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His-Xpress-RALT S250A mutant (#RDB12226)

Mammalian expression clone of rat RALT, S250A mutant

Alternative name Gene-33 S250A mutant
Clone info. Mammalian expression clone of rat RALT, S250A mutant.
Comment Expression was observed by the depositor the Western blotting using anti-Xpress-tag, anti-His-tag and anti-HisG-tag antibody.
Vector backbone pcDNA4/HisMax-TOPO (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Rat RALT cDNA
Depositor|Developer Takeda, Kenji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12226 His-Xpress-RALT S250A mutant DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The His-Xpress-RALT S250A mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12226).

Reference section:

Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12226_B1Bcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, T7 pro, QBI SP163, 6xHis, Xpress tag, insert 5'
Sequence file: RDB12226_B1Bca.seq check
>D01392A2_B1Bc_2_CMV-Forward_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNTTG NNGCNNNNGG GCGGTANGCG TGTACGGTGG GANGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCGTTTAAA CTTAAGCTTA GCGCAGAGGC
  181 TTGGGGCAGC CGAGCGGCAG CCAGGCCCCG GCCCGGGCCT CGGTTCCAGA AGGGAGAGGA
  241 GCCCGCCAAG GCGCGCAAGA GAGCGGGCTG CCTCGCAGTC CGAGCCGGAG AGGGAGCGCG
  301 AGCCGCGCCG GCCCCGGACG GCCTCCGAAA CCATGGGGGG TTCTCATCAT CATCATCATC
  361 ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG
  421 ATAAGGTACA GGCCCTTGGT TCAACAGCAG GAGTTGCTGC TCCGGATATT CGAGTCCCAT
  481 TAAAAACTGG ATTTCTCCAT AATGGCCAGG CTTTGGGGAA TATGAAGACC TGCTGGGGCA
  541 GTCGCAATGA GTTTGAAAAG AATTTTTTAA ATATCGATCC AATAACCATG GCCTACAATC
  601 CGAACTCCCC TGCTCCGGAG CATCTAACAA CCCTCGGATG TGCTTCTCCG CCAGCTCCAG
  661 GGAGCGGTCA CTTCTTTGCA GAGCGTGGTC CATCTCCAAA GTCAAGCTTG CCACCTCTTG
  721 TTACCCCACC AAGTGAAAGC TCGGGACAGC GTGAAGAGGN TCAAGTTCTG TGTGGTTTTA
  781 AGAAACTCTC AGTGAATGGG GTGTGCGCTT CCACACCTCC ACTTACACCC ATTCNAAGCT
  841 GCTCTTCCCC GTTTCCCNGT GCAGCTCCCT GTGATCGCAG TTCCCGACCG CNCCCACCAT
  901 TGCCCATCTC TGANGNNCNN TCTCTGNNNG ANGCCNANNG NNANGNGGAA NTTCTAACCN
  961 GNGCNNANAC NNNNTNNCTT TTANNNNNNN GNNNGNCNNN NNNNNTTNNA NNNNNANNNN
1021 NCNNNNNNNN NN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA, insert 3'
Sequence file: RDB12226_B1Bcb.seq check
>D01392A2_B1Bc_2_BGH_rev2_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GGGNNGGGNA NNAACAGNTG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CCACTGTGCT
  121 GGATATCTGC AGAATTCCAC CACACTGGAT CCTAGGTACA GGCCCTTCTA CGGAGAAACC
  181 ACGTAGGATA AGTGTTTGCG CTTCCCGTGG CCGCCCACAT CCATTTTCAT TCTGGAGGCC
  241 AGCTTGTCTG TGGCAGAGAC CATACCACAG GCAGCAGGTA ATGGCTGAAT CTGGGTGCTT
  301 GGGTTTGCTT CTTCTGCTTC CCTAAAATAC TTCTCATATT TGTCAAGGTA TGGCGGTCTC
  361 TCAGGTAGTA GGTAATAATG CGTGGAGCTG ACCTTCTTCC CATTCTCAAT GATGGGCAGG
  421 ATGCAGGGGG CCTTGGCAGA CCCTTCGCTG CTCTGTCTCT GCGGGGCTTT GCTGCTGACA
  481 TACTTGGGGT CGGGGGCAAA GCTCTGTGTT GGAGGCATGA CCCCATTGAG GTAAGACGGA
  541 AGGCTTTTAG GACTTGGAGT ACGGGAGTTA CTCCGAGACA AAGGTTCTCT CGGGGGGACT
  601 TTGGGAGGCC TATCTTCATC ACTGTATGTG TTAGAGGTCA CTTCTGCTGA CCACCTTCTG
  661 TAGTCTGGCT TGGCTGGCCT GGGAGGTATA GGAACCCGGG GCGGGATCTC AGGCTTGTCT
  721 TCATCCGAGC TANGANAGGC TCTGTGTGTG CAGCTAGATA TCCGGATGGC TGGCTTGTTA
  781 AACGACCCGG CTGGTCCTGC GTGGGACCTC CTTAATCTGC GATGGCTTTG ANGCGGGGGA
  841 NGNNTCGGAT CTGGAACAAC TCTGTTCTGG TCANATGCAC AGCTAAGATC TGNNNCAGAA
  901 ACANTTGGGC TGNCNAAATA NNCATAATTG NTCTGNNCNC ANCCNCNGAA GCTTCNCCNG
961 NNNNGNANNN TCNNANTTGA AANNNGNNNN NNNNNN
//
Primer: gene33-M_F1 (Pr0947)
Region: insert mid (S250 WT)
Sequence file: RDB12226_B1Bcc.seq check
>D01392A2_B1Bc_2_gene33-M_F1_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN ANNNCTTAGC TGTGCNTCTG ANCAGAACAG AGTTGTTCCN
   61 GATCCGAACC CTCCCCCGCC TCAAAGCCAT CGCAGATTAA GGAGGTCCCA CGCAGGACCA
  121 GCCGGGTCGT TTAACAAGCC AGCCATCCGG ATATCTAGCT GCACACACAG AGCCTCTCCT
  181 AGCTCGGATG AAGACAAGCC TGAGATCCCG CCCCGGGTTC CTATACCTCC CAGGCCAGCC
  241 AAGCCAGACT ACAGAAGGTG GTCAGCAGAA GTGACCTCTA ACACATACAG TGATGAAGAT
  301 AGGCCTCCCA AAGTCCCCCC GAGAGAACCT TTGTCTCGGA GTAACTCCCG TACTCCAAGT
  361 CCTAAAAGCC TTCCGTCTTA CCTCAATGGG GTCATGCCTC CAACACAGAG CTTTGCCCCC
  421 GACCCCAAGT ATGTCAGCAG CAAAGCCCCG CAGAGACAGA GCAGCGAAGG GTCTGCCAAG
  481 GCCCCCTGCA TCCTGCCCAT CATTGAGAAT GGGAAGAAGG TCAGCTCCAC GCATTATTAC
  541 CTACTACCTG AGAGACCGCC ATACCTTGAC AAATATGAGA AGTATTTTAG GGAAGCAGAA
  601 GAAGCAAACC CAAGCACCCA GATTCAGCCA TTACCTGCTG CCTGTGGTAT GGTCTCTGCC
  661 ACAGACAAGC TGGCCTCCAG AATGAAAATG GATGTGGGCG GCCACGGGAA GCGCAAACAC
  721 TTATCCTACG TGGTTTCTCC GTAGAANGGC CTGTACCTAG GNTCCAGTGT GGTGGAATTC
  781 TGCAGATATC CAGCACAGTG GCGGCCGCTC GANTCTAGAN GGCCCGTTTA AACCCGCTGA
  841 TCAGCCTCGA CTGTGCCTTC TANTTGNCAG CCATCTGTTG TTTGCCCCTC CCCCGNGCCN
  901 NNCNTGANCC NGGANNNNGN CACTCCCACN GNCCTTTNCN AANAAANNGN NGNAAATTGN
  961 NTCGCATTGN CTGANNNNNN NGNCNTNNNN NNNGGGGGNN NNGGNNNNNG NNNNNANNNN
1021 NNNGNGNNNN NNNNNGNNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, EM7 pro, BleoR, SV40 pA
Sequence file: RDB12226_B1Bcd.seq check
>D01392A2_B1Bc_2_SV40pro_ori_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NGCCTCTGCC TCTGNNCTAT TCCNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
  121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG
  181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
  241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
  301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
  361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
  421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
  481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
  541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA
  601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA
  661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG
  721 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  781 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC
  841 CGTCNANCTC TAGCTAGAGC TTGGCGTANT CATGGTCATA GCTGNTTCCT GTGTGAAATT
  901 GNTATCCGCT CACAATTCCN CACAACATAC NANNNNGAAN CATAAANNGT AAAGCCNGGG
961 GNGNCNANNG ANNGANCTNN NNNANNTTNN NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]