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pPyCAG-Cre-IRESpuropA

Expression vector of Cre recombinase. Puromycin resistance.

Catalog number RDB09596
Resource name pPyCAG-Cre-IRESpuropA
Alternative name pPyCAG-Cre-IP; stock no 547
Clone info. Expression vector of Cre recombinase. Puromycin resistance.
Vector backbone Plasmid
Gene/insert name P1 phage Cre recombinase genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank P1 phage cre |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999).
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB09596 pPyCAG-Cre-IRESpuropA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB09596z.seq
Electronic file Map RDB09596a.gif

Original reference

original Tashiro, F., Constructing adenoviral vectors by using the circular form of the adenoviral genome cloned in a cosmid and the Cre-loxP recombination system. Hum. Gene Ther. 10 (11): 1845-1852 (1999). PMID 10446924.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)
Featured content Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09596_A7Bdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: Cre 5'
Sequence file: RDB09596_A7Bda.seq check
>09596_09596_A7Bd_pAxCA_F1_H02_08_ABI08.ab1
    1 CATTCCTTGC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTCGAG GGGCAGAGCC GATCCTGTAC
  121 ACTTTACTTA AAACCATTAT CTGAGTGTGA AATGTCCAAT TTACTGACCG TACACCAAAA
  181 TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT CGCAAGAACC TGATGGACAT
  241 GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT CCGTTTGCCG
  301 GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG TTTCCCGCAG AACCTGAAGA
  361 TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG GCAGTAAAAA CTATCCAGCA
  421 ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG CTGCCACGAC CAAGTGACAG
  481 CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA AACGTTGATG CCGGTGAACG
  541 TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC CAGGTTCGTT CACTCATGGA
  601 AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT CTGGGGATTG CTTATAACAC
  661 CCTGTTACGT ATAGCCGAAA TTGCCAGGAT CAGGGTTAAA GATATCTCAC GTACTGACGG
  721 TGGGAGAATG TTAATCCATA TTGGCAGAAC GAAAACGCTG GTTAGCACCG CAGTGTAGAG
  781 AAGGCACTTA GCCTGGGGGT AACTAAACTG GTCGAGCGAT GGATTTCCGT CTCTGGTGTA
  841 GCTGATGATC CGAATAACTA CCTGTTTTGC CCGGGTCAGA AAAAATGGTG TTGCCGCGCC
  901 ATCTGCCACC AGCCCAGCTA TCAACTCGCG CCCTGAAGGG ATTTTTGAAG CAACTCATCG
  961 ATTGGATTAC GGCGCTAGAT GACCTCTGGT CAGAGAATAC CTGCTGATCT GGACACATTA
 1021 CCCGTGTCGG ACCGCCGCGA GAATATGGCC CGGCCTGGAG TTCTAATCGA ATCATGCAGC
1081 TGGGTGCCTG GAACCAATGT GTA
//
Primer: IRES-R
Region: IRES2, Cre 3'
Sequence file: RDB09596_A7Bdb.seq check
>09596_09596_A7Bd_IRES-R_F02_06_ABI08.ab1
    1 GGCTCGCGGG CTTCGGCCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGCGGA
   61 ATTCGATATC AAGCTTATCG AGCGGCCGAT CTCAGTGGTA TTTGTGAGCC AGGGCATTGG
  121 CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT TCGCGTTAAT
  181 GGCTAATCGC CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG
  241 ATATAGTTCA TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT
  301 GAAACTCCAG CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG
  361 GCCAGGTATC TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA
  421 AAAATCCCTT CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA
  481 TTTTTTCTGA CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA
  541 ATCCATCGCT CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG
  601 CTAACCAGCG TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG
  661 ATATCTTTAA CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAAGCAAT
  721 CCCCAGAAAA TGCCAGATTA CGGTATATCC TGGCAGCGAT CGCTATTTTT CCATGAGTGA
  781 ACGAACCTGG GTCGAAATCA GTGCGTTTCG AACGGCTAGA GCCTGTTTTG CACGTTCACC
  841 GGCAATCAAC GTTTTTCTTT TCGGATCCCG CCGCATAACC CAGGTGAAAC AGCAATTTGC
  901 CTGTCACTTT GGTTCGTGGC CAGCCCCGGA ACCGGACGAT GAAAGCCATG TTTTAGCCTG
  961 GGCCCCAAAT GTTTGCCTGG GAATAGTTTT TTACTGGCCA GGACCGCCGC GCCTTGAAGA
 1021 ATTATAGGAG AGAATAATTC GCGAAACCAT TCTTTCCAGG GTTCGTGCCG GGGAAAACCA
 1081 ATATCCCGGG TTTATTTCCA ACTTTGCCAC CCAATGCCCG GTCCCAACGC GAAACTCGCG
1141 GGGGCACAGA CGACG
//
Primer: IRES-F
Region: IRES2, PuroR, BGH p(A)
Sequence file: RDB09596_A7Bdc.seq check
>09596_09596_A7Bd_IRES-F_E02_05_ABI08.ab1
    1 GCATGCTTCA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGTTGG GTTGGGGCAG GACAGCCAAG
  961 GGGGGGAGGG ATTTGGGAAG AACAATTAGG CAGGCATGGC TTGGGAATGC CGTTGGGCCT
1021 ATATTGGCCT TCTGTAGACC GGGAAAGGAA ACTGCTGCTG TC
//
Primer: Amp_R
Region: Polyoma Ori, SV40 p(A), HCMVIEE
Sequence file: RDB09596_A7Bdd.seq check
>09596_09596_A7Bd_Amp_R_F05_17_ABI24.ab1
    1 CTTCATTTCA TGACCGCTTT GGAAACAAGA CTGTATTTCC TGGAAATTAA TGTTTATTCA
   61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG
  121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC
  181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA
  241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG
  301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA
  361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG
  421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT
  481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC
  541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC
  601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG
  721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT
  781 AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG
  841 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC
  901 GCCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  961 TTGGCAGTAC ATCAAGTGTA TCATATGCCA GGTACGCCCC CTATTGACGT CATGAACGTA
 1021 AATGACCGCT GCATTATGCC AGTTACATGA CGTATGGACT TTACGTACTG GCAGTTACAT
1081 CTTACGTATA GATCAATCGC TAATTACCAA T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tashiro, F., Constructing adenoviral vectors by using the circular form of the adenoviral genome cloned in a cosmid and the Cre-loxP recombination system. Hum. Gene Ther. 10 (11): 1845-1852 (1999). PMID 10446924.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl