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pPyCAG-BstXI-IREShygropA

Expression vector of CAG promoter, IRES-hygromycin resistance.

Catalog number RDB09233
Resource name pPyCAG-BstXI-IREShygropA
Alternative name pPyCAG-BstXI-IH; pPyCAGBstXIIREShygropA(+ori); stock no 160
Clone info. Expression vector of CAG promoter and b-globin poly(A) (with SV40 ori) with IRES-hygromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG-BstXI-IREShygropA (Plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells)
Depositor|Developer Niwa, Hitoshi |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB09233 pPyCAG-BstXI-IREShygropA DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB09233p.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09233_A7Ilp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCAG-F_V2
            Sequence file: RDB09233_A7Ila.seq check
            >09233_09233_1_A7Il_pCAG-F_V2_A07_01_ABI24.ab1
                1 TCGAGAGCGG CGACGTGTCA ATCTGTGCGG AGCCGAATCT GGGAGGCGCC GCCGCACCCC
               61 CTCTAGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG
              121 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG
              181 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC
              241 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT
              301 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGATCCA
              361 TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC GGGCATTCCC GATCAGAAAT
              421 GAGCGCCAGT CGTCGTCGGC TCTCGGCACC GAATGCGTAT GATTCTCCGC CAGCATGGCT
              481 TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC
              541 TGAACCCCCA ACCGTTCCGC CAGTTTGCGT GTCGTCAGAC CGTCTACGCC GACCTCGTTC
              601 AACAGGTCCA GGGCGGCACG GATCACTGTA TTCGGCTGCA ACTTTGTCAT GCTTGACACT
              661 TTATCACTGA TAAACATAAT ATGTCCACCA ACTTATCAGT GATAAAGAAT CCGCGCCAGC
              721 ACAATGGATC TCGAGGAATT CACTCCTCAG GTGCAGGCTG CCTATCAGAA GGTGGTGGCT
              781 GGTGTGGCCA ACTGGCTCAC AAATACCACT GAGATCGACG GTATCGATAA GCTTGATATC
              841 GAATTCCGCC CCCCCCCCCC TCTCCCTCGC CCCCCCCCCT AACGTTACTG GCCGAAGCCC
              901 GCTTGGAATA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT
              961 TGGCAATGTG AGGGCCGGGA AACCTGGCCC CTGTCTTCTT TGACGAGCAT TCCCTAGGGG
             1021 GGTCTTTTCC CCTCTCGCCA AAGGGAATGC AAGGTCTGTT TGAATGTCCG TGAAGAAGCC
             1081 AGTTCCTTCT GGGAAGCCTC CTTGAGACAA CAAACGGTCT GGTAGCGACT TGCCAGGCAG
            1141 CCGAGCTCTC CACCTGGGCG ATACAGGTGT CCCTCTCTGC CG
            //
            Primer: IRES-F
            Sequence file: RDB09233_A7Ilb.seq check
            >09233_09233_1_A7Il_IRES-F_B07_04_ABI24.ab1
                1 GGCAGGCGTT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
               61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAA AAAGCCTGAA
              121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG
              181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA
              241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTCATCGG
              301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG
              361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA
              421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC
              481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT
              541 ACGTGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG
              601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC
              661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG
              721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC
              781 CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG
              841 ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT
              901 ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTTGACGGCA ATTTCGATGA
              961 TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC CGGGACTGTC
             1021 GGGCGTACAC AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC
             1081 TCGCGATAGT GAAACCGACG CCCCCAGCAC TCGTCGAAGG CCAAGATTAG TTCGAAGAAA
            1141 TTGATGGAAT CCTATTTAAG GCCAAAATTA
            //
            Primer: HygR-C_F
            Sequence file: RDB09233_A7Ilc.seq check
            >09233_09233_A7Il_HygR-C_F_E01_05_ABI08.ab1
                1 ATGATTCGGG GGGATTTCCA TTCGAGGTTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG
               61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG
              121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT
              181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC
              241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT
              301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA
              361 AAGGAATAGT CGAGAAATTG ATGATCTATT AAGCAATAAA GACGTCCACT AAAATGGAAG
              421 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA
              481 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA
              541 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG
              601 CAAAAACCAA ATTAAGGGCC AGCTCATTCC TCCACTCACG ATCTATAGAT CCACTAGCTT
            661 GGCGTAATCT GGGTCATAGC TGG
            //
            Primer: CMV_en_R
            Sequence file: RDB09233_A7Ild.seq check
            >09233_09233_1_A7Il_CMV_en_R_D07_10_ABI24.ab1
                1 GTGGGGTAAT GGATTACTAT TAATAACTAG TCAATAATCA ATGTCGACGA TGATATCAGA
               61 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
              121 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
              181 CAATAAACAA GTTAATTCGA TTCAGCTTCA GAAGATGGCG GAGGGCCTCC AACACAGTAA
              241 TTTTCCTCCC GACTCTTAAA ATAGAAAATG TCAAGTCAGT TAAGCAGGAA GTGACTAACT
              301 GACCGCAGCT GGCCGTGCGA CATCCTCTTT TAATTAGTTG CTAGGCAACT GCCCTCCAGA
              361 GGGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTCAG
              421 TGTGGTTTTG CAAGAGGAAG CAAAAAGCCT CTCCACCCAG GCCTGGAATG TTTCCACCCA
              481 ATCATTACTA TGACAACAGC TGTTTTTTTT AGTATTAAGC AGAGGCCGGG GGCCCCTGGC
              541 CTCCGCTTAC TCTGGAGAAA AAGAAGAGAG GCTTCCAGAG GCAACTTGTC AAAACAGGAC
              601 TGGCGCCTTG GAGGCGCTGT GGGGCCACCC AAATTGATAT AATTAAGCCC CAACCGCCTC
              661 TTCCCGCCTC ATTTCAGCCT CACCACTATG TGAATATAGC TGAATACACA GTTTATTGAA
              721 TAAACATTAA TTTCCAGGAA ATACAGTCTT TGTTTTTCCA AAGCGGTCAT TGAAAAAGGA
              781 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
              841 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
              901 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
              961 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
             1021 TATCCCGTAT TGACGCCCGG GCAAGAGCAA CTCGGGTCGC CGCATACACT ATTCTCAGAA
             1081 TGACTGGTGA GTACTCACCA GTCACAGAAG CATCTACGGA ATGGCCATGA CAGTAGAGAA
            1141 TTATGCCAGG TGCCTGTGCA T
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl