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pCAGGSneo deltaEcoRI

Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker.

Catalog number RDB09694
Resource name pCAGGSneo deltaEcoRI
Alternative name pCAGGSneodelEcoRI; stock no 650
Clone info. Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori); polyoma enhancer/herpes simplex virus thymidine kinase (MC1) promoter neo marker.
Vector backbone Plasmid
Depositor|Developer Niwa, Hitoshi |
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            RDB09694 pCAGGSneo deltaEcoRI DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB09694p.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09694_A4Hep1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Region: beta-globin pA
            Sequence file: RDB09694_A4Hea.seq check
            >09694_09694_B1IaF_1_pAxCA_F1_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNTNNNNNNC NNNCTCATCA TTTTGGCAAA GAATTCCTCG
               61 AGGAATTCAC TCCTCAGGTG CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG
              121 CCCTGGCTCA CAAATACCAC TGAGATCTTT TTCCCTCTGC CAAAAATTAT GGGGACATCA
              181 TGAAGCCCCT TGAGCATCTG ACTTCTGGCT AATAAAGGAA ATTTATTTTC ATTGCAATAG
              241 TGTGTTGGAA TTTTTTGTGT CTCTCACTCG GAAGGACATA TGGGAGGGCA AATCATTTAA
              301 AACATCAGAA TGAGTATTTG GTTTANAGTT TGGCAACATA TGCCCATATG CTGGCTGCCA
              361 TGAACAAANN NNNNCTATAA AGAGGTCATC AGTATATGAA ACAGCCCCCT GCTGTCCATT
              421 CCTTATTCCA TANAAAAGCC TNNNNNNGAG GTTAGATTTT TTTTATATTT TGTTTTGTGT
              481 TATTTTTTTC TTTAACATCC CTAAAATTTT CCTTACATGT TTTACTAGCC AGANTTTTCC
              541 TCCTCTCCTG NNNTACTCCC ANTCATAGCT GTNCCTCTTC NNNNNANNNN NATCCCTNNA
              601 CCTGNNNNNC NAANCNTNNN NNNANNCATG GNCNNNNNTN NTTCNNGTGT GAANNNNTTN
            661 NCNGCTNNNA NNNCNNNNNA CNNANNNNNC NGNAANNNNN NNNNN
            //
            Primer: Amp_R
            Region: AmpR pro,polyoma virus en,MC1 pro,NeoR 5'
            Sequence file: RDB09694_A4Heb.seq check
            >09694_09694_A9E9F_Amp_R_A05_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNG NAGCNNTTAT NNGGGTTATT GTCTCATGAG CGGANACATA
               61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
              121 CCACCTGGGT CGAGCAGTGT GGTTTTCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT
              181 GGAATGTTTC CACCCAATGT CGAGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC
              241 CACCCAGGCC TGGAATGTTT CCACCCAATG TCGAGCAAAC CCCGCCCAGC GTCTTGTCAT
              301 TGGCGAATTA ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG
              361 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCCA ATATGGGATC
              421 GGCCATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
              481 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
              541 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
              601 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
              661 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
              721 GGATCTCCTG TCATNNNNNN TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
              781 GCGGCGGCTG CNTACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCNAN NNAAACATCG
              841 CATCNNAGCG AGCACGTNCT CNGGATGGNA ANCCGGTCTT GTCGATCANG ATGATNTGGN
              901 NNANNNNCNN CANGGGCTCN CNNCNNNCNN AANNNGNNCN NNNGGCTNNN NGNNCGCANG
              961 NCCCNANNGC NNNNNTNNNN TNNNNGNCCC NNTNGNNNAN GNCTGCNTNG NCNAANNNNN
            1021 NNNGNNNGNA AANNNGNNCN NNNTTNNNNG NANTNNNNNN NNNN
            //
            Primer: Neo-C_F
            Region: NeoR,MC1 pA,CMV en
            Sequence file: RDB09694_A4Hec.seq check
            >09694_09694_B1IlF_Neo-C_F_G02_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
               61 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGGGGATCGG CAATAAAAAG
              121 ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCGGAT CCGTCGACAT TGATTATTGA
              181 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC
              241 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT
              301 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC
              361 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC
              421 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT
              481 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA
              541 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCNN NNTCTCCCCC CCCTCCCCNN
              601 CCCCAATTTT GNATTTATTT ANTTTTTNAN NTATTTTGNG CNNCNANNNN GGNCNNNNNN
            661 NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl