Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAGGSneo deltaEcoRI (#RDB09694)

Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker.

Alternative name	pCAGGSneodelEcoRI; stock no 650
Clone info.	Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori); polyoma enhancer/herpes simplex virus thymidine kinase (MC1) promoter neo marker.
Vector backbone	Plasmid
Depositor|Developer	Niwa, Hitoshi |

 

Remarks, protocol and/or map (pdf)	RDB09694p.pdf

Distribution information

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB09694	pCAGGSneo deltaEcoRI	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCAGGSneo deltaEcoRI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09694).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB09694_A4Hep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: beta-globin pA Sequence file: RDB09694_A4Hea.seq
>09694_09694_B1IaF_1_pAxCA_F1_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNNNNNNC NNNCTCATCA TTTTGGCAAA GAATTCCTCG    61 AGGAATTCAC TCCTCAGGTG CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG   121 CCCTGGCTCA CAAATACCAC TGAGATCTTT TTCCCTCTGC CAAAAATTAT GGGGACATCA   181 TGAAGCCCCT TGAGCATCTG ACTTCTGGCT AATAAAGGAA ATTTATTTTC ATTGCAATAG   241 TGTGTTGGAA TTTTTTGTGT CTCTCACTCG GAAGGACATA TGGGAGGGCA AATCATTTAA   301 AACATCAGAA TGAGTATTTG GTTTANAGTT TGGCAACATA TGCCCATATG CTGGCTGCCA   361 TGAACAAANN NNNNCTATAA AGAGGTCATC AGTATATGAA ACAGCCCCCT GCTGTCCATT   421 CCTTATTCCA TANAAAAGCC TNNNNNNGAG GTTAGATTTT TTTTATATTT TGTTTTGTGT   481 TATTTTTTTC TTTAACATCC CTAAAATTTT CCTTACATGT TTTACTAGCC AGANTTTTCC   541 TCCTCTCCTG NNNTACTCCC ANTCATAGCT GTNCCTCTTC NNNNNANNNN NATCCCTNNA   601 CCTGNNNNNC NAANCNTNNN NNNANNCATG GNCNNNNNTN NTTCNNGTGT GAANNNNTTN 661 NCNGCTNNNA NNNCNNNNNA CNNANNNNNC NGNAANNNNN NNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,polyoma virus en,MC1 pro,NeoR 5' Sequence file: RDB09694_A4Heb.seq
>09694_09694_A9E9F_Amp_R_A05_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NAGCNNTTAT NNGGGTTATT GTCTCATGAG CGGANACATA    61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG   121 CCACCTGGGT CGAGCAGTGT GGTTTTCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT   181 GGAATGTTTC CACCCAATGT CGAGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC   241 CACCCAGGCC TGGAATGTTT CCACCCAATG TCGAGCAAAC CCCGCCCAGC GTCTTGTCAT   301 TGGCGAATTA ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG   361 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCCA ATATGGGATC   421 GGCCATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT   481 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC   541 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT   601 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT   661 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA   721 GGATCTCCTG TCATNNNNNN TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT   781 GCGGCGGCTG CNTACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCNAN NNAAACATCG   841 CATCNNAGCG AGCACGTNCT CNGGATGGNA ANCCGGTCTT GTCGATCANG ATGATNTGGN   901 NNANNNNCNN CANGGGCTCN CNNCNNNCNN AANNNGNNCN NNNGGCTNNN NGNNCGCANG   961 NCCCNANNGC NNNNNTNNNN TNNNNGNCCC NNTNGNNNAN GNCTGCNTNG NCNAANNNNN 1021 NNNGNNNGNA AANNNGNNCN NNNTTNNNNG NANTNNNNNN NNNN //
Primer: Neo-C_F (Pr0148) Region: NeoR,MC1 pA,CMV en Sequence file: RDB09694_A4Hec.seq
>09694_09694_B1IlF_Neo-C_F_G02_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC    61 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGGGGATCGG CAATAAAAAG   121 ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCGGAT CCGTCGACAT TGATTATTGA   181 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC   241 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT   301 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC   361 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC   421 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT   481 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA   541 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCNN NNTCTCCCCC CCCTCCCCNN   601 CCCCAATTTT GNATTTATTT ANTTTTTNAN NTATTTTGNG CNNCNANNNN GGNCNNNNNN 661 NNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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