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pMT-PURO2R (#RDB09005)

Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells

Clone info. Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB + Amp
Gene/insert name Discosoma sp. red fluorescent protein cDNA
Depositor|Developer Iwaki, Takayuki |
 
Sequence (full) RDB09005hts01.seq checkAssembled from experimentally sequenced data.
Publication Nagahashi, K., Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964. [link to RRC of NBRP]
Test sheet RDB09005B0p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09005 pMT-PURO2R DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pMT-PURO2R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09005).

Reference section:

Nagahashi, K., Umemura, K., Kanayama, N., Iwaki, T., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964. [link to RRC of NBRP]