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pUC-GFPS1 (#RDB09059)

Plasmid clone of GFPS1, a GFP mutation variant


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Seki, E., Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name GFP mutation variant
Clone info. GFPS1 gene with a T7 promoters in a pUC19 vector. GFPS1 is a GFP mutation variant, with F46L, S65T, F99S, M153T, V163A, Y200C, to enhance the brightness and the maturation speed of GFP. Fluorescence can be detected by a fluorescence plate reader with excitation at 485 nm and emission at 507 nm (Seki, E., Anal. Biochem. 377 (2) 156-161, 2008).
Vector backbone pUC19 (Plasmid)
Size of vector backbone 3.3 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kigawa, Takanori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR is requested (Anal.Biochem.377,156-161(2008)). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.
Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用する (Anal.Biochem.377,156-161(2008))。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。
備考 非営利機関の非営利学術研究に限って提供する。

Catalog # Resource name Shipping form Fee
RDB09059 pUC-GFPS1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC-GFPS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09059).

Reference section:

Seki, E., Matsuda, N., Yokoyama, S., Kigawa, T., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Seki, E., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [PubMed] [Article] [RRC of NBRP]