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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGFPS1 (#RDB09057)

Plasmid clone of GFPS1, a GFP mutation variant

Alternative name GFP mutation variant
Clone info. GFPS1 gene with a T7 promoter and terminator. GFPS1 is a GFP mutation variant, with F46L, S65T, F99S, M153T, V163A, Y200C, to enhance the brightness and the maturation speed of GFP. Fluorescence can be detected by a fluorescence plate reader with excitation at 485 nm and emission at 507 nm (Seki, E., Anal. Biochem. 377 (2) 156-161, 2008).
Vector backbone pCR2.1-TOPO (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r, Kan^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kigawa, Takanori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR is requested (Anal.Biochem.377,156-161(2008)). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the BIOLOGICAL RESOURCE, or the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT must contact the DEPOSITOR in the case of transfer, sell, or assign the BIOLOGICAL RESOURCE, or the products derived from the BIOLOGICAL RESOURCE, to any third party.
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用する (Anal.Biochem.377,156-161(2008))。利用者が本件リソース及び本件リソースを使用して得られた研究成果に基づき特許等の申請、又は事業活動を行う場合は、寄託者と別途協議を行う。利用者が本件リソース及び本件リソースに由来する産物を第三者へ移転、譲渡または分与する場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
非営利機関の非営利学術研究に限って提供する。
備考 非営利機関の非営利学術研究に限って提供する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09057 pGFPS1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGFPS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09057).

Reference section:

Seki, E., Matsuda, N., Yokoyama, S., Kigawa, T., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09057_B2F1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro.1,T7 pro.2,insert 5'
Sequence file: RDB09057_B2F1a.seq check
>09057_09057_B2F1_3_pUC-M13_-100_B08_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC CNGGGTTTTC CCNGTCACGA CGTTGTAAAA
   61 CGACGGCCAG TGAATTGNNN NNNNACTCAC TATAGGGCGA ATTGGGCCCT CTAGATGCAT
  121 GCTCGAGCGG CCGCCAGTGT GATGGATATC TGCAGAATTC GCCCTTGCTC TTGTCATTGT
  181 GCTTCGCATG ATTACGAATT CAGATCTCGA TCCCGCGAAA TTAATACGAC TCACTATAGG
  241 GAGACCACAA CGGTTTCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA
  301 TATGAAAAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA
  361 TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA
  421 CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGCCAAC
  481 ACTTGTCACT ACTTTGACTT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA
  541 ACGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC
  601 TTTCAAAGAT GACGGGAACT ACAAGACGCG TGCTGAAGTC AAGTTTGAAG GTGATACCCT
  661 TGTTAATCGT ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTCGGACA
  721 CAAACTCGAG TACAACTATA ACTCACACAA TGTATACATC ACGGCAGACA AACAAAAAGA
  781 NNGGAATCAA AGCTAACTTC NAAATTCNNC ACAACATTGN AAGANGGTTC CGTTCAACTA
  841 GCAGANCATT ATCAACNAAA TACTCCNANT TGGCGNNGGN CCTGTNCTTT TANCAGACAN
  901 NCATTGNNNG TCNACNNAAT CTGCCNTTNC NAANNATCCC NACGAAAAAG CGTGANCACN
  961 NNGNNNNTTN NTGNNTTNGN AACTGNTGCT GGGNNTNCNC NTNGNNNNGN NTNANCNCNA
1021 NNANNAANGA ANTANNTNCN ANNNGANCNN NNNNNGNNNG NNNNNNNNNN
//
Primer: reverse-2 (Pr0100)
Region: T7 ter,insert 3'
Sequence file: RDB09057_B2F1b.seq check
>09057_09057_B2F1_3_reverse-2_A08_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCTTGGTANC GAGCTCGGNN CCNCTAGTAA CGGCCGCCAG
   61 TGTGCTGGAA TTCGCCCTTG CTCTTGTCAT TGTGCTTCGT ATAAAAATAG GCGTATCACG
  121 AGGCCCTTTC GTCTTCAAGA ATTATCCGGA TATAGTTCCT CCTTTCAGCA AAAAACCCCT
  181 CAAGACCCGT TTAGAGGCCC CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC
  241 CAACTCAGCT TCCTTTCGGG CTTTGTTAGC AGCCGGATCT GCTCGAGCGG CCGCCAGTGT
  301 GATGGATTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT CGGGCTTTGT
  361 TTAGCAGCCT AGGTATTAAT CAATTAGTGG TGGTGGTGGT GGTGGTGGTG CTCGAGTGCG
  421 GCCGCAAGCT TGCTAGCGGC GCTCAGTTGG AATTAATTCA TTATTTGTAG AGCTCATCCA
  481 TGCCATGTGT AATCCCAGCA GCAGTTACAA ACTCAAGAAG GACCATGTGG TCACGCTTTT
  541 CGTTGGGATC TTTCGAAAGG GCAGATTGTG TCGACAGGCA ATGGTTGTCT GGTAAAAGGA
  601 CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGGAACCAT
  661 CTTCAATGTT GTGGCGAATT TTGAAGTTAG CTTTGATTCC ATTCTTTTGT TTGTCTGCCG
  721 TGATGTATAC ATTGTGTGAG TTATAGTTGT ACTCGAGTTT GTGTCCGAGA ATGTTTCCAT
  781 CTTCTTNNAA ATCAATACCN TTTAACTCGA TACGATTAAC AAGGGTATCA CCTTCAAACT
  841 TGACTTCAGC ACGCGTCTTG NAGTTNCCGT CATCTTTGAA ANANATANTG CGTTCCTGTA
  901 CATAACCTTC NGGGCATGNN ACTCTTGAAA AAGTCNTGCC GTTTCATGTG ATCCGGANAA
  961 CGGGAAAGCN TTNNAACACC ATAAGTNAAA GTANTGNNNN NNGNTNGNNN NNNNNANNNN
1021 GNNANNTNNN NNNNNNN
//
Primer: phBG_F (Pr0916)
Region: KanR
Sequence file: RDB09057_B2F1c.seq check
>09057_09057_B2F1_phBG_F_E03_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTGNCNN NNNNNNCTGN NGGCGCAGGG GNNCAAGATC
   61 TGATCANNGA GACAGGATGA NGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  121 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  181 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  241 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  301 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  361 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCCCAC CTTGCTCCTG
  421 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  481 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  541 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  601 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
  661 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NNNNACTGTG
  721 GCCGGCTGGG TGTGGCGGAC CGCTATCNGG ACATAGCGTT GGCTACCCGT GATATTGCTG
  781 ANNAGCTTGG CGGCNAATGG GCTGACCGCT TCCTCGTGCT TTANNGTATC NCCGCTCNNG
  841 ATTCNCAGCG CATCGCCTTC NNNNNNNNTC TTGACGAGNT NCTTCTGANN NNAANANNGA
  901 AGANTATGAG TATTCANNAT TTCCGTGTCG CCCNTNNNNN CCTTTTTNNG GCATTTTGCC
  961 TTNNNNNTTT TGNTCNCCNN NAAANNGCNG GNNGAAAGTA AAAGANGCTG AAANNNNNNN
 1021 TNNGNNNNNN CNANTGGGNN NNNTNNAACT NGNANNNTCN NNNNCNGNNN NNNNNNCNTN
1081 NNNNTTTNNN NNCCNANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Seki, E., Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Anal. Biochem., 377(2): 156-161 (2008). PMID 18375196. [link to RRC of NBRP]