Resource data sheet
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CSII-EF-mRFP1-RfA

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.

Catalog number RDB12128
Resource name CSII-EF-mRFP1-RfA
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |

Distribution information

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Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Use DB3.1(Invitrogen) or equivalent to amplify this clone.
Please contact us. We will provide you with appropriate documents.
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Sequence information

References and tips

Electronic file

References

user report Ikeda, M., Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-Dependent Manner. Mol. Cell. Biol. 37 (5). pii: e00417-16 (2016). PMID 27956699.

2018.03.15

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