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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBRblue-MC1DTApA

Expression clone of diphtheria toxin (DT-A)

Catalog number RDB10550
Resource name pBRblue-MC1DTApA
Alternative name stock no 1555, pMC1DTApA
Clone info. Expression clone of diphtheria toxin (DT-A) under the control of MC1, polyoma enhancer and tk promoter.
Vector backbone pBluescript II KS (-) (Plasmid)
Gene/insert name Corynebacterium diphtheriae Diphtheria toxin A genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank Corynebacterium diphtheriae tox (KEGG orthology K00776) |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested ().
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RDB10550 pBRblue-MC1DTApA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB10550z.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10550_A3Jap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Region: MC1 pro, DTA 5'
Sequence file: RDB10550_A3Jaa.seq check
>10550_10550_A9KcF_3_M13_-40_C08_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNNGNCTNN CTATAGGGCG AANTGGAGCT CCACCGCGGT
   61 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATAT CAAGCTTATC
  121 GATACCGTCG AGCAGTGTGG TTTTCAAGAG GAAGCAAAAA GCCTCTCCAC CCAGGCCTGG
  181 AATGTTTCCA CCCAATGTCG AGCAGTGTGG TTTTGCAAGA GGAAGCAAAA AGCCTCTCCA
  241 CCCAGGCCTG GAATGTTTCC ACCCAATGTC GAGCAAACCC CGCCCAGCGT CTTGTCATTG
  301 GCGAATTCGA ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT
  361 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GGTCCTCGCC ATGGATCCTG
  421 ATGATGTTGT TGATTCTTCT AAATCTTTTG TGATGGAAAA CTTTTCTTCG TACCACGGGA
  481 CTAAACCTGG TTATGTAGAT TCCATTCAAA AAGGTATACA AAAGCCAAAA TCTGGTACAC
  541 AAGGAAATTA TGACGATGAT TGGAAAGGGT TTTATAGTAC CGACAATAAA TACGACGCTG
  601 CGGGATACTC TGTAGATAAT GAAAACCCGC TCTCTGGAAA AGCTGGAGGC GTGGTCAAAG
  661 TGACGTATCC AGGACTGACG AANGTTCTCG CACTAAAAGT GGATAATGCC GAAACTATTA
  721 AGAAAGAGTT AGGTTTAAGT CTCACTGAAC CGTTGATGGA GCAAGTCGGA ACGGAAGAGT
  781 TTATCAAAAG GNTCGGTGAT GGTGCTTCGC GTGTAGTGCT CAGCCTTCCC TTCGCTGANG
  841 GGAGTTCTNG CGTTGAATAT ATTANTNNNT GGGAACANGC GAAAGCGTTA ANCGTAGAAC
  901 NTGAGANTAA TTTTGAAATC CGNGGAAAAN GNNNCCNAGA TGCNATGTAT GAGTATATNG
  961 NNTNNANCCN GNGCNNNAAA TCNNGNNNNN NATNTNNTNN TGNANGGNAN NTTNNTNCTG
1021 NGGNGNGANN TNNNTNGNNN NANNNNNNNN NN
//
Primer: LTAlarge_upR
Region: DTA
Sequence file: RDB10550_A3Jab.seq check
>10550_10550_A9KcF_3_LTAlarge_upR_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCA AACNATTAGN ANCAGTAGTT TAACACATTA TACACTTAAA
   61 AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA TTATGTCACA
  121 CCACAGAAGT AAGGTTCCTT CACAAAGAGA TCGCCTGACA CGATTTCCTG CACAGGCTTG
  181 AGCCATATAC TCATACATCG CATCTTGGCC ACGTTTTCCA CGGATTTCAA AATTAATCTC
  241 AAGTTCTACG CTTAACGCTT TCGCCTGTTC CCAGTTATTA ATATATTCAA CGCTAGAACT
  301 CCCCTCAGCG AAGGGAAGGC TGAGCACTAC ACGCGAAGCA CCATCACCGA ACCTTTTGAT
  361 AAACTCTTCC GTTCCGACTT GCTCCATCAA CGGTTCAGTG AGACTTAAAC CTAACTCTTT
  421 CTTAATAGTT TCGGCATTAT CCACTTTTAG TGCGAGAACC TTCGTCAGTC CTGGATACGT
  481 CACTTTGACC ACGCCTCCAG CTTTTCCAGA GAGCGGGTTT TCATTATCTA CAGAGTATCC
  541 CGCAGCGTCG TATTTATTGT CGGTACTATA AAACCCTTTC CAATCATCGT CATAATTTCC
  601 TTGTGTACCA GATTTTGGCT TTTGTATACC TTTTTGAATG GAATCTACAT AACCAGGTTT
  661 AGTCCCGTGG TACGAAGAAA AGTTTTCCAT CACAAAAGAT TTAGAAGAAT CAACAACATC
  721 ATCAGGATCC ATGGCGAGGA CCTGCAGGGT CGCTCGGTGT TCGANGCCAC ACGCGTCACC
  781 TTAATATGCG AANTGGANCT NNNANCGCGC CGCCCCGACN GCATCTGCGT GTTCGAATTC
  841 GCCAATGACA ANANGCTGGG CGGGGTTNGC TCGACATNNG GGNNGAAACA TTCCAGGCCN
  901 GGNNNGANNN GNTTTTTNGC TNCCNNTTGN NAAANCNCAC NGNNNGNNNN NNGNNNGNNN
961 NNTTCCNNNN CNNNNNNNNN N
//
Primer: reverse-2
Region: PGK p(A)
Sequence file: RDB10550_A3Jac.seq check
>10550_10550_A9KcF_3_reverse2_D08_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGCNN TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
   61 GTACCGGGCC CCCCCTCGAG GTCGGCCGCA TCTAGCTAGA GTCGATCGAC CAGCTTCTGA
  121 TGGAATTAGA ACTTGGCAAA ACAATACTGA GAATGAAGTG TATGTGGAAC AGAGGCTGCT
  181 GATCTCGTTC TTCAGGCTAT GAAACTGACA CATTTGGAAA CCACAGTACT TAGAACCACA
  241 AAGTGGGAAT CAAGAGAAAA ACAATGATCC CACGAGAGAT CTATAGATCT ATAGATCATG
  301 AGTGGGAGGA ATGAGCTGGC CCTTAATTTG GTTTTGCTTG TTTAAATTAT GATATCCAAC
  361 TATGAAACAT TATCATAAAG CAATAGTAAA GAGCCTTCAG TAAAGAGCAG GCATTTATCT
  421 AATCCCACCC CACCCCCACC CCCGTAGCTC CAATCCTTCC ATTCAAAATG TAGGTACTCT
  481 GTTCTCACCC TTCTTAACAA AGTATGACAG GAAAAACTTC CATTTTAGTG GACATCTTTA
  541 TTGTTTAATA GATCATCAAT TTCTGCATCC CTCGACTCTA GTGGATCTGC ATTCCACCAC
  601 TGCTCCCATT CATCAGTTCC ATAGGTTGGA ATCTAAAATA CACAAACAAT TAGAATCAGT
  661 AGTTTAACAC ATTATACACT TAAAAATTTT ATATTTACCT TAGAGCTTTA AATCTCTGTA
  721 GGTAGTTTGT CCAATTATGT CACACCACAG AAGTAANGTT CCTTCACAAA GAGATCGCCT
  781 GACACGATTT CCTGCACAGG CTTGAGCCAT ATACTCATAC ATCGCATCTN GGCCACGTTT
  841 TCCACGGATT TCAAAATTAA TCTCANGTTC TACGCTTAAC GCTTNCGNCN GTNNCNAGTT
  901 ATTAATATAN TCAACGCTAG AACTCCCNTC ANCGNANNGN NANGGNTGAN CNCTACNCGC
961 NNANCANCNN CACCGNANCN TTTTGANAAA CTCTTNCNGT NNCNANNTGN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

GNP_filter3_RDBDEP_html_200316.pl