Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMT-PURO2R

Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18945_B1Cup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mRFP1-inner1 Region: DsRed2 3' Sequence file: RDB18945_B1Cua.seq
>09005_18945_B1Cu_3_mRFP1-inner1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNCNNN GNNTCCTCCC TGCAGGACGG CTGCTTCNTC TACAAGGTGA    61 AGTTCATCGG CGTGAACTTC CCCTCCGACG GCCCCGTGAT GCAGAAGAAG ACCATGGGCT   121 GGGAGGCCTC CACCGAGCGC CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGACCCACA   181 AGGCCCTGAA GCTGAAGGAC GGCGGCCACT ACCTGGTGGA GTTCAAGTCC ATCTACATGG   241 CCAAGAAGCC CGTGCAGCTG CCCGGCTACT ACTACGTGGA CGCCAAGCTG GACATCACCT   301 CCCACAACGA GGACTACACC ATCGTGGAGC AGTACGAGCG CACCGAGGGC CGCCACCACC   361 TGTTCCTGTA GGAGGCCCAC CGACTCTAGA TCAAGCTTAT CGATACCGTC GACCTCGAGG   421 CCAGCTAGCT TGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC   481 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC   541 ATCAATGTAT CTTATCATGT CTGGATCGGG TACCGGCGCC TGATGCGGTA TTTTCTCCTT   601 ACGCATCTGT GCGGNATTTN NCACCGCATA TGGTGNACTC TCNGTACAAT CTGCTCTGAN   661 GNCGCANANN TAANCNNGCC CCNNNNCCNG CCNNCNCCNN CTGNNNNNNC NNNNNNGGNN 721 NGNNNNNNNC NNNNNNNNNN //
Primer: PuroR_F Region: PuroR, DsRed2 5' Sequence file: RDB18945_B1Cub.seq
>09005_18945_B1Cu_3_PuroR_F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAAN GANCGCGCAC CTGNGTGCAT GACCCGCAAG    61 CCCGGTGCCA TGGCCTCCTC CGAGAACGTC ATCACCGAGT TCATGCGCTT CAAGGTGCGC   121 ATGGAGGGCA CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC   181 TACGAGGGCC ACAACACCGT GAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC   241 TGGGACATCC TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC   301 GACATCCCCG ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG   361 AACTTCGAGG ACGGCGGCGT GGCGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTGC   421 TTCATCTACA AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTGATGCAG   481 AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG   541 AANGGCGAGA NCCACAAGGC CCTGAAGCTG ANNGACGGCG GNCACTACCT GGTGGAGTTC   601 NAGTCCATCT ACNTGGCCNA GAANNCCGTG CAGCTGNCCG GCTNCTACTA CNTGGACGCC   661 NAGCTGGANN TCNCCTCCCN CNACGAGNAN TACNNNTNNN GGNNNANTAC NNNNGCNNNN 721 ANGNNNNNCN NNNNNNGNNN NNNNNNN //
Primer: COPIApro-F Region: copia pro, PuroR Sequence file: RDB18945_B1Cuc.seq
>09005_18945_B1Cu_3_COPIApro-F_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNCTTTNN NTTCCTTCTT TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG    61 GTCGTGGTGC TGGTGTTGCA GTTGAAATAA CTTAAAATAT AAATCATAAA ACTCAAACAT   121 AAACTTGACT ATTTATTTAT TTATTAAGAA AGGAAATATA AATTATAAAT TACAACAGAT   181 CCCCCGGGCT GCAGGAATTC GATAGCTTGC GCCGCCACCA TGACCGAGTA CAAGCCCACG   241 GTGCGCCTCG CCACCCGCGA CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC   301 GCCGACTACC CCGCCACGCG CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC   361 GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG   421 GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG   481 TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA   541 CAGATGGAAG GNCTCCTGGC GCCGCACCGG CCCAANGAGC CCGCGTGGTT CCTGGCCACC   601 GTCNGCGTCT CGNCCGACCA CCAGGGCAAG GNNNNNGGNN GCGCCNNCNN GNTCCCCGGA   661 NNNNANNNGG NCGANCGCGN CNGGGTGNCC GNNNTNNNGN NNNNNTNCNN NNCCNNNNAN 721 NTCCCNNTNN NNNNNNNN //
Primer: HB-NOS_R Region: MT pro, BiP signal sequence Sequence file: RDB18945_B1Cud.seq
>09005_18945_B1Cu_3_HB-NOS_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTTG GGTANCGCCN GGGTTTTCCC NGTCACGACG TTGTAAAACG    61 ACGGCCAGTG AATTAATTCG TTGCAGGACA GGATGTGGTG CCCGATGTGA CTAGCTCTTT   121 GCTGCAGGCC GTCCTATCCT CTGGTTCCGA TAAGAGACCC AGAACTCCGG CCCCCCACCG   181 CCCACCGCCA CCCCCATACA TATGTGGTAC GCAAGTAAGA GTGCCTGCGC ATGCCCCATG   241 TGCCCCACCA AGAGCTTTGC ATCCCATACA AGTCCCCAAA GTGGAGAACC GAACCAATTC   301 TTCGCGGGCA GAACAAAAGC TTCTGCACAC GTCTCCACTC GAATTTGGAG CCGGCCGGCG   361 TGTGCAAAAG AGGTGAATCG AACGAAAGAC CCGTGTGTAA AGCCGCGTTT CCAAAATGTA   421 TAAAACCGAG AGCATCTGGC CAATGTGCAT CAGTTGTGGT CAGCAGCAAA ATCAAGTGAA   481 TCATCTCAGT GCAACTAAAG GGGGGATCCG ATCTCAATAT GAAGTTATGC ATATTACTGG   541 CCGTCGTGGC CTTTGTTGGC CTCTCGCTCG GGAGATCTTC GCGAACTAGT CCGGANATCC   601 ACGTGTGTAC NCCTAGGCTT AAGTCTAGAG GGNCCTTCNA ANGNNNNCCT ATCCNNAANC   661 CTCTNCTCNG TCTCGANTCN ACNNNTANNN GNCNTCNTCA NNNTCNNNNN NNANNTNNAN 721 NCNNNNNNNN NN //
Primer: reverse Region: SV40 pA, 6xHis, V5 tag, BiP signal sequence Sequence file: RDB18945_B1Cue.seq
>09005_18945_B1Cu_3_reverse_G01_07_ABI08.ab1     1 NNNNNNNNNN NNCGNNTGNN NNTAATCNGC CNTACNNCAT TTGTAGAGGT TTTACTTGCT    61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT   121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC   181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA   241 TCTTATCATG TCTGGATCCT CGATCGAGGC TGATCAGCGA GCTCAGGCCT TAGAAGGCAC   301 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTACGCGT   361 AGAATCGAGA CCGAGGAGAG GGTTAGGGAT AGGCTTACCT TCGAAGGGCC CTCTAGACTT   421 AAGCCTAGGT GTACACACGT GGATATCCGG ACTAGTTCGC GAAGATCTCC CGAGCGAGAG   481 GCCAACAAAG GCCACGACGG CCAGTAATAT GCATAACTTC ATATTGAGAT CGGATCCCCC   541 CTTTAGTTGC ACTGANATGA NTCACTTGAT TTTGCTGCTG ANCACAACTG ATGCACATTG   601 NCCAGATGCT CTCGGTTTTA TACNTTTTGN AAACGCGGCT TTACNCACGG GTCTTTCNTT 661 CGANTCNCCT CNTTTGNNCN CNCCNGNNNG CNNNNANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl