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pMT-PURO2R

Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells

Catalog number RDB09005
Resource name pMT-PURO2R
Clone info. Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Discosoma sp. red fluorescent protein cDNA
Depositor|Developer Iwaki, Takayuki |
Other clones in our bank

External Database
Discosoma sp. red fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
            Ordering Please visit Information of Request for Distribution.[link] 
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            Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
            Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
            The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
            備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
            本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)をご参照ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09005 pMT-PURO2R DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB09005zzk02.seq provided by the depositor

            Original reference

            original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18945_B1Cup1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: mRFP1-inner1
            Region: DsRed2 3'
            Sequence file: RDB18945_B1Cua.seq check
            >09005_18945_B1Cu_3_mRFP1-inner1_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNCNNN GNNTCCTCCC TGCAGGACGG CTGCTTCNTC TACAAGGTGA
               61 AGTTCATCGG CGTGAACTTC CCCTCCGACG GCCCCGTGAT GCAGAAGAAG ACCATGGGCT
              121 GGGAGGCCTC CACCGAGCGC CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGACCCACA
              181 AGGCCCTGAA GCTGAAGGAC GGCGGCCACT ACCTGGTGGA GTTCAAGTCC ATCTACATGG
              241 CCAAGAAGCC CGTGCAGCTG CCCGGCTACT ACTACGTGGA CGCCAAGCTG GACATCACCT
              301 CCCACAACGA GGACTACACC ATCGTGGAGC AGTACGAGCG CACCGAGGGC CGCCACCACC
              361 TGTTCCTGTA GGAGGCCCAC CGACTCTAGA TCAAGCTTAT CGATACCGTC GACCTCGAGG
              421 CCAGCTAGCT TGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
              481 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
              541 ATCAATGTAT CTTATCATGT CTGGATCGGG TACCGGCGCC TGATGCGGTA TTTTCTCCTT
              601 ACGCATCTGT GCGGNATTTN NCACCGCATA TGGTGNACTC TCNGTACAAT CTGCTCTGAN
              661 GNCGCANANN TAANCNNGCC CCNNNNCCNG CCNNCNCCNN CTGNNNNNNC NNNNNNGGNN
            721 NGNNNNNNNC NNNNNNNNNN
            //
            Primer: PuroR_F
            Region: PuroR, DsRed2 5'
            Sequence file: RDB18945_B1Cub.seq check
            >09005_18945_B1Cu_3_PuroR_F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAAN GANCGCGCAC CTGNGTGCAT GACCCGCAAG
               61 CCCGGTGCCA TGGCCTCCTC CGAGAACGTC ATCACCGAGT TCATGCGCTT CAAGGTGCGC
              121 ATGGAGGGCA CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC
              181 TACGAGGGCC ACAACACCGT GAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC
              241 TGGGACATCC TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC
              301 GACATCCCCG ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG
              361 AACTTCGAGG ACGGCGGCGT GGCGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTGC
              421 TTCATCTACA AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTGATGCAG
              481 AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG
              541 AANGGCGAGA NCCACAAGGC CCTGAAGCTG ANNGACGGCG GNCACTACCT GGTGGAGTTC
              601 NAGTCCATCT ACNTGGCCNA GAANNCCGTG CAGCTGNCCG GCTNCTACTA CNTGGACGCC
              661 NAGCTGGANN TCNCCTCCCN CNACGAGNAN TACNNNTNNN GGNNNANTAC NNNNGCNNNN
            721 ANGNNNNNCN NNNNNNGNNN NNNNNNN
            //
            Primer: COPIApro-F
            Region: copia pro, PuroR
            Sequence file: RDB18945_B1Cuc.seq check
            >09005_18945_B1Cu_3_COPIApro-F_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNCTTTNN NTTCCTTCTT TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG
               61 GTCGTGGTGC TGGTGTTGCA GTTGAAATAA CTTAAAATAT AAATCATAAA ACTCAAACAT
              121 AAACTTGACT ATTTATTTAT TTATTAAGAA AGGAAATATA AATTATAAAT TACAACAGAT
              181 CCCCCGGGCT GCAGGAATTC GATAGCTTGC GCCGCCACCA TGACCGAGTA CAAGCCCACG
              241 GTGCGCCTCG CCACCCGCGA CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC
              301 GCCGACTACC CCGCCACGCG CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC
              361 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG
              421 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG
              481 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA
              541 CAGATGGAAG GNCTCCTGGC GCCGCACCGG CCCAANGAGC CCGCGTGGTT CCTGGCCACC
              601 GTCNGCGTCT CGNCCGACCA CCAGGGCAAG GNNNNNGGNN GCGCCNNCNN GNTCCCCGGA
              661 NNNNANNNGG NCGANCGCGN CNGGGTGNCC GNNNTNNNGN NNNNNTNCNN NNCCNNNNAN
            721 NTCCCNNTNN NNNNNNNN
            //
            Primer: HB-NOS_R
            Region: MT pro, BiP signal sequence
            Sequence file: RDB18945_B1Cud.seq check
            >09005_18945_B1Cu_3_HB-NOS_R_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNTTG GGTANCGCCN GGGTTTTCCC NGTCACGACG TTGTAAAACG
               61 ACGGCCAGTG AATTAATTCG TTGCAGGACA GGATGTGGTG CCCGATGTGA CTAGCTCTTT
              121 GCTGCAGGCC GTCCTATCCT CTGGTTCCGA TAAGAGACCC AGAACTCCGG CCCCCCACCG
              181 CCCACCGCCA CCCCCATACA TATGTGGTAC GCAAGTAAGA GTGCCTGCGC ATGCCCCATG
              241 TGCCCCACCA AGAGCTTTGC ATCCCATACA AGTCCCCAAA GTGGAGAACC GAACCAATTC
              301 TTCGCGGGCA GAACAAAAGC TTCTGCACAC GTCTCCACTC GAATTTGGAG CCGGCCGGCG
              361 TGTGCAAAAG AGGTGAATCG AACGAAAGAC CCGTGTGTAA AGCCGCGTTT CCAAAATGTA
              421 TAAAACCGAG AGCATCTGGC CAATGTGCAT CAGTTGTGGT CAGCAGCAAA ATCAAGTGAA
              481 TCATCTCAGT GCAACTAAAG GGGGGATCCG ATCTCAATAT GAAGTTATGC ATATTACTGG
              541 CCGTCGTGGC CTTTGTTGGC CTCTCGCTCG GGAGATCTTC GCGAACTAGT CCGGANATCC
              601 ACGTGTGTAC NCCTAGGCTT AAGTCTAGAG GGNCCTTCNA ANGNNNNCCT ATCCNNAANC
              661 CTCTNCTCNG TCTCGANTCN ACNNNTANNN GNCNTCNTCA NNNTCNNNNN NNANNTNNAN
            721 NCNNNNNNNN NN
            //
            Primer: reverse
            Region: SV40 pA, 6xHis, V5 tag, BiP signal sequence
            Sequence file: RDB18945_B1Cue.seq check
            >09005_18945_B1Cu_3_reverse_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNCGNNTGNN NNTAATCNGC CNTACNNCAT TTGTAGAGGT TTTACTTGCT
               61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
              121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
              181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
              241 TCTTATCATG TCTGGATCCT CGATCGAGGC TGATCAGCGA GCTCAGGCCT TAGAAGGCAC
              301 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTACGCGT
              361 AGAATCGAGA CCGAGGAGAG GGTTAGGGAT AGGCTTACCT TCGAAGGGCC CTCTAGACTT
              421 AAGCCTAGGT GTACACACGT GGATATCCGG ACTAGTTCGC GAAGATCTCC CGAGCGAGAG
              481 GCCAACAAAG GCCACGACGG CCAGTAATAT GCATAACTTC ATATTGAGAT CGGATCCCCC
              541 CTTTAGTTGC ACTGANATGA NTCACTTGAT TTTGCTGCTG ANCACAACTG ATGCACATTG
              601 NCCAGATGCT CTCGGTTTTA TACNTTTTGN AAACGCGGCT TTACNCACGG GTCTTTCNTT
            661 CGANTCNCCT CNTTTGNNCN CNCCNGNNNG CNNNNANNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl