Please review the QC test results indicated by icon below as well as clone information before placing your order.

His-Xpress-RALT S250A mutant

Mammalian expression clone of rat RALT, S250A mutant

Sequence (full)	RDB12226zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12226_B1Bcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, T7 pro, QBI SP163, 6xHis, Xpress tag, insert 5' Sequence file: RDB12226_B1Bca.seq
>D01392A2_B1Bc_2_CMV-Forward_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNCNNTTG NNGCNNNNGG GCGGTANGCG TGTACGGTGG GANGTCTATA    61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA   121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCGTTTAAA CTTAAGCTTA GCGCAGAGGC   181 TTGGGGCAGC CGAGCGGCAG CCAGGCCCCG GCCCGGGCCT CGGTTCCAGA AGGGAGAGGA   241 GCCCGCCAAG GCGCGCAAGA GAGCGGGCTG CCTCGCAGTC CGAGCCGGAG AGGGAGCGCG   301 AGCCGCGCCG GCCCCGGACG GCCTCCGAAA CCATGGGGGG TTCTCATCAT CATCATCATC   361 ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG   421 ATAAGGTACA GGCCCTTGGT TCAACAGCAG GAGTTGCTGC TCCGGATATT CGAGTCCCAT   481 TAAAAACTGG ATTTCTCCAT AATGGCCAGG CTTTGGGGAA TATGAAGACC TGCTGGGGCA   541 GTCGCAATGA GTTTGAAAAG AATTTTTTAA ATATCGATCC AATAACCATG GCCTACAATC   601 CGAACTCCCC TGCTCCGGAG CATCTAACAA CCCTCGGATG TGCTTCTCCG CCAGCTCCAG   661 GGAGCGGTCA CTTCTTTGCA GAGCGTGGTC CATCTCCAAA GTCAAGCTTG CCACCTCTTG   721 TTACCCCACC AAGTGAAAGC TCGGGACAGC GTGAAGAGGN TCAAGTTCTG TGTGGTTTTA   781 AGAAACTCTC AGTGAATGGG GTGTGCGCTT CCACACCTCC ACTTACACCC ATTCNAAGCT   841 GCTCTTCCCC GTTTCCCNGT GCAGCTCCCT GTGATCGCAG TTCCCGACCG CNCCCACCAT   901 TGCCCATCTC TGANGNNCNN TCTCTGNNNG ANGCCNANNG NNANGNGGAA NTTCTAACCN   961 GNGCNNANAC NNNNTNNCTT TTANNNNNNN GNNNGNCNNN NNNNNTTNNA NNNNNANNNN 1021 NCNNNNNNNN NN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, insert 3' Sequence file: RDB12226_B1Bcb.seq
>D01392A2_B1Bc_2_BGH_rev2_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GGGNNGGGNA NNAACAGNTG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CCACTGTGCT   121 GGATATCTGC AGAATTCCAC CACACTGGAT CCTAGGTACA GGCCCTTCTA CGGAGAAACC   181 ACGTAGGATA AGTGTTTGCG CTTCCCGTGG CCGCCCACAT CCATTTTCAT TCTGGAGGCC   241 AGCTTGTCTG TGGCAGAGAC CATACCACAG GCAGCAGGTA ATGGCTGAAT CTGGGTGCTT   301 GGGTTTGCTT CTTCTGCTTC CCTAAAATAC TTCTCATATT TGTCAAGGTA TGGCGGTCTC   361 TCAGGTAGTA GGTAATAATG CGTGGAGCTG ACCTTCTTCC CATTCTCAAT GATGGGCAGG   421 ATGCAGGGGG CCTTGGCAGA CCCTTCGCTG CTCTGTCTCT GCGGGGCTTT GCTGCTGACA   481 TACTTGGGGT CGGGGGCAAA GCTCTGTGTT GGAGGCATGA CCCCATTGAG GTAAGACGGA   541 AGGCTTTTAG GACTTGGAGT ACGGGAGTTA CTCCGAGACA AAGGTTCTCT CGGGGGGACT   601 TTGGGAGGCC TATCTTCATC ACTGTATGTG TTAGAGGTCA CTTCTGCTGA CCACCTTCTG   661 TAGTCTGGCT TGGCTGGCCT GGGAGGTATA GGAACCCGGG GCGGGATCTC AGGCTTGTCT   721 TCATCCGAGC TANGANAGGC TCTGTGTGTG CAGCTAGATA TCCGGATGGC TGGCTTGTTA   781 AACGACCCGG CTGGTCCTGC GTGGGACCTC CTTAATCTGC GATGGCTTTG ANGCGGGGGA   841 NGNNTCGGAT CTGGAACAAC TCTGTTCTGG TCANATGCAC AGCTAAGATC TGNNNCAGAA   901 ACANTTGGGC TGNCNAAATA NNCATAATTG NTCTGNNCNC ANCCNCNGAA GCTTCNCCNG 961 NNNNGNANNN TCNNANTTGA AANNNGNNNN NNNNNN //
Primer: gene33-M_F1 (Pr0947) Region: insert mid (S250 WT) Sequence file: RDB12226_B1Bcc.seq
>D01392A2_B1Bc_2_gene33-M_F1_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN ANNNCTTAGC TGTGCNTCTG ANCAGAACAG AGTTGTTCCN    61 GATCCGAACC CTCCCCCGCC TCAAAGCCAT CGCAGATTAA GGAGGTCCCA CGCAGGACCA   121 GCCGGGTCGT TTAACAAGCC AGCCATCCGG ATATCTAGCT GCACACACAG AGCCTCTCCT   181 AGCTCGGATG AAGACAAGCC TGAGATCCCG CCCCGGGTTC CTATACCTCC CAGGCCAGCC   241 AAGCCAGACT ACAGAAGGTG GTCAGCAGAA GTGACCTCTA ACACATACAG TGATGAAGAT   301 AGGCCTCCCA AAGTCCCCCC GAGAGAACCT TTGTCTCGGA GTAACTCCCG TACTCCAAGT   361 CCTAAAAGCC TTCCGTCTTA CCTCAATGGG GTCATGCCTC CAACACAGAG CTTTGCCCCC   421 GACCCCAAGT ATGTCAGCAG CAAAGCCCCG CAGAGACAGA GCAGCGAAGG GTCTGCCAAG   481 GCCCCCTGCA TCCTGCCCAT CATTGAGAAT GGGAAGAAGG TCAGCTCCAC GCATTATTAC   541 CTACTACCTG AGAGACCGCC ATACCTTGAC AAATATGAGA AGTATTTTAG GGAAGCAGAA   601 GAAGCAAACC CAAGCACCCA GATTCAGCCA TTACCTGCTG CCTGTGGTAT GGTCTCTGCC   661 ACAGACAAGC TGGCCTCCAG AATGAAAATG GATGTGGGCG GCCACGGGAA GCGCAAACAC   721 TTATCCTACG TGGTTTCTCC GTAGAANGGC CTGTACCTAG GNTCCAGTGT GGTGGAATTC   781 TGCAGATATC CAGCACAGTG GCGGCCGCTC GANTCTAGAN GGCCCGTTTA AACCCGCTGA   841 TCAGCCTCGA CTGTGCCTTC TANTTGNCAG CCATCTGTTG TTTGCCCCTC CCCCGNGCCN   901 NNCNTGANCC NGGANNNNGN CACTCCCACN GNCCTTTNCN AANAAANNGN NGNAAATTGN   961 NTCGCATTGN CTGANNNNNN NGNCNTNNNN NNNGGGGGNN NNGGNNNNNG NNNNNANNNN 1021 NNNGNGNNNN NNNNNGNNNN NNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, EM7 pro, BleoR, SV40 pA Sequence file: RDB12226_B1Bcd.seq
>D01392A2_B1Bc_2_SV40pro_ori_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NGCCTCTGCC TCTGNNCTAT TCCNGAAGTA GTGAGGAGGC    61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA   121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG   181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC   241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG   301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC   361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC   421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA   481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA   541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA   601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA   661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG   721 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT   781 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC   841 CGTCNANCTC TAGCTAGAGC TTGGCGTANT CATGGTCATA GCTGNTTCCT GTGTGAAATT   901 GNTATCCGCT CACAATTCCN CACAACATAC NANNNNGAAN CATAAANNGT AAAGCCNGGG 961 GNGNCNANNG ANNGANCTNN NNNANNTTNN NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl