Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-BstXI-ZeoP2AEGFP (#RDB12113)

Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker.

Clone info.	Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker.
Vector backbone	Plasmid
Gene/insert name	Aequorea victoria GFP cDNA
Depositor|Developer	Niwa, Hitoshi |

 

Sequence	RDB12113z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12113	pPyCAG-BstXI-ZeoP2AEGFP	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pPyCAG-BstXI-ZeoP2AEGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12113).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12113_A3E9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584) Region: CAG pro,BstXI stuffer 5' Sequence file: RDB12113_A3E9a.seq
>12113_12113_B2DdF_2_CAG_R1_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG    61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC   121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG   181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC   241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC   301 AAAGAATTCC TCGAGATCCA TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC   361 GGGCATTCCC GATCAGAAAT GAGCGCCAGT NNNNNNCGGC TCTCGGCACC GAATGCGTAT   421 GATTCTNNNN NNNCATGNNT TCNGNNANTG CGNNNNNNAN NNNNCGCTNN NNCCTGANNN //
Primer: QUEEN-M_R (Pr0808) Region: EGFP 5',P2A,Zeo,IRES Sequence file: RDB12113_A3E9b.seq
>12113_12113_B2DmF_QUEEN-M_R_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNGCCGGN NNNGCTGNAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG    61 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGGGCCGGGG   121 TTCTCCTCCA CGTCGCCGGC CTGCTTCAGC AGGGAGAAGT TGGTGGCATG CATGTCCTGC   181 TCCTCGGCCA CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC   241 CACGGCTGCT CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC   301 ACGACCTCCG ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG   361 GTGTTGTCCG GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG   421 ACCACACCGG CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG   481 AACTCGACCG CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG 541 GCCATGGTTG TGGCCATATT ATCATCGTGT TTTTCAAAGG NAAAACNNNN NNNNNN //
Primer: IRES-M_R (Pr0406) Region: IRES,BstXI stuffer 3' Sequence file: RDB12113_A3E9c.seq
>12113_12113_B2DmF_2_IRES-M_R_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNCTTC CTTNNCGACN TTCNACAGAC CTTGCATTCC TTTGGCGAGA    61 GGGGAAAGAC CCCTAGGAAT GCTCGTCAAG AAGACAGGGC CAGGTTTCCG GGCCCTCACA   121 TTGCCAAAAG ACGGCAATAT GGTGGAAAAT AACCGCGGCC GCTCGATACC GTCGATCTCA   181 GTGGTATTTG TGAGCCAGTT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC   241 TGAGGAGTGA ATTCCTCGAG ATCCATTGTG CTGGCGCGGA TTCTTTATCA CTGATAAGTT   301 GGTGGACATA TTATGTTTAT CAGTGATAAA GTGTCAAGCA TGACAAAGTT GCAGCCGAAT   361 ACAGTGATCC GTGCCGCCCT GGACCTGTTG AACGAGGTCG GCGTAGACGG TCTGACGACA   421 CGCAAACTGG CGGAACGGTT GGGGGTTCAG CAGCCGGCGC TTTACTGGCA CTTCAGGAAC   481 AAGCGGGCGC TGCTCGACGC ACTGGCCGAA GCCATGCTGG CGGAGAATCA TACGCATTCG   541 GTGCCGAGAG CCGACGACGA CTGGCGCTCA TTTCTGATCG GGANNGCNCG CNGCTTCANG   601 CNNGCGCTGC TCGNCTACNG NCNGCACNNT GNNNNNCNNN GANTNCTTTG NNNAATGNNG 661 ANNNNGCNNN NTAANCNNNN NGNTGCNNNN ANCTGNNNNA AAANNNNNAN NNNNNNNNN //
Primer: EGFP-M_R (Pr0743) Region: eEGFP 5',P2A 3' Sequence file: RDB12113_A3E9d.seq
>12113_12113_B2DmF_EGFP-M_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNGTGNNG GNNCTTGNNG TTNNCCTTGA TGCCGTTCTT CTGCTTGTCG    61 GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG   121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG   181 AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC   241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG   301 TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG   361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC   421 TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG   481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGGGCC GGGGTTCTCC   541 TCCACGTCNN CGGCCTGCTT CAGCAGGGNN NNNNTGGTGG CATGCATGTC CTGCTCNTCN 601 GNCACNNNNN NNNCGCANTT GNCNNNNNGN NNNCGCANGN NNAACTCCNN NNNNNNNN //
Primer: VN_F1 (Pr0992) Region: EGFP 3' Sequence file: RDB12113_A3E9e.seq
>12113_12113_B2DdF_2_VN_F1_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNCNACGTC NATATNNTGG CCGACAAGCA GAAGAACGGC    61 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC   121 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC   181 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG   241 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAATCG   301 ATGATCTANA GCTCGCTGAT CNGCCTCNNN NNNNNCTTCT AGTTGCCAGC CNTCTGTTGT   361 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GNAAGGTGCC NNTCNCNNTG NNNNNTCNNA 421 NNNNNNTGNN NANNNNGNNN NNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',bGH pA Sequence file: RDB12113_A3E9f.seq
>12113_12113_B2DmF_mVenus-M_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNTNNAGG TGNNNTTNNN GNNCCGCCAC AACATCGAGG ACGGCAGCGT    61 GCANCTCNNN NACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCN TGNTGCTGCC   121 CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA   181 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT   241 GTACAAGTAA TCGATGATCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC   301 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA   361 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGNNNNNNNN   421 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC   481 ATGCTGGGGA TGCGGTGGGC TCTATGGNTT CTGAGGCGGA AAGAANCTGC AGCCNNNNNN   541 NGNCNNAATC ATGGTCATAN NTGNTNNNNG NGTGAANNNN NNNNNCGCTC NNNNNNNNNN //
Primer: Amp_R (Pr0008) Region: polyoma ori Sequence file: RDB12113_A3E9g.seq
>12113_12113_B2DmF_Amp_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNGCTTT GGNNNNNNNA GANTGTATTT CCTGGAAATT AATGTTTATT    61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA   121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT   181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC   241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG   301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT   361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC   421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG   481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTN NNNNAGTCGG GAGGAAAATT   541 ACTGTGTTGG AGGCCCTCCG CCANNNNNNG AANCTGAATC GAATTAACTT GNTTATTGCA 601 NCTTANNNNG NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,SV40 pA Sequence file: RDB12113_A3E9h.seq
>12113_12113_B2DdF_2_SV40pro_ori_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNCCNCN NNNNNTGANC NATTCNNGAA GTAGTGAGGA    61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTAACTT GTTTATTGCA GCTTATAATG   121 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT   181 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA   241 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC   301 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCNNNNNNN   361 NGNGNNNNNA NNNNNNNNCA CANANNNNNG GGANANCNNA GGAAAGAACA TGTGANCAAA 421 ANGNCNNCNA ANNGNCANNA ANCNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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External Database
Aequorea victoria GFP

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