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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-Cre

Expression clone of Cre recombinase.

Catalog number RDB08998
Resource name pCAGGS-Cre
Alternative name stock no 563
Clone info. Expression clone of Cre recombinase under the control of CAG promoter.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name P1 phage Cre recombinase genomic DNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
P1 phage Cre recombinase

          Reference sequence
            
           
          Sequence RDB08998z.seq
          Remarks, protocol and/or map (gif) RDB08998a.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Ogawa, K., Biochem. Biophys. Res. Commun., 343 (1): 159-166 (2006)) designated by the DEPOSITOR is requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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          MTA, for use for not-for-profit academic purpose [Word]
          Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ogawa, K., Biochem. Biophys. Res. Commun., 343 (1): 159-166 (2006))を引用する。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08998 pCAGGS-Cre DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCAGGS-Cre was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08998).

          Reference section:

          Ogawa, K., Nishinakamura, R., Iwamatsu, Y., Shimosato, D., Niwa, H., Synergistic action of Wnt and LIF in maintaining pluripotency of mouse ES cells. Biochem. Biophys. Res. Commun., 343(1): 159-166 (2006). PMID 16530170. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Expression regulation (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08998_A3C7p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3 (Pr0495)
          Region: CAG pro, insert 5'(Cre)
          Sequence file: RDB08998_A3C7c.seq check
          >08998_08998_A3C7_3_pCAG-F_V3_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNTTNNNNNN NNNNNGGNAN GGCGGGGTTC GGCTTCTGGC GTGTGACCGG
             61 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
            121 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTTCGAG GGGCAGAGCC
            181 GATCCTGTAC ACTTTACTTA AAACCATTAT CTGAGTGTGA AATGTCCAAT TTACTGACCG
            241 TACACCAAAA TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT CGCAAGAACC
            301 TGATGGACAT GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA ATGCTTCTGT
            361 CCGTTTGCCG GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG TTTCCCGCAG
            421 AACCTGAAGA TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG GCAGTAAAAA
            481 CTATCCAGCA ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG CTGCCACGAC
            541 CAAGTGACAG CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCNAAAAGAA AACGTTGATG
            601 CCGGTGANCG TGCNAAACAN GNTCTAGCGT TCNAACGCAC TGANTTTCNA NNANGTTNCG
            661 NTCACTCNTG NNAANTANCG ATCGNNNGCN NNGANANNNN NNNANCNNNG NNNNNTNNNN
          721 N
          //
          Primer: pCAGGS_R1 (Pr0614)
          Region: beta-globin pA,insert 3'(Cre)
          Sequence file: RDB08998_A3C7d.seq check
          >08998_08998_A3C7_3_pCAGGS_R1_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNTT GNANTGAAAN NAAATTTCCT TTATTAGCCA GAAGTCAGAT
             61 GCTCAAGGGG CTTCATGATG TCCCCATAAT TTTTGGCAGA GGGAAAAAGA TCTCAGTGGT
            121 ATTTGTGAGC CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT
            181 GAGGAGTGAA TTCGCGTTAA TGGCTAATCG CCATCTTCCA GCAGGCGCAC CATTGCCCCT
            241 GTTTCACTAT CCAGGTTACG GATATAGTTC ATGACAATAT TTACATTGGT CCAGCCACCA
            301 GCTTGCATGA TCTCCGGTAT TGAAACTCCA GCGCGGGCCA TATCTCGCGC GGCTCCGACA
            361 CGGGCACTGT GTCCAGACCA GGCCAGGTAT CTCTGACCAG AGTCATCCTT AGCGCCGTAA
            421 ATCAATCGAT GAGTTGCTTC AAAAATCCCT TCCAGGGCGC GAGTTGATAG CTGGCTGGTG
            481 GCAGATGGCG CGGCAACACC ATTTTTTCTG ACCCGGCAAA ACAGGTAGTT ATTCGGATCA
            541 TCAGCTACAC CAGAGACGGA AATCCATCGC TCGACCAGTT TAGTTACCCC CAGGCTAAGT
            601 GCCTTCTCTA CACCNGCGGT GCTAACCNGC GTTTTCNTTC TGCCAATANG GATTANNNTT
          661 CTCCCNCCGT CAGTACGTGA GANATNNTNA ACCCNGNNNN TNGNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ogawa, K., Synergistic action of Wnt and LIF in maintaining pluripotency of mouse ES cells. Biochem. Biophys. Res. Commun., 343(1): 159-166 (2006). PMID 16530170. [link to RRC of NBRP]
          user_report Tanaka, A., Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells 25 (5): 302-311 (2020). PMID 32065490. [link to RRC of NBRP]
          user_report Serizawa, T., Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Development 146 (21). pii: dev174938 (2019). PMID 31597657. [link to RRC of NBRP]

          2024.01.19

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