Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-BstXI-HygP2AEGFP (#RDB12108)

Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker.

Clone info.	Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker.
Vector backbone	Plasmid
Gene/insert name	Aequorea victoria GFP cDNA
Depositor|Developer	Niwa, Hitoshi |

 

Sequence	RDB12108z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms

Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12108	pPyCAG-BstXI-HygP2AEGFP	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pPyCAG-BstXI-HygP2AEGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12108).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12108_A3E7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F (Pr0036) Region: IRES,HygR 5' Sequence file: RDB12108_A3E7a.seq
>12108_12108_A3E7_1_IRES-F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNGTCGANGT TNNAAAAACG TCTAGGCCCC CCGAACCACG    61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GAAAAAGCCT   121 GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC   181 CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT   241 GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTCAT   301 CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC   361 GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT   421 GAAACCGAAC TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG   481 GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC   541 ACTACGTGGC GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT   601 GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG   661 GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC   721 CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT   781 TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGANGCCGT GGTTGGCTTG TATGGAGCAG   841 CAGANGCGCT ACTTCGAGCG GANGCATCCG GANCTTGCAN GNNCGCCGCG GCTCCNGGCG   901 TATATGCTCC GCATTGGNCT NGANCNACTC TATCANANCT TGGNTGANGG CNNNTTCNAT   961 GANGCANCTT GGGNGCNNGN NCNNTGCGAC GCNNNNGNCC NNTNCNNNNN NCNNGNNNNN 1021 NNNGNNNNNN NNNN //
Primer: HygR-C_F (Pr0501) Region: HygR 3',P2A,EGFP 5' Sequence file: RDB12108_A3E7b.seq
>12108_12108_A3E7_1_HygR-C_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNGTCGC CNACATCTTC TTCTGGAGGC CGTGGTTGGC    61 TTGTATGGAG CNNCAGACGC GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC   121 GCGGCTCCGG GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA   181 CGGCAATTTC GATGATGCAG CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG   241 AGCCGGGACT GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG   301 CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA   361 GGAAATGCAT GCCACCAACT TCTCCCTGCT GAAGCAGGCC GGCGACGTGG AGGAGAACCC   421 CGGCCCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA   481 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC   541 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG   601 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA   661 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC   721 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA   781 CACCCTGGTG AACCGCATCG AGCTGANNGG CATCGACTTC AANGAGGANG GCAACATCCT   841 GGGGCACAAG CTGGANTACA ACTACNACAG CCACAACGTC TATATCATGG CCGANAAGCA   901 GAANNAACGG CATCNAGGNG AACNTCNAGN TNCGCNNCAA CATCNANGNA NGGNAGCNNG 961 CANCNCGCNN ANCACTACCA NCAGANNNCC CCCNNTCGGN NNNNNNCCNN NNNNNNNN //
Primer: pCAG-F_V3 (Pr0495) Region: BstXI stuffer,IRES Sequence file: RDB12108_A3E7c.seq
>12108_12108_A3E7_1_pCAG-F_V3_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNANNNCG GGGTNCGGCT TCTGGCGTGT    61 GANCGGNNNN NCNNNAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT   121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCCTCGAGAT   181 CCATTGTGCT GGCGGTAGGC GAGCAGCGCC TGCCTGAAGC TGCGGGCATT CCCGATCAGA   241 AATGAGCGCC AGTCGTCGTC GGCTCTCGGC ACCGAATGCG TATGATTCTC CGCCAGCATG   301 GCTTCGGCCA GTGCGTCGAG CAGCGCCCGC TTGTTCCTGA AGTGCCAGTA AAGCGCCGGC   361 TGCTGAACCC CCAACCGTTC CGCCAGTTTG CGTGTCGTCA GACCGTCTAC GCCGACCTCG   421 TTCAACAGGT CCAGGGCGGC ACGGATCACT GTATTCGGCT GCAACTTTGT CATGCTTGAC   481 ACTTTATCAC TGATAAACAT AATATGTCCA CCAACTTATC AGTGATAAAG AATCCGCGCC   541 AGCACAATGG ATCTCGAGGA ATTCACTCCT CAGGTGCAGG CTGCCTATCA GAAGGTGGTG   601 GCTGGTGTGG CCAACTGGCT CACAAATACC ACTGAGATCG ACGGTATCGA GCGGCCGCGG   661 TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC   721 TTCTTGANNA NNNNNNCTAG GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG   781 AATGTCGTGA ANGAAGCAGT TCCTCTGGAA GCTTCTTGAN NNCAAACAAC GTCTGTNNCG   841 ACCCTTNGCA NGCAGCGNAN NCCCCCCACC TGGNGACNGG TGCNTCTGCN NNCNAAANNC 901 NNCGNGNATN AAGANACACN TGCANAGGCG GNACNACCCC NNGNGCNNCN NNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: EGFP 3' Sequence file: RDB12108_A3E7d.seq
>12108_12108_A3E7_1_bGH_rev3_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNGNNNGGG GGNNGGGCAN CAACNGANGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGN TCAGCGAGCT CTAGATCATC GATTACTTGT ACAGCTCGTC CATGCCGAGA   121 GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG   181 TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG   241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG   301 TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG   361 ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG   421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG   481 GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT   541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG   601 CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG   661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTANGTGG CATCGCCCTC GCCCTCGCCG   721 GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC   781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGGGNNNG NGTTCTCCTC CACGTCNCCN   841 GCCTGCTTCA NCNNGNANAA GTTGGTGGCA TGCATTTCCT TTGCCNNCNN ANGANTGCTG   901 GGGGCGTCNN NTNNCACTAT CGGCNANTAC TTCTACACAG CCNTCNNNNC ANANNNCNNC   961 GCTTCTGNNG GCGANTNGNN NACNNNNNNN NNCCNNNNTN CNNNANNNGN NNNANNNNNN 1021 NNNN //
Primer: IRES-M_R (Pr0406) Region: BstXI stuffer Sequence file: RDB12108_A3E7e.seq
>12108_12108_A3E7_1_IRES-M_R_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTGC TTCNTTNNCG ACNTTNNACN GACCTTGCAT TCCTTTGGCG    61 AGAGGGGAAN NNNNCCCTAG GAATGCTCGT CAAGAAGACA GGGCCAGGTT TCCGGGCCCT   121 CACATTGCCA AAAGACGGCA ATATGGTGGA AAATAACCGC GGCCGCTCGA TACCGTCGAT   181 CTCANTGGTA TTTGTGAGCC AGTTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG   241 CACCTGAGGA GTGAATTCCT CGAGATCCAT TGTGCTGGCG CGGATTCTTT ATCACTGATA   301 AGTTGGTGGA CATATTATGT TTATCAGTGA TAAAGTGTCA AGCATGACAA AGTTGCAGCC   361 GAATACAGTG ATCCGTGCCG CCCTGGACCT GTTGAACGAG GTCGGCGTAG ACGGTCTGAC   421 GACACGCAAA CTGGCGGAAC GGTTGGGGGT TCAGCAGCCG GCGCTTTACT GGCACTTCAG   481 GAACAAGCGG GCGCTGCTCG ACGCACTGGC CGAAGCCATG CTGGCGGAGA ATCATACGCA   541 TTCGGTGCCG AGAGCCGACG ACGACTGGCG CTCATTTCTG ATCGGGAATG CCCGCAGCTT   601 CAGGCAGGCG CTGCTCGCCT ACCGCCAGCA CAATGGATCT CGAGGAATTC TTTGCNNNNN 661 NNATGANNNN NNACNNNNNN NNNN //
Primer: CAGen_R1 (Pr0616) Region: CAG pro,SV40 pA,polyoma ori Sequence file: RDB12108_A3E7f.seq
>12108_12108_A3E7_1_CAGen_R1_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNGGNCG GGGGTCGTTG GGCGGTCNGC CNGGCGGGCC ATTTACCGTA    61 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC   121 TATTAATAAC TAGTCAATAA TCAATGTCGA CGATGATATC AGATCCAGAC ATGATAAGAT   181 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG   241 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAATT   301 CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG TAATTTTCCT CCCGACTCTT   361 AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA ACTGACCGCA GCTGGCCGTG   421 CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC AGAGGGCAGT GTGGTTTTGC   481 AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT CAGTGTGGTT TTGCAAGAGG   541 AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC CCAATCATTA CTATGACAAC   601 AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGCCCCT GGCCTCCGCT TACTCTGGAG   661 AAAAAGAAGA GAGGCTTCCA GAGGCAACTT GTCAAAACAG GACTGGCGCC TTGGAGGCGC   721 TGTGGGGCCA CCCAAATTGA TATAATTAAG CCCCAACCGC CTCTTCCCGC CTCATTTCAG   781 CCTCACCACT ATGTGAATAT AGCTGANTAC ACAGTTTATT GAATAAACAT TAATTTCNAG   841 GAAATACAGT CTTTGTTTTT CCNAAGCGNN CATTGAAAAA GGAAGANTAT GANTATTCAA   901 CATTTCCGNG TCGCCCTTNN NCCCNTTTTT TGNGGNATTT TGCNNTCCNG NTTTTGCNCN 961 CCNNGAAANN CTNGNGAANN NNAANNNNGC TGNNNNNCAN TNGGNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,SV40 pA,ori Sequence file: RDB12108_A3E7g.seq
>12108_12108_A3E7_1_SV40pro_ori_F_G01_19_ABI24.ab1     1 NNNNNNNNNN GNNNNNGNNG CCTCGGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT AACTTGTTTA TTGCAGCTTA TAATGGTTAC   121 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT   181 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCCGCTG CATTAATGAA   241 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA   301 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG   361 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC   421 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC   481 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC   541 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC   601 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA   661 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC   721 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA   781 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAN   841 CGAGGTATGT ANGCGGTGCT ACAGANTTCT TGAAGTGGTG GNCTAACTAC NGCTACNCTA   901 NAAGAACAGT ANTTGGTATC TGCGCTCNNC TNNNNNCNGT TACCTTNNGA AAAAGANTNG   961 GTANCNNNNG NTNCNNNNAA NNAANNNNCN NNNNNNNNCN GNNNGNNTTT TTNNNTNNNN 1021 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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External Database
Aequorea victoria GFP

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