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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-BstXI-ZeoP2AEGFP

Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker.

Catalog number RDB12113
Resource name pPyCAG-BstXI-ZeoP2AEGFP
Clone info. Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker.
Vector backbone Plasmid
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12113 pPyCAG-BstXI-ZeoP2AEGFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB12113z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12113_A3E9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CAG_R1
            Region: CAG pro,BstXI stuffer 5'
            Sequence file: RDB12113_A3E9a.seq check
            >12113_12113_B2DdF_2_CAG_R1_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG
               61 AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC
              121 CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG
              181 TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC
              241 TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC
              301 AAAGAATTCC TCGAGATCCA TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC
              361 GGGCATTCCC GATCAGAAAT GAGCGCCAGT NNNNNNCGGC TCTCGGCACC GAATGCGTAT
              421 GATTCTNNNN NNNCATGNNT TCNGNNANTG CGNNNNNNAN NNNNCGCTNN NNCCTGANNN
            //
            Primer: QUEEN-M_R
            Region: EGFP 5',P2A,Zeo,IRES
            Sequence file: RDB12113_A3E9b.seq check
            >12113_12113_B2DmF_QUEEN-M_R_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNGCCGGN NNNGCTGNAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG
               61 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGGGCCGGGG
              121 TTCTCCTCCA CGTCGCCGGC CTGCTTCAGC AGGGAGAAGT TGGTGGCATG CATGTCCTGC
              181 TCCTCGGCCA CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC
              241 CACGGCTGCT CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC
              301 ACGACCTCCG ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG
              361 GTGTTGTCCG GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG
              421 ACCACACCGG CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG
              481 AACTCGACCG CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG
            541 GCCATGGTTG TGGCCATATT ATCATCGTGT TTTTCAAAGG NAAAACNNNN NNNNNN
            //
            Primer: IRES-M_R
            Region: IRES,BstXI stuffer 3'
            Sequence file: RDB12113_A3E9c.seq check
            >12113_12113_B2DmF_2_IRES-M_R_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNCTTC CTTNNCGACN TTCNACAGAC CTTGCATTCC TTTGGCGAGA
               61 GGGGAAAGAC CCCTAGGAAT GCTCGTCAAG AAGACAGGGC CAGGTTTCCG GGCCCTCACA
              121 TTGCCAAAAG ACGGCAATAT GGTGGAAAAT AACCGCGGCC GCTCGATACC GTCGATCTCA
              181 GTGGTATTTG TGAGCCAGTT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
              241 TGAGGAGTGA ATTCCTCGAG ATCCATTGTG CTGGCGCGGA TTCTTTATCA CTGATAAGTT
              301 GGTGGACATA TTATGTTTAT CAGTGATAAA GTGTCAAGCA TGACAAAGTT GCAGCCGAAT
              361 ACAGTGATCC GTGCCGCCCT GGACCTGTTG AACGAGGTCG GCGTAGACGG TCTGACGACA
              421 CGCAAACTGG CGGAACGGTT GGGGGTTCAG CAGCCGGCGC TTTACTGGCA CTTCAGGAAC
              481 AAGCGGGCGC TGCTCGACGC ACTGGCCGAA GCCATGCTGG CGGAGAATCA TACGCATTCG
              541 GTGCCGAGAG CCGACGACGA CTGGCGCTCA TTTCTGATCG GGANNGCNCG CNGCTTCANG
              601 CNNGCGCTGC TCGNCTACNG NCNGCACNNT GNNNNNCNNN GANTNCTTTG NNNAATGNNG
            661 ANNNNGCNNN NTAANCNNNN NGNTGCNNNN ANCTGNNNNA AAANNNNNAN NNNNNNNNN
            //
            Primer: EGFP-M_R
            Region: eEGFP 5',P2A 3'
            Sequence file: RDB12113_A3E9d.seq check
            >12113_12113_B2DmF_EGFP-M_R_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNGTGNNG GNNCTTGNNG TTNNCCTTGA TGCCGTTCTT CTGCTTGTCG
               61 GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG
              121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG
              181 AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC
              241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG
              301 TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG
              361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC
              421 TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG
              481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGGGCC GGGGTTCTCC
              541 TCCACGTCNN CGGCCTGCTT CAGCAGGGNN NNNNTGGTGG CATGCATGTC CTGCTCNTCN
            601 GNCACNNNNN NNNCGCANTT GNCNNNNNGN NNNCGCANGN NNAACTCCNN NNNNNNNN
            //
            Primer: VN_F1
            Region: EGFP 3'
            Sequence file: RDB12113_A3E9e.seq check
            >12113_12113_B2DdF_2_VN_F1_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNG NNNCNACGTC NATATNNTGG CCGACAAGCA GAAGAACGGC
               61 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
              121 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
              181 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
              241 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAATCG
              301 ATGATCTANA GCTCGCTGAT CNGCCTCNNN NNNNNCTTCT AGTTGCCAGC CNTCTGTTGT
              361 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GNAAGGTGCC NNTCNCNNTG NNNNNTCNNA
            421 NNNNNNTGNN NANNNNGNNN NNNNNNN
            //
            Primer: mVenus-M_F
            Region: EGFP 3',bGH pA
            Sequence file: RDB12113_A3E9f.seq check
            >12113_12113_B2DmF_mVenus-M_F_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNTNNAGG TGNNNTTNNN GNNCCGCCAC AACATCGAGG ACGGCAGCGT
               61 GCANCTCNNN NACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCN TGNTGCTGCC
              121 CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA
              181 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT
              241 GTACAAGTAA TCGATGATCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC
              301 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA
              361 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGNNNNNNNN
              421 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC
              481 ATGCTGGGGA TGCGGTGGGC TCTATGGNTT CTGAGGCGGA AAGAANCTGC AGCCNNNNNN
              541 NGNCNNAATC ATGGTCATAN NTGNTNNNNG NGTGAANNNN NNNNNCGCTC NNNNNNNNNN
            //
            Primer: Amp_R
            Region: polyoma ori
            Sequence file: RDB12113_A3E9g.seq check
            >12113_12113_B2DmF_Amp_R_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNGCTTT GGNNNNNNNA GANTGTATTT CCTGGAAATT AATGTTTATT
               61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
              121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
              181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
              241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
              301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
              361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
              421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
              481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTN NNNNAGTCGG GAGGAAAATT
              541 ACTGTGTTGG AGGCCCTCCG CCANNNNNNG AANCTGAATC GAATTAACTT GNTTATTGCA
            601 NCTTANNNNG NNNNNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,SV40 pA
            Sequence file: RDB12113_A3E9h.seq check
            >12113_12113_B2DdF_2_SV40pro_ori_F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCNCCNCN NNNNNTGANC NATTCNNGAA GTAGTGAGGA
               61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTAACTT GTTTATTGCA GCTTATAATG
              121 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT
              181 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA
              241 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC
              301 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCNNNNNNN
              361 NGNGNNNNNA NNNNNNNNCA CANANNNNNG GGANANCNNA GGAAAGAACA TGTGANCAAA
            421 ANGNCNNCNA ANNGNCANNA ANCNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl