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pMT-PURO2G

Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells

Catalog number RDB09004
Resource name pMT-PURO2G
Clone info. Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09004 pMT-PURO2G DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB09004z.seq from Depositor

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References

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB09004_A3Gh.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: V5_Reverse RDB09004_A3Gha.seq
>09004_09004_A3Gh_V5_Reverse_C11_08.ab1
    1 CTGGAGGTCT CTAGACTTAA GCCTAGGTGT ACACACGTGG ATATCCGGAC TAGTTCGCGA
   61 AGATCTCCCG AGCGAGAGGC CAACAAAGGC CACGACGGCC AGTAATATGC ATAACTTCAT
  121 ATTGAGATCG GATCCCCCCT TTAGTTGCAC TGAGATGATT CACTTGATTT TGCTGCTGAC
  181 CACAACTGAT GCACATTGGC CAGATGCTCT CGGTTTTATA CATTTTGGAA ACGCGGCTTT
  241 ACACACGGGT CTTTCGTTCG ATTCACCTCT TTTGCACACG CCGGCCGGCT CCAAATTCGA
  301 GTGGAGACGT GTGCAGAAGC TTTTGTTCTG CCCGCGAAGA ATTGGTTCGG TTCTCCACTT
  361 TGGGGACTTG TATGGGATGC AAAGCTCTTG GTGGGGCACA TGGGGCATGC GCAGGCACTC
  421 TTACTTGCGT ACCACATATG TATGGGGGTG GCGGTGGGCG GTGGGGGGCC GGAGTTCTGG
  481 GTCTCTTATC GGAACCAGAG GATAGGACGG CCTGCAGCAA AGAGCTAGTC ACATCGGGCA
  541 CCACATCCTG TCCTGCAACG AATTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG
  601 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG
  661 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
  721 GAATGGCGCT AGAGGATCCG GTGCGTGGTG GTTTCATGCT TCTGGGAACG GCAAATGGGT
  781 TTAGGATTGG GAACCCCTCA TCATCTGTTG GAATATACTA TTCAACCTAC AAAAGTAACG
  841 TTAAACAACA CTACTTTATA TTTGATATGA ATGGCCACAC CTTTTATGCC ATAAAACATA
  901 TTGTAAGAGA ATACCACTCT TTTTATTCCT TCTTTCCTTC TTGTACGTTT TTTTGCTGTA
  961 AGTAGGTCGT GGTGCTGGTG TTGCAGTTGA AATAACTTAA AATATAAATC ATAAAACTCA
 1021 AACATAAACT TGACTATTTA TTTTATTTAT TAAGAAAGGA AAATATAAAT TAATAAATTA
 1081 CACAGGATCC CCCCGGGCTG CAGCATCGAT AGCTGCGCCG GCCACCAATG GACCGAGTAC
1141 AAGCCCCCAC CGGATGGC
//
Primer: EGFP-C_rev RDB09004_A3Ghb.seq
>09004_09004_A3Gh_EGFP-C_rev_D11_11.ab1
    1 ACCCTCCGAT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA
   61 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC
  121 TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT
  181 CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT
  241 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT
  301 CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT
  361 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG
  421 GGTAGCGGCT GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA
  481 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC
  541 CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA
  601 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGCA CCGGGCTTGC
  661 GGGTCATGCA CCAGGTGCGC GGTCCTTCGG GCACCTCGAC GTCGGCGGTG ACGGTGAAGC
  721 CGAGCCGCTC GTAGAAGGGG AGGTTGCGGG GCGCGGAGGT CTCCAGGAAG GCGGGCACCC
  781 CGGCGCGCTC GGCCGCCTCC ACTCCGGGGA GCACGACGGC GCTGCCCAGA CCCTTGCCCT
  841 GGTGGTCGGG CGAGACGCCG ACGGTGGCCA GGAACCACGC GGGCTCCTTG GGCCGGTGCG
  901 GCGCCAGGAG GCCTTCCATC TGTTGCTGCG CGGCCAGCCG GGAACCGCTC AACTCGGCCA
  961 TGCGCGGGCC GATCTCGGCG AACACCGCCC CCGCTTCGAC GCTCTCCGGC GTGGTCCAGA
 1021 CCGCCCACCG CGGGCGCCCG TCGTCCGCGA CCCACACCCT TTGCCCGAAT GGTCGAGCCC
 1081 GACGCGCGTG AGGAGGAGTC CTGCCAGCTC GGCTGACCCC GGCCTCGGAT GTGCGGATTC
1141 CGGGATTCCG ACG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

2018.12.11

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