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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

His-Xpress-RALT (#RDB12225)

Mammalian expression clone of rat RALT

Alternative name Gene-33
Clone info. Mammalian expression clone of rat RALT.
Comment Expression was observed by the depositor with the Western blotting using anti-Xpress-tag, anti-His-tag and anti-HisG-tag antibody.
Vector backbone pcDNA4/HisMax-TOPO (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Rat RALT cDNA
Depositor|Developer Takeda, Kenji |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene to Cells 18, 369-386(2013)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene to Cells 18, 369-386(2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12225 His-Xpress-RALT DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The His-Xpress-RALT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12225).

Reference section:

Takeda, K., Takata, T., Kawai, Y., Ishigaki, Y., Kajinami. K,, Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12225_B1Bcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, T7 pro, QBI SP163, 6xHis, Xpress tag, insert 5'
Sequence file: RDB12225_B1Bca.seq check
>D01392A1_B1Bc_3_CMV-Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNGCNNNGGG CGGTAGGCGT GTACGGTGGG NGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTAG CGCAGAGGCT
  181 TGGGGCAGCC GAGCGGCAGC CAGGCCCCGG CCCGGGCCTC GGTTCCAGAA GGGAGAGGAG
  241 CCCGCCAAGG CGCGCAAGAG AGCGGGCTGC CTCGCAGTCC GAGCCGGAGA GGGAGCGCGA
  301 GCCGCGCCGG CCCCGGACGG CCTCCGAAAC CATGGGGGGT TCTCATCATC ATCATCATCA
  361 TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA
  421 TAAGGTACAG GCCCTTGGTT CAACAGCAGG AGTTGCTGCT CCGGATATTC GAGTCCCATT
  481 AAAAACTGGA TTTCTCCATA ATGGCCAGGC TTTGGGGAAT ATGAAGACCT GCTGGGGCAG
  541 TCGCAATGAG TTTGAAAAGA ATTTTTTAAA TATCGATCCA ATAACCATGG CCTACAATCC
  601 GAACTCCCCT GCTCCGGAGC ATCTAACAAC CCTCGGATGT GCTTCTCCGC CCGCTCCAGG
  661 GAGCGGTCAC TTCTTTGCAG AGCGTGGTCC ATCTCCAAAG TCAAGCTTGC CACCTCTTGT
  721 TACCCCACCA AGTGAAAGCT CGGGACAGCG TGAAGAGGAT CAAGTTCTGT GTGGTTTTAA
  781 GAAACTCTCA GTGAATGGGG TGTGCGCTTC CACACCTCCA CTTACACCCA TTCAAAGCTG
  841 CTCTTCCCCG TTTCCCTGTG CAGCTCCCTG NGATCGCAGT TCCCGANCGC TCCCACCATT
  901 GCCCATCTCT GANGANCCNT CTCNGNNTGA NGNCNANTGN GNNNNNNNAN TTTCNNANNN
961 NNGNANANAC NNNNNNNCTT TTNNNANNNN NGNNNGNCNN NNNNNNTNNN NNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA, insert 3'
Sequence file: RDB12225_B1Bcb.seq check
>D01392A1_B1Bc_3_BGH_rev2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG GNNGGGCANC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG
  121 ATATCTGCAG AATTCCACCA CACTGGATCC TAGGTACAGG CCCTTCTACG GAGAAACCAC
  181 GTAGGATAAG TGTTTGCGCT TCCCGTGGCC GCCCACATCC ATTTTCATTC TGGAGGCCAG
  241 CTTGTCTGTG GCAGAGACCA TACCACAGGC AGCAGGTAAT GGCTGAATCT GGGTGCTTGG
  301 GTTTGCTTCT TCTGCTTCCC TAAAATACTT CTCATATTTG TCAAGGTATG GCGGTCTCTC
  361 AGGTAGTAGG TAATAATGCG TGGAGCTGAC CTTCTTCCCA TTCTCAATGA TGGGCAGGAT
  421 GCAGGGGGCC TTGGCAGACC CTTCGCTGCT CTGTCTCTGC GGGGCTTTGC TGCTGACATA
  481 CTTGGGGTCG GGGGCAAAGC TCTGTGTTGG AGGCATGACC CCATTGAGGT AAGACGGAAG
  541 GCTTTTAGGA CTTGGAGTAC GGGAGTTACT CCGAGACAAA GGTTCTCTCG GGGGGACTTT
  601 GGGAGGCCTA TCTTCATCAC TGTATGTGTT AGAGGTCACT TCTGCTGACC ACCTTCTGTA
  661 GTCTGGCTTG GCTGGCCTGG GAGGTATAGG AACCCGGGGC GGGATCTCAG GCTTGTCTTC
