Resource data sheet
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pPyCAG-BstXI-IB

Mammalian expression vector. Blasticidin S resistance.

Catalog number RDB12107
Resource name pPyCAG-BstXI-IB
Alternative name no. 10007
Clone info. Mammalian expression vector. Blasticidin S resistance.
Vector backbone Plasmid
Size of vector backbone 6.1 kb
Selectable markers Amp^r (E. coli), BSD^r (mammalian cells)
Depositor Niwa, Hitoshi |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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RDB12107 pPyCAG-BstXI-IB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12107z.seq from Depositor
Electronic file Map RDB12107a.gif from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12107_A5L9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB12107_A5L9a.seq
>RDB12107_12107_A5L9_pAxCA_F1_A10_01.ab1
    1 CGGGGAAAAA GACGGGGTAC TTCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGATCCATT
  121 GTGCTGGCGG TAGGCGAGCA GCGCCTGCCT GAAGCTGCGG GCATTCCCGA TCAGAAATGA
  181 GCGCCAGTCG TCGTCGGCTC TCGGCACCGA ATGCGTATGA TTCTCCGCCA GCATGGCTTC
  241 GGCCAGTGCG TCGAGCAGCG CCCGCTTGTT CCTGAAGTGC CAGTAAAGCG CCGGCTGCTG
  301 AACCCCCAAC CGTTCCGCCA GTTTGCGTGT CGTCAGACCG TCTACGCCGA CCTCGTTCAA
  361 CAGGTCCAGG GCGGCACGGA TCACTGTATT CGGCTGCAAC TTTGTCATGC TTGACACTTT
  421 ATCACTGATA AACATAATAT GTCCACCAAC TTATCAGTGA TAAAGAATCC GCGCCAGCAC
  481 AATGGATCTC GAGGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG
  541 TGTGGCCAAC TGGCTCACAA ATACCACTGA GATCGACGGT ATCGAGCGGC CGCTCGATAA
  601 GCTTGATATC GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAA CGTTACTGGC
  661 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG
  721 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCCTGGCCCT GTCTTCTTGA CGAGCATTCC
  781 TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTTGAATGTC GTGAAGGAAG
  841 CAGTTCCTCT GGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG
  901 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGGCCAA AGCCACGTGT ATAAGATACA
  961 CTTGCAAAGG CGGCACAACC CCCAGTGCCA CGTTGTGAGT TGGGATAGTT GTGGAAAAGA
 1021 GTTCAAATGG CCTCTCCTCC AGCGTATTTC AACAGGGGGC TTGAAAGATG CCCAGAAAGG
 1081 TACCCCATTG TATGGGATCT GATTCTGGGG GTCCTCAGTG CACATGCCTT TACATGGTGT
 1141 TTTAGTCCGA GATAAAACCG TGTTCTAGGT CCTTCCTGAT CCCCGTGGGA CCGGTTGCTT
1201 TTTCTCTTTT TGTGAAAAAC GAC
//
Primer: IRES_F RDB12107_A5L9b.seq
>RDB12107_12107_A5L9_IRES_F_B10_04.ab1
    1 TGAAATCATT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGCC TTTGTCTCAA
  121 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
  181 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
  241 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
  301 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
  361 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
  421 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
  481 TATGTGTGGG AGGGTTAACT AGATAAGTAA TGATCATAAT CAGCCATATC ACATCTGTAG
  541 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA
  601 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  661 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  721 AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC
  781 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
  841 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
  901 CACAGAATCA AGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
  961 GAACCGTAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
 1021 CACAAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
 1081 CGTTTCCCCC TGAAGCTCCC TCGTGCGCCT CCTCCTGGTC GAACCCTGCG GCTACGGATA
1141 CCTGTTCCGC CTTCTCTCCC CTTCGGAAGC CGTGTGCCGC GCCCT
//
Primer: Amp_R RDB12107_A5L9c.seq
>12107_12107_A9EkF_Amp_R_A10_01_ABI08.ab1
    1 CTAAGGTGGC CCGGGCTTTG GGAAAACAAA GACTGTATTT CCTGGAAATT AATGTTTATT
   61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA
  121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT
  181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC
  241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG
  301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT
  361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC
  421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG
  481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT
  541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA
  601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC
  721 TGATATCATC GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA
  781 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
  841 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
  901 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
  961 TTGGCAGTAC ATCAAGTGTA TCATATGCCC AAGTACGCCC CCCTATTGAC GTCAATGACG
 1021 GTAAATGGGC CCGCCTGGCA TTATGCCCAG TACATGACCC TTATGGACTT TCTTACTGGG
 1081 CAGTACATCT ACGTATAGGT CAATCGCCTA TTACCCAATT GGGTCGAAGG GGTGAGCTCC
1141 CAACCGCGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.05.29

GNP_filter3_RDBDEP_html_190430.pl