Sequencing and PCR primers for primer information.
Expression vector of CAG promoter, IRES-hygromycin resistance.
| Catalog number | RDB09233 |
|---|---|
| Resource name | pPyCAG-BstXI-IREShygropA |
| Alternative name | pPyCAG-BstXI-IH; pPyCAGBstXIIREShygropA(+ori); stock no 160 |
| Clone info. | Expression vector of CAG promoter and b-globin poly(A) (with SV40 ori) with IRES-hygromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20. |
| Vector backbone | pPyCAG-BstXI-IREShygropA (Plasmid) |
| Selectable markers | Amp^r (E. coli), Hygro^r (mammalian cells) |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB09233 | pPyCAG-BstXI-IREShygropA | DNA solution |
Electronic file
| Electronic file | PDF RDB09233p.pdf from Depositor |
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Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB09233_A7Ilp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pCAG-F_V2 | RDB09233_A7Ila.seq |
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>09233_09233_1_A7Il_pCAG-F_V2_A07_01_ABI24.ab1 1 TCGAGAGCGG CGACGTGTCA ATCTGTGCGG AGCCGAATCT GGGAGGCGCC GCCGCACCCC 61 CTCTAGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG 121 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG 181 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC 241 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT 301 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGATCCA 361 TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC GGGCATTCCC GATCAGAAAT 421 GAGCGCCAGT CGTCGTCGGC TCTCGGCACC GAATGCGTAT GATTCTCCGC CAGCATGGCT 481 TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC 541 TGAACCCCCA ACCGTTCCGC CAGTTTGCGT GTCGTCAGAC CGTCTACGCC GACCTCGTTC 601 AACAGGTCCA GGGCGGCACG GATCACTGTA TTCGGCTGCA ACTTTGTCAT GCTTGACACT 661 TTATCACTGA TAAACATAAT ATGTCCACCA ACTTATCAGT GATAAAGAAT CCGCGCCAGC 721 ACAATGGATC TCGAGGAATT CACTCCTCAG GTGCAGGCTG CCTATCAGAA GGTGGTGGCT 781 GGTGTGGCCA ACTGGCTCAC AAATACCACT GAGATCGACG GTATCGATAA GCTTGATATC 841 GAATTCCGCC CCCCCCCCCC TCTCCCTCGC CCCCCCCCCT AACGTTACTG GCCGAAGCCC 901 GCTTGGAATA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT 961 TGGCAATGTG AGGGCCGGGA AACCTGGCCC CTGTCTTCTT TGACGAGCAT TCCCTAGGGG 1021 GGTCTTTTCC CCTCTCGCCA AAGGGAATGC AAGGTCTGTT TGAATGTCCG TGAAGAAGCC 1081 AGTTCCTTCT GGGAAGCCTC CTTGAGACAA CAAACGGTCT GGTAGCGACT TGCCAGGCAG 1141 CCGAGCTCTC CACCTGGGCG ATACAGGTGT CCCTCTCTGC CG // |
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| Primer: IRES-F | RDB09233_A7Ilb.seq |
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>09233_09233_1_A7Il_IRES-F_B07_04_ABI24.ab1 1 GGCAGGCGTT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG 61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAA AAAGCCTGAA 121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG 181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA 241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTCATCGG 301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG 361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA 421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC 481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT 541 ACGTGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG 601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC 661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG 721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC 781 CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG 841 ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT 901 ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTTGACGGCA ATTTCGATGA 961 TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGGAGCC CGGGACTGTC 1021 GGGCGTACAC AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC 1081 TCGCGATAGT GAAACCGACG CCCCCAGCAC TCGTCGAAGG CCAAGATTAG TTCGAAGAAA 1141 TTGATGGAAT CCTATTTAAG GCCAAAATTA // |
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| Primer: HygR-C_F | RDB09233_A7Ilc.seq |
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>09233_09233_A7Il_HygR-C_F_E01_05_ABI08.ab1 1 ATGATTCGGG GGGATTTCCA TTCGAGGTTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG 61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG 121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT 181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC 241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT 301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA 361 AAGGAATAGT CGAGAAATTG ATGATCTATT AAGCAATAAA GACGTCCACT AAAATGGAAG 421 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA 481 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA 541 AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG 601 CAAAAACCAA ATTAAGGGCC AGCTCATTCC TCCACTCACG ATCTATAGAT CCACTAGCTT 661 GGCGTAATCT GGGTCATAGC TGG // |
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| Primer: CMV_en_R | RDB09233_A7Ild.seq |
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>09233_09233_1_A7Il_CMV_en_R_D07_10_ABI24.ab1 1 GTGGGGTAAT GGATTACTAT TAATAACTAG TCAATAATCA ATGTCGACGA TGATATCAGA 61 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 121 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 181 CAATAAACAA GTTAATTCGA TTCAGCTTCA GAAGATGGCG GAGGGCCTCC AACACAGTAA 241 TTTTCCTCCC GACTCTTAAA ATAGAAAATG TCAAGTCAGT TAAGCAGGAA GTGACTAACT 301 GACCGCAGCT GGCCGTGCGA CATCCTCTTT TAATTAGTTG CTAGGCAACT GCCCTCCAGA 361 GGGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTCAG 421 TGTGGTTTTG CAAGAGGAAG CAAAAAGCCT CTCCACCCAG GCCTGGAATG TTTCCACCCA 481 ATCATTACTA TGACAACAGC TGTTTTTTTT AGTATTAAGC AGAGGCCGGG GGCCCCTGGC 541 CTCCGCTTAC TCTGGAGAAA AAGAAGAGAG GCTTCCAGAG GCAACTTGTC AAAACAGGAC 601 TGGCGCCTTG GAGGCGCTGT GGGGCCACCC AAATTGATAT AATTAAGCCC CAACCGCCTC 661 TTCCCGCCTC ATTTCAGCCT CACCACTATG TGAATATAGC TGAATACACA GTTTATTGAA 721 TAAACATTAA TTTCCAGGAA ATACAGTCTT TGTTTTTCCA AAGCGGTCAT TGAAAAAGGA 781 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 841 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 901 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 961 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT 1021 TATCCCGTAT TGACGCCCGG GCAAGAGCAA CTCGGGTCGC CGCATACACT ATTCTCAGAA 1081 TGACTGGTGA GTACTCACCA GTCACAGAAG CATCTACGGA ATGGCCATGA CAGTAGAGAA 1141 TTATGCCAGG TGCCTGTGCA T // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl