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pPyCAG-CreGR-IB

Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. Blasticidin S resistance.

Catalog number RDB12117
Resource name pPyCAG-CreGR-IB
Alternative name stock no. 10489
Clone info. Expression clone of Cre-GR LBD fusion regulatable by dexamethasone.Blasticidin S resistance.
Vector backbone pPyCAG-IB (Plasmid)
Gene/insert name P1 phage Cre genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank P1 phage cre |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB12117 pPyCAG-CreGR-IB DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB12117z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB12117p.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14288_A6Gqp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14288_A6Gqa.seq check
>RDB12117_14288_A6Gq_pAxCA_F1_G06_21_ABI24.ab1
    1 CGTTTGTAAC ATCTGCTCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGAGCCA CCATGTCCAA
  121 TTTACTGACC GTACACCAAA ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT
  181 TCGCAAGAAC CTGATGGACA TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA
  241 AATGCTTCTG TCCGTTTGCC GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG
  301 GTTTCCCGCA GAACCTGAAG ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT
  361 GGCAGTAAAA ACTATCCAGC AACATTTGGG GCCAGCTAAA ACATGCTTCA TCGTCGGGTC
  421 CCGGGCTGCC ACGACCAAAG TGACAGCAAT GCTGTTTCAC TGGTTATGCG GGCGGATCCG
  481 AAAAAAAAAC GTTGAAGGCC GGTGAAACGT TCAAAAAAGG CTTCTAGCGT TCGAACGCAC
  541 TGAATTTCGA CCAAGTTCGT TTCCCCCTTG GAAAATAAGC GATCGCCGGC CAGAATAAAA
  601 CGTAACCGGG ACTTTTTGGG GGATTTGCTT AAAAACCCCC GTGTTACGTA AGCCCAAAAT
  661 TTCCCAGAAT CAGGGGTTAA AAAAATTTTC CCCTTCTGGC CGGGGGGAAA AAAGTTAATC
  721 CCTTTTTGGC CAAAACAAAA AACCCTGGTT TCCCCCCCCG GGGGTAAAAA AGGCCCTTTA
  781 CCCTGGGGGG AAAAAAAACG GGGCCCAGGC AGGGGATTCC CCCCCCTCGG GGGGACCCAA
  841 AAATCCCCAA AAATTCCCGT GGTTTCTGCG GGGGCAAAAA AAATGGGTGT GTGCGCGCGC
  901 ATATTGTCGC CACACGACAG TTTATACTCT CGCGCCCCGG GGAGGGGGGT ATTTTTTAAA
  961 GACACCCTCC CCTAGTGTTT ACACGGCGCA CACGAGAGAA TACACTGCTC GTGCAGAAAA
 1021 ACACTCGCGG CGGTGTGGGG AGAACACAAA TCTCCGCCTG TGATGAGGAG CACGCCCGCA
 1081 GATGAGGTAG TAGCTCGCTC GTGATGATAA TATATCTACA CATCACGAAC AGCATGCGAT
1141 CGATG
//
Primer: IRES-R
Sequence file: RDB14288_A6Gqb.seq check
>RDB12117_14288_A6Gq_IRES-R_G05_20_ABI24.ab1
    1 GCAACGGCGG CTTCGGCCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGCGGAA
   61 TTCGATATCA AGCTTATCGA GCGGCCGCTC ACTTTTGATG AAACAGAAGT TTTTTGATAT
  121 TTCCATTTGA ATATTTTGGT ATCTGATTGG TGATGATTTC AGCTAACATC TCGGGGAACT
  181 CGATGGACAT GGTCTTATCC AAAAATGTTT GGAAGCAATA GTTAAGGAGA TTTTCAACCA
  241 CTTCATGCAT AGAATCCAAG AGTTTTGTCA GTTGATAAAA CCGCTGCCAG TTCTGGCTGG
  301 AGTTTCCTTC CCTCTTGACA ATGGCTTTTC CTAGCTCTTT GATGTAGGTC ATTCTAATTT
  361 CATCAAATAG CTCTTGGCTC TTCAGACCGT CCTTAGGAAC TGAAGAGAGA AGCAGTAAGG
  421 TTTTCATACA GAGATACTCT TCATAAGATA CCTGAAGCCT GTGTAACTCA GAGGAAACAT
  481 ACAGCATGTG TTTACATTGG TCGTACATGC AGGGTAGAGT CATTCTCTGC TCATTAATAA
  541 TCAGATCAGG AGCAAAACAC AGCAGGTTTG CACTTGATTG TCTATATGAT CTCCACCCCA
  601 GAGCAAATGC CATAAGAAAC ATCCAGGAGT ACTGCAGTAG GGTCATTTGG TCATCCAGGT
  661 GTAAGTTCCT GAAACCTGGT ATTGCCTTTG CCCATTTCAC TGCTGCAATC ACTTGCCGCC
  721 CTCCTAACAT GTTGAGCGTA GTCATGATCC TCCAAGTTGA GTCTGGAACA GAGCTATCAT
  781 ATCCTGCATA TAACACTTCA GGTTCAATAA CCTCCAACAG TGACACCAGG GTAGGGGTGA
  841 GTTGTGGTAA CGTTGCAGGA ACTATTGTTT TGTTACCAGG ATTTTCCATG GCATAATCGC
  901 CATCTTCCAG CAAGGCGCAC CATTGCCCCT GTTTCACTAT CCCAGGTTAC GGATATAGTT
  961 CATGACAATA TTTACATTGG TCCCAGCCCA CCAGCTTGCA TGAATCTCCG GTATTGAACT
 1021 CCAGCGCGGG CCCATATCTC GCCGCGCTCG ACACGGGCAC TGTGTCCAGA ACAAGCCAGG
1081 TATCTCTGGA ACCAGAAGTT CATCATTAAG CGTCGGTAAT CAATCCGAAA ATGAAGCTG
//
Primer: IRES-F
Sequence file: RDB14288_A6Gqc.seq check
>RDB12117_14288_A6Gq_IRES-F_D08_11_ABI24.ab1
    1 GCAGTCATTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGCCTT TGTCTCAAGA
  121 AGAATCCACC CTCATTGAAA GAGCAACGGC TACAATCAAC AGCATCCCCA TCTCTGAAGA
  181 CTACAGCGTC GCCAGCGCAG CTCTCTCTAG CGACGGCCGC ATCTTCACTG GTGTCAATGT
  241 ATATCATTTT ACTGGGGGAC CTTGTGCAGA ACTCGTGGTG CTGGGCACTG CTGCTGCTGC
  301 GGCAGCTGGC AACCTGACTT GTATCGTCGC GATCGGAAAT GAGAACAGGG GCATCTTGAG
  361 CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT CGATCTGCAT CCTGGGATCA AAGCCATAGT
  421 GAAGGACAGT GATGGACAGC CGACGGCAGT TGGGATTCGT GAATTGCTGC CCTCTGGTTA
  481 TGTGTGGGAG GGTTAACTAG ATAAGTAATG ATCATAATCA GCCATATCAC ATCTGTAGAG
  541 GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT
  601 GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
  661 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
  721 CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA ATGAATCGGC CAACGCGCGG
  781 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
  841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
  901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
  961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
 1021 CACAAAAATC GGACGCTCAA AGTCAGGAGG TGGGCGAACC CCGGACCAGG AACTATAAGG
 1081 AATACAGGCG GTTTCCCCCT GGAGCTCCCC TCGGTGCGCG CCTTCTCCTG GTTTCCGGAA
1141 CCCTTGGCCC CGCCCA
//
Primer: Amp_R
Sequence file: RDB14288_A6Gqd.seq check
>RDB12117_14288_A6Gq_Amp_R_E08_14_ABI24.ab1
    1 TTCGTTTCAT GACGCTTTGG AAACAAAGAC TGTATTTCCT GGAAATTAAT GTTTATTCAA
   61 TAAACTGTGT ATTCAGCTAT ATTCACATAG TGGTGAGGCT GAAATGAGGC GGGAAGAGGC
  121 GGTTGGGGCT TAATTATATC AATTTGGGTG GCCCCACAGC GCCTCCAAGG CGCCAGTCCT
  181 GTTTTGACAA GTTGCCTCTG GAAGCCTCTC TTCTTTTTCT CCAGAGTAAG CGGAGGCCAG
  241 GGGCCCCCGG CCTCTGCTTA ATACTAAAAA AAACAGCTGT TGTCATAGTA ATGATTGGGT
  301 GGAAACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC TCTTGCAAAA CCACACTGAC
  361 ATTCCAGGCC TGGGTGGAGA GGCTTTTTGC TTCCTCTTGC AAAACCACAC TGCCCTCTGG
  421 AGGGCAGTTG CCTAGCAACT AATTAAAAGA GGATGTCGCA CGGCCAGCTG CGGTCAGTTA
  481 GTCACTTCCT GCTTAACTGA CTTGACATTT TCTATTTTAA GAGTCGGGAG GAAAATTACT
  541 GTGTTGGAGG CCCTCCGCCA TCTTCTGAAG CTGAATCGAA TTAACTTGTT TATTGCAGCT
  601 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTTC
  661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG
  721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT
  781 AGTTCATAGC CCAATATAAT GGAGTTTCCG CGTTAACATA ACTTACGTAA TGACCCGCCT
  841 GCTGACCGCC CAACGACCCC CGCCATGACG TCAATATGAC GTATGTTCCA TAGTACGCCA
  901 ATAGGGACTT CATGACGTCA TGGTTGGAAG TATTACGTAA CTGGACACTG CCAGTACATT
  961 CAGTGTATCC TATGGCAGTA CGCCCATTGA CTCATGACGT ATTGCGCTGC ATAGCCAGTA
1021 CATGACTATG GACTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

GNP_filter3_RDBDEP_html_200316.pl