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pMT-PURO2

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells

Catalog number RDB12152
Resource name pMT-PURO2
Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 4.9 kb
Selectable markers Amp^r
Depositor Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
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RDB12152 pMT-PURO2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12152z.seq from Depositor

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References

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12152_A5F1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB12152_A5F1a.seq
>12152_12152_A5F1_M13(-40)_G12_21.ab1
    1 CAGGAGGGGG ATTATTCGTT GCAGGACAGG ATGTGGTGCC CGATGTGACT AGCTCTTTGC
   61 TGCAGGCCGT CCTATCCTCT GGTTCCGATA AGAGACCCAG AACTCCGGCC CCCCACCGCC
  121 CACCGCCACC CCCATACATA TGTGGTACGC AAGTAAGAGT GCCTGCGCAT GCCCCATGTG
  181 CCCCACCAAG AGCTTTGCAT CCCATACAAG TCCCCAAAGT GGAGAACCGA ACCAATTCTT
  241 CGCGGGCAGA ACAAAAGCTT CTGCACACGT CTCCACTCGA ATTTGGAGCC GGCCGGCGTG
  301 TGCAAAAGAG GTGAATCGAA CGAAAGACCC GTGTGTAAAG CCGCGTTTCC AAAATGTATA
  361 AAACCGAGAG CATCTGGCCA ATGTGCATCA GTTGTGGTCA GCAGCAAAAT CAAGTGAATC
  421 ATCTCAGTGC AACTAAAGGG GGGATCCGAT CTCAATATGA AGTTATGCAT ATTACTGGCC
  481 GTCGTGGCCT TTGTTGGCCT CTCGCTCGGG AGATCTTCGC GAACTAGTCC GGATATCCAC
  541 GTGTGTACAC CTAGGCTTAA GTCTAGAGGG CCCTTCGAAG GTAAGCCTAT CCCTAACCCT
  601 CTCCTCGGTC TCGATTCTAC GCGTACCGGT CATCATCACC ATCACCATTG AGTTTAAACC
  661 CGCTGATCAG CCTCGACTGT GCCTTCTAAG GCCTGAGCTC GCTGATCAGC CTCGATCGAG
  721 GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
  781 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
  841 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
  901 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
  961 CAGTCGACCT GCAGGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
 1021 AATTGTTTAT CCGCTCACAA TTTCCACACA ACATACGAGC CGGGAAGCAT AAAGTGTAAA
 1081 GCTGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG ATGCGCCTCA CTGACCGCTT
1141 TTCCAGTCGG GAAACCTGGT TCGCG
//
Primer: COPIApro-F RDB12152_A5F1b.seq
>12152_12152_A5F1_COPIApro_F_H12_24.ab1
    1 ATGGTAGAGA TACCACTCTT TTTATTCCTT CTTTCCTTCT TGTACGTTTT TTGCTGTAAG
   61 TAGGTCGTGG TGCTGGTGTT GCAGTTGAAA TAACTTAAAA TATAAATCAT AAAACTCAAA
  121 CATAAACTTG ACTATTTATT TATTTATTAA GAAAGGAAAT ATAAATTATA AATTACAACA
  181 GATCCCCCGG GCTGCAGGAA TTCGATAGCT TGCGCCGCCA CCATGACCGA GTACAAGCCC
  241 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG
  301 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC
  361 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
  421 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
  481 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
  541 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
  601 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
  661 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
  721 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
  781 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG
  841 CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGGCTCCG ACCGAAGCCA CCCGGGGCGG
  901 CCCCGCCGAC CCCGCACCCG CCCCCGAGGC CCACCGACTC TAGATCAAGC TTATCGATAC
  961 CGTCGACCTC GAGGCCAGCT AGCTTGAACT TGTTTATTGC AGCTTATAAT GGTTTACAAA
 1021 TAAAGCAATA GCATCACAAA TTTCACAATA AGCATTTTTT TTTCACTGCA ATCTAGTGTG
 1081 GTTGTCCAAA CTCATCCATG TATCTAATCA ATGTCTGCAT CCGGCTAACT GGACGCGTGA
1141 TGCGTCAATA TCATCCGGTT AACGGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

2018.12.11

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