Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-Klf2(1)-IP

Expression clone of mouse Klf2

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006)。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB10842_A7Cfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB10842_A7Cfa.seq
>10842_10842_A7Cf_pAxCA_F1_E05_14_ABI24.ab1     1 GAGTCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT    61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG ATCTAGTCCC TGCCATGGCG   121 CTCAGCGAGC CTATCTTGCC GTCCTTTGCC ACTTTCGCCA GCCCGTGCGA GCGCGGCCTC   181 CAGGAGCGCT GGCCGCGAAA TGAACCCGAG GCGGGCGGCA CGGATGAGGA CCTAAACAAC   241 GTGTTGGACT TCATCCTCTC CATGGGATTG GACGGTCTGG GCGCCGAAAA TCCTCCCGAG   301 CCCCCGCCGC AGCCCCCGCC GCCTGCCTTC TACTACCCGG AGCCGGGTGC TCCGCCGCCC   361 TACAGCATCC CCGCGGCCAG CCTGGGAACA GAGCTGCTGC GCCCCGACCT GGACCCGCCT   421 CAGGGGCCGG CTCTGCACGG CCGCTTCCTC CTCGCGCCTC CCGGGGCGGC TAGTGAAGGC   481 CGAGCCCCCC GAAGGTTGGA CGGGGGGCGG CTACGGCTGG GCTCCGGGGC CTGGGCCCAC   541 GGACCGCGGC GGGGCTGAAG CTCGAAGGGG CGGCCCCCAG GGAGCCGAAC AGGGGTGCAT   601 TGCATGCGGG GTTCCCGCCG GGCGGCCCCC GGCGGCCCTG GGAACACGCC GCCGCTTCAA   661 GCCCCGAACC GGCCCCTTGG GATTCCCGGC AATCCGGGTC CCCGCAAACC CGGTTTCCCG   721 GCGGCCTTCG GTCCCGGGCC CAGCTTTCGG CGGGTCCGGG CCCGCGGTTG CACTTCGGGG   781 CTTCCCGCGG CCTTGGGGGC TTTCGGGTCT TTCAAGGAAC GGCGGCAGCG GCGGGGGGGG   841 CCTTGTGGCT TTTGTTTTCA ACTGTGCCAA GGGGGGTTTC TTAACAGCGC GCCTTTGTTT   901 CGCTTGAGAA CTTGCTGTGA AGAGGAAAAG CTATACGCGG GGCCGCGTTC TTGTGTTCCG   961 GAAGAGAGCC GGCCCACCAC AATATTTGTG GCGCATACCA ACTTGAGGGA AGACTATCAC 1021 AATAAGAAAG A //
Primer: mKlf2-M_F Sequence file: RDB10842_A7Cfb.seq
>10842_10842_A7Cf_1_mKlf2-M_F_A05_02_ABI24.ab1     1 CTCAGTACGC CTCAGGCCGG CTCTGCACGG CCGCTTCCTC CTCGCGCCTC CCGGGCGGCT    61 AGTGAAGGCC GAGCCCCCCG AGGTGGACGG CGGCGGCTAC GGCTGCGCTC CGGGCCTGGC   121 CCACGGACCG CGCGGGCTGA AGCTCGAGGG CGCCCCAGGA GCGACAGGTG CATGCATGCG   181 GGGTCCCGCC GGCCGCCCCC CGCCGCCCCC GGACACGCCG CCGCTCAGCC CCGACGGCCC   241 CCTGCGCATC CCGGCATCCG GTCCCCGCAA CCCGTTCCCG CCGCCCTTCG GTCCCGGCCC   301 CAGCTTCGGC GGTCCCGGCC CCGCGTTGCA CTACGGGCCT CCCGCGCCTG GCGCCTTCGG   361 TCTTTTCGAG GACGCGGCGG CAGCGGCGGC GGCGCTGGGC TTGGCTCCAC CTGCCACGCG   421 CGGTCTCCTC ACGCCGCCCT CGTCCCCGCT GGAGCTGCTG GAGGCCAAGC CCAAACGCGG   481 CCGCCGCTCC TGGCCCCGCA AGCGCGCCGC CACACATACT TGCAGCTACA CCAACTGCGG   541 CAAGACCTAC ACCAAGAGCT CGCACCTAAA GGCGCATCTG CGTACACACA CAGGTGAGAA   601 GCCTTATCAT TGCAACTGGG AAGGATGCGG CTGGAAGTTC GCGCGCTCTG ACGAGCTTAC   661 CCGCCACTAC CGAAAGCACA CAGGTCACAG ACCCTTTCAG TGCCACTTGT GCGACCGGGC   721 CTTCTCCAGG TCCGACCACC TGGCCTTGCA CATGAAGCGA CACATGTAGC CTGTGACCGG 781 TGCGGCCGCT CGATAAGCTT GATATCGAAT TCCGCCCCCC CCCCCCTCTC CC //
Primer: IRES-F Sequence file: RDB10842_A7Cfc.seq
>10842_10842_A7Cf_1_IRES-F_C11_08_ABI24.ab1     1 TGCATCATTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA    61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC   121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG   181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG   241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG   301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG   361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC   421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG   481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC   541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC   601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG   661 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC   721 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATCTAG AGCTCGCTGA   781 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT   841 TCCTTGACCC TGGAAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC   901 ATCGCATTGT CTGAGTAGTG TCATTCTATT CCTGGGGGGT GGGGGTGGGG CAGACAGCAG   961 GGGAGGAAAT TGGGGAAAGA ACAAAAAGCA GGCATGCTGG GGATGCCGGT GGGCTTCTTT  1021 ATGGCTTCTG AGGCGGAAAG GAACTGCAGC CAAGCTGACG TATTCATGTC ATAGCTGATT 1081 CCTGGTTGAA ATGGATATCC GGCCTCACAA AGTTCGCCAC CAAGCGAT //
Primer: SV40pA3'Element Reverse Sequence file: RDB10842_A7Cfe.seq
>10842_10842_A7Cf_1_CMV_en_R_E11_14_ABI24.ab1     1 GTCGTCTATG ATTACTATTA TAACTAGCTA GTCAATAATC AATGTCGACG ATGATATCAG    61 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA   121 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT   181 GCAATAAACA AGTTAATTCG ATTCAGCTTC AGAAGATGGC GGAGGGCCTC CAACACAGTA   241 ATTTTCCTCC CGACTCTTAA AATAGAAAAT GTCAAGTCAG TTAAGCAGGA AGTGACTAAC   301 TGACCGCAGC TGGCCGTGCG ACATCCTCTT TTAATTAGTT GCTAGGCAAC TGCCCTCCAG   361 AGGGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTCA   421 GTGTGGTTTT GCAAGAGGAA GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC   481 AATCATTACT ATGACAACAG CTGTTTTTTT TAGTATTAAG CAGAGGCCGG GGGCCCCTGG   541 CCTCCGCTTA CTCTGGAGAA AAAGAAGAGA GGCTTCCAGA GGCAACTTGT CAAAACAGGA   601 CTGGCGCCTT GGAGGCGCTG TGGGGCCACC CAAATTGATA TAATTAAGCC CCAACCGCCT   661 CTTCCCGCCT CATTTCAGCC TCACCACTAT GTGAATATAG CTGAATACAC AGTTTATTGA   721 ATAAACATTA ATTTCCAGGA AATACAGTCT TTGTTTTTCC AAAGCGGTCA TTGAAAAAGG   781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTTG CGGCATTTTG   841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT   901 GGGTGCACGA GTGGGTTACA TCGAAACTGG GATCTCAACA GCGGTAAGAT CCCTTGAGAG   961 TTTTCGCCCC GAAGACGTTT TCCAATGAAT GAGCACTTTT TAAGTCTGCT ATGTGGGCGC  1021 GGTATTATCC CGTAATGACG CCGGCAGAGC CAACTCGTCG CCGGCATAAC CTATCTCAGA 1081 ATGGACCTTG GTGGAGTACT CTCACCAGGT TCCAACG //
Primer: CMV_en_R Sequence file: RDB10842_A7Cfe.seq
>10842_10842_A7Cf_1_CMV_en_R_E11_14_ABI24.ab1     1 GTCGTCTATG ATTACTATTA TAACTAGCTA GTCAATAATC AATGTCGACG ATGATATCAG    61 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA   121 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT   181 GCAATAAACA AGTTAATTCG ATTCAGCTTC AGAAGATGGC GGAGGGCCTC CAACACAGTA   241 ATTTTCCTCC CGACTCTTAA AATAGAAAAT GTCAAGTCAG TTAAGCAGGA AGTGACTAAC   301 TGACCGCAGC TGGCCGTGCG ACATCCTCTT TTAATTAGTT GCTAGGCAAC TGCCCTCCAG   361 AGGGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTCA   421 GTGTGGTTTT GCAAGAGGAA GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC   481 AATCATTACT ATGACAACAG CTGTTTTTTT TAGTATTAAG CAGAGGCCGG GGGCCCCTGG   541 CCTCCGCTTA CTCTGGAGAA AAAGAAGAGA GGCTTCCAGA GGCAACTTGT CAAAACAGGA   601 CTGGCGCCTT GGAGGCGCTG TGGGGCCACC CAAATTGATA TAATTAAGCC CCAACCGCCT   661 CTTCCCGCCT CATTTCAGCC TCACCACTAT GTGAATATAG CTGAATACAC AGTTTATTGA   721 ATAAACATTA ATTTCCAGGA AATACAGTCT TTGTTTTTCC AAAGCGGTCA TTGAAAAAGG   781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTTG CGGCATTTTG   841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT   901 GGGTGCACGA GTGGGTTACA TCGAAACTGG GATCTCAACA GCGGTAAGAT CCCTTGAGAG   961 TTTTCGCCCC GAAGACGTTT TCCAATGAAT GAGCACTTTT TAAGTCTGCT ATGTGGGCGC  1021 GGTATTATCC CGTAATGACG CCGGCAGAGC CAACTCGTCG CCGGCATAAC CTATCTCAGA 1081 ATGGACCTTG GTGGAGTACT CTCACCAGGT TCCAACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl