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Mammalian expression vector. Blasticidin S resistance.
| Catalog number | RDB12107 |
|---|---|
| Resource name | pPyCAG-BstXI-IB |
| Alternative name | no. 10007 |
| Clone info. | Mammalian expression vector. Blasticidin S resistance. |
| Vector backbone | Plasmid |
| Size of vector backbone | 6.1 kb |
| Selectable markers | Amp^r (E. coli), BSD^r (mammalian cells) |
| Depositor|Developer | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. |
|---|---|
| Additional terms and conditions for distribution | Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested. |
| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA) [Word ] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB12107 | pPyCAG-BstXI-IB | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Sequence RDB12107z.seq |
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| Electronic file | Map RDB12107a.gif
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12107_A5L9p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pAxCA_F1 Sequence file: RDB12107_A5L9a.seq
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>RDB12107_12107_A5L9_pAxCA_F1_A10_01.ab1 1 CGGGGAAAAA GACGGGGTAC TTCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG 61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGATCCATT 121 GTGCTGGCGG TAGGCGAGCA GCGCCTGCCT GAAGCTGCGG GCATTCCCGA TCAGAAATGA 181 GCGCCAGTCG TCGTCGGCTC TCGGCACCGA ATGCGTATGA TTCTCCGCCA GCATGGCTTC 241 GGCCAGTGCG TCGAGCAGCG CCCGCTTGTT CCTGAAGTGC CAGTAAAGCG CCGGCTGCTG 301 AACCCCCAAC CGTTCCGCCA GTTTGCGTGT CGTCAGACCG TCTACGCCGA CCTCGTTCAA 361 CAGGTCCAGG GCGGCACGGA TCACTGTATT CGGCTGCAAC TTTGTCATGC TTGACACTTT 421 ATCACTGATA AACATAATAT GTCCACCAAC TTATCAGTGA TAAAGAATCC GCGCCAGCAC 481 AATGGATCTC GAGGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG 541 TGTGGCCAAC TGGCTCACAA ATACCACTGA GATCGACGGT ATCGAGCGGC CGCTCGATAA 601 GCTTGATATC GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAA CGTTACTGGC 661 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG 721 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCCTGGCCCT GTCTTCTTGA CGAGCATTCC 781 TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTTGAATGTC GTGAAGGAAG 841 CAGTTCCTCT GGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG 901 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGGCCAA AGCCACGTGT ATAAGATACA 961 CTTGCAAAGG CGGCACAACC CCCAGTGCCA CGTTGTGAGT TGGGATAGTT GTGGAAAAGA 1021 GTTCAAATGG CCTCTCCTCC AGCGTATTTC AACAGGGGGC TTGAAAGATG CCCAGAAAGG 1081 TACCCCATTG TATGGGATCT GATTCTGGGG GTCCTCAGTG CACATGCCTT TACATGGTGT 1141 TTTAGTCCGA GATAAAACCG TGTTCTAGGT CCTTCCTGAT CCCCGTGGGA CCGGTTGCTT 1201 TTTCTCTTTT TGTGAAAAAC GAC // |
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Primer: IRES_F Sequence file: RDB12107_A5L9b.seq
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>RDB12107_12107_A5L9_IRES_F_B10_04.ab1 1 TGAAATCATT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG 61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGCC TTTGTCTCAA 121 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA 181 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT 241 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT 301 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG 361 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA 421 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT 481 TATGTGTGGG AGGGTTAACT AGATAAGTAA TGATCATAAT CAGCCATATC ACATCTGTAG 541 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 601 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 661 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 721 AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC 781 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 841 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 901 CACAGAATCA AGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 961 GAACCGTAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 1021 CACAAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 1081 CGTTTCCCCC TGAAGCTCCC TCGTGCGCCT CCTCCTGGTC GAACCCTGCG GCTACGGATA 1141 CCTGTTCCGC CTTCTCTCCC CTTCGGAAGC CGTGTGCCGC GCCCT // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.10.02
GNP_filter3_RDBDEP_html_200316.pl