RDB10842 - pBRPyCAG-Klf2(1)-IP

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pBRPyCAG-Klf2(1)-IP (#RDB10842)

Expression clone of mouse Klf2

Alternative name	stock no 1856
Clone info.	Expression clone of mouse Klf2.
Vector backbone	pBRPyCAG-cHA-IP (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	Mouse Klf2 cDNA
Depositor\|Developer	Niwa, Hitoshi \|

Sequence	RDB10842z.seq
Remarks, protocol and/or map (pdf)	RDB10842p.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatake, Y., Mol. Cell. Biol. 26 (20): 7772-7782, 2006)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB10842	pBRPyCAG-Klf2(1)-IP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBRPyCAG-Klf2(1)-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10842).

Reference section:

Nakatake, Y., Fukui, N., Iwamatsu, Y., Masui, S., Takahashi, K., Yagi, R., Yagi, K., Miyazaki, J., Matoba, R., Ko, M.S., Niwa, H., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB10842_A7Cfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB10842_A7Cfa.seq
>10842_10842_A7Cf_pAxCA_F1_E05_14_ABI24.ab1 1 GAGTCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT 61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG ATCTAGTCCC TGCCATGGCG 121 CTCAGCGAGC CTATCTTGCC GTCCTTTGCC ACTTTCGCCA GCCCGTGCGA GCGCGGCCTC 181 CAGGAGCGCT GGCCGCGAAA TGAACCCGAG GCGGGCGGCA CGGATGAGGA CCTAAACAAC 241 GTGTTGGACT TCATCCTCTC CATGGGATTG GACGGTCTGG GCGCCGAAAA TCCTCCCGAG 301 CCCCCGCCGC AGCCCCCGCC GCCTGCCTTC TACTACCCGG AGCCGGGTGC TCCGCCGCCC 361 TACAGCATCC CCGCGGCCAG CCTGGGAACA GAGCTGCTGC GCCCCGACCT GGACCCGCCT 421 CAGGGGCCGG CTCTGCACGG CCGCTTCCTC CTCGCGCCTC CCGGGGCGGC TAGTGAAGGC 481 CGAGCCCCCC GAAGGTTGGA CGGGGGGCGG CTACGGCTGG GCTCCGGGGC CTGGGCCCAC 541 GGACCGCGGC GGGGCTGAAG CTCGAAGGGG CGGCCCCCAG GGAGCCGAAC AGGGGTGCAT 601 TGCATGCGGG GTTCCCGCCG GGCGGCCCCC GGCGGCCCTG GGAACACGCC GCCGCTTCAA 661 GCCCCGAACC GGCCCCTTGG GATTCCCGGC AATCCGGGTC CCCGCAAACC CGGTTTCCCG 721 GCGGCCTTCG GTCCCGGGCC CAGCTTTCGG CGGGTCCGGG CCCGCGGTTG CACTTCGGGG 781 CTTCCCGCGG CCTTGGGGGC TTTCGGGTCT TTCAAGGAAC GGCGGCAGCG GCGGGGGGGG 841 CCTTGTGGCT TTTGTTTTCA ACTGTGCCAA GGGGGGTTTC TTAACAGCGC GCCTTTGTTT 901 CGCTTGAGAA CTTGCTGTGA AGAGGAAAAG CTATACGCGG GGCCGCGTTC TTGTGTTCCG 961 GAAGAGAGCC GGCCCACCAC AATATTTGTG GCGCATACCA ACTTGAGGGA AGACTATCAC 1021 AATAAGAAAG A //
Primer: mKlf2-M_F Sequence file: RDB10842_A7Cfb.seq
>10842_10842_A7Cf_1_mKlf2-M_F_A05_02_ABI24.ab1 1 CTCAGTACGC CTCAGGCCGG CTCTGCACGG CCGCTTCCTC CTCGCGCCTC CCGGGCGGCT 61 AGTGAAGGCC GAGCCCCCCG AGGTGGACGG CGGCGGCTAC GGCTGCGCTC CGGGCCTGGC 121 CCACGGACCG CGCGGGCTGA AGCTCGAGGG CGCCCCAGGA GCGACAGGTG CATGCATGCG 181 GGGTCCCGCC GGCCGCCCCC CGCCGCCCCC GGACACGCCG CCGCTCAGCC CCGACGGCCC 241 CCTGCGCATC CCGGCATCCG GTCCCCGCAA CCCGTTCCCG CCGCCCTTCG GTCCCGGCCC 301 CAGCTTCGGC GGTCCCGGCC CCGCGTTGCA CTACGGGCCT CCCGCGCCTG GCGCCTTCGG 361 TCTTTTCGAG GACGCGGCGG CAGCGGCGGC GGCGCTGGGC TTGGCTCCAC CTGCCACGCG 421 CGGTCTCCTC ACGCCGCCCT CGTCCCCGCT GGAGCTGCTG GAGGCCAAGC CCAAACGCGG 481 CCGCCGCTCC TGGCCCCGCA AGCGCGCCGC CACACATACT TGCAGCTACA CCAACTGCGG 541 CAAGACCTAC ACCAAGAGCT CGCACCTAAA GGCGCATCTG CGTACACACA CAGGTGAGAA 601 GCCTTATCAT TGCAACTGGG AAGGATGCGG CTGGAAGTTC GCGCGCTCTG ACGAGCTTAC 661 CCGCCACTAC CGAAAGCACA CAGGTCACAG ACCCTTTCAG TGCCACTTGT GCGACCGGGC 721 CTTCTCCAGG TCCGACCACC TGGCCTTGCA CATGAAGCGA CACATGTAGC CTGTGACCGG 781 TGCGGCCGCT CGATAAGCTT GATATCGAAT TCCGCCCCCC CCCCCCTCTC CC //
Primer: IRES-F Sequence file: RDB10842_A7Cfc.seq
>10842_10842_A7Cf_1_IRES-F_C11_08_ABI24.ab1 1 TGCATCATTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA 61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC 121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG 181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG 241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG 301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG 361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC 421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG 481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC 541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC 601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG 661 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC 721 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATGATCTAG AGCTCGCTGA 781 TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 841 TCCTTGACCC TGGAAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 901 ATCGCATTGT CTGAGTAGTG TCATTCTATT CCTGGGGGGT GGGGGTGGGG CAGACAGCAG 961 GGGAGGAAAT TGGGGAAAGA ACAAAAAGCA GGCATGCTGG GGATGCCGGT GGGCTTCTTT 1021 ATGGCTTCTG AGGCGGAAAG GAACTGCAGC CAAGCTGACG TATTCATGTC ATAGCTGATT 1081 CCTGGTTGAA ATGGATATCC GGCCTCACAA AGTTCGCCAC CAAGCGAT //
Primer: SV40pA3'Element Reverse Sequence file: RDB10842_A7Cfe.seq
>10842_10842_A7Cf_1_CMV_en_R_E11_14_ABI24.ab1 1 GTCGTCTATG ATTACTATTA TAACTAGCTA GTCAATAATC AATGTCGACG ATGATATCAG 61 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 121 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT 181 GCAATAAACA AGTTAATTCG ATTCAGCTTC AGAAGATGGC GGAGGGCCTC CAACACAGTA 241 ATTTTCCTCC CGACTCTTAA AATAGAAAAT GTCAAGTCAG TTAAGCAGGA AGTGACTAAC 301 TGACCGCAGC TGGCCGTGCG ACATCCTCTT TTAATTAGTT GCTAGGCAAC TGCCCTCCAG 361 AGGGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTCA 421 GTGTGGTTTT GCAAGAGGAA GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC 481 AATCATTACT ATGACAACAG CTGTTTTTTT TAGTATTAAG CAGAGGCCGG GGGCCCCTGG 541 CCTCCGCTTA CTCTGGAGAA AAAGAAGAGA GGCTTCCAGA GGCAACTTGT CAAAACAGGA 601 CTGGCGCCTT GGAGGCGCTG TGGGGCCACC CAAATTGATA TAATTAAGCC CCAACCGCCT 661 CTTCCCGCCT CATTTCAGCC TCACCACTAT GTGAATATAG CTGAATACAC AGTTTATTGA 721 ATAAACATTA ATTTCCAGGA AATACAGTCT TTGTTTTTCC AAAGCGGTCA TTGAAAAAGG 781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTTG CGGCATTTTG 841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 901 GGGTGCACGA GTGGGTTACA TCGAAACTGG GATCTCAACA GCGGTAAGAT CCCTTGAGAG 961 TTTTCGCCCC GAAGACGTTT TCCAATGAAT GAGCACTTTT TAAGTCTGCT ATGTGGGCGC 1021 GGTATTATCC CGTAATGACG CCGGCAGAGC CAACTCGTCG CCGGCATAAC CTATCTCAGA 1081 ATGGACCTTG GTGGAGTACT CTCACCAGGT TCCAACG //
Primer: CMV_en_R Sequence file: RDB10842_A7Cfe.seq
>10842_10842_A7Cf_1_CMV_en_R_E11_14_ABI24.ab1 1 GTCGTCTATG ATTACTATTA TAACTAGCTA GTCAATAATC AATGTCGACG ATGATATCAG 61 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 121 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT 181 GCAATAAACA AGTTAATTCG ATTCAGCTTC AGAAGATGGC GGAGGGCCTC CAACACAGTA 241 ATTTTCCTCC CGACTCTTAA AATAGAAAAT GTCAAGTCAG TTAAGCAGGA AGTGACTAAC 301 TGACCGCAGC TGGCCGTGCG ACATCCTCTT TTAATTAGTT GCTAGGCAAC TGCCCTCCAG 361 AGGGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTCA 421 GTGTGGTTTT GCAAGAGGAA GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC 481 AATCATTACT ATGACAACAG CTGTTTTTTT TAGTATTAAG CAGAGGCCGG GGGCCCCTGG 541 CCTCCGCTTA CTCTGGAGAA AAAGAAGAGA GGCTTCCAGA GGCAACTTGT CAAAACAGGA 601 CTGGCGCCTT GGAGGCGCTG TGGGGCCACC CAAATTGATA TAATTAAGCC CCAACCGCCT 661 CTTCCCGCCT CATTTCAGCC TCACCACTAT GTGAATATAG CTGAATACAC AGTTTATTGA 721 ATAAACATTA ATTTCCAGGA AATACAGTCT TTGTTTTTCC AAAGCGGTCA TTGAAAAAGG 781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTTG CGGCATTTTG 841 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 901 GGGTGCACGA GTGGGTTACA TCGAAACTGG GATCTCAACA GCGGTAAGAT CCCTTGAGAG 961 TTTTCGCCCC GAAGACGTTT TCCAATGAAT GAGCACTTTT TAAGTCTGCT ATGTGGGCGC 1021 GGTATTATCC CGTAATGACG CCGGCAGAGC CAACTCGTCG CCGGCATAAC CTATCTCAGA 1081 ATGGACCTTG GTGGAGTACT CTCACCAGGT TCCAACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384. [link to RRC of NBRP]