Mammalian expression clone of rat RALT, S250A mutant
Alternative name | Gene-33 S250A mutant |
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Clone info. | Mammalian expression clone of rat RALT, S250A mutant. |
Comment | Expression was observed by the depositor the Western blotting using anti-Xpress-tag, anti-His-tag and anti-HisG-tag antibody. |
Vector backbone | pcDNA4/HisMax-TOPO (Plasmid) |
Size of vector backbone | 5.2 kb |
Selectable markers | Amp^r |
Gene/insert name | Rat RALT cDNA |
Depositor|Developer | Takeda, Kenji | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB12226 | His-Xpress-RALT S250A mutant | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The His-Xpress-RALT S250A mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12226). |
Reference section:
Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12226_B1Bcp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro, T7 pro, QBI SP163, 6xHis, Xpress tag, insert 5' Sequence file: RDB12226_B1Bca.seq ![]() |
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>D01392A2_B1Bc_2_CMV-Forward_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNCNNTTG NNGCNNNNGG GCGGTANGCG TGTACGGTGG GANGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCGTTTAAA CTTAAGCTTA GCGCAGAGGC 181 TTGGGGCAGC CGAGCGGCAG CCAGGCCCCG GCCCGGGCCT CGGTTCCAGA AGGGAGAGGA 241 GCCCGCCAAG GCGCGCAAGA GAGCGGGCTG CCTCGCAGTC CGAGCCGGAG AGGGAGCGCG 301 AGCCGCGCCG GCCCCGGACG GCCTCCGAAA CCATGGGGGG TTCTCATCAT CATCATCATC 361 ATGGTATGGC TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG 421 ATAAGGTACA GGCCCTTGGT TCAACAGCAG GAGTTGCTGC TCCGGATATT CGAGTCCCAT 481 TAAAAACTGG ATTTCTCCAT AATGGCCAGG CTTTGGGGAA TATGAAGACC TGCTGGGGCA 541 GTCGCAATGA GTTTGAAAAG AATTTTTTAA ATATCGATCC AATAACCATG GCCTACAATC 601 CGAACTCCCC TGCTCCGGAG CATCTAACAA CCCTCGGATG TGCTTCTCCG CCAGCTCCAG 661 GGAGCGGTCA CTTCTTTGCA GAGCGTGGTC CATCTCCAAA GTCAAGCTTG CCACCTCTTG 721 TTACCCCACC AAGTGAAAGC TCGGGACAGC GTGAAGAGGN TCAAGTTCTG TGTGGTTTTA 781 AGAAACTCTC AGTGAATGGG GTGTGCGCTT CCACACCTCC ACTTACACCC ATTCNAAGCT 841 GCTCTTCCCC GTTTCCCNGT GCAGCTCCCT GTGATCGCAG TTCCCGACCG CNCCCACCAT 901 TGCCCATCTC TGANGNNCNN TCTCTGNNNG ANGCCNANNG NNANGNGGAA NTTCTAACCN 961 GNGCNNANAC NNNNTNNCTT TTANNNNNNN GNNNGNCNNN NNNNNTTNNA NNNNNANNNN 1021 NCNNNNNNNN NN // |
Primer: BGH_rev2 (Pr0606) Region: bGH pA, insert 3' Sequence file: RDB12226_B1Bcb.seq ![]() |
>D01392A2_B1Bc_2_BGH_rev2_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GGGNNGGGNA NNAACAGNTG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CCACTGTGCT 121 GGATATCTGC AGAATTCCAC CACACTGGAT CCTAGGTACA GGCCCTTCTA CGGAGAAACC 181 ACGTAGGATA AGTGTTTGCG CTTCCCGTGG CCGCCCACAT CCATTTTCAT TCTGGAGGCC 241 AGCTTGTCTG TGGCAGAGAC CATACCACAG GCAGCAGGTA ATGGCTGAAT CTGGGTGCTT 301 GGGTTTGCTT CTTCTGCTTC CCTAAAATAC TTCTCATATT TGTCAAGGTA TGGCGGTCTC 361 TCAGGTAGTA GGTAATAATG CGTGGAGCTG ACCTTCTTCC CATTCTCAAT GATGGGCAGG 421 ATGCAGGGGG CCTTGGCAGA CCCTTCGCTG CTCTGTCTCT GCGGGGCTTT GCTGCTGACA 481 TACTTGGGGT CGGGGGCAAA GCTCTGTGTT GGAGGCATGA CCCCATTGAG GTAAGACGGA 541 AGGCTTTTAG GACTTGGAGT ACGGGAGTTA CTCCGAGACA AAGGTTCTCT CGGGGGGACT 601 TTGGGAGGCC TATCTTCATC ACTGTATGTG TTAGAGGTCA CTTCTGCTGA CCACCTTCTG 661 TAGTCTGGCT TGGCTGGCCT GGGAGGTATA GGAACCCGGG GCGGGATCTC AGGCTTGTCT 721 TCATCCGAGC TANGANAGGC TCTGTGTGTG CAGCTAGATA TCCGGATGGC TGGCTTGTTA 781 AACGACCCGG CTGGTCCTGC GTGGGACCTC CTTAATCTGC GATGGCTTTG ANGCGGGGGA 841 NGNNTCGGAT CTGGAACAAC TCTGTTCTGG TCANATGCAC AGCTAAGATC TGNNNCAGAA 901 ACANTTGGGC TGNCNAAATA NNCATAATTG NTCTGNNCNC ANCCNCNGAA GCTTCNCCNG 961 NNNNGNANNN TCNNANTTGA AANNNGNNNN NNNNNN // |
Primer: gene33-M_F1 (Pr0947) Region: insert mid (S250 WT) Sequence file: RDB12226_B1Bcc.seq ![]() |
>D01392A2_B1Bc_2_gene33-M_F1_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN ANNNCTTAGC TGTGCNTCTG ANCAGAACAG AGTTGTTCCN 61 GATCCGAACC CTCCCCCGCC TCAAAGCCAT CGCAGATTAA GGAGGTCCCA CGCAGGACCA 121 GCCGGGTCGT TTAACAAGCC AGCCATCCGG ATATCTAGCT GCACACACAG AGCCTCTCCT 181 AGCTCGGATG AAGACAAGCC TGAGATCCCG CCCCGGGTTC CTATACCTCC CAGGCCAGCC 241 AAGCCAGACT ACAGAAGGTG GTCAGCAGAA GTGACCTCTA ACACATACAG TGATGAAGAT 301 AGGCCTCCCA AAGTCCCCCC GAGAGAACCT TTGTCTCGGA GTAACTCCCG TACTCCAAGT 361 CCTAAAAGCC TTCCGTCTTA CCTCAATGGG GTCATGCCTC CAACACAGAG CTTTGCCCCC 421 GACCCCAAGT ATGTCAGCAG CAAAGCCCCG CAGAGACAGA GCAGCGAAGG GTCTGCCAAG 481 GCCCCCTGCA TCCTGCCCAT CATTGAGAAT GGGAAGAAGG TCAGCTCCAC GCATTATTAC 541 CTACTACCTG AGAGACCGCC ATACCTTGAC AAATATGAGA AGTATTTTAG GGAAGCAGAA 601 GAAGCAAACC CAAGCACCCA GATTCAGCCA TTACCTGCTG CCTGTGGTAT GGTCTCTGCC 661 ACAGACAAGC TGGCCTCCAG AATGAAAATG GATGTGGGCG GCCACGGGAA GCGCAAACAC 721 TTATCCTACG TGGTTTCTCC GTAGAANGGC CTGTACCTAG GNTCCAGTGT GGTGGAATTC 781 TGCAGATATC CAGCACAGTG GCGGCCGCTC GANTCTAGAN GGCCCGTTTA AACCCGCTGA 841 TCAGCCTCGA CTGTGCCTTC TANTTGNCAG CCATCTGTTG TTTGCCCCTC CCCCGNGCCN 901 NNCNTGANCC NGGANNNNGN CACTCCCACN GNCCTTTNCN AANAAANNGN NGNAAATTGN 961 NTCGCATTGN CTGANNNNNN NGNCNTNNNN NNNGGGGGNN NNGGNNNNNG NNNNNANNNN 1021 NNNGNGNNNN NNNNNGNNNN NNNNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, EM7 pro, BleoR, SV40 pA Sequence file: RDB12226_B1Bcd.seq ![]() |
>D01392A2_B1Bc_2_SV40pro_ori_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NGCCTCTGCC TCTGNNCTAT TCCNGAAGTA GTGAGGAGGC 61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG 181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC 241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG 301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC 361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC 421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA 481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA 541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA 601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 721 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 781 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 841 CGTCNANCTC TAGCTAGAGC TTGGCGTANT CATGGTCATA GCTGNTTCCT GTGTGAAATT 901 GNTATCCGCT CACAATTCCN CACAACATAC NANNNNGAAN CATAAANNGT AAAGCCNGGG 961 GNGNCNANNG ANNGANCTNN NNNANNTTNN NTNNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP] |
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