Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyPGK-cHA-IP (#RDB10479)

Expression vector with mouse PGK promoter, HA-tag, IRESpuro.

Alternative name	stock no 1479
Clone info.	Expression vector with mouse PGK promoter, HA-tag, IRESpuro, bovine groth hormone poly(A) and SV40 ori and polyoma ori.
Vector backbone	pPGK-cHA-IP (Plasmid)
Depositor|Developer	Niwa, Hitoshi |

 

Remarks, protocol and/or map (pdf)	RDB10479p.pdf

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB10479	pPyPGK-cHA-IP	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pPyPGK-cHA-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10479).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB10479_A8L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGKpro_F () Sequence file: RDB10479_A8L7a.seq
>10479_10479_A8L7_B1_pGKpro_F_E07_13_ABI24.ab1     1 CCCGTATCTC TCTTCTCATC TCCGGGCCTT TCTCGAGATC TCACGCGTGA CTAGTGCCAT    61 GGCCTACCCA TACGACGTGC CAGACTACGC CTAGCGGCCG CTCGATAAGC TTGATATCGA   121 ATTCCGCCCC CCCCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG   181 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA   241 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC   301 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG   361 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG   421 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC   481 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA   541 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC   601 TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC   661 CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC   721 ATGACCGAGT ACAAAGCCCA CGGTGCGCCT CGGCCACCCG CGACGACGTC CCCAAGGGCC   781 GTACGCACCC TCGCCGCCCG CGTTTCGCCG ACTACCCCGG CCACGCGCCA CACCGGTCGA   841 TCCGGGACCG CCACATCGGA GCGGGTCACC GAAGCTGCCA AGGAACTCTT TCCTCACGCG   901 CCGTCGGGGC TCGACATCGG CAAGGGTGGT GGGTCGCCGG AACGACGGCC GCCCGCGGTT   961 GGGCGGGTCT GGGAACAACG CCCGGAGAAG CGTTCAAGCC GGGGGGCGGG TGTTTCGCCG  1021 GAGGATTCGG CCCCGGCGCC ATGGGACCGG AAGTTGAAGG CAGGGATTCC CGGCTGGACC 1081 GCGGCAGTCA ACCAGGAAAT GGGAGGGCCC TTCCCTGGCG TGC //
Primer: BGH_rev () Sequence file: RDB10479_A8L7b.seq
>10479_10479_A8L7_B1_BGH_rev_B01_02_ABI08.ab1     1 TAACTTAAAA AATCGATGCA TGGGGGTCGT GCGCTCCTTT CGGTCGGGCG CTGCGGGTCG    61 TGGGGCGGGC GTCAGGCACC GGGCTTGCGG GTCATGCACC AGGTGCGCGG TCCTTCGGGC   121 ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT AGAAGGGGAG GTTGCGGGGC   181 GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG CCGCCTCCAC TCCGGGGAGC   241 ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG AGACGCCGAC GGTGGCCAGG   301 AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC CTTCCATCTG TTGCTGCGCG   361 GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA TCTCGGCGAA CACCGCCCCC   421 GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG CGCCGTCGTC CGCGACCCAC   481 ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT CTTGCAGCTC GGTGACCCGC   541 TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG GGTAGTCGGC GAACGCGGCG   601 GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG CGAGGCGCAC CGTGGGCTTG   661 TACTCGGTCA TGGTTGTGGC CATATTATCA TCGTGTTTTT CAAAGGAAAA CCACGTCCCC   721 GTGGTTCGGG GGGCCTAGAC GTTTTTTTAA CCTCGACTAA ACACATGTAA AGCATGTGCA   781 CCGAGGCCCC AGATCAGATC CCATACAATG GGGTACCTTC TGGGCATCCT TCAGCCCCTT   841 GTTGAATACG CTTGAGGAGA GCCATTTGAC TCTTTCCACA ACTATCCAAC TCACAACGTG   901 GCACTGGGGG TTGTGCCGCC CTTTGCAGTG TATCTTTATA CACGTGGGCT TTTGGCCGCA   961 GAGGCACCCT GTCGCCAGGG TGGGGGGGTT TCCGCTGCCT GCAAAAGGGT CGCTAACAGA  1021 CGTTTGTTTG TCTTCCAAGA AGCCTTCCCA GAAGGAAACT GCTTCCCTTT CACGGACCAT  1081 TTCAAACAGA CCCTTTGCAT TCCTTTGTGA GGAAGGGGGA AAGAACCCCT TAGAATGCCT 1141 CGGTCAGATA AGAACAAGGG CCAAGGTTTT TTCCGGGGGG ACTCTC //
Primer: Amp_R () Sequence file: RDB10479_A8L7c.seq
>10479_10479_A8L7_B1_Amp_R_D01_04_ABI08.ab1     1 CTTTTTTTTT TCATGGACCG CTTTGGAAAA CAAAGACTGT ATTTCCTGGA AATTAATGTT    61 TATTCAATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG   121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC   181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG   241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG   301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA   361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC   421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG   481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA   541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT   601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT   661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG   721 GATCTGATAT CATCGTCGAC CCTGCAGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA   781 GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA   841 TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT   901 CCTCCCCTAG TCAGGAAGTT CCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GACGTGACAA   961 ATGGAAGTAG CACGTCTCTC TAGACTCGTG CAGATGGACA GCACCCGCTG AGCAATGGAA  1021 GCGGTAGGCC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTTCGCT TTCTGGGCTC  1081 AGAGGCTGGA GGGAGTGGAT CAGAGCCGGA CTCAAGGGCC GGCCCTCAGG AGCCGGGAGC 1141 AG //
Primer: IRES-R2 () Sequence file: RDB10479_A8L7d.seq
>10479_10479_A8L7_B1_IRES-R2_100_G07_19_ABI24.ab1     1 ATTTCAGGCG GGCTTCGGCC AGTAACGTTA GGGGGAGGGG GAGGGAGAGG GGGGGGGGGC    61 GGAATTCGAT ATCAAGCTTA TCGAGCGGCC GCTAGGCGTA GTCTGGCACG TCGTATGGGT   121 AGGCCATGGC ACTAGTCACG CGTGAGATCT CGAGAAAGGC CCGGAGATGA GGAAGAGGAG   181 AACAGCGCGG CAGACGTGCG CTTTTGAAGC GTGCAGAATG CCGGGCCTCC GGAGGACCTT   241 CGGGCGCCCG CCCCGCCCCT GAGCCCGCCC CTGAGCCCGC CCCCGGACCC ACCCCTTCCC   301 AGCCTCTGAG CCCAGAAAGC GAAGGAGCAA AGCTGCTATT GGCCGCTGCC CCAAAGGCCT   361 ACCCGCTTCC ATTGCTCAGC GGTGCTGTCC ATCTGCACGA GTCTAGAGAG ACGTGCTACT   421 TCCATTTGTC ACGTCCTGCA CGACGCGAGC TGCGGGGCGG GGGGGAACTT CCTGACTAGG   481 GGAGGAGTAG AAGGTGGCGC GAAGGGGCCA CCAAAGAACG GAGCCGGTTG GCGCCTACCG   541 GTGGATGTGG AATGTGTGCG AGGCCAGAGG CCACTTGTGT AGCGCCAAGT GCCCAGCGGG   601 GCTGCTAAAG CGCATGCTCC AGACTGCCTT GGGAAAAGCT GCAGGGTCGA CGATGATATC   661 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG   721 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG   781 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCCAACACA   841 GGTAATTTTC CTCCCGACTC TTAAAATAGA AAAATGTCAA GTCAGTTAAG CAGGAAGTGG   901 ACTTAACTGA CCGGCAGCTG GCCGGTGCGA CATCCTCTTT TTAATTAAGT TTGCTAGGGC   961 AAACTGCCCT TCCAGAGGGG CAGTGTTGGT TTTTGCAAAG AGGGAAGCAA AAAAGCCTTC  1021 TCCACCCCAA GCCCTGGGAA ATGTCCAGTG GTGGATTTTG CAAGGAAGGA AGCCAAAAAA 1081 GCCTCTTCCA CTCAGGCCTT GAATGGTTTT CCACGCCCAG AGGTCCGGCG AGT //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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