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pMT-PURO2G

Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells

Catalog number RDB09004
Resource name pMT-PURO2G
Clone info. Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Aequorea victoria green fluorescent protein cDNA
Depositor|Developer Iwaki, Takayuki |
Other clones in our bank

External Database
Aequorea victoria green fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology, 65(2), 173-178 (2013)).
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 65(2), 173-178 (2013))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB09004 pMT-PURO2G DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB09004zzk04.seq provided by the depositor

            Original reference

            original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09004_A3Cjp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: mVenus-M_F
            Region: EGFP 3',SV40 pA
            Sequence file: RDB09004_A3Cja.seq check
            >09004_09004_B1C4F_mVenus-M_F_20_C03_09_ABI24.ab1
                1 NNNNNNNNNN NGGNATNNAG GTGNACTTNN AGATCCGCCA CAACATCGAG GACGGCAGCG
               61 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
              121 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG
              181 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC
              241 TGTACAAGTA AGAGGCCCAC CGACTCTAGA TCAAGCTTAT CGATACCGTC GACCTCGAGG
              301 CCAGCTAGCT TGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
              361 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
              421 ATCAATGTAT CTTATCATGT CTGGATCGGG TACCGGCGCC TGATGCGGTA TTTTCTCCTT
              481 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT
              541 GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGANGCGCC CTGACGGGCT
              601 TGTCTGNTCC CGGCATCCGC TNACNGACNA GCTGTGANCG TCTCCNGGNN CTGNNTGNGT
            661 CNNANGNTTN CNNNNTNNTC NCCGAAACGC NCGANNNNAA NNNNNNNNNN
            //
            Primer: EGFP-C-rev
            Region: EGFP 5',PuroR
            Sequence file: RDB09004_A3Cjb.seq check
            >9004_EGFP-C-rev_F01_16.ab1
                1 NNNNNNNNNN NNNNNNTNGC NNNGGGCGGN NTGGGTGCTC NGGTAGTGGT TGTCGGGCAG
               61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
              121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
              181 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
              241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
              301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
              361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
              421 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
              481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
              541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
              601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGCAC CGGGCTTGCG
              661 GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG TCGGCGGTGA CGGTGAAGCC
              721 GAGCCGCTCG TANAAGGGGA GGTTGCGGGG CGCGGANGTC TCCAGGAAGG CGGGCACCCC
              781 GGCGCGCTCG GCCGCCTCCA CTCCGGGGAN CACGACGGCG CTGCCCAGAC CCTTGCCCTN
              841 GTGGTCGGGC GANANGCCGA CGGTGGCCAG GAACCACGCG GGCTCCTTGN GCCGGNGCGG
              901 CGCCAGGNNG NCTTNCATCT GNTGCTGCGC GGCCAGCCNG GAACCGCTCA ACTCNGCCAT
            961 GCGCGGGCCN NTCTCGGCGA ANNCCGCCCC CGCTTCNNNG CNNNCCNNNN NNNNNN
            //
            Primer: COPIApro-F
            Region: copia pro,PuroR
            Sequence file: RDB09004_A3Cjc.seq check
            >09004_09004_B1C4F_COPIApro_F_10_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN CTTTTNNTTC CTTCTTTCCT TCTTGTACGT TTTTTGCTGT
               61 AAGTAGGTCG TGGTGCTGGT GTTGCAGTTG AAATAACTTA AAATATAAAT CATAAAACTC
              121 AAACATAAAC TTGACTATTT ATTTATTTAT TAAGAAAGGA AATATAAATT ATAAATTACA
              181 ACAGATCCCC CGGGCTGCAG GAATTCGATA GCTTGCGCCG CCACCATGAC CGAGTACAAG
              241 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC
              301 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG
              361 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
              421 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
              481 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
              541 CAGCAACAGA TGGAAGGNCT CCTGGCGCCG CACCGGCCCN AGGAGCCCGC GTGGTTCCTG
              601 GCCNCNGTCN GCGTCTCGCC CGANCNCCNN GGCAAGGGTC TGGGCAGCGC CGTCNNGCTC
              661 CCCGNANNNN NNGCNGNNNA NCGNNNNGGN NNNCNNCNTT NCTNNNNANN TNCGNNCCCN
            721 CNNCNNCCNN NNNNNNNN
            //
            Primer: HB-NUS_R
            Region: MT pro,Bip signal seq,V5 tag,6XHis
            Sequence file: RDB09004_A3Cjd.seq check
            >09004_09004_B1C4F_HB-NOS_R_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNGNNNNNNT GGGTNNNGCC NGGGTTTTCC CNGTCACGAC GTTGTAAAAC
               61 GACGGCCAGT GAATTAATTC GTTGCAGGAC AGGNTGTGGT GCCCGATGTG ACTAGCTCTT
              121 TGCTGCAGGC CGTCCTATCC TCTGGTTCCG ATAAGAGACC CAGAACTCCG GCCCCCCACC
              181 GCCCACCGCC ACCCCCATAC ATATGTGGTA CGCAAGTAAG AGTGCCTGCG CATGCCCCAT
              241 GTGCCCCACC AAGAGCTTTG CATCCCATAC AAGTCCCCAA AGTGGAGAAC CGAACCAATT
              301 CTTCGCGGGC AGAACAAAAG CTTCTGCACA CGTCTCCACT CGAATTTGGA GCCGGCCGGC
              361 GTGTGCAAAA GAGGTGAATC GAACGAAAGA CCCGTGTGTA AAGCCGCGTT TCCAAAATGT
              421 ATAAAACCGA GAGCATCTGG CCAATGTGCA TCAGTTGTGG TCAGCAGCAA AATCAAGTGA
              481 ATCATCTCAG TGCAACTAAA GGGGGGATCC GATCTCAATA TGAAGTTATG CATATTACTG
              541 GCCGTCGTGG CCTTTGTTGG CCTCTCGCTC GGGAGATCTT CGCGAACTAG TCCGGATATC
              601 CACGTGTGTA CACCTAGGCT TAAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC
              661 CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA
              721 ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AANGCCTGAN CTCGCTGATC AGCCTCGATC
              781 GAGGANCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
              841 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GANGCTATTG CTTTATTTGT AACCATTATA
              901 AGCTGCAATA AACAAGTTAA CAACNACNAT NGCNTTNNTT TTANGTTTCN NNNTCNGGGG
              961 GANGTGNGGG ANNNTTTTTN NAAGNANGTA AAANNNNNAN NAATGNGGNA NGGCTGANNA
            1021 NGNTCANNNN NNCNNNNNNN NNN
            //
            Primer: V5 Reverse
            Region: Bip signal seq,MT pro
            Sequence file: RDB09004_A3Cje.seq check
            >09004_09004_B1C4F_V5 Reverse_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNTTNNNCC TAGGTGTNNN CNCGTGGNNA TCCGGACTAG TTCGCGAAGA
               61 NCTCCCGAGC GAGAGGCCAA CAAAGGCCAC GACGGCCAGT AATATGCATA ACTTCATATT
              121 GAGATCGGAT CCCCCCTTTA GTTGCACTGA GATGATTCAC TTGATTTTGC TGCTGACCAC
              181 AACTGATGCA CATTGGCCAG ATGCTCTCGG TTTTATACAT TTTGGAAACG CGGCTTTACA
              241 CACGGGTCTT TCGTTCGATT CACCTCTTTT GCACACGCCG GCCGGCTCCA AATTCGAGTG
              301 GAGACGTGTG CAGAAGCTTT TGTTCTGCCC GCGAAGAATT GGTTCGGTTC TCCACTTTGG
              361 GGACTTGTAT GGGATGCAAA GCTCTTGGTG GGGCACATGG GGCATGCGCA GGCACTCTTA
              421 CTTGCGTACC ACATATGTAT GGGGGTGGCG GTGGGCGGTG GGGGGCCGGA GTTCTGGGTC
              481 TCTTATCGGA ACCAGAGGAT AGGACGGCCT GCAGCAAAGA GCTAGTCACA TCGGGCACCA
              541 CATCCTGTCC TGCAACGAAT TAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
              601 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
              661 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
              721 TGGCGCTAGA GGATCCGGTG CGTGGTGGTT TCATGCTTCT GGGAACGGCA AATGGGTTTA
              781 GGANTGGGAA CNNCTCATCA TCTGTTGGAA TATACTATTC AACCTACAAA AGTAACGTTA
              841 AACAACACTA CTTTATATTT GATATGAATG GNCACACCTT TTATGNNNTA AAACATATTG
              901 TAAGANAATA CCACTCTTTT TATNCCTTCT TTCCNNCNNG TANNTTTTTT TGCTGTNNNT
              961 NNNTCNNGNN NNTGGNGNNG CAGTTGAAAT ANNNTAAAAN ATAAATCNNN AAANNNNNNN
            1021 NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nagahashi, K., Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology, 65 (2), 173-178 (2013). PMID 22706964.
            reference Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl