Mammalian expression vector. Blasticidin S resistance.
| Catalog number | RDB12107 |
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| Resource name | pPyCAG-BstXI-IB |
| Alternative name | no. 10007 |
| Clone info. | Mammalian expression vector. Blasticidin S resistance. |
| Vector backbone | Plasmid |
| Size of vector backbone | 6.1 kb |
| Selectable markers | Amp^r (E. coli), BSD^r (mammalian cells) |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB12107 | pPyCAG-BstXI-IB | DNA solution |
Electronic file
| Electronic file | Sequence RDB12107z.seq from Depositor |
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| Electronic file | Map RDB12107a.gif from Depositor
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12107_A5L9p1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB12107_A5L9a.seq |
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>RDB12107_12107_A5L9_pAxCA_F1_A10_01.ab1 1 CGGGGAAAAA GACGGGGTAC TTCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG 61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGATCCATT 121 GTGCTGGCGG TAGGCGAGCA GCGCCTGCCT GAAGCTGCGG GCATTCCCGA TCAGAAATGA 181 GCGCCAGTCG TCGTCGGCTC TCGGCACCGA ATGCGTATGA TTCTCCGCCA GCATGGCTTC 241 GGCCAGTGCG TCGAGCAGCG CCCGCTTGTT CCTGAAGTGC CAGTAAAGCG CCGGCTGCTG 301 AACCCCCAAC CGTTCCGCCA GTTTGCGTGT CGTCAGACCG TCTACGCCGA CCTCGTTCAA 361 CAGGTCCAGG GCGGCACGGA TCACTGTATT CGGCTGCAAC TTTGTCATGC TTGACACTTT 421 ATCACTGATA AACATAATAT GTCCACCAAC TTATCAGTGA TAAAGAATCC GCGCCAGCAC 481 AATGGATCTC GAGGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG 541 TGTGGCCAAC TGGCTCACAA ATACCACTGA GATCGACGGT ATCGAGCGGC CGCTCGATAA 601 GCTTGATATC GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAA CGTTACTGGC 661 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG 721 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCCTGGCCCT GTCTTCTTGA CGAGCATTCC 781 TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTTGAATGTC GTGAAGGAAG 841 CAGTTCCTCT GGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG 901 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGGCCAA AGCCACGTGT ATAAGATACA 961 CTTGCAAAGG CGGCACAACC CCCAGTGCCA CGTTGTGAGT TGGGATAGTT GTGGAAAAGA 1021 GTTCAAATGG CCTCTCCTCC AGCGTATTTC AACAGGGGGC TTGAAAGATG CCCAGAAAGG 1081 TACCCCATTG TATGGGATCT GATTCTGGGG GTCCTCAGTG CACATGCCTT TACATGGTGT 1141 TTTAGTCCGA GATAAAACCG TGTTCTAGGT CCTTCCTGAT CCCCGTGGGA CCGGTTGCTT 1201 TTTCTCTTTT TGTGAAAAAC GAC // |
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| Primer: IRES_F | RDB12107_A5L9b.seq |
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>RDB12107_12107_A5L9_IRES_F_B10_04.ab1 1 TGAAATCATT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG 61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGCC TTTGTCTCAA 121 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA 181 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT 241 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT 301 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG 361 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA 421 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT 481 TATGTGTGGG AGGGTTAACT AGATAAGTAA TGATCATAAT CAGCCATATC ACATCTGTAG 541 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 601 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 661 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 721 AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC 781 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 841 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 901 CACAGAATCA AGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 961 GAACCGTAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 1021 CACAAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 1081 CGTTTCCCCC TGAAGCTCCC TCGTGCGCCT CCTCCTGGTC GAACCCTGCG GCTACGGATA 1141 CCTGTTCCGC CTTCTCTCCC CTTCGGAAGC CGTGTGCCGC GCCCT // |
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| Primer: Amp_R | RDB12107_A5L9c.seq |
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>12107_12107_A9EkF_Amp_R_A10_01_ABI08.ab1 1 CTAAGGTGGC CCGGGCTTTG GGAAAACAAA GACTGTATTT CCTGGAAATT AATGTTTATT 61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA 121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT 181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC 241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG 301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT 361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC 421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG 481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT 541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA 601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC 721 TGATATCATC GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA 781 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT 841 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA 901 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC 961 TTGGCAGTAC ATCAAGTGTA TCATATGCCC AAGTACGCCC CCCTATTGAC GTCAATGACG 1021 GTAAATGGGC CCGCCTGGCA TTATGCCCAG TACATGACCC TTATGGACTT TCTTACTGGG 1081 CAGTACATCT ACGTATAGGT CAATCGCCTA TTACCCAATT GGGTCGAAGG GGTGAGCTCC 1141 CAACCGCGT // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.05.29
GNP_filter3_RDBDEP_html_190430.pl