Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-LIF (#RDB09182)

Expression clone of human LIF.

Alternative name stock no 108
Clone info. Expression clone of human LIF.
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Gene/insert name Human LIF cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB09182z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
MTAに書く付加的使用条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09182 pCAGGS-LIF DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-LIF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09182).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09182_A5Gtp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: insert 5'
Sequence file: RDB09182_A5Gtc.seq check
>09182_09182_A5Gt_1_pAxCA_F1_E04_13.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNNNNGC CTTCTNNTTT TNCCNNCNGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGC TGCAGAGCTC
  121 CGGCATCTGA GGTTTCCTCC AAGGCCCTCT GAAGTGCAGC CCATAATGAA GGTCTTGGCG
  181 GCAGGAGTTG TGCCCCTGCT GTTGGTTCTG CACTGGAAAC ATGGGGCGGG GAGCCCCCTC
  241 CCCATCACCC CTGTCAACGC CACCTGTGCC ATACGCCACC CATGTCACAA CAACCTCATG
  301 AACCAGATCA GGAGCCAACT GGCACAGCTC AATGGCAGTG CCAATGCCCT CTTTATTCTC
  361 TATTACACAG CCCAGGGGGA GCCGTTCCCC AACAACCTGG ACAAGCTATG TGGCCCCAAC
  421 GTGACGGACT TCCCGCCCTT CCACGCCAAC GGCACGGAGA AGGCCAAGCT GGTGGAGCTG
  481 TACCGCATAG TCGTGTACCT TGGCACCTCC CTGGGCAACA TCACCCGGGA CCAGAAGATC
  541 CTCAACCCCA GTGCCCTCAG CCTCCACAGC AAGCTCAACG CCACCGCCGA CATCCTGCGA
  601 GGCCTCCTTA GCAACGTGCT GTGCCGCCTG TGCAGCAAGT ACCACGTGGG CCATGTGGAC
  661 GTGACCTACG GCCCTGACAC CTCGGGTAAG GATGTCTTCC AGAAGAAGAA GCTGGGCTGT
  721 CAACTCCTGG GGAAGTATAA GCAGATCATC GCCGTGTTGG CCCAGGCCTT CTAGCAGGAG
  781 GTCTTGAAGT GTGCTGTGAA CCGANGGATC TCAGCTGCAG AGCTAGCTCT GCAGCTCGAN
  841 GAATTCANTC CTCANGTGCA GGCTGCCTAT CAGAANGTGG TGGCTGGTGT GGCCAATGCC
  901 CTGGCTCACA AATACNACNN ANNTCNTTTT TCCNNCTGCC NAAANNTNTN GGGGNNNTCA
961 TGAAGCNNNN NNANNATCNN NNNNNNGGCN NNTAANNGAA ATTTNTTTTN NNNNNNNNN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin p A,insert 3'
Sequence file: RDB09182_A5Gtd.seq check
>09182_09182_B1G2F_pCAGGS_R1_H05_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT TGNNNTGNAN ATAAATTTCC TTTATTAGCC AGAAGTCAGA
   61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCTCAGTGG
  121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC
  181 TGAGGAGTGA ATTCCTCGAG CTGCAGAGCT AGCTCTGCAG CTGAGATCCC TCGGTTCACA
  241 GCACACTTCA AGACCTCCTG CTAGAAGGCC TGGGCCAACA CGGCGATGAT CTGCTTATAC
  301 TTCCCCAGGA GTTGACAGCC CAGCTTCTTC TTCTGGAAGA CATCCTTACC CGAGGTGTCA
  361 GGGCCGTAGG TCACGTCCAC ATGGCCCACG TGGTACTTGC TGCACAGGCG GCACAGCACG
  421 TTGCTAAGGA GGCCTCGCAG GATGTCGGCG GTGGCGTTGA GCTTGCTGTG GAGGCTGAGG
  481 GCACTGGGGT TGAGGATCTT CTGGTCCCGG GTGATGTTGC CCAGGGAGGT GCCAAGGTAC
  541 ACGACTATGC GGTACAGCTC CACCAGCTTG GCCTTCTCCG TGCCGTTGGC GTGGAAGGGC
  601 GGGAAGTCCG TCACGTTGGG GCCACATAGC TTGNCNNGGT TGNTGNNGAA CGGCTCCCCN
661 NNNNTGNGTN NNNNANAANA AAGAGGGNAN TGNCACTGNC ANTNNNNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Tsuyama, J., MicroRNA-153 Regulates the Acquisition of Gliogenic Competence by Neural Stem Cells. Stem Cell Reports 5 (3): 365-377 (2015). PMID 26212661. [link to RRC of NBRP]