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pBRPyCAG-Klf5(2)-IP

Expression clone of mouse Klf5

Catalog number RDB10857
Resource name pBRPyCAG-Klf5(2)-IP
Alternative name stock no 1872
Clone info. Expression clone of mouse Klf5. cDNA fragment 365nt to 1645nt of NM_009769.3 is cloned.
Known difference: G to A substitution at 376nt (synoym).
Vector backbone pBRPyCAG-cHA-IP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Klf5 cDNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank mouse Klf5 (NCBI Gene 12224) |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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RDB10857 pBRPyCAG-Klf5(2)-IP DNA solution

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References and tips

Electronic file

Electronic file Sequence RDB10857z.seq from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB10857_A8Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB10857_A8Eta.seq
>10857_10857_A8Et_4_pAxCA_F1_A08_02_ABI24.ab1
    1 GGCTGCTACA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA
   61 TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCCTCGAGAT CCAATCCCCA GACCGTTCAT
  121 GCCCACGCGG GTGCTGACCA TGAGCGCCCG CCTGGGACCA CTGCCCCAGC CGCCGGCCGC
  181 GCAGGACGAA CCCGTGTTCG CGCAGCTCAA GCCGGTGCTG GGCGCTGCGA ACCCGGCCCG
  241 CGACGCGGCG CTCTTCTCCG GAGACGATCT GAAACACGCG CACCACCACC CGCCTGCGCC
  301 GCCGCCAGCC GCTGGCCCGC GACTGCCCTC GGAGGAGCTG GTCCAGACAA GATGTGAAAT
  361 GGAGAAGTAT CTGACCCCTC AGCTCCCTCC AGTTCCGATA ATTTCAGAGC ATAAAAAGTA
  421 TAGACGAGAC AGTGCCTCAG TGGTAGACCA GTTCTTCACT GACACTGAAG GCATACCTTA
  481 CAGCATCAAC ATGAACGTCT TCCTCCCTGA CATCACTCAC CTGAGAACTG GCCTCTACAA
  541 ATCCCAGAGA CCATGCGTAA CACAGATCAA GACAGAACCT GTTACCATTT TCAGCCACCA
  601 GAGCGAGTCG ACGGCCCCTC CTCCTCCTCC GGCCCCCACC CAGGCTCTCC CCGAGTTCAC
  661 TAGTATCTTC AGCTCCCACC AGACCACAGC GCCACCACAG GAGGTGAACA ATATCTTCAT
  721 CAAACAAGAA CTTTCCTAAT ACCAGATCTT CATCTCTCTG TCCCTTTCCC AGCAGGCCAC
  781 CTGTACCAGC TGTTGAATAC ACCGGAATCT AGACATGCCC AGGTTCGAAC AAACCAGACG
  841 GGCAGTTATT GGACACCCTT AAATGTTTTC TTATTGGGCA GGCTTAACCC AACACCCCCT
  901 TCTGCTGTTC ACAGAACGTT CATGAAACAG TTCAAGGCAA TGCTCCCTTT GGCACGTACA
  961 CCATGGCAAG TTCAGTTCTT CCACAAGCAG CACCTACTTC CCCGTACTGC CAGCCAGATC
1021 CTGAAAGTTC CTG
//
Primer: CSII-CMV3' RDB10857_A8Etb.seq
>10857_10857_A8Et_4_CSII-CMV3'_F12_18_ABI24.ab1
    1 CGGGGGTCAT TCGATATCAA GCTTATCGAG CGGCCCGCTC AGTTCTGGTG GCGCTTCATG
   61 TGCAGCGCGA GGTGGTCGGA GCGGGAGAAG CTGCGTTGGC ACACCATGCA CTGGAACGGC
  121 TTGGCGCCCG TGTGCTTCCT GTAGTGGCGG GTCAGCTCAT CCGACCGGGC AAACCTCCAG
  181 TCGCAGCCCT CCCAGGTGCA CTTGTAGGGC TTCTCGCCCG TATGAGTCCT CAGGTGAGCT
  241 TTTAAGTGAG ACGACTTTGT ATAAACTTTT GTGCAACCAT TATAATCGCA GAAGTGGATA
  301 CGTCGCTTCT CCAGATCCGG GTTACTCCTT CTGTTGTATC TGACAGGTGG GAGAGTTGGC
  361 GAATTAACTG GCAGAGTGGC AGGTAAATTT GGGTTGTGAA TCGCCAGTTT GGAAGCAATT
  421 GTAGCGGCAT AGGACGGAGG TGGGGTGAGA TTCTGCAGCA TCTCAGCTTG TCTATCGGGA
  481 CTTCCAGGCT CTGAGCTTGG TGGTGACGGG GGAAAGTAGG TGGCCTGCTG TGGAAGAAAC
  541 TGACTTGGCA TGGTGTACGT GCAAGGGGGC ATGCCCTGGA ACTGTTTCAT TGACGTCTGT
  601 GGAACAGCAG AGGGGTGTGG GTTAAGGCCT GCCATAGAAA CATTAAGGGT GTCCATTACT
  661 GCCGTCTGGT TTGTCGAACT GGGCATGTCT AGATCCGGTG TATTCAACAG CTGGTACAGG
  721 TGGCCCTGCT GGGAAGGGAC AGAGAGATGA AGATCTGGTA TAGGAAGTTC TTGTTTGATG
  781 AAGATATTGT TCACCTCCTG TGGTGGCGCT GTGGTCTGGT GGGAGCTGAA GATACTAGTG
  841 AACTCGGGGG AGAGCCTGGG TGGGGGGCCG GAGGAGGAGG AGGGGGCCGT CGACTCGCTC
  901 TGGTGGCTGA AAATGGGTAA CAGGTTCTGT CTTGATCTGT GTTACGCATG GTCTCTGGGA
  961 TTTGTAGAGG CCAGGTTCTC AGGTGAGTGA TGTTCAAGGA GGAGACGTTC ATGTTTGAAT
 1021 GCTGTAATGT AATGCCCTTC CAGTGTCAGT GGAAGAACTT GGCTCTTACC CACTTGGGAG
1081 GGCCACCC
//
Primer: CMV_en_R RDB10857_A8Etc.seq
>10857_10857_A8Et_4_CMV_en_R_C08_08_ABI24.ab1
    1 TTTATTAATT GATTACTATT AATAACTAGC TAGTCAATAA TCAATGTCGA CGATGATATC
   61 AGATCCAGAT ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
  121 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
  181 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG
  241 TAATTTTCCT CCCGACTCTT AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA
  301 ACTGACCGCA GCTGGCCGTG CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC
  361 AGAGGGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT
  421 CAGTGTGGTT TTGCAAGAGG AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC
  481 CCAATCATTA CTATGACAAC AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGGCCCC
  541 TGGCCTCCGC TTACTCTGGA GAAAAAGAAG AGAGGCTTCC AGAGGCAACT TGTCAAAACA
  601 GGACTGGCGC CTTGGAGGCG CTGTGGGGCC ACCCAAATTG ATATAATTAA GCCCCAACCG
  661 CCTCTTCCCG CCTCATTTCA GCCTCACACT ATGTGAAATA ATAGCTGAAT ACACAGTTTA
  721 TTGAATAAAA CATTAATTTC CAGGAAAATA ACAAGTCTTT GTTTTTCCAA AGCGTCATTG
  781 AAAAGAAAGA AGTATGAGTA TCAACATTCC GTGTCGCCTA ATCCTTTTTT GCGCAATTGC
  841 CTCTGTTTGC TCACCAGAAC GCTGTGAAAG TTAAAAGAAT GCTGAAAGAA TACGATTGTG
  901 CACAGATTGG TACATCGACT GAATTCCACA CGGTTAGAGT CTGGAAGTTC GCTCCGAAGA
961 A
//
Primer: SV40pro_R RDB10857_A8Etd.seq
>10857_10857_A8Et_4_SV40pro_R_D08_11_ABI24.ab1
    1 GCAATCGGAA AAACGGAGAA TGGGCGGAAC TGGGCGGAGT TAGCGCGGCG GGATGGGCGG
   61 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC GGATCCGCTG GCACGACAGG
  121 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
  181 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
  241 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGGGCTGCAG
  301 GTTCTTTCCG CCTCAGAAGC CATAGAGCCC ACCGCATCCC CAGCATGCCT GCTATTGTCT
  361 TCCCAATCCT CCCCCTTGCT GTCCTGCCCC ACCCCACCCC CCAGAATAGA ATGACACCTA
  421 CTCAGACAAT GCGATGCAAT TTCCTCATTT TATTAGGAAA GGACAGTGGG AGTGGCACCT
  481 TCCAGGGTCA AGGAAGGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
  541 ACAGTCGAGG CTGATCAGCG AGCTCTAGAT CATCGATGCA TGGGGTCGTG CGCTCCTTTC
  601 GGTCGGGCGC TGCGGGTCGT GGGGGCGGGC GTCAGGCACC GGGGCTTGCG GGTCATGCAC
  661 CAGGTGCGCG GGTCCTTTCG GGGCACCTCG ACGTCGGCGG GTGACGGTGA AACCCGAGCC
  721 GCTCGTAGAA GGGGGAGGGT TTGCGGGGGG GCGGAAGGTC TCCAGGAAAA GGCGGGGCAC
  781 CCCCGGCGCG CCTCGGCCCG CCCTCCCACT CCGGGGGGAA GCCCGACGGG CGCCTGCCCA
841 AAACCCTTGC CCCTGGGTGG GTCGGGGCGA AAACGCCTGA ACGG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.11

GNP_filter3_RDBDEP_html_181012.pl