Resource data sheet
Prev.

pMT-PURO2R

Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells

Catalog number RDB09005
Resource name pMT-PURO2R
Clone info. Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Discosoma sp. red fluorescent protein cDNA
Depositor Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA [Link]
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
Please use the Exclusive MTA (Clontech FPs).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09005 pMT-PURO2R DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB09005z.seq from Depositor

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB09005_A3I6.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: V5_Reverse RDB09005_A3I6a.seq
>09005_09005_A3I6_V5_Reverse_B03_06.ab1
    1 CATCGAGGCT CTAGACTTAA GCCTAGGTGT ACACACGTGG ATATCCGGAC TAGTTCGCGA
   61 AGATCTCCCG AGCGAGAGGC CAACAAAGGC CACGACGGCC AGTAATATGC ATAACTTCAT
  121 ATTGAGATCG GATCCCCCCT TTAGTTGCAC TGAGATGATT CACTTGATTT TGCTGCTGAC
  181 CACAACTGAT GCACATTGGC CAGATGCTCT CGGTTTTATA CATTTTGGAA ACGCGGCTTT
  241 ACACACGGGT CTTTCGTTCG ATTCACCTCT TTTGCACACG CCGGCCGGCT CCAAATTCGA
  301 GTGGAGACGT GTGCAGAAGC TTTTGTTCTG CCCGCGAAGA ATTGGTTCGG TTCTCCACTT
  361 TGGGGACTTG TATGGGATGC AAAGCTCTTG GTGGGGCACA TGGGGCATGC GCAGGCACTC
  421 TTACTTGCGT ACCACATATG TATGGGGGTG GCGGTGGGCG GTGGGGGGCC GGAGTTCTGG
  481 GTCTCTTATC GGAACCAGAG GATAGGACGG CCTGCAGCAA AGAGCTAGTC ACATCGGGCA
  541 CCACATCCTG TCCTGCAACG AATTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG
  601 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG
  661 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
  721 GAATGGCGCT AGAGGATCCG GTGCGTGGTG GTTTCATGCT TCTGGGAACG GCAAATGGGT
  781 TTAGGATTGG GAACCCCTCA TCATCTGTTG GAATATACTA TTCAACCTAC AAAAGTAACG
  841 TTAAACAACA CTACTTTATA TTTGATATGA ATGGCCACAC CTTTTATGCC ATAAAACATA
  901 TTGTAAGAGA ATACCACTCT TTTTATTCCT TCTTTCCTTC TTGTACGTTT TTTGCTGTAA
  961 GTAGGTCGTG GTGCTGGTGT TGCAGTTGAA TAACTTAAAA TATAAATCAT AAAACTCAAC
 1021 ATAAACTTTG ACTATTTATT TATTTATTAA GAAGGAAATA TAATTATAAT TTACACAGAT
 1081 CCCCCGGCTG CAGGAATTCG AATAGCTGCG CGCCACATGA ACGAGTACAG GCCCACGATG
1141 CGCCTCGGTC CAACCCGCCG GAACCGAACG T
//
Primer: Amp_R RDB09005_A3I6b.seq
>09005_09005_A3I6_Amp_R_A03_03.ab1
    1 CTTGTTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
  241 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG
  301 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA
  361 TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
  421 ACCGCATCAG GCGCCGGTAC CCGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA
  481 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
  541 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTCAA GCTAGCTGGC CTCGAGGTCG
  601 ACGGTATCGA TAAGCTTGAT CTAGAGTCGG TGGGCCTCCT ACAGGAACAG GTGGTGGCGG
  661 CCCTCGGTGC GCTCGTACTG CTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC
  721 AGCTTGGCGT CCACGTAGTA GTAGCCGGGC AGCTGCACGG GCTTCTTGGC CATGTAGATG
  781 GACTTGAACT CCACCAGGTA GTGGCCGCCG TCCTTCAGCT TCAGGGCCTT GTGGGTCTCG
  841 CCCTTCAGCA CGCCGTCGCG GGGGTACAGG CGCTCGGTGG AGGCCTCCCA GCCCATGGTC
  901 TTCTTCTGCA TCACGGGGCC GTCGGAGGGG AAGTTCACGC CGATGAACTT CACCTTGTAG
  961 ATGAAGCAGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG TCGCCACGCC GCCGTCCTCG
 1021 AAGTTCATCA CGCGCTCCAC TTGAAGCCCT CGGGGAGACA GCTTCTTGTA GTCGGGATGT
 1081 CGCGGGTGCT TTCACGTACA CAGCAGCGTA CTGGAACTGG GGGGAACAGG GATGTTCCCA
1141 AGGGCGTAAG GTCCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl