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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC-GFPS1 (#RDB09059)

Plasmid clone of GFPS1, a GFP mutation variant

Alternative name GFP mutation variant
Clone info. GFPS1 gene with a T7 promoters in a pUC19 vector. GFPS1 is a GFP mutation variant, with F46L, S65T, F99S, M153T, V163A, Y200C, to enhance the brightness and the maturation speed of GFP. Fluorescence can be detected by a fluorescence plate reader with excitation at 485 nm and emission at 507 nm (Seki, E., Anal. Biochem. 377 (2) 156-161, 2008).
Vector backbone pUC19 (Plasmid)
Size of vector backbone 3.3 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kigawa, Takanori |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR is requested (Anal.Biochem.377,156-161(2008)). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.
Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用する (Anal.Biochem.377,156-161(2008))。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。
備考 非営利機関の非営利学術研究に限って提供する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09059 pUC-GFPS1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC-GFPS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09059).

Reference section:

Seki, E., Matsuda, N., Yokoyama, S., Kigawa, T., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09059_B2B3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB09059_B2B3a.seq check
>09059_09059_B2B3_3_Amp_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNA CATATNNNNN NNNATTTAGA AAAATAAACA AATAGGGGTT
   61 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA
  121 TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC
  181 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT
  241 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG
  301 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC GCTCTTGTCA TTGTGCTTCG
  361 CATGATTACG AATTCAGATC TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGAGACC
  421 ACAACGGTTT CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGAA
  481 AAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA
  541 TGTTAATGGG CACAAATTTT CTGTCNGTGG AGAGGGTGAA GGTGANGCAA CATACGGAAA
  601 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCNTGGC CAANNCNTGT
661 CNCTACNTNG ACNTATGGNG NNNNANGNNT TTCCNNNNNN NNNN
//
Primer: lacIpro-B_F
Sequence file: RDB09059_B2B3b.seq check
>09059_09059_B2B3_3_lacIpro-B_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTGCTCTT GTCATTGTGC TTCGCCAAGC
  121 TTGCATGCCT GCAGCTCGAG GTTATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT
  181 CAAGACCCGT TTAGAGGCCC CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC
  241 CAACTCAGCT GGGCGGGGAT CAATCAATCA TTATTATTTG TAGAGCTCAT CCATGCCATG
  301 TGTAATCCCA GCAGCAGTTA CAAACTCAAG AAGGACCATG TGGTCACGCT TTTCGTTGGG
  361 ATCTTTCGAA AGGGCAGATT GTGTCGACAG GCAATGGTTG TCTGGTAAAA GGACAGGGCC
  421 ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGGAAC CATCTTCAAT
  481 GTTGTGGCGA ATTTTGAAGT TAGCTTTGAT TCCATTCTTT TGTTTGTCTG CCGTGATGTA
  541 TACATTGTGT GAGTTATAGT TGTACTCGAG TTTGTGTCCG AGAANGTTTC CNTCTTCTTT
  601 AANATCNATA CCTTTTAACT CGATACGATT AACAAGGGTA TCACCTTCAA ACTTGACTTC
661 NGCACGCGTC TTGNANTNCC CGTCNTCNTN NNNNNNNNN
//
Primer: T7_20bp
Sequence file: RDB09059_B2B3c.seq check
>09059_09059_B2B3_3_T7_20bp_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNTNNNNNN NNNNNTAAGA AGGAGANATA CATATGAAAA GTAAAGGAGA
   61 AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA
  121 CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA
  181 ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGCCA ACACTTGTCA CTACTTTGAC
  241 TTATGGTGTT CAATGCTTTT CCCGTTATCC GGATCACATG AAACGGCATG ACTTTTTCAA
  301 GAGTGCCATG CCCGAAGGTT ATGTACAGGA ACGCACTATA TCTTTCAAAG ATGACGGGAA
  361 CTACAAGACG CGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATC GTATCGAGTT
  421 AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTCGGA CACAAACTCG AGTACAACTA
  481 TAACTCACAC AATGTATACA TCACGGCAGA CAAACAAAAG AATGGAATCA AAGCTAACTT
  541 CAAAATTCGC CACAACATTG AAGATGGTTC CGTTCAACTA GCAGANCATT ATCAACAAAA
  601 TACTCCNATT GNCGATGGNC CTGTCCNTTT ANCANACNAC CATTGCCTGT CNACNCAATC
661 TGNCCTTTCN AAAGNNCCNN NCNAAAANCG NGNNNNCNNG GTCCNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Seki, E., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [link to RRC of NBRP]