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pPyPGK-cHA-IP

Expression vector with mouse PGK promoter, HA-tag, IRESpuro.

Catalog number RDB10479
Resource name pPyPGK-cHA-IP
Alternative name stock no 1479
Clone info. Expression vector with mouse PGK promoter, HA-tag, IRESpuro, bovine groth hormone poly(A) and SV40 ori and polyoma ori.
Vector backbone pPGK-cHA-IP (Plasmid)
Depositor|Developer Niwa, Hitoshi |
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            Reference sequence
              

            Distribution information

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            RDB10479 pPyPGK-cHA-IP DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB10479p.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

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            Featured content Empty Backbone (English text)
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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB10479_A8L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pGKpro_F
            Sequence file: RDB10479_A8L7a.seq check
            >10479_10479_A8L7_B1_pGKpro_F_E07_13_ABI24.ab1
                1 CCCGTATCTC TCTTCTCATC TCCGGGCCTT TCTCGAGATC TCACGCGTGA CTAGTGCCAT
               61 GGCCTACCCA TACGACGTGC CAGACTACGC CTAGCGGCCG CTCGATAAGC TTGATATCGA
              121 ATTCCGCCCC CCCCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG
              181 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA
              241 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC
              301 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG
              361 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG
              421 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC
              481 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA
              541 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC
              601 TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC
              661 CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC
              721 ATGACCGAGT ACAAAGCCCA CGGTGCGCCT CGGCCACCCG CGACGACGTC CCCAAGGGCC
              781 GTACGCACCC TCGCCGCCCG CGTTTCGCCG ACTACCCCGG CCACGCGCCA CACCGGTCGA
              841 TCCGGGACCG CCACATCGGA GCGGGTCACC GAAGCTGCCA AGGAACTCTT TCCTCACGCG
              901 CCGTCGGGGC TCGACATCGG CAAGGGTGGT GGGTCGCCGG AACGACGGCC GCCCGCGGTT
              961 GGGCGGGTCT GGGAACAACG CCCGGAGAAG CGTTCAAGCC GGGGGGCGGG TGTTTCGCCG
             1021 GAGGATTCGG CCCCGGCGCC ATGGGACCGG AAGTTGAAGG CAGGGATTCC CGGCTGGACC
            1081 GCGGCAGTCA ACCAGGAAAT GGGAGGGCCC TTCCCTGGCG TGC
            //
            Primer: BGH_rev
            Sequence file: RDB10479_A8L7b.seq check
            >10479_10479_A8L7_B1_BGH_rev_B01_02_ABI08.ab1
                1 TAACTTAAAA AATCGATGCA TGGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG
               61 TGGGGCGGGC GTCAGGCACC GGGCTTGCGG GTCATGCACC AGGTGCGCGG TCCTTCGGGC
              121 ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT AGAAGGGGAG GTTGCGGGGC
              181 GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG CCGCCTCCAC TCCGGGGAGC
              241 ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG AGACGCCGAC GGTGGCCAGG
              301 AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC CTTCCATCTG TTGCTGCGCG
              361 GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA TCTCGGCGAA CACCGCCCCC
              421 GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG CGCCGTCGTC CGCGACCCAC
              481 ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT CTTGCAGCTC GGTGACCCGC
              541 TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG GGTAGTCGGC GAACGCGGCG
              601 GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG CGAGGCGCAC CGTGGGCTTG
              661 TACTCGGTCA TGGTTGTGGC CATATTATCA TCGTGTTTTT CAAAGGAAAA CCACGTCCCC
              721 GTGGTTCGGG GGGCCTAGAC GTTTTTTTAA CCTCGACTAA ACACATGTAA AGCATGTGCA
              781 CCGAGGCCCC AGATCAGATC CCATACAATG GGGTACCTTC TGGGCATCCT TCAGCCCCTT
              841 GTTGAATACG CTTGAGGAGA GCCATTTGAC TCTTTCCACA ACTATCCAAC TCACAACGTG
              901 GCACTGGGGG TTGTGCCGCC CTTTGCAGTG TATCTTTATA CACGTGGGCT TTTGGCCGCA
              961 GAGGCACCCT GTCGCCAGGG TGGGGGGGTT TCCGCTGCCT GCAAAAGGGT CGCTAACAGA
             1021 CGTTTGTTTG TCTTCCAAGA AGCCTTCCCA GAAGGAAACT GCTTCCCTTT CACGGACCAT
             1081 TTCAAACAGA CCCTTTGCAT TCCTTTGTGA GGAAGGGGGA AAGAACCCCT TAGAATGCCT
            1141 CGGTCAGATA AGAACAAGGG CCAAGGTTTT TTCCGGGGGG ACTCTC
            //
            Primer: Amp_R
            Sequence file: RDB10479_A8L7c.seq check
            >10479_10479_A8L7_B1_Amp_R_D01_04_ABI08.ab1
                1 CTTTTTTTTT TCATGGACCG CTTTGGAAAA CAAAGACTGT ATTTCCTGGA AATTAATGTT
               61 TATTCAATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG
              121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC
              181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG
              241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG
              301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA
              361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC
              421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG
              481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA
              541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT
              601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
              661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
              721 GATCTGATAT CATCGTCGAC CCTGCAGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA
              781 GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA
              841 TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT
              901 CCTCCCCTAG TCAGGAAGTT CCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GACGTGACAA
              961 ATGGAAGTAG CACGTCTCTC TAGACTCGTG CAGATGGACA GCACCCGCTG AGCAATGGAA
             1021 GCGGTAGGCC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTTCGCT TTCTGGGCTC
             1081 AGAGGCTGGA GGGAGTGGAT CAGAGCCGGA CTCAAGGGCC GGCCCTCAGG AGCCGGGAGC
            1141 AG
            //
            Primer: IRES-R2
            Sequence file: RDB10479_A8L7d.seq check
            >10479_10479_A8L7_B1_IRES-R2_100_G07_19_ABI24.ab1
                1 ATTTCAGGCG GGCTTCGGCC AGTAACGTTA GGGGGAGGGG GAGGGAGAGG GGGGGGGGGC
               61 GGAATTCGAT ATCAAGCTTA TCGAGCGGCC GCTAGGCGTA GTCTGGCACG TCGTATGGGT
              121 AGGCCATGGC ACTAGTCACG CGTGAGATCT CGAGAAAGGC CCGGAGATGA GGAAGAGGAG
              181 AACAGCGCGG CAGACGTGCG CTTTTGAAGC GTGCAGAATG CCGGGCCTCC GGAGGACCTT
              241 CGGGCGCCCG CCCCGCCCCT GAGCCCGCCC CTGAGCCCGC CCCCGGACCC ACCCCTTCCC
              301 AGCCTCTGAG CCCAGAAAGC GAAGGAGCAA AGCTGCTATT GGCCGCTGCC CCAAAGGCCT
              361 ACCCGCTTCC ATTGCTCAGC GGTGCTGTCC ATCTGCACGA GTCTAGAGAG ACGTGCTACT
              421 TCCATTTGTC ACGTCCTGCA CGACGCGAGC TGCGGGGCGG GGGGGAACTT CCTGACTAGG
              481 GGAGGAGTAG AAGGTGGCGC GAAGGGGCCA CCAAAGAACG GAGCCGGTTG GCGCCTACCG
              541 GTGGATGTGG AATGTGTGCG AGGCCAGAGG CCACTTGTGT AGCGCCAAGT GCCCAGCGGG
              601 GCTGCTAAAG CGCATGCTCC AGACTGCCTT GGGAAAAGCT GCAGGGTCGA CGATGATATC
              661 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
              721 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
              781 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCCAACACA
              841 GGTAATTTTC CTCCCGACTC TTAAAATAGA AAAATGTCAA GTCAGTTAAG CAGGAAGTGG
              901 ACTTAACTGA CCGGCAGCTG GCCGGTGCGA CATCCTCTTT TTAATTAAGT TTGCTAGGGC
              961 AAACTGCCCT TCCAGAGGGG CAGTGTTGGT TTTTGCAAAG AGGGAAGCAA AAAAGCCTTC
             1021 TCCACCCCAA GCCCTGGGAA ATGTCCAGTG GTGGATTTTG CAAGGAAGGA AGCCAAAAAA
            1081 GCCTCTTCCA CTCAGGCCTT GAATGGTTTT CCACGCCCAG AGGTCCGGCG AGT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl