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pPyCAG-BstXI-IB

Mammalian expression vector. Blasticidin S resistance.

Catalog number RDB12107
Resource name pPyCAG-BstXI-IB
Alternative name no. 10007
Clone info. Mammalian expression vector. Blasticidin S resistance.
Vector backbone Plasmid
Size of vector backbone 6.1 kb
Selectable markers Amp^r (E. coli), BSD^r (mammalian cells)
Depositor|Developer Niwa, Hitoshi |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB12107 pPyCAG-BstXI-IB DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB12107z.seq
            Remarks, protocol and/or map (gif) RDB12107a.gif

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12107_A5L9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB12107_A5L9a.seq check
            >RDB12107_12107_A5L9_pAxCA_F1_A10_01.ab1
                1 CGGGGAAAAA GACGGGGTAC TTCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
               61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGATCCATT
              121 GTGCTGGCGG TAGGCGAGCA GCGCCTGCCT GAAGCTGCGG GCATTCCCGA TCAGAAATGA
              181 GCGCCAGTCG TCGTCGGCTC TCGGCACCGA ATGCGTATGA TTCTCCGCCA GCATGGCTTC
              241 GGCCAGTGCG TCGAGCAGCG CCCGCTTGTT CCTGAAGTGC CAGTAAAGCG CCGGCTGCTG
              301 AACCCCCAAC CGTTCCGCCA GTTTGCGTGT CGTCAGACCG TCTACGCCGA CCTCGTTCAA
              361 CAGGTCCAGG GCGGCACGGA TCACTGTATT CGGCTGCAAC TTTGTCATGC TTGACACTTT
              421 ATCACTGATA AACATAATAT GTCCACCAAC TTATCAGTGA TAAAGAATCC GCGCCAGCAC
              481 AATGGATCTC GAGGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG
              541 TGTGGCCAAC TGGCTCACAA ATACCACTGA GATCGACGGT ATCGAGCGGC CGCTCGATAA
              601 GCTTGATATC GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAA CGTTACTGGC
              661 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG
              721 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCCTGGCCCT GTCTTCTTGA CGAGCATTCC
              781 TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTTGAATGTC GTGAAGGAAG
              841 CAGTTCCTCT GGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG
              901 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGGCCAA AGCCACGTGT ATAAGATACA
              961 CTTGCAAAGG CGGCACAACC CCCAGTGCCA CGTTGTGAGT TGGGATAGTT GTGGAAAAGA
             1021 GTTCAAATGG CCTCTCCTCC AGCGTATTTC AACAGGGGGC TTGAAAGATG CCCAGAAAGG
             1081 TACCCCATTG TATGGGATCT GATTCTGGGG GTCCTCAGTG CACATGCCTT TACATGGTGT
             1141 TTTAGTCCGA GATAAAACCG TGTTCTAGGT CCTTCCTGAT CCCCGTGGGA CCGGTTGCTT
            1201 TTTCTCTTTT TGTGAAAAAC GAC
            //
            Primer: IRES_F
            Sequence file: RDB12107_A5L9b.seq check
            >RDB12107_12107_A5L9_IRES_F_B10_04.ab1
                1 TGAAATCATT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
               61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGCC TTTGTCTCAA
              121 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
              181 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
              241 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
              301 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
              361 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
              421 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
              481 TATGTGTGGG AGGGTTAACT AGATAAGTAA TGATCATAAT CAGCCATATC ACATCTGTAG
              541 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
              601 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
              661 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
              721 AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC
              781 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
              841 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
              901 CACAGAATCA AGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
              961 GAACCGTAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
             1021 CACAAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
             1081 CGTTTCCCCC TGAAGCTCCC TCGTGCGCCT CCTCCTGGTC GAACCCTGCG GCTACGGATA
            1141 CCTGTTCCGC CTTCTCTCCC CTTCGGAAGC CGTGTGCCGC GCCCT
            //
            Primer: Amp_R
            Sequence file: RDB12107_A5L9c.seq check
            >12107_12107_A9EkF_Amp_R_A10_01_ABI08.ab1
                1 CTAAGGTGGC CCGGGCTTTG GGAAAACAAA GACTGTATTT CCTGGAAATT AATGTTTATT
               61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
              121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
              181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
              241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
              301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
              361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
              421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
              481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT
              541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA
              601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
              661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC
              721 TGATATCATC GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA
              781 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
              841 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
              901 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
              961 TTGGCAGTAC ATCAAGTGTA TCATATGCCC AAGTACGCCC CCCTATTGAC GTCAATGACG
             1021 GTAAATGGGC CCGCCTGGCA TTATGCCCAG TACATGACCC TTATGGACTT TCTTACTGGG
             1081 CAGTACATCT ACGTATAGGT CAATCGCCTA TTACCCAATT GGGTCGAAGG GGTGAGCTCC
            1141 CAACCGCGT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user report Tani, R., Linker histone variant H1T targets rDNA repeats. Epigenetics 11 (4): 288-302 (2016). PMID 27018843.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl