Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmPGKpacECFPpA (#RDB10118)

Expression vector of pac-ECFP with mPGK promoter.

Alternative name	stock no 1095
Clone info.	Expression vector of pac-ECFP with mPGK promoter and poly(A).
Vector backbone	pBIKS(-) (Plasmid)
Selectable markers	Ampicillin (E. coli), puromycin (mammalian cell)
Depositor|Developer	Niwa, Hitoshi |

 

Remarks, protocol and/or map (pdf)	RDB10118p.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB10118	pmPGKpacECFPpA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pmPGKpacECFPpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10118).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB10118_B3E9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308) Region: T7 pro,insert 5'(PGK pro) Sequence file: RDB10118_B3E9a.seq
>10118_10118_B3E9_3_f1ori_F2_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTN NNGNNTTCGN NCTATTGGTT AAAAAATGAG CTGANTTAAC    61 AAAAATTTNN NNNNNNNNTT ANCAAAATAT TAACGCTTAC AATTTCCATT CNNNATTCAG   121 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC   181 GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG   241 ACGTTGTAAA ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA   301 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCTA   361 CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT   421 GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG   481 GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT   541 CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC   601 ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT   661 TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG   721 GGGTGGGTCC GGGGGCGGGC TCANGGGCGG GCTCANGGGC GGGGCGGGCG CCCGAANGTC   781 CTCCGGANGC CCGGCATTCT GCACGCTTCA AAANCGCACG TCTGCCGCGC TGTTCTCCTC   841 TTNCTCATCT CCNGGCNTTT CNANCGANCA TCNAGCTNGA TCNTCANGAN CGANNACNAG   901 CCCNCNGNGN NNCNNNNNAC CCNNNANNAN NTCCCCAGGG NCNNNACNNN CCNNCNNNGN 961 NNNNNTNNNN CNNANNNNCC NNNNNNNNNN //
Primer: SV40pA3element Reverse (Pr0108) Region: insert 3'(PGK pA) Sequence file: RDB10118_B3E9b.seq
>10118_10118_B3E9_3_SV40pA3'element Reverse_A01_01_ABI24.ab1     1 NNNNNNNNNN NNGNGNNNNN ATNNNNNNNC NNGCAACGCG GCCTTTNNNN NNTTCCTGGC    61 CTTTTGCNNN NNCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA   121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA   181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC   241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG   301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA   361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA   421 GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG   481 GTACCGGGCC CCCCCTCGAG GTCGACGGAT CCAGATCTAT AGATCATGAG TGGGAGGAAT   541 GAGCTGGCCC TTAATTTGGT TTTGCTTGTT TAAATTATGA TATCCAACTA TGAAACATTA   601 TCATAAAGCA ATAGTAAAGA GCCTTCAGTA AAGAGCAGGC ATTTATCTAA TCCCACCCCA   661 CCCCCACCCC CGTAGCTCCA ATCCTTCCAT TCAAAATGTA NGTACTCTGT TCTCACCCTT   721 CTTAACAAAG TATGACAGGA AAAACTTCCA TGTGGACATC TTTATTGTTT AATAGATCAT   781 CAATTTCTCG ACTATTCCTT TGCCCTCNGA CGANTGCTGG GGCGTCNNNT TCCACTATCG   841 GCGANTCGAT AANCTGGCCG CTTTANTTGN ACAGCNCGNC CATGCCGANN GNGNTNCNNG   901 NNGNNNGNNN NNANNNNNNN CNNNANCNNN NNNTCNCNNN TNNNCNNNGG GGNCTTNNNN 961 NNNGNNNGNN NNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(PuroR 3',ECFP 5') Sequence file: RDB10118_B3E9c.seq
>10118_10118_B3E9_3_PGKpro_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCN NGCCTTTCGA CCGNTCATCN AGCTTGATCC    61 TCATGACCGA GTNNCNANNC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC   121 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG   181 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC   241 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG   301 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC   361 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG   421 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG   481 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC   541 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC   601 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC   661 GGATCGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG   721 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGANCTGG ACGGCGACGT AAACGGCCAC   781 AAGTTCAGCG TGTCCGGCGA GGGCGANGGC GANGCCNCCT ACNGCAAGCT GANCCTGNAN   841 TTCNTCTGCA CCACCGGCAA GCTGCCCGNG CCCNNNNCCA CCNNCNNGNN CNCCNNNNNN   901 NGGGGNGNGC ANNGNTTCNG CCNCTACCCN NANNNCNTGN ANCNNCNCNA NNTNNNTNNN 961 NNNNNNN //
Primer: T3 (Pr0112) Region: insert mid(PGK pA,ECFP 3') Sequence file: RDB10118_B3E9d.seq
>10118_10118_B3E9_3_T3_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNCCNNN NNNGNCGNNN GNNCNNGATC TATAGATCAT GAGTGGGAGG    61 AATGAGCTGG NCCNNNNNTT GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA   121 TTATCATAAA GCAATAGTAA AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC   181 CCACCCCCAC CCCCGTAGCT CCAATCCTTC CATTCAAAAT GTAGGTACTC TGTTCTCACC   241 CTTCTTAACA AAGTATGACA GGAAAAACTT CCATGTGGAC ATCTTTATTG TTTAATAGAT   301 CATCAATTTC TCGACTATTC CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA   361 TCGGCGAGTC GATAAGCTGG CCGCTTTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC   421 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC   481 TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA   541 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC   601 GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT   661 GGCTGATGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA   721 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGNG   781 TCTTGTAGTT GCCGTCGNCC TTGAAGAANA NGGTGCGCTC CTGGNCGTAN CCTTCNGGCN   841 TGGCGGANTN GAAGAANTCG NGCTGCTTCN TGTGGNCNGG GNANCGGCNN ANNCACTGCA 901 CGCCCCANNN CNNNNNNNNA CNNNGNNGGG CNANGGNNNG GNNNCTNGNN NNNNNNNN //
Primer: SK (Pr0103) Region: insert mid(PGK pro,PuroR 5') Sequence file: RDB10118_B3E9e.seq
>10118_10118_B3E9_3_SK_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GGNANGGGNN GNGCTTTTCC NNAGGNAGTC TGGAGCATGC    61 GCTTTAGCAG CCCCNNTGGG NACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT   121 TCCACATCCA CCGGTAGGCG CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT   181 TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG   241 TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA   301 TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG   361 GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG   421 GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT   481 GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA CCGATCATCA AGCTTGATCC   541 TCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG   601 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG   661 ANCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG   721 ACATCGGCAA GGNGTGGGTC GCGNANGANG GCGCCGCGGN GGCGGTCTGG NNCACGCCGG   781 ANAGCGTCNA ANCGGGGGCG GNGTTCGCCG ANNTCGGNCC GCGCATGNNC NANTTGANCN   841 GNNNNCCGGN TGNNCNNNNA NCNNCNGATG GNANNNCNNN CNNNNNNNNN ANNNGNCCCN 901 ANGNANCNNN NNNNNNN //
Primer: PuroR_F (Pr0554) Region: insert mid(ECFP 5') Sequence file: RDB10118_B3E9f.seq
>10118_10118_B3E9_3_PuroR_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNCNNNNNN NCNGNNNGNN CGCGCACCTG GTGCATGACC CGCAAGCCCG    61 GTGNCNNNNN CNNNNGTACC GCGGGCCCGG GNTCCACCGG TCGCCACCAT GGTGAGCAAG   121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC   181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC   241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC   301 CTGACCTGGG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC   421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC   481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC   541 AACTACATCA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC   601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG   661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGANCACC   721 CAGTCCGCCC TGANCAAAGA NCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC   781 GTGACCGCCG CCNGGNTCAC TCTCGGCATG GNNGANCTGT ACNAGTAAAG CGGCCAGCTT   841 ATCGACTCGC CNATANNGGA AACCGNNGCC CCANCACTCN NNCNANGGCA AAGGNNNANN   901 CNANAANNTG ATGANCTANT NNANNNNNAA NANGNCCNNN NNGNNANNTT TTNNNNNNNN 961 NN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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