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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC-GFPS1

Plasmid clone of GFPS1, a GFP mutation variant

Catalog number RDB09059
Resource name pUC-GFPS1
Alternative name GFP mutation variant
Clone info. GFPS1 gene with a T7 promoters in a pUC19 vector. GFPS1 is a GFP mutation variant, with F46L, S65T, F99S, M153T, V163A, Y200C, to enhance the brightness and the maturation speed of GFP. Fluorescence can be detected by a fluorescence plate reader with excitation at 485 nm and emission at 507 nm (Seki, E., Anal. Biochem. 377 (2) 156-161, 2008).
Vector backbone pUC19 (Plasmid)
Size of vector backbone 3.3 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kigawa, Takanori |
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External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR is requested (Anal.Biochem.377,156-161(2008)). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用する (Anal.Biochem.377,156-161(2008))。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考 非営利機関の非営利学術研究に限って提供する。
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09059 pUC-GFPS1 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Seki, E., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09059_B2B3p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Amp_R
            Region: AmpR pro,insert 5'(T7 pro,RBS.GFPS1 5')
            Sequence file: RDB09059_B2B3a.seq check
            >09059_09059_B2B3_3_Amp_R_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNA CATATNNNNN NNNATTTAGA AAAATAAACA AATAGGGGTT
               61 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA
              121 TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC
              181 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT
              241 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG
              301 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC GCTCTTGTCA TTGTGCTTCG
              361 CATGATTACG AATTCAGATC TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGAGACC
              421 ACAACGGTTT CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGAA
              481 AAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA
              541 TGTTAATGGG CACAAATTTT CTGTCNGTGG AGAGGGTGAA GGTGANGCAA CATACGGAAA
              601 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCNTGGC CAANNCNTGT
            661 CNCTACNTNG ACNTATGGNG NNNNANGNNT TTCCNNNNNN NNNN
            //
            Primer: lacIpro-B_F
            Region: insert 3'(T7 ter,GFPS1 3')
            Sequence file: RDB09059_B2B3b.seq check
            >09059_09059_B2B3_3_lacIpro-B_F_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC
               61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTGCTCTT GTCATTGTGC TTCGCCAAGC
              121 TTGCATGCCT GCAGCTCGAG GTTATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT
              181 CAAGACCCGT TTAGAGGCCC CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC
              241 CAACTCAGCT GGGCGGGGAT CAATCAATCA TTATTATTTG TAGAGCTCAT CCATGCCATG
              301 TGTAATCCCA GCAGCAGTTA CAAACTCAAG AAGGACCATG TGGTCACGCT TTTCGTTGGG
              361 ATCTTTCGAA AGGGCAGATT GTGTCGACAG GCAATGGTTG TCTGGTAAAA GGACAGGGCC
              421 ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGGAAC CATCTTCAAT
              481 GTTGTGGCGA ATTTTGAAGT TAGCTTTGAT TCCATTCTTT TGTTTGTCTG CCGTGATGTA
              541 TACATTGTGT GAGTTATAGT TGTACTCGAG TTTGTGTCCG AGAANGTTTC CNTCTTCTTT
              601 AANATCNATA CCTTTTAACT CGATACGATT AACAAGGGTA TCACCTTCAA ACTTGACTTC
            661 NGCACGCGTC TTGNANTNCC CGTCNTCNTN NNNNNNNNN
            //
            Primer: T7_20bp
            Region: insert mid(GFPS1)
            Sequence file: RDB09059_B2B3c.seq check
            >09059_09059_B2B3_3_T7_20bp_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNTNNNNNN NNNNNTAAGA AGGAGANATA CATATGAAAA GTAAAGGAGA
               61 AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA
              121 CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA
              181 ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGCCA ACACTTGTCA CTACTTTGAC
              241 TTATGGTGTT CAATGCTTTT CCCGTTATCC GGATCACATG AAACGGCATG ACTTTTTCAA
              301 GAGTGCCATG CCCGAAGGTT ATGTACAGGA ACGCACTATA TCTTTCAAAG ATGACGGGAA
              361 CTACAAGACG CGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATC GTATCGAGTT
              421 AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTCGGA CACAAACTCG AGTACAACTA
              481 TAACTCACAC AATGTATACA TCACGGCAGA CAAACAAAAG AATGGAATCA AAGCTAACTT
              541 CAAAATTCGC CACAACATTG AAGATGGTTC CGTTCAACTA GCAGANCATT ATCAACAAAA
              601 TACTCCNATT GNCGATGGNC CTGTCCNTTT ANCANACNAC CATTGCCTGT CNACNCAATC
            661 TGNCCTTTCN AAAGNNCCNN NCNAAAANCG NGNNNNCNNG GTCCNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Seki, E., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196.

            2022.03.02

            GNP_filter3_RDBDEP_html_220225.pl