Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-Cre-IRESpuropA

Expression vector of Cre recombinase. Puromycin resistance.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tashiro, F., Hum. Gene Ther. 10 (11): 1845-1852, 1999)。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 [Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Sequence RDB09596z.seq
Electronic file	Map RDB09596a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB09596_A7Bdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Region: Cre 5' Sequence file: RDB09596_A7Bda.seq
>09596_09596_A7Bd_pAxCA_F1_H02_08_ABI08.ab1     1 CATTCCTTGC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG    61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTCGAG GGGCAGAGCC GATCCTGTAC   121 ACTTTACTTA AAACCATTAT CTGAGTGTGA AATGTCCAAT TTACTGACCG TACACCAAAA   181 TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT CGCAAGAACC TGATGGACAT   241 GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT CCGTTTGCCG   301 GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG TTTCCCGCAG AACCTGAAGA   361 TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG GCAGTAAAAA CTATCCAGCA   421 ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG CTGCCACGAC CAAGTGACAG   481 CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA AACGTTGATG CCGGTGAACG   541 TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC CAGGTTCGTT CACTCATGGA   601 AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT CTGGGGATTG CTTATAACAC   661 CCTGTTACGT ATAGCCGAAA TTGCCAGGAT CAGGGTTAAA GATATCTCAC GTACTGACGG   721 TGGGAGAATG TTAATCCATA TTGGCAGAAC GAAAACGCTG GTTAGCACCG CAGTGTAGAG   781 AAGGCACTTA GCCTGGGGGT AACTAAACTG GTCGAGCGAT GGATTTCCGT CTCTGGTGTA   841 GCTGATGATC CGAATAACTA CCTGTTTTGC CCGGGTCAGA AAAAATGGTG TTGCCGCGCC   901 ATCTGCCACC AGCCCAGCTA TCAACTCGCG CCCTGAAGGG ATTTTTGAAG CAACTCATCG   961 ATTGGATTAC GGCGCTAGAT GACCTCTGGT CAGAGAATAC CTGCTGATCT GGACACATTA  1021 CCCGTGTCGG ACCGCCGCGA GAATATGGCC CGGCCTGGAG TTCTAATCGA ATCATGCAGC 1081 TGGGTGCCTG GAACCAATGT GTA //
Primer: IRES-R Region: IRES2, Cre 3' Sequence file: RDB09596_A7Bdb.seq
>09596_09596_A7Bd_IRES-R_F02_06_ABI08.ab1     1 GGCTCGCGGG CTTCGGCCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGCGGA    61 ATTCGATATC AAGCTTATCG AGCGGCCGAT CTCAGTGGTA TTTGTGAGCC AGGGCATTGG   121 CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT TCGCGTTAAT   181 GGCTAATCGC CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG   241 ATATAGTTCA TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT   301 GAAACTCCAG CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG   361 GCCAGGTATC TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA   421 AAAATCCCTT CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA   481 TTTTTTCTGA CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA   541 ATCCATCGCT CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG   601 CTAACCAGCG TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG   661 ATATCTTTAA CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAAGCAAT   721 CCCCAGAAAA TGCCAGATTA CGGTATATCC TGGCAGCGAT CGCTATTTTT CCATGAGTGA   781 ACGAACCTGG GTCGAAATCA GTGCGTTTCG AACGGCTAGA GCCTGTTTTG CACGTTCACC   841 GGCAATCAAC GTTTTTCTTT TCGGATCCCG CCGCATAACC CAGGTGAAAC AGCAATTTGC   901 CTGTCACTTT GGTTCGTGGC CAGCCCCGGA ACCGGACGAT GAAAGCCATG TTTTAGCCTG   961 GGCCCCAAAT GTTTGCCTGG GAATAGTTTT TTACTGGCCA GGACCGCCGC GCCTTGAAGA  1021 ATTATAGGAG AGAATAATTC GCGAAACCAT TCTTTCCAGG GTTCGTGCCG GGGAAAACCA  1081 ATATCCCGGG TTTATTTCCA ACTTTGCCAC CCAATGCCCG GTCCCAACGC GAAACTCGCG 1141 GGGGCACAGA CGACG //
Primer: IRES-F Region: IRES2, PuroR, BGH p(A) Sequence file: RDB09596_A7Bdc.seq
>09596_09596_A7Bd_IRES-F_E02_05_ABI08.ab1     1 GCATGCTTCA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC   121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC   181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT   241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT   301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC   361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA   421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC   481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC   541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG   601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA   661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC   721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT   781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT   841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT   901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGTTGG GTTGGGGCAG GACAGCCAAG   961 GGGGGGAGGG ATTTGGGAAG AACAATTAGG CAGGCATGGC TTGGGAATGC CGTTGGGCCT 1021 ATATTGGCCT TCTGTAGACC GGGAAAGGAA ACTGCTGCTG TC //
Primer: Amp_R Region: Polyoma Ori, SV40 p(A), HCMVIEE Sequence file: RDB09596_A7Bdd.seq
>09596_09596_A7Bd_Amp_R_F05_17_ABI24.ab1     1 CTTCATTTCA TGACCGCTTT GGAAACAAGA CTGTATTTCC TGGAAATTAA TGTTTATTCA    61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG   121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC   181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA   241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG   301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA   361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG   421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT   481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC   541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC   601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC   661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG   721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT   781 AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG   841 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC   901 GCCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT   961 TTGGCAGTAC ATCAAGTGTA TCATATGCCA GGTACGCCCC CTATTGACGT CATGAACGTA  1021 AATGACCGCT GCATTATGCC AGTTACATGA CGTATGGACT TTACGTACTG GCAGTTACAT 1081 CTTACGTATA GATCAATCGC TAATTACCAA T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl