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pBRPyCAG-Klf2(1)-IP

Expression clone of mouse Klf2

Catalog number RDB10842
Resource name pBRPyCAG-Klf2(1)-IP
Alternative name stock no 1856
Clone info. Expression clone of mouse Klf2.
Vector backbone pBRPyCAG-cHA-IP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Klf2 cDNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank mouse Klf2 (NCBI Gene 16598) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB10842 pBRPyCAG-Klf2(1)-IP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB10842z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB10842p.pdf

Original reference

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10842_A7Cfp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB10842_A7Cfa.seq check
>10842_10842_A7Cf_pAxCA_F1_E05_14_ABI24.ab1
    1 GAGTCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG ATCTAGTCCC TGCCATGGCG
  121 CTCAGCGAGC CTATCTTGCC GTCCTTTGCC ACTTTCGCCA GCCCGTGCGA GCGCGGCCTC
  181 CAGGAGCGCT GGCCGCGAAA TGAACCCGAG GCGGGCGGCA CGGATGAGGA CCTAAACAAC
  241 GTGTTGGACT TCATCCTCTC CATGGGATTG GACGGTCTGG GCGCCGAAAA TCCTCCCGAG
  301 CCCCCGCCGC AGCCCCCGCC GCCTGCCTTC TACTACCCGG AGCCGGGTGC TCCGCCGCCC
  361 TACAGCATCC CCGCGGCCAG CCTGGGAACA GAGCTGCTGC GCCCCGACCT GGACCCGCCT
  421 CAGGGGCCGG CTCTGCACGG CCGCTTCCTC CTCGCGCCTC CCGGGGCGGC TAGTGAAGGC
  481 CGAGCCCCCC GAAGGTTGGA CGGGGGGCGG CTACGGCTGG GCTCCGGGGC CTGGGCCCAC
  541 GGACCGCGGC GGGGCTGAAG CTCGAAGGGG CGGCCCCCAG GGAGCCGAAC AGGGGTGCAT
  601 TGCATGCGGG GTTCCCGCCG GGCGGCCCCC GGCGGCCCTG GGAACACGCC GCCGCTTCAA
  661 GCCCCGAACC GGCCCCTTGG GATTCCCGGC AATCCGGGTC CCCGCAAACC CGGTTTCCCG
  721 GCGGCCTTCG GTCCCGGGCC CAGCTTTCGG CGGGTCCGGG CCCGCGGTTG CACTTCGGGG
  781 CTTCCCGCGG CCTTGGGGGC TTTCGGGTCT TTCAAGGAAC GGCGGCAGCG GCGGGGGGGG
  841 CCTTGTGGCT TTTGTTTTCA ACTGTGCCAA GGGGGGTTTC TTAACAGCGC GCCTTTGTTT
  901 CGCTTGAGAA CTTGCTGTGA AGAGGAAAAG CTATACGCGG GGCCGCGTTC TTGTGTTCCG
  961 GAAGAGAGCC GGCCCACCAC AATATTTGTG GCGCATACCA ACTTGAGGGA AGACTATCAC
1021 AATAAGAAAG A
//
Primer: mKlf2-M_F
Sequence file: RDB10842_A7Cfb.seq check
>10842_10842_A7Cf_1_mKlf2-M_F_A05_02_ABI24.ab1
    1 CTCAGTACGC CTCAGGCCGG CTCTGCACGG CCGCTTCCTC CTCGCGCCTC CCGGGCGGCT
   61 AGTGAAGGCC GAGCCCCCCG AGGTGGACGG CGGCGGCTAC GGCTGCGCTC CGGGCCTGGC
  121 CCACGGACCG CGCGGGCTGA AGCTCGAGGG CGCCCCAGGA GCGACAGGTG CATGCATGCG
  181 GGGTCCCGCC GGCCGCCCCC CGCCGCCCCC GGACACGCCG CCGCTCAGCC CCGACGGCCC
  241 CCTGCGCATC CCGGCATCCG GTCCCCGCAA CCCGTTCCCG CCGCCCTTCG GTCCCGGCCC
  301 CAGCTTCGGC GGTCCCGGCC CCGCGTTGCA CTACGGGCCT CCCGCGCCTG GCGCCTTCGG
  361 TCTTTTCGAG GACGCGGCGG CAGCGGCGGC GGCGCTGGGC TTGGCTCCAC CTGCCACGCG
  421 CGGTCTCCTC ACGCCGCCCT CGTCCCCGCT GGAGCTGCTG GAGGCCAAGC CCAAACGCGG
  481 CCGCCGCTCC TGGCCCCGCA AGCGCGCCGC CACACATACT TGCAGCTACA CCAACTGCGG
  541 CAAGACCTAC ACCAAGAGCT CGCACCTAAA GGCGCATCTG CGTACACACA CAGGTGAGAA
  601 GCCTTATCAT TGCAACTGGG AAGGATGCGG CTGGAAGTTC GCGCGCTCTG ACGAGCTTAC
  661 CCGCCACTAC CGAAAGCACA CAGGTCACAG ACCCTTTCAG TGCCACTTGT GCGACCGGGC
  721 CTTCTCCAGG TCCGACCACC TGGCCTTGCA CATGAAGCGA CACATGTAGC CTGTGACCGG
781 TGCGGCCGCT CGATAAGCTT GATATCGAAT TCCGCCCCCC CCCCCCTCTC CC
//
Primer: IRES-F
Sequence file: RDB10842_A7Cfc.seq check
>10842_10842_A7Cf_1_IRES-F_C11_08_ABI24.ab1
    1 TGCATCATTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC
  121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG
  181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG
  241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
  301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
  361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
  421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
  481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
  541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
  601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
  661 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC
  721 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATCTAG AGCTCGCTGA
  781 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT
  841 TCCTTGACCC TGGAAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC
  901 ATCGCATTGT CTGAGTAGTG TCATTCTATT CCTGGGGGGT GGGGGTGGGG CAGACAGCAG
  961 GGGAGGAAAT TGGGGAAAGA ACAAAAAGCA GGCATGCTGG GGATGCCGGT GGGCTTCTTT
 1021 ATGGCTTCTG AGGCGGAAAG GAACTGCAGC CAAGCTGACG TATTCATGTC ATAGCTGATT
1081 CCTGGTTGAA ATGGATATCC GGCCTCACAA AGTTCGCCAC CAAGCGAT
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB10842_A7Cfe.seq check
>10842_10842_A7Cf_1_CMV_en_R_E11_14_ABI24.ab1
    1 GTCGTCTATG ATTACTATTA TAACTAGCTA GTCAATAATC AATGTCGACG ATGATATCAG
   61 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA
  121 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT
  181 GCAATAAACA AGTTAATTCG ATTCAGCTTC AGAAGATGGC GGAGGGCCTC CAACACAGTA
  241 ATTTTCCTCC CGACTCTTAA AATAGAAAAT GTCAAGTCAG TTAAGCAGGA AGTGACTAAC
  301 TGACCGCAGC TGGCCGTGCG ACATCCTCTT TTAATTAGTT GCTAGGCAAC TGCCCTCCAG
  361 AGGGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTCA
  421 GTGTGGTTTT GCAAGAGGAA GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC
  481 AATCATTACT ATGACAACAG CTGTTTTTTT TAGTATTAAG CAGAGGCCGG GGGCCCCTGG
  541 CCTCCGCTTA CTCTGGAGAA AAAGAAGAGA GGCTTCCAGA GGCAACTTGT CAAAACAGGA
  601 CTGGCGCCTT GGAGGCGCTG TGGGGCCACC CAAATTGATA TAATTAAGCC CCAACCGCCT
  661 CTTCCCGCCT CATTTCAGCC TCACCACTAT GTGAATATAG CTGAATACAC AGTTTATTGA
  721 ATAAACATTA ATTTCCAGGA AATACAGTCT TTGTTTTTCC AAAGCGGTCA TTGAAAAAGG
  781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTTG CGGCATTTTG
  841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT
  901 GGGTGCACGA GTGGGTTACA TCGAAACTGG GATCTCAACA GCGGTAAGAT CCCTTGAGAG
  961 TTTTCGCCCC GAAGACGTTT TCCAATGAAT GAGCACTTTT TAAGTCTGCT ATGTGGGCGC
 1021 GGTATTATCC CGTAATGACG CCGGCAGAGC CAACTCGTCG CCGGCATAAC CTATCTCAGA
1081 ATGGACCTTG GTGGAGTACT CTCACCAGGT TCCAACG
//
Primer: CMV_en_R
Sequence file: RDB10842_A7Cfe.seq check
>10842_10842_A7Cf_1_CMV_en_R_E11_14_ABI24.ab1
    1 GTCGTCTATG ATTACTATTA TAACTAGCTA GTCAATAATC AATGTCGACG ATGATATCAG
   61 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA
  121 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT
  181 GCAATAAACA AGTTAATTCG ATTCAGCTTC AGAAGATGGC GGAGGGCCTC CAACACAGTA
  241 ATTTTCCTCC CGACTCTTAA AATAGAAAAT GTCAAGTCAG TTAAGCAGGA AGTGACTAAC
  301 TGACCGCAGC TGGCCGTGCG ACATCCTCTT TTAATTAGTT GCTAGGCAAC TGCCCTCCAG
  361 AGGGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTCA
  421 GTGTGGTTTT GCAAGAGGAA GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC
  481 AATCATTACT ATGACAACAG CTGTTTTTTT TAGTATTAAG CAGAGGCCGG GGGCCCCTGG
  541 CCTCCGCTTA CTCTGGAGAA AAAGAAGAGA GGCTTCCAGA GGCAACTTGT CAAAACAGGA
  601 CTGGCGCCTT GGAGGCGCTG TGGGGCCACC CAAATTGATA TAATTAAGCC CCAACCGCCT
  661 CTTCCCGCCT CATTTCAGCC TCACCACTAT GTGAATATAG CTGAATACAC AGTTTATTGA
  721 ATAAACATTA ATTTCCAGGA AATACAGTCT TTGTTTTTCC AAAGCGGTCA TTGAAAAAGG
  781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTTG CGGCATTTTG
  841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT
  901 GGGTGCACGA GTGGGTTACA TCGAAACTGG GATCTCAACA GCGGTAAGAT CCCTTGAGAG
  961 TTTTCGCCCC GAAGACGTTT TCCAATGAAT GAGCACTTTT TAAGTCTGCT ATGTGGGCGC
 1021 GGTATTATCC CGTAATGACG CCGGCAGAGC CAACTCGTCG CCGGCATAAC CTATCTCAGA
1081 ATGGACCTTG GTGGAGTACT CTCACCAGGT TCCAACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl