Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-BstXI-IREShygropA

Expression vector of CAG promoter, IRES-hygromycin resistance.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB09233_A7Ilp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330) Region: CAG pro,BstXI-stuffer Sequence file: RDB09233_A7Ile.seq
>09233_09233_1_A7Il_pCAG-F_V2_A07_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTGNGCGG NNCCGANTCT GGGNNGCGCC GCCGCACCCC    61 CTCTAGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG   121 CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG   181 GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC   241 CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT   301 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGATCCA   361 TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC CTGAAGCTGC GGGCATTCCC GATCAGAAAT   421 GAGCGCCAGT CGTCGTCGGC TCTCGGCACC GAATGCGTAT GATTCTCCGC CAGCATGGCT   481 TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC   541 TGAACCCCCA ACCGTTCCGC CAGTTTGCGT GTCGTCAGAC CGTCTACGCC GACCTCGTTC   601 AACAGGTCCA GGGCGGCACG GATCACTGTA TTCGGCTGCA ACTTTGTCAT GCTTGACACT   661 TTATCACTGA TAAACATAAT ATGTCCACCA ACTTATCAGT GATAAAGAAT CCGCGCCAGC   721 ACAATGGATC TCGAGGAATT CACTCCTCAG GTGCAGGCTG CCTATCAGAA NGTGGTGGCT   781 GGTGTGGCCA ACTGGCTCAC AAATACCACT GAGATCGACG GTATCGATAA GCTTGATATC   841 GAANTCCNNC CCCCCCCCCC TCTCCCNCNC CCCCCCCCNN AACGTTACNG GCCGAANNCN   901 NCTNGNAANA NGNCGGNGTG CGTTNGNNNA TATGTNNTTT NNCACCATAT NNNCGTCTTT 961 NNNNNANGTG ANGGNNNGNA AANNNNNNNN NN //
Primer: IRES-F (Pr0036) Region: IRES,HygR Sequence file: RDB09233_A7Ilf.seq
>09233_09233_1_A7Il_IRES-F_B07_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN GTCGAGGTTN NNAAACGTCT AGGCCCCCCG AACCNCGGGG    61 NCGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGAA AAAGCCTGAA   121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG   181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA   241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTCATCGG   301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG   361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA   421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC   481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT   541 ACGTGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG   601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC   661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG   721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGNNTCN   781 NAATACGANG TCGCCNACAT CTTCTTCTGG ANGCCGNGGT TGGCTTGTAT GGANCAGCAG   841 ACGCGCTACT TCNANCGGAN GCNTNCGGAN CTTGNNNNAT CGCCGCGGNN NCNGGCGTAT   901 ATGCTNNNNA TTNNNCTNGN CCNACTCTAT CNNANCTTNG NTNNACGGNN NTTTCNATGN   961 NGCNGCTTNN NNGCNNGNNC NANGNNNNGC NNNNNNNNNA TNCNGNNNNC NGNNNNNNNN 1021 NGNNNNNNNN NN //
Primer: IRES_R3 (Pr0573) Region: BstXI-stuffer Sequence file: RDB09233_A7Ilg.seq
