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His-Xpress-RALT

Mammalian expression clone of rat RALT

Catalog number RDB12225
Resource name His-Xpress-RALT
Alternative name Gene-33
Clone info. Mammalian expression clone of rat RALT.
Comment Expression was observed by the depositor with the Western blotting using anti-Xpress-tag, anti-His-tag and anti-HisG-tag antibody.
Vector backbone pcDNA4/HisMax-TOPO (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Rat RALT cDNA
Depositor|Developer Takeda, Kenji |
Other clones in our bank
External Database
Rat RALT
          Reference sequence
            
           
          Sequence (full) RDB12225zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB12225 His-Xpress-RALT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The His-Xpress-RALT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12225).

          Reference section:

          Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12225_B1Bcp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, T7 pro, QBI SP163, 6xHis, Xpress tag, insert 5'
          Sequence file: RDB12225_B1Bca.seq check
          >D01392A1_B1Bc_3_CMV-Forward_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNGCNNNGGG CGGTAGGCGT GTACGGTGGG NGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTAG CGCAGAGGCT
            181 TGGGGCAGCC GAGCGGCAGC CAGGCCCCGG CCCGGGCCTC GGTTCCAGAA GGGAGAGGAG
            241 CCCGCCAAGG CGCGCAAGAG AGCGGGCTGC CTCGCAGTCC GAGCCGGAGA GGGAGCGCGA
            301 GCCGCGCCGG CCCCGGACGG CCTCCGAAAC CATGGGGGGT TCTCATCATC ATCATCATCA
            361 TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA
            421 TAAGGTACAG GCCCTTGGTT CAACAGCAGG AGTTGCTGCT CCGGATATTC GAGTCCCATT
            481 AAAAACTGGA TTTCTCCATA ATGGCCAGGC TTTGGGGAAT ATGAAGACCT GCTGGGGCAG
            541 TCGCAATGAG TTTGAAAAGA ATTTTTTAAA TATCGATCCA ATAACCATGG CCTACAATCC
            601 GAACTCCCCT GCTCCGGAGC ATCTAACAAC CCTCGGATGT GCTTCTCCGC CCGCTCCAGG
            661 GAGCGGTCAC TTCTTTGCAG AGCGTGGTCC ATCTCCAAAG TCAAGCTTGC CACCTCTTGT
            721 TACCCCACCA AGTGAAAGCT CGGGACAGCG TGAAGAGGAT CAAGTTCTGT GTGGTTTTAA
            781 GAAACTCTCA GTGAATGGGG TGTGCGCTTC CACACCTCCA CTTACACCCA TTCAAAGCTG
            841 CTCTTCCCCG TTTCCCTGTG CAGCTCCCTG NGATCGCAGT TCCCGANCGC TCCCACCATT
            901 GCCCATCTCT GANGANCCNT CTCNGNNTGA NGNCNANTGN GNNNNNNNAN TTTCNNANNN
          961 NNGNANANAC NNNNNNNCTT TTNNNANNNN NGNNNGNCNN NNNNNNTNNN NNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA, insert 3'
          Sequence file: RDB12225_B1Bcb.seq check
          >D01392A1_B1Bc_3_BGH_rev2_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGG GNNGGGCANC AACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG
            121 ATATCTGCAG AATTCCACCA CACTGGATCC TAGGTACAGG CCCTTCTACG GAGAAACCAC
            181 GTAGGATAAG TGTTTGCGCT TCCCGTGGCC GCCCACATCC ATTTTCATTC TGGAGGCCAG
            241 CTTGTCTGTG GCAGAGACCA TACCACAGGC AGCAGGTAAT GGCTGAATCT GGGTGCTTGG
            301 GTTTGCTTCT TCTGCTTCCC TAAAATACTT CTCATATTTG TCAAGGTATG GCGGTCTCTC
            361 AGGTAGTAGG TAATAATGCG TGGAGCTGAC CTTCTTCCCA TTCTCAATGA TGGGCAGGAT
            421 GCAGGGGGCC TTGGCAGACC CTTCGCTGCT CTGTCTCTGC GGGGCTTTGC TGCTGACATA
            481 CTTGGGGTCG GGGGCAAAGC TCTGTGTTGG AGGCATGACC CCATTGAGGT AAGACGGAAG
            541 GCTTTTAGGA CTTGGAGTAC GGGAGTTACT CCGAGACAAA GGTTCTCTCG GGGGGACTTT
            601 GGGAGGCCTA TCTTCATCAC TGTATGTGTT AGAGGTCACT TCTGCTGACC ACCTTCTGTA
            661 GTCTGGCTTG GCTGGCCTGG GAGGTATAGG AACCCGGGGC GGGATCTCAG GCTTGTCTTC
            721 ATCCGAGCTA GGAGAGGCTC TGTGTGTGCA GCTAGATATC CGGATGGCTG GCTTGTTAAA
            781 CGACCCGGCT GGTCCTGANT GGGACCTCCT TAATCTGCGA TGGCTTTGAN GCGGGGGANG
            841 GTTCGGATCT GGAACAACTC TGTTCTGGTC ANATGCACAG CTAANNTCTG NCACAGAAAC
            901 AGTTGGNCTG NCAAAATACG CATAATTGNT CTGNCCNCAN CCNCNGNAGC TTCNNCNGNN
          961 NNNNNNNTCN NATTNNNANT CGGNANNNNN NNNNN
          //
          Primer: gene33-M_F1 (Pr0947)
          Region: insert mid (S250 WT)
          Sequence file: RDB12225_B1Bcc.seq check
          >D01392A1_B1Bc_3_gene33-M_F1_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCTTANC TGTGNNNNTG ANCNGAACAG AGTTGTTCCN
             61 GATCCGAACC CTCCCCCGCC TCAAAGCCAT CGCAGATTAA GGAGGTCCCA CTCAGGACCA
            121 GCCGGGTCGT TTAACAAGCC AGCCATCCGG ATATCTAGCT GCACACACAG AGCCTCTCCT
            181 AGCTCGGATG AAGACAAGCC TGAGATCCCG CCCCGGGTTC CTATACCTCC CAGGCCAGCC
            241 AAGCCAGACT ACAGAAGGTG GTCAGCAGAA GTGACCTCTA ACACATACAG TGATGAAGAT
            301 AGGCCTCCCA AAGTCCCCCC GAGAGAACCT TTGTCTCGGA GTAACTCCCG TACTCCAAGT
            361 CCTAAAAGCC TTCCGTCTTA CCTCAATGGG GTCATGCCTC CAACACAGAG CTTTGCCCCC
            421 GACCCCAAGT ATGTCAGCAG CAAAGCCCCG CAGAGACAGA GCAGCGAAGG GTCTGCCAAG
            481 GCCCCCTGCA TCCTGCCCAT CATTGAGAAT GGGAAGAAGG TCAGCTCCAC GCATTATTAC
            541 CTACTACCTG AGAGACCGCC ATACCTTGAC AAATATGAGA AGTATTTTAG GGAAGCAGAA
            601 GAAGCAAACC CAAGCACCCA GATTCAGCCA TTACCTGCTG CCTGTGGTAT GGTCTCTGCC
            661 ACAGACAAGC TGGCCTCCAG AATGAAAATG GATGTGGGCG GCCACGGGAA GCGCAAACAC
            721 TTATCCTACG TGGTTTCTCC GTANAAGGGC CTGTACCTAG GATCCAGTGT GGTGGAATTC
            781 TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGTCTAGAG GGCCCGTTTA AACCCGCTGA
            841 TCAGCCTCNA CTGTGCCTTC TANTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGNCT
            901 NNNNTGACCN NGNANNGNGC CANTCCNNCT NNCCTTTNNN NNNAAANTGA GNAAATTGCA
            961 TCGCANTNGN CNGANTNNNT GTCNNTCTAT NNNNGGGGGN NNGGGNNNGG GNNNNNNNAN
          1021 NNANNGGGGG NNNGNNNNNN NNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, EM7 pro, BleoR, SV40 pA
          Sequence file: RDB12225_B1Bcd.seq check
          >D01392A1_B1Bc_3_SV40pro_ori_F_D01_10_ABI24.ab1
              1 NNNNNNNNNN NGNNNNNNNN NNNNCTCTGC CTCTGNNCTA TTCCNGAAGT AGTGAGGAGG
             61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
            121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC
            181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG
            241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC
            301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC
            361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG
            421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC
            481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC
            541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC
            601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
            661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
            721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
            781 TCACTGCATT CTANTTGTGN TTTGTCNAAA CTCATCAATG TATCTTATCA TGTCTGTATA
            841 CCGTCNANCT CTANCTAGAG CTTGGCGTAN TCATGGNCAT AGCTGTTNCC TGNNNGAAAT
            901 TGTTATNNGC TCACNATTCC NCACNACATA CNAANCCNNN ANNATAAAGT GTAAAGCCTG
          961 GGGGNGNCNA ANGAGTGANC TNACNNNCAT TNANNNNCNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl