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pMT-PURO2G

Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells

Catalog number RDB09004
Resource name pMT-PURO2G
Clone info. Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor|Developer Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
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Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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RDB09004 pMT-PURO2G DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB09004zzk04.seq

Original reference

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0056j (Japanese text)
Featured content Dnaconda's recommendation EXR0056e (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09004_A3Cjp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F
Region: EGFP 3',SV40 pA
Sequence file: RDB09004_A3Cja.seq check
>09004_09004_B1C4F_mVenus-M_F_20_C03_09_ABI24.ab1
    1 NNNNNNNNNN NGGNATNNAG GTGNACTTNN AGATCCGCCA CAACATCGAG GACGGCAGCG
   61 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
  121 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG
  181 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC
  241 TGTACAAGTA AGAGGCCCAC CGACTCTAGA TCAAGCTTAT CGATACCGTC GACCTCGAGG
  301 CCAGCTAGCT TGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
  361 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
  421 ATCAATGTAT CTTATCATGT CTGGATCGGG TACCGGCGCC TGATGCGGTA TTTTCTCCTT
  481 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT
  541 GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGANGCGCC CTGACGGGCT
  601 TGTCTGNTCC CGGCATCCGC TNACNGACNA GCTGTGANCG TCTCCNGGNN CTGNNTGNGT
661 CNNANGNTTN CNNNNTNNTC NCCGAAACGC NCGANNNNAA NNNNNNNNNN
//
Primer: EGFP-C-rev
Region: EGFP 5',PuroR
Sequence file: RDB09004_A3Cjb.seq check
>9004_EGFP-C-rev_F01_16.ab1
    1 NNNNNNNNNN NNNNNNTNGC NNNGGGCGGN NTGGGTGCTC NGGTAGTGGT TGTCGGGCAG
   61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
  121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
  181 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  421 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
  541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGCAC CGGGCTTGCG
  661 GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG TCGGCGGTGA CGGTGAAGCC
  721 GAGCCGCTCG TANAAGGGGA GGTTGCGGGG CGCGGANGTC TCCAGGAAGG CGGGCACCCC
  781 GGCGCGCTCG GCCGCCTCCA CTCCGGGGAN CACGACGGCG CTGCCCAGAC CCTTGCCCTN
  841 GTGGTCGGGC GANANGCCGA CGGTGGCCAG GAACCACGCG GGCTCCTTGN GCCGGNGCGG
  901 CGCCAGGNNG NCTTNCATCT GNTGCTGCGC GGCCAGCCNG GAACCGCTCA ACTCNGCCAT
961 GCGCGGGCCN NTCTCGGCGA ANNCCGCCCC CGCTTCNNNG CNNNCCNNNN NNNNNN
//
Primer: COPIApro-F
Region: copia pro,PuroR
Sequence file: RDB09004_A3Cjc.seq check
>09004_09004_B1C4F_COPIApro_F_10_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CTTTTNNTTC CTTCTTTCCT TCTTGTACGT TTTTTGCTGT
   61 AAGTAGGTCG TGGTGCTGGT GTTGCAGTTG AAATAACTTA AAATATAAAT CATAAAACTC
  121 AAACATAAAC TTGACTATTT ATTTATTTAT TAAGAAAGGA AATATAAATT ATAAATTACA
  181 ACAGATCCCC CGGGCTGCAG GAATTCGATA GCTTGCGCCG CCACCATGAC CGAGTACAAG
  241 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC
  301 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG
  361 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
  421 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
  481 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
  541 CAGCAACAGA TGGAAGGNCT CCTGGCGCCG CACCGGCCCN AGGAGCCCGC GTGGTTCCTG
  601 GCCNCNGTCN GCGTCTCGCC CGANCNCCNN GGCAAGGGTC TGGGCAGCGC CGTCNNGCTC
  661 CCCGNANNNN NNGCNGNNNA NCGNNNNGGN NNNCNNCNTT NCTNNNNANN TNCGNNCCCN
721 CNNCNNCCNN NNNNNNNN
//
Primer: HB-NUS_R
Region: MT pro,Bip signal seq,V5 tag,6XHis
Sequence file: RDB09004_A3Cjd.seq check
>09004_09004_B1C4F_HB-NOS_R_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNT GGGTNNNGCC NGGGTTTTCC CNGTCACGAC GTTGTAAAAC
   61 GACGGCCAGT GAATTAATTC GTTGCAGGAC AGGNTGTGGT GCCCGATGTG ACTAGCTCTT
  121 TGCTGCAGGC CGTCCTATCC TCTGGTTCCG ATAAGAGACC CAGAACTCCG GCCCCCCACC
  181 GCCCACCGCC ACCCCCATAC ATATGTGGTA CGCAAGTAAG AGTGCCTGCG CATGCCCCAT
  241 GTGCCCCACC AAGAGCTTTG CATCCCATAC AAGTCCCCAA AGTGGAGAAC CGAACCAATT
  301 CTTCGCGGGC AGAACAAAAG CTTCTGCACA CGTCTCCACT CGAATTTGGA GCCGGCCGGC
  361 GTGTGCAAAA GAGGTGAATC GAACGAAAGA CCCGTGTGTA AAGCCGCGTT TCCAAAATGT
  421 ATAAAACCGA GAGCATCTGG CCAATGTGCA TCAGTTGTGG TCAGCAGCAA AATCAAGTGA
  481 ATCATCTCAG TGCAACTAAA GGGGGGATCC GATCTCAATA TGAAGTTATG CATATTACTG
  541 GCCGTCGTGG CCTTTGTTGG CCTCTCGCTC GGGAGATCTT CGCGAACTAG TCCGGATATC
  601 CACGTGTGTA CACCTAGGCT TAAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC
  661 CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA
  721 ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AANGCCTGAN CTCGCTGATC AGCCTCGATC
  781 GAGGANCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
  841 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GANGCTATTG CTTTATTTGT AACCATTATA
  901 AGCTGCAATA AACAAGTTAA CAACNACNAT NGCNTTNNTT TTANGTTTCN NNNTCNGGGG
  961 GANGTGNGGG ANNNTTTTTN NAAGNANGTA AAANNNNNAN NAATGNGGNA NGGCTGANNA
1021 NGNTCANNNN NNCNNNNNNN NNN
//
Primer: V5 Reverse
Region: Bip signal seq,MT pro
Sequence file: RDB09004_A3Cje.seq check
>09004_09004_B1C4F_V5 Reverse_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNTTNNNCC TAGGTGTNNN CNCGTGGNNA TCCGGACTAG TTCGCGAAGA
   61 NCTCCCGAGC GAGAGGCCAA CAAAGGCCAC GACGGCCAGT AATATGCATA ACTTCATATT
  121 GAGATCGGAT CCCCCCTTTA GTTGCACTGA GATGATTCAC TTGATTTTGC TGCTGACCAC
  181 AACTGATGCA CATTGGCCAG ATGCTCTCGG TTTTATACAT TTTGGAAACG CGGCTTTACA
  241 CACGGGTCTT TCGTTCGATT CACCTCTTTT GCACACGCCG GCCGGCTCCA AATTCGAGTG
  301 GAGACGTGTG CAGAAGCTTT TGTTCTGCCC GCGAAGAATT GGTTCGGTTC TCCACTTTGG
  361 GGACTTGTAT GGGATGCAAA GCTCTTGGTG GGGCACATGG GGCATGCGCA GGCACTCTTA
  421 CTTGCGTACC ACATATGTAT GGGGGTGGCG GTGGGCGGTG GGGGGCCGGA GTTCTGGGTC
  481 TCTTATCGGA ACCAGAGGAT AGGACGGCCT GCAGCAAAGA GCTAGTCACA TCGGGCACCA
  541 CATCCTGTCC TGCAACGAAT TAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
  601 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
  661 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
  721 TGGCGCTAGA GGATCCGGTG CGTGGTGGTT TCATGCTTCT GGGAACGGCA AATGGGTTTA
  781 GGANTGGGAA CNNCTCATCA TCTGTTGGAA TATACTATTC AACCTACAAA AGTAACGTTA
  841 AACAACACTA CTTTATATTT GATATGAATG GNCACACCTT TTATGNNNTA AAACATATTG
  901 TAAGANAATA CCACTCTTTT TATNCCTTCT TTCCNNCNNG TANNTTTTTT TGCTGTNNNT
  961 NNNTCNNGNN NNTGGNGNNG CAGTTGAAAT ANNNTAAAAN ATAAATCNNN AAANNNNNNN
1021 NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.
reference Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807.

2021.03.23

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