Resource data sheet
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pCAGGS-LIF

Expression clone of human LIF.

Catalog number RDB09182
Resource name pCAGGS-LIF
Alternative name stock no 108
Clone info. Expression clone of human LIF.
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Gene/insert name Human LIF cDNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank human LIF (NCBI Gene 3976) |

Distribution information

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Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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RDB09182 pCAGGS-LIF DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB09182z.seq from Depositor
Electronic file Map RDB09182a.gif from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB09182_A5Gt.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB09182_A5Gta.seq
>09182_09182_A5Gt_1_pAxCA_F1_E04_13.ab1
    1 TGTCGATGCA ACATCTTCTC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGC TGCAGAGCTC
  121 CGGCATCTGA GGTTTCCTCC AAGGCCCTCT GAAGTGCAGC CCATAATGAA GGTCTTGGCG
  181 GCAGGAGTTG TGCCCCTGCT GTTGGTTCTG CACTGGAAAC ATGGGGCGGG GAGCCCCCTC
  241 CCCATCACCC CTGTCAACGC CACCTGTGCC ATACGCCACC CATGTCACAA CAACCTCATG
  301 AACCAGATCA GGAGCCAACT GGCACAGCTC AATGGCAGTG CCAATGCCCT CTTTATTCTC
  361 TATTACACAG CCCAGGGGGA GCCGTTCCCC AACAACCTGG ACAAGCTATG TGGCCCCAAC
  421 GTGACGGACT TCCCGCCCTT CCACGCCAAC GGCACGGAGA AGGCCAAGCT GGTGGAGCTG
  481 TACCGCATAG TCGTGTACCT TGGCACCTCC CTGGGCAACA TCACCCGGGA CCAGAAGATC
  541 CTCAACCCCA GTGCCCTCAG CCTCCACAGC AAGCTCAACG CCACCGCCGA CATCCTGCGA
  601 GGCCTCCTTA GCAACGTGCT GTGCCGCCTG TGCAGCAAGT ACCACGTGGG CCATGTGGAC
  661 GTGACCTACG GCCCTGACAC CTCGGGTAAG GATGTCTTCC AGAAGAAGAA GCTGGGCTGT
  721 CAACTCCTGG GGAAGTATAA GCAGATCATC GCCGTGTTGG CCCAGGCCTT CTAGCAGGAG
  781 GTCTTGAAGT GTGCTGTGAA CCGAGGGATC TCAGCTGCAG AGCTAGCTCT GCAGCTCGAG
  841 GAATTCACTC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC
  901 CTGGCTCACA AATACCACTG AGATCTTTTT TCCCTCTGCC AAAAATTATG GGGGACATCA
  961 TGAAGCCCCT TGAGCATCTG ACTTCTGGCT AATAAAGGAA ATTTATTTTC ATTGCAATAG
 1021 TGTGTTGGAA ATTTTTTTGT GTCTCTCACT CGGAAGACAT ATGGGAGGGG CAAATCATTT
 1081 AAAAACATCA GAAATTGAAG TATTTGCTTA GAGTTTGGCT ACATATGCCC CATATTGCTG
1141 GGCCTGACCA ATTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

reference Tsuyama, J., MicroRNA-153 Regulates the Acquisition of Gliogenic Competence by Neural Stem Cells. Stem Cell Reports 5 (3): 365-377 (2015). PMID 26212661.

2018.12.10

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