Resource data sheet
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pCAGGS lacZ

Expression clone of lacZ.

Catalog number RDB09309
Resource name pCAGGS lacZ
Alternative name stock no 240
Clone info. Expression clone of lacZ under the control of CAG promoter.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name E. coli lacZ genomic DNA
Depositor Niwa, Hitoshi |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
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RDB09309 pCAGGS lacZ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB09309_A9Fcp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 RDB09309_A9Fca.seq
>09309_09309_A9Fc_2_CAG_R1_C05_08_ABI24.ab1
    1 TGAAACGCGG CCGCACCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA
   61 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA
  121 GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT
  181 CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT
  241 CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA
  301 AGAATTCAAG CTTGGGATCT CTATAATCTC GCGCAACCTA TTTTCCCCTC GAACACTTTT
  361 TAAGCCGTAG ATAAACAGGC TGGGACACTT CACATGAGCG AAAAATACAT CGTCACCTGG
  421 GACATGTTGC AGATCCATGC ACGTAAACTC GCAAGCCGAC TGATGCCTTC TGAACAATGG
  481 AAAGGCATTA TTGCCGTAAG CCGTGGCGGT CTGGTACCGG TGGGTGAAGA CCAGAAACAG
  541 CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG CGAGATCGAT
  601 CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT
  661 GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT
  721 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA
  781 GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC
  841 TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT
  901 ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT
  961 AATGTTGATG AAAGCTGGCT ACAGAAGGCC AGACGCGAAT TATTTTTTGA TGGCGTTAAC
 1021 TCGGCGTTTC ATCTGTGGTG CAACGGGCGC CTGGGTCGGT TACGGCCAGG ACAGGTCGTT
 1081 TTGTCGTCCT GGAATTGGAA CATGAAGCGC CAGTTTTTAC GCCCGCCCCC GGGAAGAAAA
1141 AAAA
//
Primer: pCAGGS_R1 RDB09309_A9Fcb.seq
>09309_09309_A9Fc_2pCAGGS_R1_D05_11_ABI24.ab1
    1 AAAAATTCCA ACACACTATT GCAATGAAAA TAAATTTCCT TTATTAGCCA GAAGTCAGAT
   61 GCTCAAGGGG CTTCATGATG TCCCCATAAT TTTTGGCAGA GGGAAAAAGA TCTCAGTGGT
  121 ATTTGTGAGC CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT
  181 GAGGAGTGAA TTCCTCGACG GATCCCCCCT GCCCGGTTAT TATTATTTTT GACACCAGAC
  241 CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATTCC GCCGATACTG ACGGGCTCCA
  301 GGAGTCGTCG CCACCAATCC CCATATGGAA ACCGTCGATA TTCAGCCATG TGCCTTCTTC
  361 CGCGTGCAGC AGATGGCGAT GGCTGGTTTC CATCAGTTGC TGTTGACTGT AGCGGCTGAT
  421 GTTGAACTGG AAGTCGCCGC GCCACTGGTG TGGGCCATAA TTCAATTCGC GCGTCCCGCA
  481 GCGCAGACCG TTTTCGCTCG GGAAGACGTA CGGGGTATAC ATGTCTGACA ATGGCAGATC
  541 CCAGCGGTCA AAACAGGCGG CAGTAAGGCG GTCGGGATAG TTTTCTTGCG GCCCTAATCC
  601 GAGCCAGTTT ACCCGCTCTG CTACCTGCGC CAGCTGGCAG TTCAGGCCAA TCCGCGCCGG
  661 ATGCGGTGTA TCGCTCGCCA CTTCAACATC AACGGTAATC GCCATTTGAC CACTACCATC
  721 AATCCGGTAG GTTTTCCGGC TGATAAATAA GGTTTTCCCC TGATGCTGCC ACGCGTGAGC
  781 GGTCGTAATC AGCACCGCAT CAGCAAGTGT ATCTGCCGTG CACTGCAACA ACGCTGCTTC
  841 GGCCTGGTAA TGGCCCGCCG CCTTCCAGCG TTCGACCCAG GCGTTAGGGT CAATGCGGGT
  901 CGCTTCACTT ACGCCAATGT CGTTATCCAG CGGTGCACGG GTGAACTGAT CGCGCAGCGG
  961 CGTCAGCAGT TGTTTTTTTA TCGCCCAATC CACATCTGTG AAAGAAAGCC CTGACTGGGC
 1021 GGTTAAATTT GCCCAACGCT TATTACCCAG CTCGATGCAA AAATCCCATT TCGCTTGTTG
 1081 TTCAGATGCG GGATTGGCCA TGGACGCGCG GGGCAGCGTT CACACTGAGG ATTTTTCCCG
1141 CCAAGAACGG TCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.07.02

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