Resource data sheet
DNA Bank Top

pMT-PURO2 (#RDB12152)

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells


Drawn by SnapGene® software
Sequence (full) RDB12152hts01.seq
Publication Nagahashi, K., Cytotechnology, 65 (2), 173-178 (2013). [link to RRC of NBRP]
Test sheet RDB20286_B4Dhp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 4.9 kb
Selectable markers Amp^r
Growth conditions 37C, LB + Amp
Depositor|Developer Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12152 pMT-PURO2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMT-PURO2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12152).

Reference section:

Nagahashi, K., Umemura, K., Kanayama, N., Iwaki, T., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964. [link to RRC of NBRP]