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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBlKS-Cre

Plasmid clone of Cre.

Catalog number RDB09951
Resource name pBlKS-Cre
Alternative name stock no 915
Clone info. Plasmid clone of Cre.
Vector backbone pBluescript KS(+) (Plasmid)
Selectable markers Ampicillin
Gene/insert name P1 phage Cre recombinase cDNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank P1 phage cre |

Distribution information

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RDB09951 pBlKS-Cre DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Remarks, protocol and/or map (pdf) RDB09951_pBlKS-Cre.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09951_B0Esp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13R
Region: KpnI, Cre 5'
Sequence file: RDB09951_B0Esa.seq check
>09951_09951_B0Es_3_M13R_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNAAGGGNA NNAAAGCTGG TACCGGGCCC CCCCTCGAGG
   61 GGCAGAGCCG ATCCTGTACA CTTTACTTAA AACCATTATC TGAGTGTGAA ATGTCCAATT
  121 TACTGACCGT ACACCAAAAT TTGCCTGCAT TACCGGTCGA TGCAACGAGT GATGAGGTTC
  181 GCAAGAACCT GATGGACATG TTCAGGGATC GCCAGGCGTT TTCTGAGCAT ACCTGGAAAA
  241 TGCTTCTGTC CGTTTGCCGG TCGTGGGCGG CATGGTGCAA GTTGAATAAC CGGAAATGGT
  301 TTCCCGCAGA ACCTGAAGAT GTTCGCGATT ATCTTCTATA TCTTCAGGCG CGCGGTCTGG
  361 CAGTAAAAAC TATCCAGCAA CATTTGGGCC AGCTAAACAT GCTTCATCGT CGGTCCGGGC
  421 TGCCACGACC AAGTGACAGC AATGCTGTTT CACTGGTTAT GCGGCGGATC CGAAAAGAAA
  481 ACGTTGATGC CGGTGAACGT GCAAAACAGG CTCTAGCGTT CGAACGCACT GATTTCGACC
  541 AGGTTCGTTC ACTCATGGAA AATAGCGATC GCTGCCAGGA TATACGTAAT CTGGCATTTC
  601 TGGGGATTGC TTATAACACC CTGTTACGTA TAGCCGAAAT TGCCAGGATC AGGGTTAAAG
  661 ATATCTCACG TACTGACGGT GGGAGAATGT TAATCCATAT TGGCAGAACG AAAACGCTGG
  721 TTAGCACCGC ANGTGTAGAG AAGGCACTTA GCCTGGGGGT AACTAAACTG GTCGAGCGAT
  781 GGATTTCCGT CTCTGGTGTA GCTGATGATC CGAATAACTA CCNGTTTTGC CGGGNCAGAA
  841 AAAATGGTGT TGCCGCGCCA TCTGCCNCCA GCCAGCTATC AACTCGCGCC CTNNNNNGNA
  901 NTTTTGAANC AACTCATCGA NNGATTTACN GCNCTNANGA TGANTCNNGN NCAGANATAC
961 CNNNNNTGGN NNGGNNACAN NNNNNGNNNN NNNNNN
//
Primer: pUC-M13_-100
Region: T7pro, SacI, Cre 3'
Sequence file: RDB09951_B0Esb.seq check
>09951_09951_B0Es_3_pUC-M13_-100_C07_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCNGNNTTTT CCCNGNCNCN NACGTTGTAA
   61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCCACCGCGG
  121 TGGCGGCCGC TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGCGT TAATGGCTAA
  181 TCGCCATCTT CCAGCAGGCG CACCATTGCC CCTGTTTCAC TATCCAGGTT ACGGATATAG
  241 TTCATGACAA TATTTACATT GGTCCAGCCA CCAGCTTGCA TGATCTCCGG TATTGAAACT
  301 CCAGCGCGGG CCATATCTCG CGCGGCTCCG ACACGGGCAC TGTGTCCAGA CCAGGCCAGG
  361 TATCTCTGAC CAGAGTCATC CTTAGCGCCG TAAATCAATC GATGAGTTGC TTCAAAAATC
  421 CCTTCCAGGG CGCGAGTTGA TAGCTGGCTG GTGGCAGATG GCGCGGCAAC ACCATTTTTT
  481 CTGACCCGGC AAAACAGGTA GTTATTCGGA TCATCAGCTA CACCAGAGAC GGAAATCCAT
  541 CGCTCGACCA GTTTAGTTAC CCCCAGGCTA AGTGCCTTCT CTACACCTGC GGTGCTAACC
  601 AGCGTTTTCG TTCTGCCAAT ATGGATTAAC ATTCTCCCAC CGTCAGTACG TGAGATATCT
  661 TTAACCCTGA TCCTGGCAAT TTCGGCTATA CGTAACAGGG TGTTATAAGC AATCCCCAGA
  721 AATGCCAGAT TACGTATATC CTGGCAGCGA TCGCTATTTT CCATGAGTGA ACGAACCTGG
  781 TCGAAATCAG TGCGTTCGAN CGCTAGAGCC TGTTTTGCAC NTTCACCGGC ATCANCGTTT
  841 TCTTTTCGGA TNCGCCGCAT ANNNNGNGAA ACAGCATTGC TGTCNCTNGN NCNNNGCANC
  901 CCGGANCNAN NATGANNCNT GTTTANCNNG NCNNAANGNN NNTGGANANT TTTTNNTNNN
961 NNANCNNNNG CNNGAANANN NNNNNNANNN NCGCGNNCNT CNTNNNNNNN NNN
//
Primer: Cre-N_R
Region: Cre 5', KpnI, T3pro, lacO, lacpro, CAPsite, ori
Sequence file: RDB09951_B0Esc.seq check
>09951_09951_B0Es_3_Cre-N_R_C09_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNGNGNNCC NTGNNNNTGT CCNTCANNGT TCTTGCGAAC
   61 CTCATCACTC GTTGCATCGA CCGGTAATGC AGGCAAATTT TGGTGTACGG TCAGTAAATT
  121 GGACATTTCA CACTCAGATA ATGGTTTTAA GTAAAGTGTA CAGGATCGGC TCTGCCCCTC
  181 GAGGGGGGGC CCGGTACCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTTC GAGCTTGGCG
  241 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  301 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
  361 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
  421 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC
  481 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
  541 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
  601 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
  661 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
  721 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
  781 CCGACCCTGC CGCTTACCGG ATACCTGNCC GCCNNNCTCC CTTCGGGAAN CGNGGCGCTT
  841 TCTCATANCT CACGCTGTAN GTATCTCNGT TCGGNNTAGG NCGTTCGNNN CAANCTGGGC
  901 TGTGTGCNCN AACCCCCNNN NNNANCCNGA NNGCNNNNCC NTATNCNGNN AACTNNNGNN
961 NNGANNNNNA CCNNGNNNNN ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2020.10.02

GNP_filter3_RDBDEP_html_200316.pl