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pBRPyCAG-Klf9(2)-IP

Expression clone of mouse Klf9

Catalog number RDB10847
Resource name pBRPyCAG-Klf9(2)-IP
Alternative name stock no 1861
Clone info. Expression clone of mouse Klf9.
Vector backbone pBRPyCAG-cHA-IP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Klf9 cDNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank mouse Klf9 (NCBI Gene 16601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB10847 pBRPyCAG-Klf9(2)-IP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB10847z.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10847_A8Etp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB10847_A8Eta.seq check
>10847_10847_A8Et_2_pAxCA_F1_C09_09_ABI24.ab1
    1 GGTTTGTCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG ATCTGCCGTC GCCCGCACCA
  121 TGTCCGCGGC CGCCTACATG GACTTCGTGG CTGCCCAGTG TCTGGTTTCC ATCTCCAACC
  181 GCGCCGCCGT GCCGGAGCAC GGGGGCGCTC CGGAAGCCGA GCGGCTGCGA CTACCTGAGC
  241 GCGAGGTGAC CAAGGAACAC GGTGACCCGG GGGACACCTG GAAGGATTAT TGCACGCTGG
  301 TCACTATCGC CAAGAGCTTG TTGGACCTCA ACAAATACCG ACCCATCCAG ACCCCCTCGG
  361 TGTGCAGCGA CAGTCTGGAG AGTCCCGATG AGGATATAGG ATCCGACAGC GACGTGACCA
  421 CCGAATCTGG GTCGAGTCCT TCCCACAGCC CGGAGGAGAG ACAGGATTCT GGCAGCGCGC
  481 CCAGCCCACT CTCCCTCCTC CACTCTGGAG TGGCTTCGAA GGGGAAACAC GCCTCCGAAA
  541 AGAGGCACAA GTGCCCCTAC AGTGGCTGTG GGAAAGTCTA TGGAAAATCC TCCCATCTTA
  601 AAGCCCATTA CAGAGTGCAT ACAGGTGAAC GGCCCTTTCC CTGCACGTGG CCAGACTGCC
  661 TTAAAAAGTT CTCGCGCTCG GATGAGCTGA CCCGCCACTA CCGGACCCAC ACTGGGGAAA
  721 AGCAGTTCCG TTGCCCACTG TGTGAGAAGA GATTCATGAG GAGTGACCAT CTCACCAAGC
  781 ATGCCCGGCG TCACACCGAG TTCCATCCCA GCATGATCAA GAGATCAAAA AAGGCTCTTG
  841 CCAGCCCCTT GTGAGGTTGA CCGGTGCGGC CGCTCGATAA GCTTGATATC GAATTCCGCC
  901 CCCCCCCCCC CTCTCCCTCC CCCCCCCCCC CTAACGTTAC TGGGCCGAAA GCCCGCTTTG
  961 GAATAAAGGC CGGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCCGTCTTTT
 1021 GGCATGGTGA GGACCCGGGA ACCTGGACCT GTCCTCCATG GACGAAGCCA TTCCTTAGGG
1081 ATCT
//
Primer: CSII-CMV3'
Sequence file: RDB10847_A8Etb.seq check
>10847_10847_A8Et_2_CSII-CMV3'_B06_06_ABI24.ab1
    1 CGGGGGAAAT TCGGATATCA AGCTTATCGA GCGGCCGCAC CGGTCAACCT CACAAGGGGC
   61 TGGCAAGAGC CTTTTTTGAT CTCTTGATCA TGCTGGGATG GAACTCGGTG TGACGCCGGG
  121 CATGCTTGGT GAGATGGTCA CTCCTCATGA ATCTCTTCTC ACACAGTGGG CAACGGAACT
  181 GCTTTTCCCC AGTGTGGGTC CGGTAGTGGC GGGTCAGCTC ATCCGAGCGC GAGAACTTTT
  241 TAAGGCAGTC TGGCCACGTG CAGGGAAAGG GCCGTTCACC TGTATGCACT CTGTAATGGG
  301 CTTTAAGATG GGAGGATTTT CCATAGACTT TCCCACAGCC ACTGTAGGGG CACTTGTGCC
  361 TCTTTTCGGA GGCGTGTTTC CCCTTCGAAG CCACTCCAGA GTGGAGGAGG GAGAGTGGGC
  421 TGGGCGCGCT GCCAGAATCC TGTCTCTCCT CCGGGCTGTG GGAAGGACTC GACCCAGATT
  481 CGGTGGTCAC GTCGCTGTCG GATCCTATAT CCTCATCGGG ACTCTCCAGA CTGTCGCTGC
  541 ACACCGAGGG GGTCTGGATG GGTCGGTATT TGTTGAGGTC CAACAAGCTC TTGGCGATAG
  601 TGACCAGCGT GCAATAATCC TTCCAGGTGT CCCCCGGGTC ACCGTGTTCC TTGGTCACCT
  661 CGCGCTCAGG TAGTCGCAGC CGCTCGGCTT CCGGAGCGCC CCCGTGCTCC GGCACGGCGG
  721 CGCGGTTGGA GATGGAAACC AGACACTGGG CAGCCACGAA GTCCATGTAG GCGGCCGCGG
  781 ACATGGTGCG GGCGACGGCA GATCTCGAGG AATTCTTTGC CAAAATGATG AGACAGCACA
  841 ATAACCAGCA CGTTTGCCCA GGAGCTGTAG GAAAAAGAAG AAGGGCATGA ACATGGTTAG
  901 CAGAGGCTCT AGAGCCGCCG GGTCACACGC CAGAAGCCGA ACCCCGGCCC TGGCCCCGGT
  961 CCCCCCCGAA AGGCAGCCCG TTCCCCCCGG CGGGACAGCC CCCGAAGCTG GAAGGGAGAA
 1021 GAACGGCCGA TCGCGGCGAA CGCACGAGGC CATCCCCGGC AATTTCTTTC CTGGCTGGCG
1081 CCCGACAACC CGTCT
//
Primer: CMV_en_R
Sequence file: RDB10847_A8Etc.seq check
>10847_10847_A8Et_2_CMV_en_R_C06_09_ABI24.ab1
    1 TGGGTTAATG ATTACTATTA ATAACTAGCT AGTCAATAAT CAATGTCGAC GATGATATCA
   61 GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
  121 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
  181 TGCAATAAAC AAGTTAATTC GATTCAGCTT CAGAAGATGG CGGAGGGCCT CCAACACAGT
  241 AATTTTCCTC CCGACTCTTA AAATAGAAAA TGTCAAGTCA GTTAAGCAGG AAGTGACTAA
  301 CTGACCGCAG CTGGCCGTGC GACATCCTCT TTTAATTAGT TGCTAGGCAA CTGCCCTCCA
  361 GAGGGCAGTG TGGTTTTGCA AGAGGAAGCA AAAAGCCTCT CCACCCAGGC CTGGAATGTC
  421 AGTGTGGTTT TGCAAGAGGA AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTTTCCACC
  481 CAATCATTAC TATGACAACA GCTGTTTTTT TTAGTATTAA GCAGAGGCCG GGGGCCCCTG
  541 GCCTCCGCTT ACTCTGGAGA AAAAGAAGAG AGGCTTCCAG AGGCAACTTG TCAAAACAGG
  601 ACTGGCGCCT TGGAGGCGCT GTGGGGCCAC CCAAATTGAT ATAATTAAGC CCCAACCGCC
  661 TCTTCCCGCC TCATTTCAGC CTCACCACTA TGTGAATATA GCTGAATACA CAGTTTATTG
  721 AATAAACATT AATTTCCAGG AAATACAGTC TTTGTTTTTC CAAAGCGGTC ATTGAAAAAG
  781 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG
  841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT
  901 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
  961 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTTAA AGTTCTGCTA TGTTGGCGCG
 1021 GTATAATCCC CGTATTGGAC GGTGGCAGAA GCACTCGTCG CCGCATACAC TATTCTCAGA
1081 ATGGAGTTGG GTGA
//
Primer: BGH_rev2
Sequence file: RDB10847_A8Etd.seq check
>10847_10847_A8Et_2_BGH_rev2_D06_12_ABI24.ab1
    1 CACATTTAAG GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGATCATCG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG
  121 GGCGCTGCGG GTCGTGGGGC GGGCGTCAGG CACCGGGCTT GCGGGTCATG CACCAGGTGC
  181 GCGGTCCTTC GGGCACCTCG ACGTCGGCGG TGACGGTGAA GCCGAGCCGC TCGTAGAAGG
  241 GGAGGTTGCG GGGCGCGGAG GTCTCCAGGA AGGCGGGCAC CCCGGCGCGC TCGGCCGCCT
  301 CCACTCCGGG GAGCACGACG GCGCTGCCCA GACCCTTGCC CTGGTGGTCG GGCGAGACGC
  361 CGACGGTGGC CAGGAACCAC GCGGGCTCCT TGGGCCGGTG CGGCGCCAGG AGGCCTTCCA
  421 TCTGTTGCTG CGCGGCCAGC CGGGAACCGC TCAACTCGGC CATGCGCGGG CCGATCTCGG
  481 CGAACACCGC CCCCGCTTCG ACGCTCTCCG GCGTGGTCCA GACCGCCACC GCGGCGCCGT
  541 CGTCCGCGAC CCACACCTTG CCGATGTCGA GCCCGACGCG CGTGAGGAAG AGTTCTTGCA
  601 GCTCGGTGAC CCGCTCGATG TGGCGGTCCG GATCGACGGT GTGGCGCGTG GCGGGGTAGT
  661 CGGCGAACGC GGCGGCGAGG GTGCGTACGG CCCTGGGGAC GTCGTCGCGG GTGGCGAGGC
  721 GCACCGTGGG CTTGTACTCG GTCATGGTTG TGGCCATATT ATCATCGTGT TTTTCAAAGG
  781 AAAACCACGT CCCCGTGGTT CGGGGGGCCT AGACGTTTTT TTAACCTCGA CTAAACACAT
  841 GTAAAGCATG TGCACCGAGG CCCCAGATCA GATCCCATAC AATGGGGGTA CCTTCTGGGC
  901 ATCCTTCAGC CCCCTTGTTG AAATACGCTT GAGGAGAGCC ATTTTGACTC TTTCCCACAA
  961 CTATCCAACT CACAACGTGG CACTGGGGGT TTGTGCCGCC CTTTGCAGGT GTATCTTTAT
 1021 ACACGTGGCT TTTTGCTCGC AGAGCACCTG TCGCAGCTTG GGGGGAATTC CGGCTGCCTG
1081 CAAAGGGATT CGGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

GNP_filter3_RDBDEP_html_200316.pl