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pMT-PURO2R

Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells

Catalog number RDB09005
Resource name pMT-PURO2R
Clone info. Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Discosoma sp. red fluorescent protein cDNA
Depositor|Developer Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
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Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09005 pMT-PURO2R DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB09005zzk02.seq

Original reference

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18945_B1Cup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mRFP1-inner1
Region: DsRed2 3'
Sequence file: RDB18945_B1Cua.seq check
>09005_18945_B1Cu_3_mRFP1-inner1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNNN GNNTCCTCCC TGCAGGACGG CTGCTTCNTC TACAAGGTGA
   61 AGTTCATCGG CGTGAACTTC CCCTCCGACG GCCCCGTGAT GCAGAAGAAG ACCATGGGCT
  121 GGGAGGCCTC CACCGAGCGC CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGACCCACA
  181 AGGCCCTGAA GCTGAAGGAC GGCGGCCACT ACCTGGTGGA GTTCAAGTCC ATCTACATGG
  241 CCAAGAAGCC CGTGCAGCTG CCCGGCTACT ACTACGTGGA CGCCAAGCTG GACATCACCT
  301 CCCACAACGA GGACTACACC ATCGTGGAGC AGTACGAGCG CACCGAGGGC CGCCACCACC
  361 TGTTCCTGTA GGAGGCCCAC CGACTCTAGA TCAAGCTTAT CGATACCGTC GACCTCGAGG
  421 CCAGCTAGCT TGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
  481 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
  541 ATCAATGTAT CTTATCATGT CTGGATCGGG TACCGGCGCC TGATGCGGTA TTTTCTCCTT
  601 ACGCATCTGT GCGGNATTTN NCACCGCATA TGGTGNACTC TCNGTACAAT CTGCTCTGAN
  661 GNCGCANANN TAANCNNGCC CCNNNNCCNG CCNNCNCCNN CTGNNNNNNC NNNNNNGGNN
721 NGNNNNNNNC NNNNNNNNNN
//
Primer: PuroR_F
Region: PuroR, DsRed2 5'
Sequence file: RDB18945_B1Cub.seq check
>09005_18945_B1Cu_3_PuroR_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAAN GANCGCGCAC CTGNGTGCAT GACCCGCAAG
   61 CCCGGTGCCA TGGCCTCCTC CGAGAACGTC ATCACCGAGT TCATGCGCTT CAAGGTGCGC
  121 ATGGAGGGCA CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC
  181 TACGAGGGCC ACAACACCGT GAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC
  241 TGGGACATCC TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC
  301 GACATCCCCG ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG
  361 AACTTCGAGG ACGGCGGCGT GGCGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTGC
  421 TTCATCTACA AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTGATGCAG
  481 AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG
  541 AANGGCGAGA NCCACAAGGC CCTGAAGCTG ANNGACGGCG GNCACTACCT GGTGGAGTTC
  601 NAGTCCATCT ACNTGGCCNA GAANNCCGTG CAGCTGNCCG GCTNCTACTA CNTGGACGCC
  661 NAGCTGGANN TCNCCTCCCN CNACGAGNAN TACNNNTNNN GGNNNANTAC NNNNGCNNNN
721 ANGNNNNNCN NNNNNNGNNN NNNNNNN
//
Primer: COPIApro-F
Region: copia pro, PuroR
Sequence file: RDB18945_B1Cuc.seq check
>09005_18945_B1Cu_3_COPIApro-F_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNCTTTNN NTTCCTTCTT TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG
   61 GTCGTGGTGC TGGTGTTGCA GTTGAAATAA CTTAAAATAT AAATCATAAA ACTCAAACAT
  121 AAACTTGACT ATTTATTTAT TTATTAAGAA AGGAAATATA AATTATAAAT TACAACAGAT
  181 CCCCCGGGCT GCAGGAATTC GATAGCTTGC GCCGCCACCA TGACCGAGTA CAAGCCCACG
  241 GTGCGCCTCG CCACCCGCGA CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC
  301 GCCGACTACC CCGCCACGCG CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC
  361 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG
  421 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG
  481 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA
  541 CAGATGGAAG GNCTCCTGGC GCCGCACCGG CCCAANGAGC CCGCGTGGTT CCTGGCCACC
  601 GTCNGCGTCT CGNCCGACCA CCAGGGCAAG GNNNNNGGNN GCGCCNNCNN GNTCCCCGGA
  661 NNNNANNNGG NCGANCGCGN CNGGGTGNCC GNNNTNNNGN NNNNNTNCNN NNCCNNNNAN
721 NTCCCNNTNN NNNNNNNN
//
Primer: HB-NOS_R
Region: MT pro, BiP signal sequence
Sequence file: RDB18945_B1Cud.seq check
>09005_18945_B1Cu_3_HB-NOS_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTTG GGTANCGCCN GGGTTTTCCC NGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AATTAATTCG TTGCAGGACA GGATGTGGTG CCCGATGTGA CTAGCTCTTT
  121 GCTGCAGGCC GTCCTATCCT CTGGTTCCGA TAAGAGACCC AGAACTCCGG CCCCCCACCG
  181 CCCACCGCCA CCCCCATACA TATGTGGTAC GCAAGTAAGA GTGCCTGCGC ATGCCCCATG
  241 TGCCCCACCA AGAGCTTTGC ATCCCATACA AGTCCCCAAA GTGGAGAACC GAACCAATTC
  301 TTCGCGGGCA GAACAAAAGC TTCTGCACAC GTCTCCACTC GAATTTGGAG CCGGCCGGCG
  361 TGTGCAAAAG AGGTGAATCG AACGAAAGAC CCGTGTGTAA AGCCGCGTTT CCAAAATGTA
  421 TAAAACCGAG AGCATCTGGC CAATGTGCAT CAGTTGTGGT CAGCAGCAAA ATCAAGTGAA
  481 TCATCTCAGT GCAACTAAAG GGGGGATCCG ATCTCAATAT GAAGTTATGC ATATTACTGG
  541 CCGTCGTGGC CTTTGTTGGC CTCTCGCTCG GGAGATCTTC GCGAACTAGT CCGGANATCC
  601 ACGTGTGTAC NCCTAGGCTT AAGTCTAGAG GGNCCTTCNA ANGNNNNCCT ATCCNNAANC
  661 CTCTNCTCNG TCTCGANTCN ACNNNTANNN GNCNTCNTCA NNNTCNNNNN NNANNTNNAN
721 NCNNNNNNNN NN
//
Primer: reverse
Region: SV40 pA, 6xHis, V5 tag, BiP signal sequence
Sequence file: RDB18945_B1Cue.seq check
>09005_18945_B1Cu_3_reverse_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNCGNNTGNN NNTAATCNGC CNTACNNCAT TTGTAGAGGT TTTACTTGCT
   61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
  121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
  181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
  241 TCTTATCATG TCTGGATCCT CGATCGAGGC TGATCAGCGA GCTCAGGCCT TAGAAGGCAC
  301 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTACGCGT
  361 AGAATCGAGA CCGAGGAGAG GGTTAGGGAT AGGCTTACCT TCGAAGGGCC CTCTAGACTT
  421 AAGCCTAGGT GTACACACGT GGATATCCGG ACTAGTTCGC GAAGATCTCC CGAGCGAGAG
  481 GCCAACAAAG GCCACGACGG CCAGTAATAT GCATAACTTC ATATTGAGAT CGGATCCCCC
  541 CTTTAGTTGC ACTGANATGA NTCACTTGAT TTTGCTGCTG ANCACAACTG ATGCACATTG
  601 NCCAGATGCT CTCGGTTTTA TACNTTTTGN AAACGCGGCT TTACNCACGG GTCTTTCNTT
661 CGANTCNCCT CNTTTGNNCN CNCCNGNNNG CNNNNANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

2021.05.31

GNP_filter3_RDBDEP_html_210503.pl