Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

RBP-J-Venus (#RDB08964)

Fluorescent reporter clone of Notch signal activation (transcriptional regulation by RBP-J)

Clone info. Fluorescent reporter clone of Notch signal activation (transcriptional regulation by RBP-J).
Vector backbone pWhere (Plasmid)
Size of vector backbone 9.9kb
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tanigaki, Kenji |
 
Remarks, protocol and/or map (pdf) RDB08964.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Marumo T, et al., Neurosci. Res. 75 (3): 204-209, 2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Marumo T, et al., Neurosci. Res. 75 (3): 204-209, 2013)。非営利機関の非営利学術研究に限って提供する。　
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08964 RBP-J-Venus DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The RBP-J-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08964).

Reference section:

Marumo, T., Takagi, Y., Muraki, K., Hashimoto, N., Miyamoto, S., Tanigaki, K., Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci. Res., 75 (3): 204-209 (2013). PMID 23396245. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14702_A7Dip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pro_F_V2
Sequence file: RDB14702_A7Dia.seq check
>08964_14702_A7Di_2_SV40pro_F_V2_H07_22_ABI24.ab1
    1 GTATCAAGCT TTTTTTTGGG AGGCCTAGGC TTTTGCAAAA GCTTGATTTA GGGTGACACT
   61 ATAGAATACA AGCTACTTGT TCTTTTTGCA GGATCGATCC ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GCTGATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGGG
  301 CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC GGCCGACCAC TACCAGCAGA
  661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
  721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGGATCACA ATGGTCCTGC TGGGAGTTTC
  781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGA ATTCTAGAGC
  841 TAGCATTATC CCTAATACCT GCCACCCCAC TCTTAATCCA GTGGTGGGAA GAACGGTCTC
  901 AGAACTGTTT TGTTTCAAAT TGGCCATTTT AAGTTTAGTA GTAAAAAGAC TGGGTTTAAT
  961 GGATAACCAT GGCATCGTAA AACCTTTCAG GAAGGGAAAG GAGGAAATGT TTTGGTGGAA
 1021 CCACCTTTGG GTTTTCTTTT TTTTGCCGGT GTGGGCAGTT TTAAAGGTAA TTAAGTTTTT
 1081 GAAATTCGAG TACCTTTTTT AATGGGAAAC AACCTTGGAC CAAAAATTTT GTTCCACGGA
1141 TTTTGAGACC CATTAGAAAA AGGTTAATTG GAAAAGAACA CCTGTGTGT
//
Primer: Venus-N_R
Sequence file: RDB14702_A7Dib.seq check
>08964_14702_A7Di_2_Venus-N_R_C08_08_ABI24.ab1
    1 GCGTCAGACG CTGACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
   61 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG ATCGATCCTG CAAAAAGAAC
  121 AAGTAGCTTG TATTCTATAG TGTCACCTAA ATCAAGCTTT TTGCAAAAGC CTAGGCCTCC
  181 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC
  241 ATAAATAAAA AAAATTAGTC AGCGATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG
  301 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCAGATC
  361 GCAGATCTGG ATCCCCTGGA ACTATTTTCC CACGGTGCCC TTCCGCCCAT TTTCCCACGA
  421 GTCGCGAGCC TGGAACTATT TTCCCACGGT GCCCTTCCGC CCATTTTCCC ACGAGTCGCG
  481 AGCTCGAGAG GATCCACCTA GGACCTGCAG GAGCCATTGC TACAGTTCCC GAATCACCAC
  541 AAGGAAAGAA AAAGGTTGGT GAGAAAATAG AGATTCTATT TTCATGTCCG GGGGATGAGC
  601 GTGTAGGGCA CTTACACCCA GGACTCAAAG GAACATGCTA CATTCACACG AGCATCCAGG
  661 AGGCATAAGA ATTCTGCAAG GAGACCATGC CCTATTCTTG GACGTCTGTT GAATCAGTTG
  721 TGGGGTTTAT ACGCGGGAGT TGCCGCGTGG TGGCAACAAA ATCGATTGCG CCAAACCTAA
  781 AGAGCCCCCC CACCCCTGGT ATTGGAATTC ACAAATGGCA ATGCTGTGGG TCACCCAAGT
  841 TCAGTACCTC AGGGGGGTCA CAAATGCCAC TAGGGGGGGC AGGACACATG CATTTTCTAG
  901 GCTGGTACCT CGTGGACTCG GACTCCCAAA TCAACAAGTC GGCTTACTCT CTGCAAAGAA
  961 TCCCTTTGTG TGTAAAGACC AGGTTTGCCG CACGGCGGCA AGTGAAGTCT CGTACATCGC
1021 AGTCCTAAAA AC
//
Primer: Venus-C_F2
Sequence file: RDB14702_A7Dic.seq check
>08964_14702_A7Di_2_Venus-C_F2_H10_22_ABI24.ab1
    1 CGTTTCGGTC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCTA
   61 GAGCTAGCAT TATCCCTAAT ACCTGCCACC CCACTCTTAA TCAGTGGTGG AAGAACGGTC
  121 TCAGAACTGT TTGTTTCAAT TGGCCATTTA AGTTTAGTAG TAAAAGACTG GTTAATGATA
  181 ACAATGCATC GTAAAACCTT CAGAAGGAAA GGAGAATGTT TTGTGGACCA CTTTGGTTTT
  241 CTTTTTTGCG TGTGGCAGTT TTAAGTTATT AGTTTTTAAA ATCAGTACTT TTTAATGGAA
  301 ACAACTTGAC CAAAAATTTG TCACAGAATT TTGAGACCCA TTAAAAAAGT TAAATGAGAA
  361 ACCTGTGTGT TCCTTTGGTC AACACCGAGA CATTTAGGTG AAAGACATCT AATTCTGGTT
  421 TTACGAATCT GGAAACTTCT TGAAAATGTA ATTCTTGAGT TAACACTTCT GGGTGGAGAA
  481 TAGGGTTGTT TTCCCCCCAC ATAATTGGAA GGGGAAGGAA TATCATTTAA AGCTATGGGA
  541 GGGTTGCTTT GATTACAACA CTGGAGAGAA ATGCAGCATG TTGCTGATTG CCTGTCACTA
  601 AAACAGGCCA AAAACTGAGT CCTTGGGTTG CATAGAAAGC TGCCTGCAGA GCTTTGTCAC
  661 AGCGGGACCC CAACCTATGC CGCGTCTGCC GAGCAATATG TAGGTATTGT ACTGCCACCA
  721 GGCGGCATCG TCTGTCCATT TAAGCTATAG CCAAATCTGC ACAAGCGTGG AGAGTGAACC
  781 GAATCGATCG TGAAAGGCGC AGAACCGAAG GAGCTACCCA AGAAATGTGT GTTGTACCAC
  841 CCCCATGACC TAATGAAATC ATTGAAATGC TATGCCTGAG TGAACCAATG AGTTTGGCCA
  901 TAGTGGAACC GCAAATTTTG GTCACCTCTA TTGATAAAAG TATGTGGGGA CTGGCACTTG
  961 GTTCGCTTGC TCGGCAACTC CCGGTTCTTA CCAGCCACTG GAGCGATTCC TCGGCTGTGG
 1021 TAAGGAATGG AGTCCAAGTC TCTCTGGGTT CAGGTGGTAA GAACCATTCC AAGGTGCACC
1081 ATCTAACACC TATGGATTCC CTATTATTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marumo, T., Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke. Neurosci. Res., 75 (3): 204-209 (2013). PMID 23396245. [link to RRC of NBRP]