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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-BstXI-IREShygropA

Expression vector of CAG promoter, IRES-hygromycin resistance.

Catalog number RDB09233
Resource name pPyCAG-BstXI-IREShygropA
Alternative name pPyCAG-BstXI-IH; pPyCAGBstXIIREShygropA(+ori); stock no 160
Clone info. Expression vector of CAG promoter and b-globin poly(A) (with SV40 ori) with IRES-hygromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG-BstXI-IREShygropA (Plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells)
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database

            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB09233p.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09233 pPyCAG-BstXI-IREShygropA DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pPyCAG-BstXI-IREShygropA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09233).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09233_A7Ilp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCAG-F_V2 (Pr0330)
            Region: CAG pro,BstXI-stuffer
            Sequence file: RDB09233_A7Ile.seq check
            >09233_09233_1_A7Il_pCAG-F_V2_A07_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNTGNGCGG NNCCGANTCT GGGNNGCGCC GCCGCACCCC
               61 CTCTAGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG
              121 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG
              181 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC
              241 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT
              301 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGATCCA
              361 TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC GGGCATTCCC GATCAGAAAT
              421 GAGCGCCAGT CGTCGTCGGC TCTCGGCACC GAATGCGTAT GATTCTCCGC CAGCATGGCT
              481 TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC
              541 TGAACCCCCA ACCGTTCCGC CAGTTTGCGT GTCGTCAGAC CGTCTACGCC GACCTCGTTC
              601 AACAGGTCCA GGGCGGCACG GATCACTGTA TTCGGCTGCA ACTTTGTCAT GCTTGACACT
              661 TTATCACTGA TAAACATAAT ATGTCCACCA ACTTATCAGT GATAAAGAAT CCGCGCCAGC
              721 ACAATGGATC TCGAGGAATT CACTCCTCAG GTGCAGGCTG CCTATCAGAA NGTGGTGGCT
              781 GGTGTGGCCA ACTGGCTCAC AAATACCACT GAGATCGACG GTATCGATAA GCTTGATATC
              841 GAANTCCNNC CCCCCCCCCC TCTCCCNCNC CCCCCCCCNN AACGTTACNG GCCGAANNCN
              901 NCTNGNAANA NGNCGGNGTG CGTTNGNNNA TATGTNNTTT NNCACCATAT NNNCGTCTTT
            961 NNNNNANGTG ANGGNNNGNA AANNNNNNNN NN
            //
            Primer: IRES-F (Pr0036)
            Region: IRES,HygR
            Sequence file: RDB09233_A7Ilf.seq check
            >09233_09233_1_A7Il_IRES-F_B07_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTNN GTCGAGGTTN NNAAACGTCT AGGCCCCCCG AACCNCGGGG
               61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAA AAAGCCTGAA
              121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG
              181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA
              241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTCATCGG
              301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG
              361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA
              421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC
              481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT
              541 ACGTGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG
              601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC
              661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG
              721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGNNTCN
              781 NAATACGANG TCGCCNACAT CTTCTTCTGG ANGCCGNGGT TGGCTTGTAT GGANCAGCAG
              841 ACGCGCTACT TCNANCGGAN GCNTNCGGAN CTTGNNNNAT CGCCGCGGNN NCNGGCGTAT
              901 ATGCTNNNNA TTNNNCTNGN CCNACTCTAT CNNANCTTNG NTNNACGGNN NTTTCNATGN
              961 NGCNGCTTNN NNGCNNGNNC NANGNNNNGC NNNNNNNNNA TNCNGNNNNC NGNNNNNNNN
            1021 NGNNNNNNNN NN
            //
            Primer: IRES_R3 (Pr0573)
            Region: BstXI-stuffer
            Sequence file: RDB09233_A7Ilg.seq check
            >09233_09233_B3IjF_1_IRES_R3_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNGNN NGGNNNNGGG GGGGGGGCGG NNNTCGNTAT CAAGCTTATC
               61 GATACCGTCG ATCTCNNTGG TATTTGTGAG CCAGTTGGCC ACACCAGCCA CCACCTTCTG
              121 ATAGGCAGCC TGCACCTGAG GAGTGAATTC CTCGAGATCC ATTGTGCTGG CGCGGATTCT
              181 TTATCACTGA TAAGTTGGTG GACATATTAT GTTTATCAGT GATAAAGTGT CAAGCATGAC
              241 AAAGTTGCAG CCGAATACAG TGATCCGTGC CGCCCTGGAC CTGTTGAACG AGGTCGGCGT
              301 AGACGGTCTG ACGACACGCA AACTGGCGGA ACGGTTGGGG GTTCAGCAGC CGGCGCTTTA
              361 CTGGCACTTC AGGAACAAGC GGGCGCTGCT CGACGCACTG GCCGAAGCCA TGCTGGCGGA
              421 GAATCATACG CATTCGGTGC CGAGAGCCGA CGACGACTGG CGCTCATTTC TGATCGGGAA
              481 TGCCCGCAGC TTCAGGCAGG CGCTGCTCGC CTACCGCCAG CACAATGGAT CTCGAGGAAT
              541 TCTTTGCCAA AATGATGAGA CAGCACAATA ACCAGCACGT TGCCCAGGAG CTGTAGGAAA
              601 AAGAAGAAGG CATGAACATG GTTANNNNAG GCTCTAGAGC CGCCGGTCAC ACGCCAGAAG
              661 CCGAACCCCG CCCTGCCCCG TCCCCCCCGA AGGCAGCCGT CCCCCCGCGG ANAGCCCCGA
              721 NGCTGGANAG GGAGAANGGG ANGGCGGCGC GGCNNNNCAC GANNGNCCNT CCCCGCCNAT
              781 TTNCNTTCCT GCNNGCGCNG CACCGCTTCN CCCCGNGCCC GCTANANGGG GTGCGGCNGN
              841 NNCCTNCCCN NANTTNCNGC NNCNNNNNAN NNTNNGGNNN NANNNGNANN NCCCNTGCGC
            901 CCNTCTCNCN NGANNNNNNN NAAAANGGNA ANNGGCTGCN GNNNNNNNNN N
            //
            Primer: CMV_en_R (Pr0173)
            Region: CAG pro,polyoma ori
            Sequence file: RDB09233_A7Ilh.seq check
            >09233_09233_1_A7Il_CMV_en_R_D07_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT NNNNAACTAG TCAATAATCA ATGTCGACGA TGATATCAGA
               61 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
              121 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
              181 CAATAAACAA GTTAATTCGA TTCAGCTTCA GAAGATGGCG GAGGGCCTCC AACACAGTAA
              241 TTTTCCTCCC GACTCTTAAA ATAGAAAATG TCAAGTCAGT TAAGCAGGAA GTGACTAACT
              301 GACCGCAGCT GGCCGTGCGA CATCCTCTTT TAATTAGTTG CTAGGCAACT GCCCTCCAGA
              361 GGGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTCAG
              421 TGTGGTTTTG CAAGAGGAAG CAAAAAGCCT CTCCACCCAG GCCTGGAATG TTTCCACCCA
              481 ATCATTACTA TGACAACAGC TGTTTTTTTT AGTATTAAGC AGAGGCCGGG GGCCCCTGGC
              541 CTCCGCTTAC TCTGGAGAAA AAGAAGAGAG GCTTCCAGAG GCAACTTGTC AAAACAGGAC
              601 TGGCGCCTTG GAGGCGCTGT GGGGCCACCC AAATTGATAT AATTAAGCCC CAACCGCCTC
              661 TTCCCGCCTC ATTTCAGCCT CACCACTATG TGAATATAGC TGAATACACA GTTTATTGAA
              721 TAAACATTAA TTTCCAGGAA ATACAGTCTT TGTTTTTCCA AAGCGGTCAT TGAAAAAGGA
              781 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
              841 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGANGCTGAA GANCAGTTGG
              901 GNGNANGANN GGGTTNNNTC GAANNGGATC TCNNCAGCNG TAAGATCCNT NANANTTTTC
            961 GCCCCGAANA ANGTTTTNNN NNGATGANCN NNTTTNANNN NNNGNTANNN NNNNNNN
            //
            Primer: HygR-C_F (Pr0501)
            Region: HygR
            Sequence file: RDB09233_A7Ili.seq check
            >09233_09233_A7Il_HygR-C_F_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT TCTTCTGGAG GCNNNNNNTG
               61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC NAGCGGAGGC ATCCGGAGCT TGCAGGATCG
              121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT
              181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC
              241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT
              301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA
              361 AAGGAATAGT CGAGAAATTG ATGATCTATT AAGCAATAAN GACGTCCACT AAAATGGAAG
              421 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA
              481 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTANAT NAATGNNNGN NCNNNNNNGN
              541 NNGNTCNTNN NNNNTGNNNN NNGANANTGN TNCANNNNNN NANNNNANNN NTNNNNNANN
            601 CNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.09.29

            GNP_filter3_RDBDEP_html_230929.pl