RDB12141 - pMXs-GMT2A

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pMXs-GMT2A (#RDB12141)

Retroviral vector of mouse Gata4, Mef2c and Tbx5

Clone info.	Retroviral vector of mouse Gata4, Mef2C and Tbx5.
Comment	Expression was confirmed with QRT-PCR and Western blotting by the depositor.
Vector backbone	pMXs (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Gata4 cDNA Mouse Mef2c cDNA Mouse Tbx5 cDNA
Depositor\|Developer	Ieda, Masaki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR. RECIPIENT must include the DEPOSITOR as a co-author in the case of first publication based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT must describe RIKEN-RDB number in the Method section for the publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.

Remarks ((Additional form)) Approval Form(FormD)
Remember that you will be working with samples containing infectious virus.
Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR. RECIPIENT must include the DEPOSITOR as a co-author in the case of first publication based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT must describe RIKEN-RDB number in the Method section for the publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. Please obtain written approval of the depositor.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 寄託者による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。本件リソースを最初に使用した論文では共著を提供の条件とする。論文のマテリアルメソードでpMXs-GMT2A のRIKEN-RDB番号を明記する。学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 MTAに書く付加的使用条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考	【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12141	pMXs-GMT2A	DNA solution	Please contact us.

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-GMT2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12141).

Reference section:

Inagawa, K., Miyamoto, K., Yamakawa, H., Muraoka, N., Sadahiro, T., Umei, T., Wada, R., Katsumata, Y., Kaneda, R., Nakade, K., Kurihara, C., Obata, Y., Miyake, K., Fukuda, K., Ieda, M., Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ. Res., 111 (9): 1147-1156 (2012). PMID 22931955. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12141_A4F1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') Sequence file: RDB12141_A4F1a.seq
>12141_12141_A4F1_5'Retro(pFB-5')_A01_01_ABI08.ab1 1 CGTTACTAGC AGTCGTCTTA GAGCATCTCG ACTGCTAGTT ATTAAGGATC CCAGTGTGGT 61 GGTACGGGAA ATCACAAGTT TGTACAAAAA AGCAGGCTCC GCGGCCGCCC CCTTCACCAT 121 GTACCAAAGC CTGGCCATGG CCGCCAACCA CGGCCCCCCG CCCGGCGCCT ACGAAGCAGG 181 TGGCCCTGGC GCCTTCATGC ACAGCGCGGG CGCCGCGTCC TCGCCCGTCT ACGTGCCCAC 241 TCCGCGGGTG CCGTCCTCTG TGCTGGGCCT GTCCTACCTG CAGGGCGGTG GCAGTGCCGC 301 TGCAGCTGGA ACCACCTCGG GTGGCAGCTC CGGGGCCGGC CCGTCGGGTG CAGGGCCTGG 361 GACCCAGCAG GGTAGCCCTG GCTGGAGCCA AGCTGGAGCC GAGGGAGCCG CCTACACCCC 421 GCCGCCCGTG TCCCCGCGCT TCTCTTTCCC GGGGACTACT GGGTCCCTGG CGGCCGCTGC 481 CGCCGCTGCC GCAGCCCGGG AAGCTGCAGC CTACGGCAGT GGCGGCGGGG CGGCGGGCGC 541 TGGTCTGGCT GGCCGAGAGC AGTACGGGCG TCCGGGCTTC GCCGGCTCCT ACTCCAGCCC 601 CTACCCAGCC TACATGGCCG ACGTGGGAGC ATCCTGGGCC GCAGCCGCTG CCGCCTCTGC 661 CGGCCCCTTC GACAGCCCAG TCCTGCACAG CCTGCCTGGA CGGGCCAACC CTGGAAGACA 721 CCCCAATCTC GATATGTTTG ATGACTTCTC AGAAGGCAGA GAGTGTGTCA ATTGTGGGGC 781 CATGTCCACC CCACTCTGGA GGCGAGATGG GACGGGACAC TACCTGTGCA ATGCCTGTGG 841 CCTCTATCAC AAGATGAACG GCATCAACCG GGCCCCTCAT TAAGCCTCAG CGCCGCCTGT 901 CCGCTTCCCG CCGGGTAGGC CCTCTCCTGT GCCAACTGCC AGACTACCAC CACCACGCTG 961 TGGCGTCGTA ATGCCGAGGG TGAGCCTGTA TGTAATGCCT GCGGCCTCTA CATGAGCTCC 1021 ATGGGGGTTC CAAGCCCTCT TTGCAATGCG GAAGGAGGGG ATTCAACCCA GAAACGAGCC 1081 AGACCTGAAT AATCTAGACG CAGCAGTCCT TGCTTGGTGA GACCCTCCCT CCCCTTCAGA 1141 TGGGTTGTCT CTAG //
Primer: Gata4-C_F Sequence file: RDB12141_A4F1b.seq
>12141_12141_A4F1_Gata4-C_F_B01_02_ABI08.ab1 1 GTCAAAGGCA ATCTCTGTCA CTCAGACATC GCAGGCCAGC TCCAAGCAGG ACTCTTGGAA 61 CAGCCTGGTC CTGGCTGACA GTCATGGGGA CATAATCACC GCGGCTAGTG AAGGTCGTGG 121 TTCTTTATTA ACTTGTGGTG ATGTTGAAGA AAATCCTGGT CCTGCTAGTA TGGGGAGAAA 181 AAAGATTCAG ATTACGAGGA TAATGGATGA GCGTAACAGA CAGGTGACTT TTACGAAGAG 241 GAAATTTGGA TTGATGAAGA AGGCTTATGA GCTGAGCGTG CTGTGCGACT GTGAGATTGC 301 ACTGATCATC TTCAACAGCA CCAACAAGCT GTTCCAGTAC GCCAGCACTG ACATGGATAA 361 GGTGTTGCTC AAGTACACCG AGTACAACGA GCCGCACGAG AGCCGGACAA ACTCAGACAT 421 TGTGGAGGCA TTGAACAAGA AAGAAAACAA AGGCTCTGAA AGCCCCGATC CTGACTCCTC 481 TTATGCACTC ACCCCACGCA CTGAAGAAAA ATACAAAAAA ATTAATGAAG AATTTGATAA 541 TATGATCAAG AGTCATAAAA TTCCTGCTGT TCCACCTCCC AGCTTTGAGA TGCCAGTTAC 601 CATCCCAGTG TCCAGCCATA ACAGTTTGGT GTACAGCAAT CCTGTCAGCA CACTGGGAAA 661 CCCCAATCTT CTGCCACTGG CCCACCCGTC TCTGCAGAGG AATAGTATGT CTCCTGGTGT 721 AACACATAGA CCTCCAAGTG CAGGTAACAC AGGCGGTCTG ATGGGCGGAG ATCTGACATC 781 CGGTGCAGGC ACCAGCGCAG GGAATGGATA CGGCAACCCC CGGAACTCAC CAGGCCTGCT 841 GGTCTCACCT GGTAACCTGA ACAAGAATAT ACAAGCCAAA TCTCCTCCCC CTATGAATCT 901 AGGAATGAAT AATCGTAAGC CAGATCTCCG CGTTCTTATC CCACCTGGCA GCAAGGAACA 961 CGATGCCATC AGTGAATCAA AGGATAATAA CTCCCAGTCG GCTCAGTCAT TTGGGCTACC 1021 CCGGGTGGTT TCCGTAGCAA CTCCTACTTT ACCAGGACCA AGGGAATGGG AAGGAATATT 1081 TCAATCAGGC CAATTTCAAC CACATATGGT TACTGAGTTA CTCTTCTGAG GTAAGCCGCC 1141 AAGAAAGATC //
Primer: Mef2c-C_F Sequence file: RDB12141_A4F1c.seq
>12141_12141_A4F1_Mef2c-C_F_C01_03_ABI08.ab1 1 GCCAAAAATT GGGGAACGGC GAGGAGGACA CCGGAACGAA TTCCACTCCC CCATTGGACT 61 CACCAGACCT TCGCCGGACG AAAGGGAAAG TCCTTCAGTC AAGCGCATGC GACTCTCTGA 121 AGGATGGGCA ACAGCTAGTG AAGGTCGTGG TTCTTTATTA ACTTGTGGTG ATGTTGAAGA 181 AAATCCTGGT CCTGCTAGTA TGGCCGATAC AGATGAGGGC TTTGGCCTGG CGCGCACGCC 241 TCTGGAGCCT GATTCCAAAG ACAGGTCTTG CGATTCGAAA CCTGAGAGTG CTCTGGGGGC 301 TCCCAGCAAG TCTCCATCAT CCCCGCAGGC TGCCTTCACC CAGCAGGGCA TGGAAGGAAT 361 CAAGGTGTTT CTTCATGAAC GTGAACTGTG GCTGAAGTTC CACGAAGTGG GCACAGAGAT 421 GATCATCACC AAGGCAGGGA GGAGAATGTT TCCTAGTTAC AAAGTGAAGG TGACTGGCCT 481 TAATCCCAAA ACGAAGTATA TTCTTCTCAT GGATATTGTT CCCGCAGACG ACCACAGATA 541 TAAATTTGCT GATAACAAAT GGTCCGTAAC TGGCAAAGCA GAGCCTGCCA TGCCGGGGCG 601 CCTTTACGTG CACCCGGACT CCCCAGCAAC CGGAGCCCAC TGGATGCGAC AACTTGTCTC 661 CTTCCAGAAG CTCAAACTCA CCAACAACCA CCTGGACCCG TTTGGACACA TTATCCTGAA 721 CTCCATGCAC AAATACCAGC CCCGATTACA CATCGTGAAA GCAGACGAAA ATAATGGGTT 781 CGGTTCAAAG AACACTGCGT TTTGCACCCA CGTCTTCCCG GAGACAGCTT TTATCGCTGT 841 GACTTCGTAC CAGAATCACA AGATCACACA GCTGAAAATT GAGAACAACC CCTTCGCCAA 901 AGGCTTTCGG GGCAGTGATG ACCTGGAGTT ACACAGGATG TCTCGGATGC AAAGTAAAGA 961 GTAATCCTTG TGGTTTCCCA GGAGCACAGT GAGGGCACAA AGTCACCCTC CAACCACAGC 1021 CCCTTTCAGC AGCGAGACCC GAGGCTCTCT CCACCCTCAT CCCAATTTAG GGTTCCCCAG 1081 GTACCAAGTG GTGAGATTGG GTGTCTCTGG GCCCGTCCAA GGACGATCTG CCCCCACCTA 1141 AACCCCAATT ACCACTGGGC CCAGGGGAGA TCACACAG //
Primer: Tbx5-C_F Sequence file: RDB12141_A4F1d.seq
>12141_12141_A4F1_Tbx5-C_F_D01_04_ABI08.ab1 1 AAAAACACGG AATGCGGCTC AGCCCAGAGT TTCTCTACAC TCACGGGCGG TGCCCAGGGA 61 CCCTGTCCCC CCATCAGTAT CACTCGGTAC ACGGCGTCGG CATGGTGCCA GAGTGGAGTG 121 AGAATAGCTA AAAGGGTGGG CGCGCCGACC CAGCTTTCTT GTACAAAGTG GTGATATTCC 181 AGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG TCAAAAATAA TAATAACCGG 241 GCAGGCCATG TCTGCCCGTA TTTCGCGTAA GGAAATCCAT TATGTACTAT TTAAACTCGA 301 GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA 361 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG 421 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT CAGGAACAGA 481 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC 541 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG 601 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA 661 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC 721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG 781 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT 841 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG 901 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCACA 961 TGCAGCATGT ATCAAAATTA ATTTGGTTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA 1021 ATAGTTGCAT TAATGAAATC GGCCAACGCG CGGGGGAAGA AGGCGGTTTG CGTATTGGGC 1081 GCTCTTCCGC TTTCCTCGCT CACTGAACTC GCTTGCGCTC GGTTCGTCGG CTGCCGGCCG 1141 AGCGGTTATC AGCTCCAACT TCAAAAGGGC CGGGTAAATA TACGGGGCTG AT //
Primer: Amp_R Sequence file: RDB12141_A4F1e.seq
>12141_12141_A4F1_Amp_R_E01_05_ABI08.ab1 1 CCTTTTTTTT TAAATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT 181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA 241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG 301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA 361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC 421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT 481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC 541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT 601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT 661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT 721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG 781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA 841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGG CTAAACTAGC TCTGTATCTG GCGGACCCGT 901 GGTGGAACTG ACGAGTTCGG AACACCCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT 961 CGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAAATCC CGATCGTTTT GGACTCTTTG 1021 GTGCACCCCC CTTAATAGGA GGATATGTGG GTTCTGGTAA GGAGGACGAA GACCTTAAAC 1081 AGTTCCGGCT TCGGTCTGAT ATTGCTTAGT TGGACTGAAG ACCGCAACGC GCGATCATAG 1141 TTCGTCCGTC CAGACGAACT CGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Inagawa, K., Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ. Res., 111 (9): 1147-1156 (2012). PMID 22931955. [link to RRC of NBRP]