Expression vector of Cre recombinase. Puromycin resistance.
| Catalog number | RDB09596 |
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| Resource name | pPyCAG-Cre-IRESpuropA |
| Alternative name | pPyCAG-Cre-IP; stock no 547 |
| Clone info. | Expression vector of Cre recombinase. Puromycin resistance. |
| Vector backbone | Plasmid |
| Gene/insert name | P1 phage Cre recombinase genomic DNA |
| Depositor | Niwa, Hitoshi | |
| Other clones in our bank | P1 phage cre | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB09596 | pPyCAG-Cre-IRESpuropA | DNA solution |
Electronic file
| Electronic file | Sequence RDB09596z.seq from Depositor |
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| Electronic file | Map RDB09596a.gif from Depositor
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Featured content
| Featured content | Cre-loxP and FLP-FRT System (English text) |
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References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB09596_A7Bdp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB09596_A7Bda.seq |
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>09596_09596_A7Bd_pAxCA_F1_H02_08_ABI08.ab1 1 CATTCCTTGC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG 61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTCGAG GGGCAGAGCC GATCCTGTAC 121 ACTTTACTTA AAACCATTAT CTGAGTGTGA AATGTCCAAT TTACTGACCG TACACCAAAA 181 TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT CGCAAGAACC TGATGGACAT 241 GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT CCGTTTGCCG 301 GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG TTTCCCGCAG AACCTGAAGA 361 TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG GCAGTAAAAA CTATCCAGCA 421 ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG CTGCCACGAC CAAGTGACAG 481 CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA AACGTTGATG CCGGTGAACG 541 TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC CAGGTTCGTT CACTCATGGA 601 AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT CTGGGGATTG CTTATAACAC 661 CCTGTTACGT ATAGCCGAAA TTGCCAGGAT CAGGGTTAAA GATATCTCAC GTACTGACGG 721 TGGGAGAATG TTAATCCATA TTGGCAGAAC GAAAACGCTG GTTAGCACCG CAGTGTAGAG 781 AAGGCACTTA GCCTGGGGGT AACTAAACTG GTCGAGCGAT GGATTTCCGT CTCTGGTGTA 841 GCTGATGATC CGAATAACTA CCTGTTTTGC CCGGGTCAGA AAAAATGGTG TTGCCGCGCC 901 ATCTGCCACC AGCCCAGCTA TCAACTCGCG CCCTGAAGGG ATTTTTGAAG CAACTCATCG 961 ATTGGATTAC GGCGCTAGAT GACCTCTGGT CAGAGAATAC CTGCTGATCT GGACACATTA 1021 CCCGTGTCGG ACCGCCGCGA GAATATGGCC CGGCCTGGAG TTCTAATCGA ATCATGCAGC 1081 TGGGTGCCTG GAACCAATGT GTA // |
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| Primer: IRES-R | RDB09596_A7Bdb.seq |
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>09596_09596_A7Bd_IRES-R_F02_06_ABI08.ab1 1 GGCTCGCGGG CTTCGGCCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGCGGA 61 ATTCGATATC AAGCTTATCG AGCGGCCGAT CTCAGTGGTA TTTGTGAGCC AGGGCATTGG 121 CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT TCGCGTTAAT 181 GGCTAATCGC CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG 241 ATATAGTTCA TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT 301 GAAACTCCAG CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG 361 GCCAGGTATC TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA 421 AAAATCCCTT CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA 481 TTTTTTCTGA CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA 541 ATCCATCGCT CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG 601 CTAACCAGCG TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG 661 ATATCTTTAA CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAAGCAAT 721 CCCCAGAAAA TGCCAGATTA CGGTATATCC TGGCAGCGAT CGCTATTTTT CCATGAGTGA 781 ACGAACCTGG GTCGAAATCA GTGCGTTTCG AACGGCTAGA GCCTGTTTTG CACGTTCACC 841 GGCAATCAAC GTTTTTCTTT TCGGATCCCG CCGCATAACC CAGGTGAAAC AGCAATTTGC 901 CTGTCACTTT GGTTCGTGGC CAGCCCCGGA ACCGGACGAT GAAAGCCATG TTTTAGCCTG 961 GGCCCCAAAT GTTTGCCTGG GAATAGTTTT TTACTGGCCA GGACCGCCGC GCCTTGAAGA 1021 ATTATAGGAG AGAATAATTC GCGAAACCAT TCTTTCCAGG GTTCGTGCCG GGGAAAACCA 1081 ATATCCCGGG TTTATTTCCA ACTTTGCCAC CCAATGCCCG GTCCCAACGC GAAACTCGCG 1141 GGGGCACAGA CGACG // |
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| Primer: IRES-F | RDB09596_A7Bdc.seq |
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>09596_09596_A7Bd_IRES-F_E02_05_ABI08.ab1 1 GCATGCTTCA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC 61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC 121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT 241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC 361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA 421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC 481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC 541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG 601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA 661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC 721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT 781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT 841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT 901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGTTGG GTTGGGGCAG GACAGCCAAG 961 GGGGGGAGGG ATTTGGGAAG AACAATTAGG CAGGCATGGC TTGGGAATGC CGTTGGGCCT 1021 ATATTGGCCT TCTGTAGACC GGGAAAGGAA ACTGCTGCTG TC // |
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| Primer: Amp_R | RDB09596_A7Bdd.seq |
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>09596_09596_A7Bd_Amp_R_F05_17_ABI24.ab1 1 CTTCATTTCA TGACCGCTTT GGAAACAAGA CTGTATTTCC TGGAAATTAA TGTTTATTCA 61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG 121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC 181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA 241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG 301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA 361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG 421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT 481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC 541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC 601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG 721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT 781 AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG 841 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC 901 GCCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT 961 TTGGCAGTAC ATCAAGTGTA TCATATGCCA GGTACGCCCC CTATTGACGT CATGAACGTA 1021 AATGACCGCT GCATTATGCC AGTTACATGA CGTATGGACT TTACGTACTG GCAGTTACAT 1081 CTTACGTATA GATCAATCGC TAATTACCAA T // |
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Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl