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pPyPGK-cHA-IP

Expression vector with mouse PGK promoter, HA-tag, IRESpuro.

Catalog number RDB10479
Resource name pPyPGK-cHA-IP
Alternative name stock no 1479
Clone info. Expression vector with mouse PGK promoter, HA-tag, IRESpuro, bovine groth hormone poly(A) and SV40 ori and polyoma ori.
Vector backbone pPGK-cHA-IP (Plasmid)
Depositor Niwa, Hitoshi |

Distribution information

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RDB10479 pPyPGK-cHA-IP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB10479p.pdf from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB10479_A8L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGKpro_F RDB10479_A8L7a.seq
>10479_10479_A8L7_B1_pGKpro_F_E07_13_ABI24.ab1
    1 CCCGTATCTC TCTTCTCATC TCCGGGCCTT TCTCGAGATC TCACGCGTGA CTAGTGCCAT
   61 GGCCTACCCA TACGACGTGC CAGACTACGC CTAGCGGCCG CTCGATAAGC TTGATATCGA
  121 ATTCCGCCCC CCCCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG
  181 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA
  241 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC
  301 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG
  361 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG
  421 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC
  481 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA
  541 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC
  601 TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC
  661 CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC
  721 ATGACCGAGT ACAAAGCCCA CGGTGCGCCT CGGCCACCCG CGACGACGTC CCCAAGGGCC
  781 GTACGCACCC TCGCCGCCCG CGTTTCGCCG ACTACCCCGG CCACGCGCCA CACCGGTCGA
  841 TCCGGGACCG CCACATCGGA GCGGGTCACC GAAGCTGCCA AGGAACTCTT TCCTCACGCG
  901 CCGTCGGGGC TCGACATCGG CAAGGGTGGT GGGTCGCCGG AACGACGGCC GCCCGCGGTT
  961 GGGCGGGTCT GGGAACAACG CCCGGAGAAG CGTTCAAGCC GGGGGGCGGG TGTTTCGCCG
 1021 GAGGATTCGG CCCCGGCGCC ATGGGACCGG AAGTTGAAGG CAGGGATTCC CGGCTGGACC
1081 GCGGCAGTCA ACCAGGAAAT GGGAGGGCCC TTCCCTGGCG TGC
//
Primer: BGH_rev RDB10479_A8L7b.seq
>10479_10479_A8L7_B1_BGH_rev_B01_02_ABI08.ab1
    1 TAACTTAAAA AATCGATGCA TGGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG
   61 TGGGGCGGGC GTCAGGCACC GGGCTTGCGG GTCATGCACC AGGTGCGCGG TCCTTCGGGC
  121 ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT AGAAGGGGAG GTTGCGGGGC
  181 GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG CCGCCTCCAC TCCGGGGAGC
  241 ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG AGACGCCGAC GGTGGCCAGG
  301 AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC CTTCCATCTG TTGCTGCGCG
  361 GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA TCTCGGCGAA CACCGCCCCC
  421 GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG CGCCGTCGTC CGCGACCCAC
  481 ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT CTTGCAGCTC GGTGACCCGC
  541 TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG GGTAGTCGGC GAACGCGGCG
  601 GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG CGAGGCGCAC CGTGGGCTTG
  661 TACTCGGTCA TGGTTGTGGC CATATTATCA TCGTGTTTTT CAAAGGAAAA CCACGTCCCC
  721 GTGGTTCGGG GGGCCTAGAC GTTTTTTTAA CCTCGACTAA ACACATGTAA AGCATGTGCA
  781 CCGAGGCCCC AGATCAGATC CCATACAATG GGGTACCTTC TGGGCATCCT TCAGCCCCTT
  841 GTTGAATACG CTTGAGGAGA GCCATTTGAC TCTTTCCACA ACTATCCAAC TCACAACGTG
  901 GCACTGGGGG TTGTGCCGCC CTTTGCAGTG TATCTTTATA CACGTGGGCT TTTGGCCGCA
  961 GAGGCACCCT GTCGCCAGGG TGGGGGGGTT TCCGCTGCCT GCAAAAGGGT CGCTAACAGA
 1021 CGTTTGTTTG TCTTCCAAGA AGCCTTCCCA GAAGGAAACT GCTTCCCTTT CACGGACCAT
 1081 TTCAAACAGA CCCTTTGCAT TCCTTTGTGA GGAAGGGGGA AAGAACCCCT TAGAATGCCT
1141 CGGTCAGATA AGAACAAGGG CCAAGGTTTT TTCCGGGGGG ACTCTC
//
Primer: Amp_R RDB10479_A8L7c.seq
>10479_10479_A8L7_B1_Amp_R_D01_04_ABI08.ab1
    1 CTTTTTTTTT TCATGGACCG CTTTGGAAAA CAAAGACTGT ATTTCCTGGA AATTAATGTT
   61 TATTCAATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG
  121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC
  181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG
  241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG
  301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA
  361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC
  421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG
  481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA
  541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT
  601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
  661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
  721 GATCTGATAT CATCGTCGAC CCTGCAGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA
  781 GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA
  841 TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT
  901 CCTCCCCTAG TCAGGAAGTT CCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GACGTGACAA
  961 ATGGAAGTAG CACGTCTCTC TAGACTCGTG CAGATGGACA GCACCCGCTG AGCAATGGAA
 1021 GCGGTAGGCC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTTCGCT TTCTGGGCTC
 1081 AGAGGCTGGA GGGAGTGGAT CAGAGCCGGA CTCAAGGGCC GGCCCTCAGG AGCCGGGAGC
1141 AG
//
Primer: IRES-R2 RDB10479_A8L7d.seq
>10479_10479_A8L7_B1_IRES-R2_100_G07_19_ABI24.ab1
    1 ATTTCAGGCG GGCTTCGGCC AGTAACGTTA GGGGGAGGGG GAGGGAGAGG GGGGGGGGGC
   61 GGAATTCGAT ATCAAGCTTA TCGAGCGGCC GCTAGGCGTA GTCTGGCACG TCGTATGGGT
  121 AGGCCATGGC ACTAGTCACG CGTGAGATCT CGAGAAAGGC CCGGAGATGA GGAAGAGGAG
  181 AACAGCGCGG CAGACGTGCG CTTTTGAAGC GTGCAGAATG CCGGGCCTCC GGAGGACCTT
  241 CGGGCGCCCG CCCCGCCCCT GAGCCCGCCC CTGAGCCCGC CCCCGGACCC ACCCCTTCCC
  301 AGCCTCTGAG CCCAGAAAGC GAAGGAGCAA AGCTGCTATT GGCCGCTGCC CCAAAGGCCT
  361 ACCCGCTTCC ATTGCTCAGC GGTGCTGTCC ATCTGCACGA GTCTAGAGAG ACGTGCTACT
  421 TCCATTTGTC ACGTCCTGCA CGACGCGAGC TGCGGGGCGG GGGGGAACTT CCTGACTAGG
  481 GGAGGAGTAG AAGGTGGCGC GAAGGGGCCA CCAAAGAACG GAGCCGGTTG GCGCCTACCG
  541 GTGGATGTGG AATGTGTGCG AGGCCAGAGG CCACTTGTGT AGCGCCAAGT GCCCAGCGGG
  601 GCTGCTAAAG CGCATGCTCC AGACTGCCTT GGGAAAAGCT GCAGGGTCGA CGATGATATC
  661 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
  721 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
  781 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCCAACACA
  841 GGTAATTTTC CTCCCGACTC TTAAAATAGA AAAATGTCAA GTCAGTTAAG CAGGAAGTGG
  901 ACTTAACTGA CCGGCAGCTG GCCGGTGCGA CATCCTCTTT TTAATTAAGT TTGCTAGGGC
  961 AAACTGCCCT TCCAGAGGGG CAGTGTTGGT TTTTGCAAAG AGGGAAGCAA AAAAGCCTTC
 1021 TCCACCCCAA GCCCTGGGAA ATGTCCAGTG GTGGATTTTG CAAGGAAGGA AGCCAAAAAA
1081 GCCTCTTCCA CTCAGGCCTT GAATGGTTTT CCACGCCCAG AGGTCCGGCG AGT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.20

GNP_filter3_RDBDEP_html_181012.pl