Resource data sheet
Prev.

pMXs-GMT2A

Retroviral vector of mouse Gata4, Mef2c and Tbx5

Catalog number RDB12141
Resource name pMXs-GMT2A
Clone info. Retroviral vector of mouse Gata4, Mef2C and Tbx5.
Expression was confirmed with QRT-PCR and Western blotting by the depositor.
Vector backbone pMXs (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Gata4 cDNA Mouse Mef2c cDNA Mouse Tbx5 cDNA
Depositor Ieda, Masaki |
 
Other clones in our bank mouse Gata4 (NCBI Gene 14463) | mouse Mef2c (NCBI Gene 17260) | mouse Tbx5 (NCBI Gene 21388) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR. RECIPIENT must include the DEPOSITOR as a co-author in the case of first publication based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT must describe RIKEN-RDB number in the Method section for the publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
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Remarks ((Additional form)) Approval Form(FormD)
Remember that you will be working with samples containing infectious virus.
Please obtain written approval of the depositor.
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RDB12141 pMXs-GMT2A DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Inagawa, K., Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ. Res., 111 (9): 1147-1156 (2012). PMID 22931955.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12141_A4F1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12141_A4F1a.seq
>12141_12141_A4F1_5'Retro(pFB-5')_A01_01_ABI08.ab1
    1 CGTTACTAGC AGTCGTCTTA GAGCATCTCG ACTGCTAGTT ATTAAGGATC CCAGTGTGGT
   61 GGTACGGGAA ATCACAAGTT TGTACAAAAA AGCAGGCTCC GCGGCCGCCC CCTTCACCAT
  121 GTACCAAAGC CTGGCCATGG CCGCCAACCA CGGCCCCCCG CCCGGCGCCT ACGAAGCAGG
  181 TGGCCCTGGC GCCTTCATGC ACAGCGCGGG CGCCGCGTCC TCGCCCGTCT ACGTGCCCAC
  241 TCCGCGGGTG CCGTCCTCTG TGCTGGGCCT GTCCTACCTG CAGGGCGGTG GCAGTGCCGC
  301 TGCAGCTGGA ACCACCTCGG GTGGCAGCTC CGGGGCCGGC CCGTCGGGTG CAGGGCCTGG
  361 GACCCAGCAG GGTAGCCCTG GCTGGAGCCA AGCTGGAGCC GAGGGAGCCG CCTACACCCC
  421 GCCGCCCGTG TCCCCGCGCT TCTCTTTCCC GGGGACTACT GGGTCCCTGG CGGCCGCTGC
  481 CGCCGCTGCC GCAGCCCGGG AAGCTGCAGC CTACGGCAGT GGCGGCGGGG CGGCGGGCGC
  541 TGGTCTGGCT GGCCGAGAGC AGTACGGGCG TCCGGGCTTC GCCGGCTCCT ACTCCAGCCC
  601 CTACCCAGCC TACATGGCCG ACGTGGGAGC ATCCTGGGCC GCAGCCGCTG CCGCCTCTGC
  661 CGGCCCCTTC GACAGCCCAG TCCTGCACAG CCTGCCTGGA CGGGCCAACC CTGGAAGACA
  721 CCCCAATCTC GATATGTTTG ATGACTTCTC AGAAGGCAGA GAGTGTGTCA ATTGTGGGGC
  781 CATGTCCACC CCACTCTGGA GGCGAGATGG GACGGGACAC TACCTGTGCA ATGCCTGTGG
  841 CCTCTATCAC AAGATGAACG GCATCAACCG GGCCCCTCAT TAAGCCTCAG CGCCGCCTGT
  901 CCGCTTCCCG CCGGGTAGGC CCTCTCCTGT GCCAACTGCC AGACTACCAC CACCACGCTG
  961 TGGCGTCGTA ATGCCGAGGG TGAGCCTGTA TGTAATGCCT GCGGCCTCTA CATGAGCTCC
 1021 ATGGGGGTTC CAAGCCCTCT TTGCAATGCG GAAGGAGGGG ATTCAACCCA GAAACGAGCC
 1081 AGACCTGAAT AATCTAGACG CAGCAGTCCT TGCTTGGTGA GACCCTCCCT CCCCTTCAGA
1141 TGGGTTGTCT CTAG
//
Primer: Gata4-C_F RDB12141_A4F1b.seq
>12141_12141_A4F1_Gata4-C_F_B01_02_ABI08.ab1
    1 GTCAAAGGCA ATCTCTGTCA CTCAGACATC GCAGGCCAGC TCCAAGCAGG ACTCTTGGAA
   61 CAGCCTGGTC CTGGCTGACA GTCATGGGGA CATAATCACC GCGGCTAGTG AAGGTCGTGG
  121 TTCTTTATTA ACTTGTGGTG ATGTTGAAGA AAATCCTGGT CCTGCTAGTA TGGGGAGAAA
  181 AAAGATTCAG ATTACGAGGA TAATGGATGA GCGTAACAGA CAGGTGACTT TTACGAAGAG
  241 GAAATTTGGA TTGATGAAGA AGGCTTATGA GCTGAGCGTG CTGTGCGACT GTGAGATTGC
  301 ACTGATCATC TTCAACAGCA CCAACAAGCT GTTCCAGTAC GCCAGCACTG ACATGGATAA
  361 GGTGTTGCTC AAGTACACCG AGTACAACGA GCCGCACGAG AGCCGGACAA ACTCAGACAT
  421 TGTGGAGGCA TTGAACAAGA AAGAAAACAA AGGCTCTGAA AGCCCCGATC CTGACTCCTC
  481 TTATGCACTC ACCCCACGCA CTGAAGAAAA ATACAAAAAA ATTAATGAAG AATTTGATAA
  541 TATGATCAAG AGTCATAAAA TTCCTGCTGT TCCACCTCCC AGCTTTGAGA TGCCAGTTAC
  601 CATCCCAGTG TCCAGCCATA ACAGTTTGGT GTACAGCAAT CCTGTCAGCA CACTGGGAAA
  661 CCCCAATCTT CTGCCACTGG CCCACCCGTC TCTGCAGAGG AATAGTATGT CTCCTGGTGT
  721 AACACATAGA CCTCCAAGTG CAGGTAACAC AGGCGGTCTG ATGGGCGGAG ATCTGACATC
  781 CGGTGCAGGC ACCAGCGCAG GGAATGGATA CGGCAACCCC CGGAACTCAC CAGGCCTGCT
  841 GGTCTCACCT GGTAACCTGA ACAAGAATAT ACAAGCCAAA TCTCCTCCCC CTATGAATCT
  901 AGGAATGAAT AATCGTAAGC CAGATCTCCG CGTTCTTATC CCACCTGGCA GCAAGGAACA
  961 CGATGCCATC AGTGAATCAA AGGATAATAA CTCCCAGTCG GCTCAGTCAT TTGGGCTACC
 1021 CCGGGTGGTT TCCGTAGCAA CTCCTACTTT ACCAGGACCA AGGGAATGGG AAGGAATATT
 1081 TCAATCAGGC CAATTTCAAC CACATATGGT TACTGAGTTA CTCTTCTGAG GTAAGCCGCC
1141 AAGAAAGATC
//
Primer: Mef2c-C_F RDB12141_A4F1c.seq
>12141_12141_A4F1_Mef2c-C_F_C01_03_ABI08.ab1
    1 GCCAAAAATT GGGGAACGGC GAGGAGGACA CCGGAACGAA TTCCACTCCC CCATTGGACT
   61 CACCAGACCT TCGCCGGACG AAAGGGAAAG TCCTTCAGTC AAGCGCATGC GACTCTCTGA
  121 AGGATGGGCA ACAGCTAGTG AAGGTCGTGG TTCTTTATTA ACTTGTGGTG ATGTTGAAGA
  181 AAATCCTGGT CCTGCTAGTA TGGCCGATAC AGATGAGGGC TTTGGCCTGG CGCGCACGCC
  241 TCTGGAGCCT GATTCCAAAG ACAGGTCTTG CGATTCGAAA CCTGAGAGTG CTCTGGGGGC
  301 TCCCAGCAAG TCTCCATCAT CCCCGCAGGC TGCCTTCACC CAGCAGGGCA TGGAAGGAAT
  361 CAAGGTGTTT CTTCATGAAC GTGAACTGTG GCTGAAGTTC CACGAAGTGG GCACAGAGAT
  421 GATCATCACC AAGGCAGGGA GGAGAATGTT TCCTAGTTAC AAAGTGAAGG TGACTGGCCT
  481 TAATCCCAAA ACGAAGTATA TTCTTCTCAT GGATATTGTT CCCGCAGACG ACCACAGATA
  541 TAAATTTGCT GATAACAAAT GGTCCGTAAC TGGCAAAGCA GAGCCTGCCA TGCCGGGGCG
  601 CCTTTACGTG CACCCGGACT CCCCAGCAAC CGGAGCCCAC TGGATGCGAC AACTTGTCTC
  661 CTTCCAGAAG CTCAAACTCA CCAACAACCA CCTGGACCCG TTTGGACACA TTATCCTGAA
  721 CTCCATGCAC AAATACCAGC CCCGATTACA CATCGTGAAA GCAGACGAAA ATAATGGGTT
  781 CGGTTCAAAG AACACTGCGT TTTGCACCCA CGTCTTCCCG GAGACAGCTT TTATCGCTGT
  841 GACTTCGTAC CAGAATCACA AGATCACACA GCTGAAAATT GAGAACAACC CCTTCGCCAA
  901 AGGCTTTCGG GGCAGTGATG ACCTGGAGTT ACACAGGATG TCTCGGATGC AAAGTAAAGA
  961 GTAATCCTTG TGGTTTCCCA GGAGCACAGT GAGGGCACAA AGTCACCCTC CAACCACAGC
 1021 CCCTTTCAGC AGCGAGACCC GAGGCTCTCT CCACCCTCAT CCCAATTTAG GGTTCCCCAG
 1081 GTACCAAGTG GTGAGATTGG GTGTCTCTGG GCCCGTCCAA GGACGATCTG CCCCCACCTA
1141 AACCCCAATT ACCACTGGGC CCAGGGGAGA TCACACAG
//
Primer: Tbx5-C_F RDB12141_A4F1d.seq
>12141_12141_A4F1_Tbx5-C_F_D01_04_ABI08.ab1
    1 AAAAACACGG AATGCGGCTC AGCCCAGAGT TTCTCTACAC TCACGGGCGG TGCCCAGGGA
   61 CCCTGTCCCC CCATCAGTAT CACTCGGTAC ACGGCGTCGG CATGGTGCCA GAGTGGAGTG
  121 AGAATAGCTA AAAGGGTGGG CGCGCCGACC CAGCTTTCTT GTACAAAGTG GTGATATTCC
  181 AGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG TCAAAAATAA TAATAACCGG
  241 GCAGGCCATG TCTGCCCGTA TTTCGCGTAA GGAAATCCAT TATGTACTAT TTAAACTCGA
  301 GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA
  361 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
  421 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT CAGGAACAGA
  481 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  541 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
  601 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA
  661 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
  721 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  781 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT
  841 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
  901 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCACA
  961 TGCAGCATGT ATCAAAATTA ATTTGGTTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA
 1021 ATAGTTGCAT TAATGAAATC GGCCAACGCG CGGGGGAAGA AGGCGGTTTG CGTATTGGGC
 1081 GCTCTTCCGC TTTCCTCGCT CACTGAACTC GCTTGCGCTC GGTTCGTCGG CTGCCGGCCG
1141 AGCGGTTATC AGCTCCAACT TCAAAAGGGC CGGGTAAATA TACGGGGCTG AT
//
Primer: Amp_R RDB12141_A4F1e.seq
>12141_12141_A4F1_Amp_R_E01_05_ABI08.ab1
    1 CCTTTTTTTT TAAATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
  181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
  241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
  361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
  421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
  481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
  541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
  601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT
  661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT
  721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG
  781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA
  841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGG CTAAACTAGC TCTGTATCTG GCGGACCCGT
  901 GGTGGAACTG ACGAGTTCGG AACACCCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT
  961 CGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAAATCC CGATCGTTTT GGACTCTTTG
 1021 GTGCACCCCC CTTAATAGGA GGATATGTGG GTTCTGGTAA GGAGGACGAA GACCTTAAAC
 1081 AGTTCCGGCT TCGGTCTGAT ATTGCTTAGT TGGACTGAAG ACCGCAACGC GCGATCATAG
1141 TTCGTCCGTC CAGACGAACT CGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Inagawa, K., Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ. Res., 111 (9): 1147-1156 (2012). PMID 22931955.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl