Resource data sheet
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pMXs-TMG2A (#RDB12142)

Retroviral vector of mouse Tbx5, Mef2c and Gata4

Clone info. Retroviral vector of mouse Tbx5, Mef2c and Gata4.
Comment Expression was confirmed with QRT-PCR and Western blotting by the depositor.
Vector backbone pMXs (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Gata4 cDNA Mouse Mef2c cDNA Mouse Tbx5 cDNA
Depositor|Developer Ieda, Masaki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR. RECIPIENT must include the DEPOSITOR as a co-author in the case of first publication based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT must describe RIKEN-RDB number in the Method section for the publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks ((Additional form)) Approval Form(FormD)
Remember that you will be working with samples containing infectious virus.
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。本件リソースを最初に使用した論文では共著を提供の条件とする。論文のマテリアルメソードでpMXs-TMG2AのRIKEN-RDB番号を明記する。学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 【追加提供依頼書】承諾書(FormD)
このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB12142 pMXs-TMG2A Under QC test. Please contact us. DNA solution Please contact us.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMXs-TMG2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12142).

Reference section:

Inagawa, K., Miyamoto, K., Yamakawa, H., Muraoka, N., Sadahiro, T., Umei, T., Wada, R., Katsumata, Y., Kaneda, R., Nakade, K., Kurihara, C., Obata, Y., Miyake, K., Fukuda, K., Ieda, M., Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ. Res., 111 (9): 1147-1156 (2012). PMID 22931955. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Inagawa, K., Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ. Res., 111 (9): 1147-1156 (2012). PMID 22931955. [link to RRC of NBRP]