RDB09005 - pMT-PURO2R

Resource data sheet
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pMT-PURO2R

Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells

Catalog number	RDB09005
Resource name	pMT-PURO2R
Clone info.	Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone	pMT-PURO2 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Discosoma sp. red fluorescent protein cDNA
Depositor	Iwaki, Takayuki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).

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Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
Please use the Exclusive MTA (Clontech FPs).

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Exclusive MTA [Link]
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Please use the Exclusive MTA (Clontech FPs).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 専用MTA（英語版のみ）[Link]
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。専用MTA（Clontech FPs, 英語版のみ）をお使いください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB09005	pMT-PURO2R	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB09005z.seq from Depositor

Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB09005_A3I6.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: V5_Reverse	RDB09005_A3I6a.seq
>09005_09005_A3I6_V5_Reverse_B03_06.ab1 1 CATCGAGGCT CTAGACTTAA GCCTAGGTGT ACACACGTGG ATATCCGGAC TAGTTCGCGA 61 AGATCTCCCG AGCGAGAGGC CAACAAAGGC CACGACGGCC AGTAATATGC ATAACTTCAT 121 ATTGAGATCG GATCCCCCCT TTAGTTGCAC TGAGATGATT CACTTGATTT TGCTGCTGAC 181 CACAACTGAT GCACATTGGC CAGATGCTCT CGGTTTTATA CATTTTGGAA ACGCGGCTTT 241 ACACACGGGT CTTTCGTTCG ATTCACCTCT TTTGCACACG CCGGCCGGCT CCAAATTCGA 301 GTGGAGACGT GTGCAGAAGC TTTTGTTCTG CCCGCGAAGA ATTGGTTCGG TTCTCCACTT 361 TGGGGACTTG TATGGGATGC AAAGCTCTTG GTGGGGCACA TGGGGCATGC GCAGGCACTC 421 TTACTTGCGT ACCACATATG TATGGGGGTG GCGGTGGGCG GTGGGGGGCC GGAGTTCTGG 481 GTCTCTTATC GGAACCAGAG GATAGGACGG CCTGCAGCAA AGAGCTAGTC ACATCGGGCA 541 CCACATCCTG TCCTGCAACG AATTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG 601 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG 661 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 721 GAATGGCGCT AGAGGATCCG GTGCGTGGTG GTTTCATGCT TCTGGGAACG GCAAATGGGT 781 TTAGGATTGG GAACCCCTCA TCATCTGTTG GAATATACTA TTCAACCTAC AAAAGTAACG 841 TTAAACAACA CTACTTTATA TTTGATATGA ATGGCCACAC CTTTTATGCC ATAAAACATA 901 TTGTAAGAGA ATACCACTCT TTTTATTCCT TCTTTCCTTC TTGTACGTTT TTTGCTGTAA 961 GTAGGTCGTG GTGCTGGTGT TGCAGTTGAA TAACTTAAAA TATAAATCAT AAAACTCAAC 1021 ATAAACTTTG ACTATTTATT TATTTATTAA GAAGGAAATA TAATTATAAT TTACACAGAT 1081 CCCCCGGCTG CAGGAATTCG AATAGCTGCG CGCCACATGA ACGAGTACAG GCCCACGATG 1141 CGCCTCGGTC CAACCCGCCG GAACCGAACG T //
Primer: Amp_R	RDB09005_A3I6b.seq
>09005_09005_A3I6_Amp_R_A03_03.ab1 1 CTTGTTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 181 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC 241 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG 301 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA 361 TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT 421 ACCGCATCAG GCGCCGGTAC CCGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA 481 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC 541 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTCAA GCTAGCTGGC CTCGAGGTCG 601 ACGGTATCGA TAAGCTTGAT CTAGAGTCGG TGGGCCTCCT ACAGGAACAG GTGGTGGCGG 661 CCCTCGGTGC GCTCGTACTG CTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC 721 AGCTTGGCGT CCACGTAGTA GTAGCCGGGC AGCTGCACGG GCTTCTTGGC CATGTAGATG 781 GACTTGAACT CCACCAGGTA GTGGCCGCCG TCCTTCAGCT TCAGGGCCTT GTGGGTCTCG 841 CCCTTCAGCA CGCCGTCGCG GGGGTACAGG CGCTCGGTGG AGGCCTCCCA GCCCATGGTC 901 TTCTTCTGCA TCACGGGGCC GTCGGAGGGG AAGTTCACGC CGATGAACTT CACCTTGTAG 961 ATGAAGCAGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG TCGCCACGCC GCCGTCCTCG 1021 AAGTTCATCA CGCGCTCCAC TTGAAGCCCT CGGGGAGACA GCTTCTTGTA GTCGGGATGT 1081 CGCGGGTGCT TTCACGTACA CAGCAGCGTA CTGGAACTGG GGGGAACAGG GATGTTCCCA 1141 AGGGCGTAAG GTCCAG //

Please visit Sequencing and PCR primers for primer information.

References

original	Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

2018.12.11

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