Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMT-PURO2G

Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB09004_A3Cjp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F Region: EGFP 3',SV40 pA Sequence file: RDB09004_A3Cja.seq
>09004_09004_B1C4F_mVenus-M_F_20_C03_09_ABI24.ab1     1 NNNNNNNNNN NGGNATNNAG GTGNACTTNN AGATCCGCCA CAACATCGAG GACGGCAGCG    61 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC   121 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG   181 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC   241 TGTACAAGTA AGAGGCCCAC CGACTCTAGA TCAAGCTTAT CGATACCGTC GACCTCGAGG   301 CCAGCTAGCT TGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC   361 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC   421 ATCAATGTAT CTTATCATGT CTGGATCGGG TACCGGCGCC TGATGCGGTA TTTTCTCCTT   481 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT   541 GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGANGCGCC CTGACGGGCT   601 TGTCTGNTCC CGGCATCCGC TNACNGACNA GCTGTGANCG TCTCCNGGNN CTGNNTGNGT 661 CNNANGNTTN CNNNNTNNTC NCCGAAACGC NCGANNNNAA NNNNNNNNNN //
Primer: EGFP-C-rev Region: EGFP 5',PuroR Sequence file: RDB09004_A3Cjb.seq
>9004_EGFP-C-rev_F01_16.ab1     1 NNNNNNNNNN NNNNNNTNGC NNNGGGCGGN NTGGGTGCTC NGGTAGTGGT TGTCGGGCAG    61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT   121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC   181 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT   241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC   301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC   361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG   421 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC   481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC   541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG   601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGCAC CGGGCTTGCG   661 GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG TCGGCGGTGA CGGTGAAGCC   721 GAGCCGCTCG TANAAGGGGA GGTTGCGGGG CGCGGANGTC TCCAGGAAGG CGGGCACCCC   781 GGCGCGCTCG GCCGCCTCCA CTCCGGGGAN CACGACGGCG CTGCCCAGAC CCTTGCCCTN   841 GTGGTCGGGC GANANGCCGA CGGTGGCCAG GAACCACGCG GGCTCCTTGN GCCGGNGCGG   901 CGCCAGGNNG NCTTNCATCT GNTGCTGCGC GGCCAGCCNG GAACCGCTCA ACTCNGCCAT 961 GCGCGGGCCN NTCTCGGCGA ANNCCGCCCC CGCTTCNNNG CNNNCCNNNN NNNNNN //
Primer: COPIApro-F Region: copia pro,PuroR Sequence file: RDB09004_A3Cjc.seq
>09004_09004_B1C4F_COPIApro_F_10_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CTTTTNNTTC CTTCTTTCCT TCTTGTACGT TTTTTGCTGT    61 AAGTAGGTCG TGGTGCTGGT GTTGCAGTTG AAATAACTTA AAATATAAAT CATAAAACTC   121 AAACATAAAC TTGACTATTT ATTTATTTAT TAAGAAAGGA AATATAAATT ATAAATTACA   181 ACAGATCCCC CGGGCTGCAG GAATTCGATA GCTTGCGCCG CCACCATGAC CGAGTACAAG   241 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC   301 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG   361 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG   421 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG   481 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG   541 CAGCAACAGA TGGAAGGNCT CCTGGCGCCG CACCGGCCCN AGGAGCCCGC GTGGTTCCTG   601 GCCNCNGTCN GCGTCTCGCC CGANCNCCNN GGCAAGGGTC TGGGCAGCGC CGTCNNGCTC   661 CCCGNANNNN NNGCNGNNNA NCGNNNNGGN NNNCNNCNTT NCTNNNNANN TNCGNNCCCN 721 CNNCNNCCNN NNNNNNNN //
Primer: HB-NUS_R Region: MT pro,Bip signal seq,V5 tag,6XHis Sequence file: RDB09004_A3Cjd.seq
>09004_09004_B1C4F_HB-NOS_R_G02_20_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNNT GGGTNNNGCC NGGGTTTTCC CNGTCACGAC GTTGTAAAAC    61 GACGGCCAGT GAATTAATTC GTTGCAGGAC AGGNTGTGGT GCCCGATGTG ACTAGCTCTT   121 TGCTGCAGGC CGTCCTATCC TCTGGTTCCG ATAAGAGACC CAGAACTCCG GCCCCCCACC   181 GCCCACCGCC ACCCCCATAC ATATGTGGTA CGCAAGTAAG AGTGCCTGCG CATGCCCCAT   241 GTGCCCCACC AAGAGCTTTG CATCCCATAC AAGTCCCCAA AGTGGAGAAC CGAACCAATT   301 CTTCGCGGGC AGAACAAAAG CTTCTGCACA CGTCTCCACT CGAATTTGGA GCCGGCCGGC   361 GTGTGCAAAA GAGGTGAATC GAACGAAAGA CCCGTGTGTA AAGCCGCGTT TCCAAAATGT   421 ATAAAACCGA GAGCATCTGG CCAATGTGCA TCAGTTGTGG TCAGCAGCAA AATCAAGTGA   481 ATCATCTCAG TGCAACTAAA GGGGGGATCC GATCTCAATA TGAAGTTATG CATATTACTG   541 GCCGTCGTGG CCTTTGTTGG CCTCTCGCTC GGGAGATCTT CGCGAACTAG TCCGGATATC   601 CACGTGTGTA CACCTAGGCT TAAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC   661 CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA   721 ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AANGCCTGAN CTCGCTGATC AGCCTCGATC   781 GAGGANCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG   841 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GANGCTATTG CTTTATTTGT AACCATTATA   901 AGCTGCAATA AACAAGTTAA CAACNACNAT NGCNTTNNTT TTANGTTTCN NNNTCNGGGG   961 GANGTGNGGG ANNNTTTTTN NAAGNANGTA AAANNNNNAN NAATGNGGNA NGGCTGANNA 1021 NGNTCANNNN NNCNNNNNNN NNN //
Primer: V5 Reverse Region: Bip signal seq,MT pro Sequence file: RDB09004_A3Cje.seq
>09004_09004_B1C4F_V5 Reverse_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNTTNNNCC TAGGTGTNNN CNCGTGGNNA TCCGGACTAG TTCGCGAAGA    61 NCTCCCGAGC GAGAGGCCAA CAAAGGCCAC GACGGCCAGT AATATGCATA ACTTCATATT   121 GAGATCGGAT CCCCCCTTTA GTTGCACTGA GATGATTCAC TTGATTTTGC TGCTGACCAC   181 AACTGATGCA CATTGGCCAG ATGCTCTCGG TTTTATACAT TTTGGAAACG CGGCTTTACA   241 CACGGGTCTT TCGTTCGATT CACCTCTTTT GCACACGCCG GCCGGCTCCA AATTCGAGTG   301 GAGACGTGTG CAGAAGCTTT TGTTCTGCCC GCGAAGAATT GGTTCGGTTC TCCACTTTGG   361 GGACTTGTAT GGGATGCAAA GCTCTTGGTG GGGCACATGG GGCATGCGCA GGCACTCTTA   421 CTTGCGTACC ACATATGTAT GGGGGTGGCG GTGGGCGGTG GGGGGCCGGA GTTCTGGGTC   481 TCTTATCGGA ACCAGAGGAT AGGACGGCCT GCAGCAAAGA GCTAGTCACA TCGGGCACCA   541 CATCCTGTCC TGCAACGAAT TAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA   601 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT   661 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA   721 TGGCGCTAGA GGATCCGGTG CGTGGTGGTT TCATGCTTCT GGGAACGGCA AATGGGTTTA   781 GGANTGGGAA CNNCTCATCA TCTGTTGGAA TATACTATTC AACCTACAAA AGTAACGTTA   841 AACAACACTA CTTTATATTT GATATGAATG GNCACACCTT TTATGNNNTA AAACATATTG   901 TAAGANAATA CCACTCTTTT TATNCCTTCT TTCCNNCNNG TANNTTTTTT TGCTGTNNNT   961 NNNTCNNGNN NNTGGNGNNG CAGTTGAAAT ANNNTAAAAN ATAAATCNNN AAANNNNNNN 1021 NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl