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Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells
| Catalog number | RDB09004 |
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| Resource name | pMT-PURO2G |
| Clone info. | Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable. |
| Vector backbone | pMT-PURO2 (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Aequorea victoria green fluorescent protein cDNA |
| Depositor|Developer | Iwaki, Takayuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)). |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB09004 | pMT-PURO2G | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Sequence RDB09004z.seq |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0056j (Japanese text) |
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| Featured content | Dnaconda's recommendation EXR0056e (English text) |
| Featured content | Empty Backbone (English text) |
| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB09004_A3Gh.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: V5_Reverse Sequence file: RDB09004_A3Gha.seq
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>09004_09004_A3Gh_V5_Reverse_C11_08.ab1 1 CTGGAGGTCT CTAGACTTAA GCCTAGGTGT ACACACGTGG ATATCCGGAC TAGTTCGCGA 61 AGATCTCCCG AGCGAGAGGC CAACAAAGGC CACGACGGCC AGTAATATGC ATAACTTCAT 121 ATTGAGATCG GATCCCCCCT TTAGTTGCAC TGAGATGATT CACTTGATTT TGCTGCTGAC 181 CACAACTGAT GCACATTGGC CAGATGCTCT CGGTTTTATA CATTTTGGAA ACGCGGCTTT 241 ACACACGGGT CTTTCGTTCG ATTCACCTCT TTTGCACACG CCGGCCGGCT CCAAATTCGA 301 GTGGAGACGT GTGCAGAAGC TTTTGTTCTG CCCGCGAAGA ATTGGTTCGG TTCTCCACTT 361 TGGGGACTTG TATGGGATGC AAAGCTCTTG GTGGGGCACA TGGGGCATGC GCAGGCACTC 421 TTACTTGCGT ACCACATATG TATGGGGGTG GCGGTGGGCG GTGGGGGGCC GGAGTTCTGG 481 GTCTCTTATC GGAACCAGAG GATAGGACGG CCTGCAGCAA AGAGCTAGTC ACATCGGGCA 541 CCACATCCTG TCCTGCAACG AATTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG 601 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG 661 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 721 GAATGGCGCT AGAGGATCCG GTGCGTGGTG GTTTCATGCT TCTGGGAACG GCAAATGGGT 781 TTAGGATTGG GAACCCCTCA TCATCTGTTG GAATATACTA TTCAACCTAC AAAAGTAACG 841 TTAAACAACA CTACTTTATA TTTGATATGA ATGGCCACAC CTTTTATGCC ATAAAACATA 901 TTGTAAGAGA ATACCACTCT TTTTATTCCT TCTTTCCTTC TTGTACGTTT TTTTGCTGTA 961 AGTAGGTCGT GGTGCTGGTG TTGCAGTTGA AATAACTTAA AATATAAATC ATAAAACTCA 1021 AACATAAACT TGACTATTTA TTTTATTTAT TAAGAAAGGA AAATATAAAT TAATAAATTA 1081 CACAGGATCC CCCCGGGCTG CAGCATCGAT AGCTGCGCCG GCCACCAATG GACCGAGTAC 1141 AAGCCCCCAC CGGATGGC // |
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Primer: EGFP-C_rev Sequence file: RDB09004_A3Ghb.seq
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>09004_09004_A3Gh_EGFP-C_rev_D11_11.ab1 1 ACCCTCCGAT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA 61 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC 121 TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT 181 CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT 241 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT 301 CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT 361 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG 421 GGTAGCGGCT GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA 481 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC 541 CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA 601 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGCA CCGGGCTTGC 661 GGGTCATGCA CCAGGTGCGC GGTCCTTCGG GCACCTCGAC GTCGGCGGTG ACGGTGAAGC 721 CGAGCCGCTC GTAGAAGGGG AGGTTGCGGG GCGCGGAGGT CTCCAGGAAG GCGGGCACCC 781 CGGCGCGCTC GGCCGCCTCC ACTCCGGGGA GCACGACGGC GCTGCCCAGA CCCTTGCCCT 841 GGTGGTCGGG CGAGACGCCG ACGGTGGCCA GGAACCACGC GGGCTCCTTG GGCCGGTGCG 901 GCGCCAGGAG GCCTTCCATC TGTTGCTGCG CGGCCAGCCG GGAACCGCTC AACTCGGCCA 961 TGCGCGGGCC GATCTCGGCG AACACCGCCC CCGCTTCGAC GCTCTCCGGC GTGGTCCAGA 1021 CCGCCCACCG CGGGCGCCCG TCGTCCGCGA CCCACACCCT TTGCCCGAAT GGTCGAGCCC 1081 GACGCGCGTG AGGAGGAGTC CTGCCAGCTC GGCTGACCCC GGCCTCGGAT GTGCGGATTC 1141 CGGGATTCCG ACG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964. |
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| reference | Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807. |
2020.12.14
GNP_filter3_RDBDEP_html_201104.pl