RDB09596 - pPyCAG-Cre-IRESpuropA

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pPyCAG-Cre-IRESpuropA (#RDB09596)

Expression vector of Cre recombinase. Puromycin resistance.

Alternative name	pPyCAG-Cre-IP; stock no 547
Clone info.	Expression vector of Cre recombinase. Puromycin resistance.
Vector backbone	Plasmid
Gene/insert name	P1 phage Cre recombinase genomic DNA
Depositor\|Developer	Niwa, Hitoshi \|

Sequence	RDB09596z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB09596	pPyCAG-Cre-IRESpuropA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-Cre-IRESpuropA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09596).

Reference section:

Tashiro, F., Niwa, H., Miyazaki, J., Constructing adenoviral vectors by using the circular form of the adenoviral genome cloned in a cosmid and the Cre-loxP recombination system. Hum. Gene Ther. 10 (11): 1845-1852 (1999). PMID 10446924. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB09596_A7Bdp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB09596_A7Bde.seq
>09596_09596_A7Bd_pAxCA_F1_H02_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNTTNN NGCCTTCTTC TTTTTCCTAC NGCTCCTGGG CAACGTGCTG 61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTCGAG GGGCAGAGCC GATCCTGTAC 121 ACTTTACTTA AAACCATTAT CTGAGTGTGA AATGTCCAAT TTACTGACCG TACACCAAAA 181 TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT CGCAAGAACC TGATGGACAT 241 GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT CCGTTTGCCG 301 GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG TTTCCCGCAG AACCTGAAGA 361 TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG GCAGTAAAAA CTATCCAGCA 421 ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG CTGCCACGAC CAAGTGACAG 481 CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA AACGTTGATG CCGGTGAACG 541 TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC CAGGTTCGTT CACTCATGGA 601 AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT CTGGGGANTG CTTATAACAC 661 CCTGTTACGT ATAGCCGAAA TTGCCNGGNT CANGGTTAAA GANATCTCAC GTACTGACNG 721 TGGGAGAATG NNAATNCNTA TTGNCAGAAC NAAAACGCTG GTTANCACNG CNNNGTAGAN 781 NNNGNACTTA NNCTGGGGGT NACTAANCTG GTCNANCGAT GGANTTCCGT CTCTGGTGTA 841 NCTGANGANN CNNNNNANTN NNNNNTTTNN CNNGNNNANA AAAAANNNNN NNNNN //
Primer: IRES-R Sequence file: RDB09596_A7Bdf.seq
>09596_09596_A7Bd_IRES-R_F02_06_ABI08.ab1 1 NNNNNNNNNG NNNNNNNNCN NNNNNGTTAG GGGGGGGGGN NGGNNANGGG GGGGGGGCGG 61 NNTTCGATAT CAAGNNNNNN NNNNGGCCGA TCTCAGTGGT ATTTGTGAGC CAGGGCATTG 121 GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGAA TTCGCGTTAA 181 TGGCTAATCG CCATCTTCCA GCAGGCGCAC CATTGCCCCT GTTTCACTAT CCAGGTTACG 241 GATATAGTTC ATGACAATAT TTACATTGGT CCAGCCACCA GCTTGCATGA TCTCCGGTAT 301 TGAAACTCCA GCGCGGGCCA TATCTCGCGC GGCTCCGACA CGGGCACTGT GTCCAGACCA 361 GGCCAGGTAT CTCTGACCAG AGTCATCCTT AGCGCCGTAA ATCAATCGAT GAGTTGCTTC 421 AAAAATCCCT TCCAGGGCGC GAGTTGATAG CTGGCTGGTG GCAGATGGCG CGGCAACACC 481 ATTTTTTCTG ACCCGGCAAA ACAGGTAGTT ATTCGGATCA TCANCTACAC CAGAGACGGA 541 AATCCATCGC TCGACCNNNT TAGTTACCCC CAGGCTAANT GCCTTCTCTA CACCTGCGGT 601 GCTAACCANC GTTTTCGTTC TGCCAATATG GATTANNNNN NTCCCACCGT CAGTACGTGA 661 GATATCTTTA ACCCTGATCC TGGNNATTTC GGCTATACGT AACANGGTGT TATAAGCAAT 721 CCCCANNAAA TGCCAGATTA CNNATATCCT GGNAGCGATC GCTATTTTTC CATGANTGAA 781 CGAACCTGNT CNANATCAGT GCGNTNNGAN CNNCTANANC CTGTTTTGCA CNTNNACNGG 841 CNNNCAACGT TTTTCTTTTC NGATNCNNCN NCATNANCNA NNNGAAACNN NNNNNGNNTG 901 NNNNTNNNNN NNNGNNANCC CCGNNNNCNN CNANNANNCA NGTTTNANCT NGNCCNNANN 961 GNTNGCNNGN ANNANTTNTN NCTNNCNNGA NNNNNNNNNN //
Primer: IRES-F Sequence file: RDB09596_A7Bdg.seq
>09596_09596_A7Bd_IRES-F_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNTNNGT CGNNGNNNNN NANNGTCTAG GCCCCCCGAA CCACGGGGNN 61 GTGGTTTTCC TTTGNAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC 121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT 241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC 361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA 421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC 481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC 541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG 601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA 661 ANGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC 721 GCANCGCCCG ACCGAAAGGA GCGCACGANC CCATGCATCG ATGATCTAGA GCTCGCTGAT 781 CAGCCTCGAC TGTGCCTTCT ANTTGCCAGC CATCTGNTGT TTGCCCCTNC CCCGNGCCTT 841 CCTTGANCCT NGAAGGTGCC ACTCCCACTG NCCTTNNCTA ATAAAATGAN GAAATTGCAT 901 CGCNNTGNCT NNNNNNNTGT CATTCNANTC NNNNNNNNNN //
Primer: Amp_R Sequence file: RDB09596_A7Bdh.seq
>09596_09596_A7Bd_Amp_R_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNGCTTN NGAANNAAGA NTGTATTTCC TGGAANTTAA TGTTTATTCA 61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG 121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC 181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA 241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG 301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA 361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG 421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT 481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC 541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC 601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG 721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CNGGGNCATT 781 AGTTCATAGC CNATATATGG ANTTNCGCGT TACATAACTT ACNGTAAATG GCCCGCCNNG 841 CTGANCGCCC NACGACCCCC GCCNATTGNN GTCNATAATG ACNNANGTTC CCNNNNNNNN 901 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tashiro, F., Constructing adenoviral vectors by using the circular form of the adenoviral genome cloned in a cosmid and the Cre-loxP recombination system. Hum. Gene Ther. 10 (11): 1845-1852 (1999). PMID 10446924. [link to RRC of NBRP]