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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS-Cre

Expression clone of Cre recombinase.

Catalog number RDB08998
Resource name pCAGGS-Cre
Alternative name stock no 563
Clone info. Expression clone of Cre recombinase under the control of CAG promoter.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name P1 phage Cre recombinase genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Other clones in our bank P1 phage cre |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Ogawa, K., Biochem. Biophys. Res. Commun., 343 (1): 159-166 (2006)) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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RDB08998 pCAGGS-Cre DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08998z.seq
Electronic file Map RDB08998a.gif

Original reference

original Ogawa, K., Synergistic action of Wnt and LIF in maintaining pluripotency of mouse ES cells. Biochem. Biophys. Res. Commun., 343(1): 159-166 (2006). PMID 16530170.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Cre-loxP and FLP-FRT System (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08998_A4G7.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB08998_A4G7a.seq check
>08998_08998_A4G7_pAxCA_F1_F01_16.ab1
    1 TTTAGGTCTG TTAGTAGTGG GCCGACAGGT CTGCACAGTT ATGCCTTCTT CTTTTTCTAC
   61 AGTTTGGGCA ACGTGCTGGT TATTGTGTGA ACAACATACA GTTTTGGCAA AGAATTTCGA
  121 GGGGCAGAGC CGATCCTGTA CACTTTACTT AAAACCATTA TCTGAGTGTG AAATGTCCAA
  181 TTTACTGACC GTACACCAAA ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT
  241 TCGCAAGAAC CTGATGGACA TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA
  301 AATGCTTCTG TCCGTTTGCC GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG
  361 GTTTCCCGCA GAACCTGAAG ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT
  421 GGCAGTAAAA ACTATCCAGC AACATTTGGG CCAGCTAAAC ATGCTTCATC GTCGGTCCGG
  481 GCTGCCACGA CCAAGTGACA GCAATGCTGT TTCACTGGTT ATGCGGCGGA TCCGAAAAGA
  541 AAACGTTGAT GCCGGTGAAC GTGCAAAACA GGCTCTAGCG TTCGAACGCA CTGATTTCGA
  601 CCAGGTTCGT TCACTCATGG AAAATAGCGA TCGCTGCCAG GATATACGTA ATCTGGCATT
  661 TCTGGGGATT GCTTATAACA CCCTGTTACG TATAGCCGAA ATTGCCAGGA TCAGGGTTAA
  721 AGATATCTCA CGTACTGACG GTGGGAGAAT GTTAATCCAT ATTGGCAGAA CGAAAACGCT
  781 GGTTAGCACC GCAGGTGTAG AGAAGGCACT TAGCCTGGGG GTAACTAAAC TGGTCGAGCG
  841 ATGGATTTCC GTCTCTGGTG TAGCTGATGA TCCGAATAAC TACCTGTTTT GCCGGGTTCA
  901 GAAAAAAAAT GGTGTTGACC GCGCCATCTG CCACCAGCCC AGCTATCAAC TTCGCGCCCT
  961 GGAAGGGATT TTTTGAAGGC AACTTCATCG ATTGGATTTT ACGGCCGCCT AAAGGGATGA
 1021 ACTCTTGGGT CAGAGAATAC CTGGGCCCTG GGTTCTGGGA ACAACAGTGG CCCCGTTGTT
 1081 CGGAAGTCCG CGCGGAAGAA TATGGGCCCC GGCGCTTGGG AGTTTCAAAT AACCGGAAGA
1141 TTCAATTGGC CAGGCCTTGT GTGTGGGCCC TTG
//
Primer: pAxCA_R1
Sequence file: RDB08998_A4G7b.seq check
>08998_08998_A4G7_pAxCA_R1_G01_19.ab1
    1 TTTGACAGGT CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA
   61 GTGAATTCGC GTTAATGGCT AATCGCCATC TTCCAGCAGG CGCACCATTG CCCCTGTTTC
  121 ACTATCCAGG TTACGGATAT AGTTCATGAC AATATTTACA TTGGTCCAGC CACCAGCTTG
  181 CATGATCTCC GGTATTGAAA CTCCAGCGCG GGCCATATCT CGCGCGGCTC CGACACGGGC
  241 ACTGTGTCCA GACCAGGCCA GGTATCTCTG ACCAGAGTCA TCCTTAGCGC CGTAAATCAA
  301 TCGATGAGTT GCTTCAAAAA TCCCTTCCAG GGCGCGAGTT GATAGCTGGC TGGTGGCAGA
  361 TGGCGCGGCA ACACCATTTT TTCTGACCCG GCAAAACAGG TAGTTATTCG GATCATCAGC
  421 TACACCAGAG ACGGAAATCC ATCGCTCGAC CAGTTTAGTT ACCCCCAGGC TAAGTGCCTT
  481 CTCTACACCT GCGGTGCTAA CCAGCGTTTT CGTTCTGCCA ATATGGATTA ACATTCTCCC
  541 ACCGTCAGTA CGTGAGATAT CTTTAACCCT GATCCTGGCA ATTTCGGCTA TACGTAACAG
  601 GGTGTTATAA GCAATCCCCA GAAATGCCAG ATTACGTATA TCCTGGCAGC GATCGCTATT
  661 TTCCATGAGT GAACGAACCT GGTCGAAATC AGTGCGTTCG AACGCTAGAG CCTGTTTTGC
  721 ACGTTCACCG GCATCAACGT TTTCTTTTCG GATCCGCCGC ATAACCAGTG AAACAGCATT
  781 GCTGTCACTT GGTCGTGGCA GCCCGGACCG ACGATGAAGC ATGTTTAGCT GGCCCAAATG
  841 TTGCTGGATA GTTTTTTACT GCCAGACCGC GCGCCTGAAG ATATAGAAGA TAATCGCGAA
  901 CATCTTCAGG TTCTGCGGGA AACCATTTCC GGTTATTCAA CTTGCACCAT GCCGCCCACG
  961 ACCGGGCAAA CGGGACAGAA GCATTTTCCA GGTATGCTCA GAAAACGCCT GGCGATCCCT
 1021 GAACATGTCA TCAGTTCTTG CGAACCTTCA TTCACTTCGC TGCCATCGAC TGGATATGCA
1081 GGCAATTTTG CTGTACCGTC AGTAAAATTG GACCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ogawa, K., Synergistic action of Wnt and LIF in maintaining pluripotency of mouse ES cells. Biochem. Biophys. Res. Commun., 343(1): 159-166 (2006). PMID 16530170.
user report Tanaka, A., Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells 2020 Feb 17. doi: 10.1111/gtc.12759. [Epub ahead of print] PMID 32065490.
user report Serizawa, T., Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Development 146 (21). pii: dev174938 (2019). PMID 31597657.

2020.10.02

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