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RBP-J-Venus

Fluorescent reporter clone of Notch signal activation (transcriptional regulation by RBP-J)

Catalog number RDB08964
Resource name RBP-J-Venus
Clone info. Fluorescent reporter clone of Notch signal activation (transcriptional regulation by RBP-J).
Vector backbone pWhere (Plasmid)
Size of vector backbone 9.9kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Tanigaki, Kenji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Marumo T, et al., Neurosci. Res. 75 (3): 204-209, 2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus.
Remarks The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08964 RBP-J-Venus DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB08964.pdf from Depositor

Featured content

Featured content Fluorescent reporter clone of Notch signal activation (English text)
Featured content Notch signaling pathway (English text)

References

original Marumo, T., Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci. Res., 75 (3): 204-209 (2013). PMID 23396245 .

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14702_A7Dip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pro_F_V2 RDB14702_A7Dia.seq
>08964_14702_A7Di_2_SV40pro_F_V2_H07_22_ABI24.ab1
    1 GTATCAAGCT TTTTTTTGGG AGGCCTAGGC TTTTGCAAAA GCTTGATTTA GGGTGACACT
   61 ATAGAATACA AGCTACTTGT TCTTTTTGCA GGATCGATCC ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GCTGATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGGG
  301 CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC GGCCGACCAC TACCAGCAGA
  661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
  721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGGATCACA ATGGTCCTGC TGGGAGTTTC
  781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGA ATTCTAGAGC
  841 TAGCATTATC CCTAATACCT GCCACCCCAC TCTTAATCCA GTGGTGGGAA GAACGGTCTC
  901 AGAACTGTTT TGTTTCAAAT TGGCCATTTT AAGTTTAGTA GTAAAAAGAC TGGGTTTAAT
  961 GGATAACCAT GGCATCGTAA AACCTTTCAG GAAGGGAAAG GAGGAAATGT TTTGGTGGAA
 1021 CCACCTTTGG GTTTTCTTTT TTTTGCCGGT GTGGGCAGTT TTAAAGGTAA TTAAGTTTTT
 1081 GAAATTCGAG TACCTTTTTT AATGGGAAAC AACCTTGGAC CAAAAATTTT GTTCCACGGA
1141 TTTTGAGACC CATTAGAAAA AGGTTAATTG GAAAAGAACA CCTGTGTGT
//
Primer: Venus-N_R RDB14702_A7Dib.seq
>08964_14702_A7Di_2_Venus-N_R_C08_08_ABI24.ab1
    1 GCGTCAGACG CTGACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
   61 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG ATCGATCCTG CAAAAAGAAC
  121 AAGTAGCTTG TATTCTATAG TGTCACCTAA ATCAAGCTTT TTGCAAAAGC CTAGGCCTCC
  181 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC
  241 ATAAATAAAA AAAATTAGTC AGCGATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG
  301 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCAGATC
  361 GCAGATCTGG ATCCCCTGGA ACTATTTTCC CACGGTGCCC TTCCGCCCAT TTTCCCACGA
  421 GTCGCGAGCC TGGAACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGCG
  481 AGCTCGAGAG GATCCACCTA GGACCTGCAG GAGCCATTGC TACAGTTCCC GAATCACCAC
  541 AAGGAAAGAA AAAGGTTGGT GAGAAAATAG AGATTCTATT TTCATGTCCG GGGGATGAGC
  601 GTGTAGGGCA CTTACACCCA GGACTCAAAG GAACATGCTA CATTCACACG AGCATCCAGG
  661 AGGCATAAGA ATTCTGCAAG GAGACCATGC CCTATTCTTG GACGTCTGTT GAATCAGTTG
  721 TGGGGTTTAT ACGCGGGAGT TGCCGCGTGG TGGCAACAAA ATCGATTGCG CCAAACCTAA
  781 AGAGCCCCCC CACCCCTGGT ATTGGAATTC ACAAATGGCA ATGCTGTGGG TCACCCAAGT
  841 TCAGTACCTC AGGGGGGTCA CAAATGCCAC TAGGGGGGGC AGGACACATG CATTTTCTAG
  901 GCTGGTACCT CGTGGACTCG GACTCCCAAA TCAACAAGTC GGCTTACTCT CTGCAAAGAA
  961 TCCCTTTGTG TGTAAAGACC AGGTTTGCCG CACGGCGGCA AGTGAAGTCT CGTACATCGC
1021 AGTCCTAAAA AC
//
Primer: Venus-C_F2 RDB14702_A7Dic.seq
>08964_14702_A7Di_2_Venus-C_F2_H10_22_ABI24.ab1
    1 CGTTTCGGTC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCTA
   61 GAGCTAGCAT TATCCCTAAT ACCTGCCACC CCACTCTTAA TCAGTGGTGG AAGAACGGTC
  121 TCAGAACTGT TTGTTTCAAT TGGCCATTTA AGTTTAGTAG TAAAAGACTG GTTAATGATA
  181 ACAATGCATC GTAAAACCTT CAGAAGGAAA GGAGAATGTT TTGTGGACCA CTTTGGTTTT
  241 CTTTTTTGCG TGTGGCAGTT TTAAGTTATT AGTTTTTAAA ATCAGTACTT TTTAATGGAA
  301 ACAACTTGAC CAAAAATTTG TCACAGAATT TTGAGACCCA TTAAAAAAGT TAAATGAGAA
  361 ACCTGTGTGT TCCTTTGGTC AACACCGAGA CATTTAGGTG AAAGACATCT AATTCTGGTT
  421 TTACGAATCT GGAAACTTCT TGAAAATGTA ATTCTTGAGT TAACACTTCT GGGTGGAGAA
  481 TAGGGTTGTT TTCCCCCCAC ATAATTGGAA GGGGAAGGAA TATCATTTAA AGCTATGGGA
  541 GGGTTGCTTT GATTACAACA CTGGAGAGAA ATGCAGCATG TTGCTGATTG CCTGTCACTA
  601 AAACAGGCCA AAAACTGAGT CCTTGGGTTG CATAGAAAGC TGCCTGCAGA GCTTTGTCAC
  661 AGCGGGACCC CAACCTATGC CGCGTCTGCC GAGCAATATG TAGGTATTGT ACTGCCACCA
  721 GGCGGCATCG TCTGTCCATT TAAGCTATAG CCAAATCTGC ACAAGCGTGG AGAGTGAACC
  781 GAATCGATCG TGAAAGGCGC AGAACCGAAG GAGCTACCCA AGAAATGTGT GTTGTACCAC
  841 CCCCATGACC TAATGAAATC ATTGAAATGC TATGCCTGAG TGAACCAATG AGTTTGGCCA
  901 TAGTGGAACC GCAAATTTTG GTCACCTCTA TTGATAAAAG TATGTGGGGA CTGGCACTTG
  961 GTTCGCTTGC TCGGCAACTC CCGGTTCTTA CCAGCCACTG GAGCGATTCC TCGGCTGTGG
 1021 TAAGGAATGG AGTCCAAGTC TCTCTGGGTT CAGGTGGTAA GAACCATTCC AAGGTGCACC
1081 ATCTAACACC TATGGATTCC CTATTATTTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Marumo, T., Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci. Res., 75 (3): 204-209 (2013). PMID 23396245 .

2019.01.08

GNP_filter3_RDBDEP_html_181012.pl