>09233_09233_B3IjF_1_IRES_R3_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGNN NGGNNNNGGG GGGGGGGCGG NNNTCGNTAT CAAGCTTATC    61 GATACCGTCG ATCTCNNTGG TATTTGTGAG CCAGTTGGCC ACACCAGCCA CCACCTTCTG   121 ATAGGCAGCC TGCACCTGAG GAGTGAATTC CTCGAGATCC ATTGTGCTGG CGCGGATTCT   181 TTATCACTGA TAAGTTGGTG GACATATTAT GTTTATCAGT GATAAAGTGT CAAGCATGAC   241 AAAGTTGCAG CCGAATACAG TGATCCGTGC CGCCCTGGAC CTGTTGAACG AGGTCGGCGT   301 AGACGGTCTG ACGACACGCA AACTGGCGGA ACGGTTGGGG GTTCAGCAGC CGGCGCTTTA   361 CTGGCACTTC AGGAACAAGC GGGCGCTGCT CGACGCACTG GCCGAAGCCA TGCTGGCGGA   421 GAATCATACG CATTCGGTGC CGAGAGCCGA CGACGACTGG CGCTCATTTC TGATCGGGAA   481 TGCCCGCAGC TTCAGGCAGG CGCTGCTCGC CTACCGCCAG CACAATGGAT CTCGAGGAAT   541 TCTTTGCCAA AATGATGAGA CAGCACAATA ACCAGCACGT TGCCCAGGAG CTGTAGGAAA   601 AAGAAGAAGG CATGAACATG GTTANNNNAG GCTCTAGAGC CGCCGGTCAC ACGCCAGAAG   661 CCGAACCCCG CCCTGCCCCG TCCCCCCCGA AGGCAGCCGT CCCCCCGCGG ANAGCCCCGA   721 NGCTGGANAG GGAGAANGGG ANGGCGGCGC GGCNNNNCAC GANNGNCCNT CCCCGCCNAT   781 TTNCNTTCCT GCNNGCGCNG CACCGCTTCN CCCCGNGCCC GCTANANGGG GTGCGGCNGN   841 NNCCTNCCCN NANTTNCNGC NNCNNNNNAN NNTNNGGNNN NANNNGNANN NCCCNTGCGC 901 CCNTCTCNCN NGANNNNNNN NAAAANGGNA ANNGGCTGCN GNNNNNNNNN N //
Primer: CMV_en_R (Pr0173) Region: CAG pro,polyoma ori Sequence file: RDB09233_A7Ilh.seq
>09233_09233_1_A7Il_CMV_en_R_D07_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNNAACTAG TCAATAATCA ATGTCGACGA TGATATCAGA    61 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA   121 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG   181 CAATAAACAA GTTAATTCGA TTCAGCTTCA GAAGATGGCG GAGGGCCTCC AACACAGTAA   241 TTTTCCTCCC GACTCTTAAA ATAGAAAATG TCAAGTCAGT TAAGCAGGAA GTGACTAACT   301 GACCGCAGCT GGCCGTGCGA CATCCTCTTT TAATTAGTTG CTAGGCAACT GCCCTCCAGA   361 GGGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTCAG   421 TGTGGTTTTG CAAGAGGAAG CAAAAAGCCT CTCCACCCAG GCCTGGAATG TTTCCACCCA   481 ATCATTACTA TGACAACAGC TGTTTTTTTT AGTATTAAGC AGAGGCCGGG GGCCCCTGGC   541 CTCCGCTTAC TCTGGAGAAA AAGAAGAGAG GCTTCCAGAG GCAACTTGTC AAAACAGGAC   601 TGGCGCCTTG GAGGCGCTGT GGGGCCACCC AAATTGATAT AATTAAGCCC CAACCGCCTC   661 TTCCCGCCTC ATTTCAGCCT CACCACTATG TGAATATAGC TGAATACACA GTTTATTGAA   721 TAAACATTAA TTTCCAGGAA ATACAGTCTT TGTTTTTCCA AAGCGGTCAT TGAAAAAGGA   781 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC   841 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGANGCTGAA GANCAGTTGG   901 GNGNANGANN GGGTTNNNTC GAANNGGATC TCNNCAGCNG TAAGATCCNT NANANTTTTC 961 GCCCCGAANA ANGTTTTNNN NNGATGANCN NNTTTNANNN NNNGNTANNN NNNNNNN //
Primer: HygR-C_F (Pr0501) Region: HygR Sequence file: RDB09233_A7Ili.seq
>09233_09233_A7Il_HygR-C_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT TCTTCTGGAG GCNNNNNNTG    61 GCTTGTATGG AGCAGCAGAC GCGCTACTTC NAGCGGAGGC ATCCGGAGCT TGCAGGATCG   121 CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT   181 GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC   241 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT   301 GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA   361 AAGGAATAGT CGAGAAATTG ATGATCTATT AAGCAATAAN GACGTCCACT AAAATGGAAG   421 TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA   481 GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTANAT NAATGNNNGN NCNNNNNNGN   541 NNGNTCNTNN NNNNTGNNNN NNGANANTGN TNCANNNNNN NANNNNANNN NTNNNNNANN 601 CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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