RDB09058 - pUC-CAT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pUC-CAT (#RDB09058)

Chloramphenicol acetyltransferase (CAT ) gene with a T7 promoter

Clone info.	Chloramphenicol acetyltransferase (CAT ) gene with a T7 promoter site in a pUC vector.
Vector backbone	pUC19 (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	E. coli Chloramphenicol acetyltransferase genomic DNA
Depositor\|Developer	Kigawa, Takanori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とする。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって謝辞の表明を必要とする。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB09058	pUC-CAT	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC-CAT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09058).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB09058_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 Sequence file: RDB09058_A6Jca.seq
>09058_09058_A6Jc_1_Reverse2_A03_01_ABI08.ab1 1 CAGGGACATG ATACGATTCA GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA 61 GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA 121 TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC 181 ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA 241 TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC 301 ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG 361 AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA 421 CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT 481 CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA 541 ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG 601 CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG 661 ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTTTGTGAT GGCTTCCATG 721 TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAT 781 TTTTTTAAGG CAGTTATTGG TGCCCTTAAA CGTCGACCGG CTGCTAACAA AGCCCGAAAG 841 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 901 AAACGGGTCT TGAGGGGTTT TTTGCTGAAG GAGGAACTAT ATCCGGATAA CCTCGAGCTG 961 CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG GAAAACCCTG 1021 CGCTGCATTT AATGGATCGA CACGCGCGGG GAGGAGGCGG ATTGCGTATG GGCGCTCATC 1081 GCTTCTCGCC TCACTGACTC GCTTGCGCCT CGGATCGATT CGGGCCTTGC GGCCGAGACG //

Primer: Reverse2
Sequence file: RDB09058_A6Jca.seq check

>09058_09058_A6Jc_1_Reverse2_A03_01_ABI08.ab1
    1 CAGGGACATG ATACGATTCA GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA
   61 GACCACAACG GTTTCCCTCT AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA
  121 TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC
  181 ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA
  241 TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC
  301 ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG
  361 AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA
  421 CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT
  481 CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA
  541 ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG
  601 CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG
  661 ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTTTGTGAT GGCTTCCATG
  721 TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAT
  781 TTTTTTAAGG CAGTTATTGG TGCCCTTAAA CGTCGACCGG CTGCTAACAA AGCCCGAAAG
  841 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
  901 AAACGGGTCT TGAGGGGTTT TTTGCTGAAG GAGGAACTAT ATCCGGATAA CCTCGAGCTG
  961 CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG GAAAACCCTG
1021 CGCTGCATTT AATGGATCGA CACGCGCGGG GAGGAGGCGG ATTGCGTATG GGCGCTCATC
1081 GCTTCTCGCC TCACTGACTC GCTTGCGCCT CGGATCGATT CGGGCCTTGC GGCCGAGACG
//

Please visit Sequencing and PCR primers for primer information.