  721 ATCCGAGCTA GGAGAGGCTC TGTGTGTGCA GCTAGATATC CGGATGGCTG GCTTGTTAAA
  781 CGACCCGGCT GGTCCTGANT GGGACCTCCT TAATCTGCGA TGGCTTTGAN GCGGGGGANG
  841 GTTCGGATCT GGAACAACTC TGTTCTGGTC ANATGCACAG CTAANNTCTG NCACAGAAAC
  901 AGTTGGNCTG NCAAAATACG CATAATTGNT CTGNCCNCAN CCNCNGNAGC TTCNNCNGNN
961 NNNNNNNTCN NATTNNNANT CGGNANNNNN NNNNN
//
Primer: gene33-M_F1 (Pr0947)
Region: insert mid (S250 WT)
Sequence file: RDB12225_B1Bcc.seq check
>D01392A1_B1Bc_3_gene33-M_F1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCTTANC TGTGNNNNTG ANCNGAACAG AGTTGTTCCN
   61 GATCCGAACC CTCCCCCGCC TCAAAGCCAT CGCAGATTAA GGAGGTCCCA CTCAGGACCA
  121 GCCGGGTCGT TTAACAAGCC AGCCATCCGG ATATCTAGCT GCACACACAG AGCCTCTCCT
  181 AGCTCGGATG AAGACAAGCC TGAGATCCCG CCCCGGGTTC CTATACCTCC CAGGCCAGCC
  241 AAGCCAGACT ACAGAAGGTG GTCAGCAGAA GTGACCTCTA ACACATACAG TGATGAAGAT
  301 AGGCCTCCCA AAGTCCCCCC GAGAGAACCT TTGTCTCGGA GTAACTCCCG TACTCCAAGT
  361 CCTAAAAGCC TTCCGTCTTA CCTCAATGGG GTCATGCCTC CAACACAGAG CTTTGCCCCC
  421 GACCCCAAGT ATGTCAGCAG CAAAGCCCCG CAGAGACAGA GCAGCGAAGG GTCTGCCAAG
  481 GCCCCCTGCA TCCTGCCCAT CATTGAGAAT GGGAAGAAGG TCAGCTCCAC GCATTATTAC
  541 CTACTACCTG AGAGACCGCC ATACCTTGAC AAATATGAGA AGTATTTTAG GGAAGCAGAA
  601 GAAGCAAACC CAAGCACCCA GATTCAGCCA TTACCTGCTG CCTGTGGTAT GGTCTCTGCC
  661 ACAGACAAGC TGGCCTCCAG AATGAAAATG GATGTGGGCG GCCACGGGAA GCGCAAACAC
  721 TTATCCTACG TGGTTTCTCC GTANAAGGGC CTGTACCTAG GATCCAGTGT GGTGGAATTC
  781 TGCAGATATC CAGCACAGTG GCGGCCGCTC GAGTCTAGAG GGCCCGTTTA AACCCGCTGA
  841 TCAGCCTCNA CTGTGCCTTC TANTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGNCT
  901 NNNNTGACCN NGNANNGNGC CANTCCNNCT NNCCTTTNNN NNNAAANTGA GNAAATTGCA
  961 TCGCANTNGN CNGANTNNNT GTCNNTCTAT NNNNGGGGGN NNGGGNNNGG GNNNNNNNAN
1021 NNANNGGGGG NNNGNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, EM7 pro, BleoR, SV40 pA
Sequence file: RDB12225_B1Bcd.seq check
>D01392A1_B1Bc_3_SV40pro_ori_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NGNNNNNNNN NNNNCTCTGC CTCTGNNCTA TTCCNGAAGT AGTGAGGAGG
   61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
  121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC
  181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG
  241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC
  301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC
  361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG
  421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC
  481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC
  541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC
  601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
  661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
  721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  781 TCACTGCATT CTANTTGTGN TTTGTCNAAA CTCATCAATG TATCTTATCA TGTCTGTATA
  841 CCGTCNANCT CTANCTAGAG CTTGGCGTAN TCATGGNCAT AGCTGTTNCC TGNNNGAAAT
  901 TGTTATNNGC TCACNATTCC NCACNACATA CNAANCCNNN ANNATAAAGT GTAAAGCCTG
961 GGGGNGNCNA ANGAGTGANC TNACNNNCAT TNANNNNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takeda, K., Chk1-mediated phosphorylation of receptor-associated late transducer at serine 250 increases its stability by stimulating its interaction with 14-3-3. Gene to Cells 18: 369-386 (2013). PMID 23432726. [link to RRC of NBRP]