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RBP-J-Venus

Fluorescent reporter clone of Notch signal activation (transcriptional regulation by RBP-J)

Catalog number RDB08964
Resource name RBP-J-Venus
Clone info. Fluorescent reporter clone of Notch signal activation (transcriptional regulation by RBP-J).
Vector backbone pWhere (Plasmid)
Size of vector backbone 9.9kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tanigaki, Kenji |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Marumo T, et al., Neurosci. Res. 75 (3): 204-209, 2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
            Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Marumo T, et al., Neurosci. Res. 75 (3): 204-209, 2013)。非営利機関の非営利学術研究に限って提供する。 
            付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            備考 非営利機関の非営利学術研究に限って提供する。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08964 RBP-J-Venus DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB08964.pdf

            Original reference

            original Marumo, T., Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci. Res., 75 (3): 204-209 (2013). PMID 23396245 .

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Notch signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14702_A7Dip1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pro_F_V2
            Sequence file: RDB14702_A7Dia.seq check
            >08964_14702_A7Di_2_SV40pro_F_V2_H07_22_ABI24.ab1
                1 GTATCAAGCT TTTTTTTGGG AGGCCTAGGC TTTTGCAAAA GCTTGATTTA GGGTGACACT
               61 ATAGAATACA AGCTACTTGT TCTTTTTGCA GGATCGATCC ACCATGGTGA GCAAGGGCGA
              121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
              181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
              241 GCTGATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGGG
              301 CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
              361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
              421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
              481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
              541 CAACAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT
              601 CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC GGCCGACCAC TACCAGCAGA
              661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
              721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGGATCACA ATGGTCCTGC TGGGAGTTTC
              781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGA ATTCTAGAGC
              841 TAGCATTATC CCTAATACCT GCCACCCCAC TCTTAATCCA GTGGTGGGAA GAACGGTCTC
              901 AGAACTGTTT TGTTTCAAAT TGGCCATTTT AAGTTTAGTA GTAAAAAGAC TGGGTTTAAT
              961 GGATAACCAT GGCATCGTAA AACCTTTCAG GAAGGGAAAG GAGGAAATGT TTTGGTGGAA
             1021 CCACCTTTGG GTTTTCTTTT TTTTGCCGGT GTGGGCAGTT TTAAAGGTAA TTAAGTTTTT
             1081 GAAATTCGAG TACCTTTTTT AATGGGAAAC AACCTTGGAC CAAAAATTTT GTTCCACGGA
            1141 TTTTGAGACC CATTAGAAAA AGGTTAATTG GAAAAGAACA CCTGTGTGT
            //
            Primer: Venus-N_R
            Sequence file: RDB14702_A7Dib.seq check
            >08964_14702_A7Di_2_Venus-N_R_C08_08_ABI24.ab1
                1 GCGTCAGACG CTGACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
               61 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG ATCGATCCTG CAAAAAGAAC
              121 AAGTAGCTTG TATTCTATAG TGTCACCTAA ATCAAGCTTT TTGCAAAAGC CTAGGCCTCC
              181 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC
              241 ATAAATAAAA AAAATTAGTC AGCGATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG
              301 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCAGATC
              361 GCAGATCTGG ATCCCCTGGA ACTATTTTCC CACGGTGCCC TTCCGCCCAT TTTCCCACGA
              421 GTCGCGAGCC TGGAACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGCG
              481 AGCTCGAGAG GATCCACCTA GGACCTGCAG GAGCCATTGC TACAGTTCCC GAATCACCAC
              541 AAGGAAAGAA AAAGGTTGGT GAGAAAATAG AGATTCTATT TTCATGTCCG GGGGATGAGC
              601 GTGTAGGGCA CTTACACCCA GGACTCAAAG GAACATGCTA CATTCACACG AGCATCCAGG
              661 AGGCATAAGA ATTCTGCAAG GAGACCATGC CCTATTCTTG GACGTCTGTT GAATCAGTTG
              721 TGGGGTTTAT ACGCGGGAGT TGCCGCGTGG TGGCAACAAA ATCGATTGCG CCAAACCTAA
              781 AGAGCCCCCC CACCCCTGGT ATTGGAATTC ACAAATGGCA ATGCTGTGGG TCACCCAAGT
              841 TCAGTACCTC AGGGGGGTCA CAAATGCCAC TAGGGGGGGC AGGACACATG CATTTTCTAG
              901 GCTGGTACCT CGTGGACTCG GACTCCCAAA TCAACAAGTC GGCTTACTCT CTGCAAAGAA
              961 TCCCTTTGTG TGTAAAGACC AGGTTTGCCG CACGGCGGCA AGTGAAGTCT CGTACATCGC
            1021 AGTCCTAAAA AC
            //
            Primer: Venus-C_F2
            Sequence file: RDB14702_A7Dic.seq check
            >08964_14702_A7Di_2_Venus-C_F2_H10_22_ABI24.ab1
                1 CGTTTCGGTC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCTA
               61 GAGCTAGCAT TATCCCTAAT ACCTGCCACC CCACTCTTAA TCAGTGGTGG AAGAACGGTC
              121 TCAGAACTGT TTGTTTCAAT TGGCCATTTA AGTTTAGTAG TAAAAGACTG GTTAATGATA
              181 ACAATGCATC GTAAAACCTT CAGAAGGAAA GGAGAATGTT TTGTGGACCA CTTTGGTTTT
              241 CTTTTTTGCG TGTGGCAGTT TTAAGTTATT AGTTTTTAAA ATCAGTACTT TTTAATGGAA
              301 ACAACTTGAC CAAAAATTTG TCACAGAATT TTGAGACCCA TTAAAAAAGT TAAATGAGAA
              361 ACCTGTGTGT TCCTTTGGTC AACACCGAGA CATTTAGGTG AAAGACATCT AATTCTGGTT
              421 TTACGAATCT GGAAACTTCT TGAAAATGTA ATTCTTGAGT TAACACTTCT GGGTGGAGAA
              481 TAGGGTTGTT TTCCCCCCAC ATAATTGGAA GGGGAAGGAA TATCATTTAA AGCTATGGGA
              541 GGGTTGCTTT GATTACAACA CTGGAGAGAA ATGCAGCATG TTGCTGATTG CCTGTCACTA
              601 AAACAGGCCA AAAACTGAGT CCTTGGGTTG CATAGAAAGC TGCCTGCAGA GCTTTGTCAC
              661 AGCGGGACCC CAACCTATGC CGCGTCTGCC GAGCAATATG TAGGTATTGT ACTGCCACCA
              721 GGCGGCATCG TCTGTCCATT TAAGCTATAG CCAAATCTGC ACAAGCGTGG AGAGTGAACC
              781 GAATCGATCG TGAAAGGCGC AGAACCGAAG GAGCTACCCA AGAAATGTGT GTTGTACCAC
              841 CCCCATGACC TAATGAAATC ATTGAAATGC TATGCCTGAG TGAACCAATG AGTTTGGCCA
              901 TAGTGGAACC GCAAATTTTG GTCACCTCTA TTGATAAAAG TATGTGGGGA CTGGCACTTG
              961 GTTCGCTTGC TCGGCAACTC CCGGTTCTTA CCAGCCACTG GAGCGATTCC TCGGCTGTGG
             1021 TAAGGAATGG AGTCCAAGTC TCTCTGGGTT CAGGTGGTAA GAACCATTCC AAGGTGCACC
            1081 ATCTAACACC TATGGATTCC CTATTATTTG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Marumo, T., Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci. Res., 75 (3): 204-209 (2013). PMID 23396245 .

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl