Resource data sheet
Prev.

pPyCAG-BstXI-IREShygropA

Expression vector of CAG promoter, IRES-hygromycin resistance.

Catalog number RDB09233
Resource name pPyCAG-BstXI-IREShygropA
Alternative name pPyCAG-BstXI-IH; pPyCAGBstXIIREShygropA(+ori); stock no 160
Clone info. Expression vector of CAG promoter and b-globin poly(A) (with SV40 ori) with IRES-hygromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG-BstXI-IREShygropA (Plasmid)
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cells)
Depositor Niwa, Hitoshi |

Distribution information

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RDB09233 pPyCAG-BstXI-IREShygropA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB09233p.pdf from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB09233_A7Ilp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 RDB09233_A7Ila.seq
>09233_09233_1_A7Il_pCAG-F_V2_A07_01_ABI24.ab1
    1 TCGAGAGCGG CGACGTGTCA ATCTGTGCGG AGCCGAATCT GGGAGGCGCC GCCGCACCCC
   61 CTCTAGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG
  121 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG
  181 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC
  241 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT
  301 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGATCCA
  361 TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC GGGCATTCCC GATCAGAAAT
  421 GAGCGCCAGT CGTCGTCGGC TCTCGGCACC GAATGCGTAT GATTCTCCGC CAGCATGGCT
  481 TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC
  541 TGAACCCCCA ACCGTTCCGC CAGTTTGCGT GTCGTCAGAC CGTCTACGCC GACCTCGTTC
  601 AACAGGTCCA GGGCGGCACG GATCACTGTA TTCGGCTGCA ACTTTGTCAT GCTTGACACT
  661 TTATCACTGA TAAACATAAT ATGTCCACCA ACTTATCAGT GATAAAGAAT CCGCGCCAGC
  721 ACAATGGATC TCGAGGAATT CACTCCTCAG GTGCAGGCTG CCTATCAGAA GGTGGTGGCT
  781 GGTGTGGCCA ACTGGCTCAC AAATACCACT GAGATCGACG GTATCGATAA GCTTGATATC
  841 GAATTCCGCC CCCCCCCCCC TCTCCCTCGC CCCCCCCCCT AACGTTACTG GCCGAAGCCC
  901 GCTTGGAATA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT
  961 TGGCAATGTG AGGGCCGGGA AACCTGGCCC CTGTCTTCTT TGACGAGCAT TCCCTAGGGG
 1021 GGTCTTTTCC CCTCTCGCCA AAGGGAATGC AAGGTCTGTT TGAATGTCCG TGAAGAAGCC
 1081 AGTTCCTTCT GGGAAGCCTC CTTGAGACAA CAAACGGTCT GGTAGCGACT TGCCAGGCAG
1141 CCGAGCTCTC CACCTGGGCG ATACAGGTGT CCCTCTCTGC CG
//
Primer: IRES-F RDB09233_A7Ilb.seq
>09233_09233_1_A7Il_IRES-F_B07_04_ABI24.ab1
    1 GGCAGGCGTT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAA AAAGCCTGAA
  121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG
  181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA
  241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTCATCGG
  301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG
  361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA
  421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC
  481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT
  541 ACGTGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG
  601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC
  661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG
  721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC
  781 CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG
  841 ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT
  901 ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTTGACGGCA ATTTCGATGA
  961 TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC CGGGACTGTC
 1021 GGGCGTACAC AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC
 1081 TCGCGATAGT GAAACCGACG CCCCCAGCAC TCGTCGAAGG CCAAGATTAG TTCGAAGAAA
1141 TTGATGGAAT CCTATTTAAG GCCAAAATTA
//
Primer: HygR-C_F RDB09233_A7Ilc.seq
>09233_09233_A7Il_HygR-C_F_E01_05_ABI08.ab1
    1 ATGATTCGGG GGGATTTCCA TTCGAGGTTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG
   61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG
  121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT
  181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC
  241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT
  301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA
  361 AAGGAATAGT CGAGAAATTG ATGATCTATT AAGCAATAAA GACGTCCACT AAAATGGAAG
  421 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA
  481 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA
  541 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG
  601 CAAAAACCAA ATTAAGGGCC AGCTCATTCC TCCACTCACG ATCTATAGAT CCACTAGCTT
661 GGCGTAATCT GGGTCATAGC TGG
//
Primer: CMV_en_R RDB09233_A7Ild.seq
>09233_09233_1_A7Il_CMV_en_R_D07_10_ABI24.ab1
    1 GTGGGGTAAT GGATTACTAT TAATAACTAG TCAATAATCA ATGTCGACGA TGATATCAGA
   61 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
  121 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
  181 CAATAAACAA GTTAATTCGA TTCAGCTTCA GAAGATGGCG GAGGGCCTCC AACACAGTAA
  241 TTTTCCTCCC GACTCTTAAA ATAGAAAATG TCAAGTCAGT TAAGCAGGAA GTGACTAACT
  301 GACCGCAGCT GGCCGTGCGA CATCCTCTTT TAATTAGTTG CTAGGCAACT GCCCTCCAGA
  361 GGGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTCAG
  421 TGTGGTTTTG CAAGAGGAAG CAAAAAGCCT CTCCACCCAG GCCTGGAATG TTTCCACCCA
  481 ATCATTACTA TGACAACAGC TGTTTTTTTT AGTATTAAGC AGAGGCCGGG GGCCCCTGGC
  541 CTCCGCTTAC TCTGGAGAAA AAGAAGAGAG GCTTCCAGAG GCAACTTGTC AAAACAGGAC
  601 TGGCGCCTTG GAGGCGCTGT GGGGCCACCC AAATTGATAT AATTAAGCCC CAACCGCCTC
  661 TTCCCGCCTC ATTTCAGCCT CACCACTATG TGAATATAGC TGAATACACA GTTTATTGAA
  721 TAAACATTAA TTTCCAGGAA ATACAGTCTT TGTTTTTCCA AAGCGGTCAT TGAAAAAGGA
  781 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
  841 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
  901 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
  961 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
 1021 TATCCCGTAT TGACGCCCGG GCAAGAGCAA CTCGGGTCGC CGCATACACT ATTCTCAGAA
 1081 TGACTGGTGA GTACTCACCA GTCACAGAAG CATCTACGGA ATGGCCATGA CAGTAGAGAA
1141 TTATGCCAGG TGCCTGTGCA T
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.11

GNP_filter3_RDBDEP_html_181012.pl