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pMT-PURO2

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells

Catalog number RDB12152
Resource name pMT-PURO2
Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 4.9 kb
Selectable markers Amp^r
Depositor|Developer Iwaki, Takayuki |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12152 pMT-PURO2 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB12152zzk02.seq provided by the depositor

            Original reference

            original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12152_A3Gop1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: HB-NOS_R
            Region: MT pro, BiP signal seq, V5 tag, 6XHis, SV40pA
            Sequence file: RDB12152_A3Goa.seq check
            >12152_12152_B0J1F_HB-NOS_R_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNGNNNNNN TGGNNNNNCC NGGGTTTTCC NNGTCACGAC GTTGTAAAAC
               61 GACGGCCAGT GAATTAATTC GTTGCAGGAC AGGATGTGGT GCCCGATGTG ACTAGCTCTT
              121 TGCTGCAGGC CGTCCTATCC TCTGGTTCCG ATAAGAGACC CAGAACTCCG GCCCCCCACC
              181 GCCCACCGCC ACCCCCATAC ATATGTGGTA CGCAAGTAAG AGTGCCTGCG CATGCCCCAT
              241 GTGCCCCACC AAGAGCTTTG CATCCCATAC AAGTCCCCAA AGTGGAGAAC CGAACCAATT
              301 CTTCGCGGGC AGAACAAAAG CTTCTGCACA CGTCTCCACT CGAATTTGGA GCCGGCCGGC
              361 GTGTGCAAAA GAGGTGAATC GAACGAAAGA CCCGTGTGTA AAGCCGCGTT TCCAAAATGT
              421 ATAAAACCGA GAGCATCTGG CCAATGTGCA TCAGTTGTGG TCAGCAGCAA AATCAAGTGA
              481 ATCATCTCAG TGCAACTAAA GGGGGGATCC GATCTCAATA TGAAGTTATG CATATTACTG
              541 GCCGTCGTGG CCTTTGTTGG CCTCTCGCTC GGGAGATCTT CGCGAACTAG TCCGGATATC
              601 CACGTGTGTA CACCTAGGCT TAAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC
              661 CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA
              721 ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AAGGCCTGAG CTCGCTGATC AGCCTCGATC
              781 GANGNTCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
              841 TGAAAAAAAN GCTTTATTTG TGAAATTTGT GANGCTATTG CTTTATTTGT AANNNTTATA
              901 AGCTGCAATA AACAAGTTAA CAACNNNNAT TGCNNTCATT TTATGTTTNA NGTTCNNGGG
              961 GANGNNNGGG ANGNTTTTTT AAAGCAAGTA AAANNNNNNC NANNGTGGNN NGNNNNANTA
            1021 TGATNAGTCN NNCNNNNNNN NNN
            //
            Primer: COPIApro-F
            Region: COPIA pro, PuroR
            Sequence file: RDB12152_A3Gob.seq check
            >12152_12152_B0J1F_COPIApro_F_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNCTTTTNNT TCCTTCTTTC CTTCTTGTAC GTTTTTTGCT GTAAGTAGGT
               61 CGTGGTGCTG GTGTTGCAGT TGAAATAACT TAAAATATAA ATCATAAAAC TCAAACATAA
              121 ACTTGACTAT TTATTTATTT ATTAAGAAAG GAAATATAAA TTATAAATTA CAACAGATCC
              181 CCCGGGCTGC AGGAATTCGA TAGCTTGCGC CGCCACCATG ACCGAGTACA AGCCCACGGT
              241 GCGCCTCGCC ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC
              301 CGACTACCCC GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC GGGTCACCGA
              361 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA
              421 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT
              481 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA
              541 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT
              601 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT
              661 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT
              721 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAN GTCGAGGTGC CCGAANGANC
              781 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGACGC CNGCCCCACG ANCCGCANCG
              841 CNNGANCGAA AGGAGCGCAC GANCCNNNNN CTCCNANCNA ANCCACCCGG GGNGGNCCCG
              901 CCGANCCCGC NCCCNCCCCC NANGCCCACN GNNTCNANNT CNAGCNNNNC GATACCGNCN
            961 ANNNCNANNC CAGCTANCNN GNNNNNGNTN ANNNNANNTN ANNNNNNNNN N
            //
            Primer: Amp_R
            Region: SV40pA, PuroR
            Sequence file: RDB12152_A3Goc.seq check
            >RDB12152_1_Amp_R_A11_02.ab1
                1 NNNNNNNNNN NNNNNNNNNN NATTTATNNG GGTTATTGTC TCATGAGCGG ANACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
              241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
              301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
              361 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
              421 CGCATCAGGC GCCGGTACCC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
              481 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT
              541 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTCAAGC TAGCTGGCCT CGAGGTCGAC
              601 GGTATCGATA AGCTTGATCT AGAGTCGGTG GGCCTCGGGG GCGGGTGCGG GGTCGGCGGG
              661 GCCGCCCCGG GTGGCTTCGG TCGGAGCCAT GGGGTCGTGC GCTCCTTTCG GTCGGGCGCT
              721 GCGGGTCGTG GGGCGGGCGT CAGGCACCGG GCTTGCGGGT CATGCACCAG GTGCGCGGTC
              781 CTTCGGGCAC CTCGACGTCN GCGGTGANGG TGAANCCGAN CCGCTCGTAN AANGGGANGT
              841 TGCGGGGCGC GGANGTCTCC AGGAANGNGG GCACCCCGGC GCGCTCGGNN GNCTCCNNTC
              901 CGGGGANCAC GANNGCGCTG CCCNGACCCN TGCCCNNNNG GNCGGGNGAN NNNNNNNANN
            961 NNGGNCNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl