT GTGGTANGGN CTCTGNCACA
            661 GACAANCTGG NCTCCNNAAT GNAAATGGNN GTGGGCNGNN NCGGGNAGCN NAAACNCTTN
          721 TCCTACNNGG TTNNNNNNNN NN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES, NeoR
          Sequence file: RDB12227_B0Lle.seq check
          >12227_12227_B0Ll_2_IRES-F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNGTCN ANGTTNNNAA AACGTCTAGG CCCCCCGAAC CACGGGGNCG
             61 TGGTTTTCCT TTGAAAAACA CGATGATAAG CTTGCCACAA CCCGGGATAA TTCCTGCAGC
            121 CAATATGGGA TCGGCCATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
            181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
            241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
            301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
            361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
            421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
            481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
            541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
            601 NGANCTGGAC NAANANCATC ANNGGCTCGC GCCNGCCGAA CTGNTCGCCA GGCTCNAGGC
            661 GCGCATGCCC NANNNCGANG ATCTCNTCNN GNNCNNNGGN NNNGNCNGCN NNNNNAANNN
          721 CATGGNNNNA AAANNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl