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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-Cdx2ER-IP (1)

Expression clone of mouse Cdx2-ER

Catalog number RDB10265
Resource name pPyCAG-Cdx2ER-IP (1)
Alternative name stock no 1251
Clone info. Expression clone of mouse Cdx2-ER.
Vector backbone pPyCAG-IP (Plasmid)
Size of vector backbone 6.5 kb
Selectable markers Amp^r
Gene/insert name Mouse cDNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank mouse Cdx2 (NCBI Gene 12591) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cell, 123, 917-929 (2005).).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB10265 pPyCAG-Cdx2ER-IP (1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB10265z.seq
Electronic file Map RDB10265a.gif

Original reference

original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB10265_A5Ho.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB10265_A5Hoa.seq check
>10265_10265_A5Ho_2_pAxCA_F1_A02_02.ab1
    1 CATCTTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG CCAACATGTA CGTGAGCTAC
  121 CTTCTGGACA AGGACGTGAG CATGTATCCT AGCTCCGTGC GCCACTCCGG CGGCCTGAAC
  181 CTGGCTCCGC AGAACTTTGT CAGTCCTCCG CAGTACCCGG ACTACGGTGG TTACCACGTG
  241 GCGGCCGCGG CGGCTGCTAC GGCGAACTTG GACAGCGCTC AGTCCCCAGG GCCATCCTGG
  301 CCCACCGCGT ACGGCGCCCC TCTCCGCGAG GACTGGAATG GCTACGCACC CGGGGGCGCT
  361 GCGGCAGCCA ACGCGGTAGC CCACGGTCTC AATGGTGGCT CCCCGGCCGC CGCTATGGGC
  421 TACAGCAGCC CCGCCGAATA CCACGCGCAC CATCACCCGC ATCATCACCC GCACCATCCG
  481 GCCGCCTCGC CGTCCTGCGC CTCCGGCTTG CTGCAGACGC TCAACCTCGG CCCCCCGGGG
  541 CCCGCAGCCA CCGCCGCCGC CGAACAGCTG TCCCCCAGCG GCCAGCGGCG AAACCTGTGC
  601 GAGTGGATGC GGAAGCCCGC GCAGCAGTCC CTAGAAGCCC AAGTGAAAAC CAGGACAAAG
  661 ACAAATACCC GGGTGTGTAC ACAGACCATC AGCGGCTGGA GCTGAGATGA GTTTCACTTT
  721 AGTCGATACA TCACCATCAG AGAAAAGTGA GCTGGCTGCC AACACCTTGG GCTCCTCCGA
  781 GAGCAGTAAA TTTGTTTCGA ACCGCGAGCA GAAAGAATCA GAGAAGCAGC AGCAGCAACG
  841 CAGCAGCAGC ACAAGGCTCC AAGCGCCGCC AACTCCAAGC CTAACGGGTG CCTGGCGAGC
  901 GTCGGACTGG ATCTTGTACC TCTTGCAAGG TCAGCCTGAT CTGTCTGGGG CTTCGCACTG
961 AAGTTACC
//
Primer: IRES-R
Sequence file: RDB10265_A5Hob.seq check
>10265_10265_A5Ho_2_IRES-R_B02_05.ab1
    1 GCTGGCGGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGCGGAATT
   61 CGATATCAAG CTTATCGAGC GGCCGCTCAG ATCGTGTTGG GGAAGCCCTC TGCTTCCGGG
  121 GGTATGTAGT AGGTTTGTAA GGAATGTGCT GAAGTGGAGC TGGTGGTGGC CAGCTGGGTC
  181 TGGCTGGGCT CCTCTGGGGG CACTCCCATG CGACTGGCTG GGGCATGAAG GCGGTGGGCA
  241 TCCAACATCT CCAGGAGCAG GTCATAGAGG GGTACCACGT TCTTGCATTT CATGTTGTAG
  301 AGATGCTCCA TGCGTTTGTT ACTCATATGC CGGATATGGG AAAGAATGAG AAGGAGCTGA
  361 GCTAGGCGGC GATGCTGCTG CTGCAGAGTC AGGCCAGCTT TGGCCATCAG GTGGATCAAA
  421 GTGTCTGTGA TCTTGTCCAG GACACGGTGG ATGTGGTCCT TCTCTTCCAG AGACTTCAAG
  481 GTGCTGGACA GAAACGTGTA CACTCCGGAA TTAAGCAAAA TGATGGATTT GAGGCACACA
  541 AACTCTTCAC CCTGCAGGTT CATCATGCGG AACCGACTTG ACGTAGCAAG CAACATGTCA
  601 AAGATCTCCA CCATGCCTTC CACACATTTA CCTTGATTCC TGTCCAGGAG CAAGTTAGGA
  661 GCAAACAGGA GCTTCCCCGG GTGTTCCATG GAGCGCCAGA CGAGACCAAT CATCAGAATC
  721 TCCAGCCAGG CACACTCGAG AAGGTGGACC TGATCATGGA GATTCAAGTC CCCAAAGCCT
  781 GGCACTCTCT TTGCCCAGTT GATCATATGA ACCAGCTCCC TATCTGCTAG GTTGGTCAAT
  841 AAGCCCATCA TTGAGGCTTC ACTGAAGGGT CTAGAAGGAT CATATTCAGA ATAGATCATG
  901 GGCGGGTTCA GCATCCAACA AGGCACTGAC CATCTGGGTC AGCTGTCAAG GGACAGGCAG
  961 GGCTATTCTT CTTAGTGTGC TTTATTCACA GAGGCTTGCC AAGTTGGCAG CCTCATGTCT
 1021 CTGAAGCACC CAATTCCATT TCGACTAGTC TGAGTGACTG AGAGTTAAAA CCCCCTCCAG
1081 CCTGGTCCTC CGA
//
Primer: Cdx2ER-M_F
Sequence file: RDB10265_A5Hoc.seq check
>10265_10265_A5Ho_2_CdxER-M_F_C01_07.ab1
    1 GCAGACATCA GCGGCTGGAG CTGGAGAGGA GTTTCACTTT AGTCGATACA TCACCATCAG
   61 GAGGAAAAGT GAGCTGGCTG CCACACTTGG GCTCTCCGAG AGGCAGGTTA AAATTTGGTT
  121 TCAGAACCGC AGAGCCAAGG AGAGGAAAAT CAAGAAGAAG CAGCAGCAGC AACAGCAGCA
  181 GCAGCAACAA CAGCCTCCAC AGCCGCCGCC ACAACCTTCC CAGCCTCAGC CGGGTGCCCT
  241 GCGGAGCGTG CCCGAGCCCT TGAGTCCTGT GACCTCCTTG CAAGGCTCAG TGCCTGGTTC
  301 TGTCCCTGGG GTTCTGGGGC CAGCTGGAGG GGTTTTAAAC TCCTCAGTCA CCCAGACTAG
  361 TCGAAATGAA ATGGGTGCTT CAGGAGACAT GAGGGCTGCC AACCTTTGGC CAAGCCCTCT
  421 TGTGATTAAG CACACTAAGA AGAATAGCCC TGCCTTGTCC TTGACAGCTG ACCAGATGGT
  481 CAGTGCCTTG TTGGATGCTG AACCGCCCAT GATCTATTCT GAATATGATC CTTCTAGACC
  541 CTTCAGTGAA GCCTCAATGA TGGGCTTATT GACCAACCTA GCAGATAGGG AGCTGGTTCA
  601 TATGATCAAC TGGGCAAAGA GAGTGCCAGG CTTTGGGGAC TTGAATCTCC ATGATCAGGT
  661 CCACCTTCTC GAGTGTGCCT GGCTGGAGAT TCTGATGATT GGTCTCGTCT GGCGCTCCAT
  721 GGAACACCCG GGGAAGCTCC TGTTTGCTCC TAACTTGCTC CTGGACAGGA ATCAAGGTAA
  781 ATGTGTGGGA AGGCATGGTG GAGATCTTTG ACATGTTGCT TGCTACGTCA AGTCGGTTCC
  841 GCATGATGAA CCTGCAGGTG AAGAGTTTTG TGTGCCTCAA ATCCATCATT TTGCTTAATT
  901 TCCCGGAGTG TACACGTTTC TGTCCAGCCA CCCTGAAGTC TCTGGAAGAG AAGACCCACA
  961 TCCACCCGTG TCTGAAACAA GATCACAGGA ACACCTTGAT CAACTGATTG CCTAGCTGAC
 1021 TGACTTGCAG CGAGCAGCAT CGCGGCTAGC TCAAGCTCTC CGATCTATGC CGATAATCAC
//
Primer: IRES-F
Sequence file: RDB10265_A5Hod.seq check
>10265_10265_A5Ho_2_IRES-F_C02_08.ab1
    1 ACATCATTCT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGACCGA GTACAAGCCC
  121 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG
  181 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
  241 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
  301 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
  361 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
  421 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
  481 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
  541 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
  601 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
  661 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG
  721 CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATCTAGAG CTCGCTGATC
  781 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCCTT
  841 CCTTGACCCT GGAAAGGTGC CACTCCCCAC TGTCCTTTCC TAATAAAAAT GAGAAATGCA
  901 TCGCATGTCT GAGTAGGTGT CATTCTATTC TGCGATGGTT GGCAGGACAG CCAAGGGGAG
  961 ATGGGAAGAC AATAGCAGCA TGCTGGGGAT GCGGTGCTCT ATGCCTCTGA AGGCGAAGAC
1021 CTGCAGTCAG CTTGGCACTA CAGCCTATAG CGATCGGGGG AATGTTAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl