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Expression vector of HIV-1 gag and pol for lentivirus vector packaging. Integrase mutant.
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Other clones in our bank
External Database HIV-1 gag-pol Reference sequence |
| Sequence | RDB08943z.seq |
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| Remarks, protocol and/or map (pdf) | RDB08943.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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| Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ] |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| 提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
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| 提供依頼 | 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word ]提供同意書 (非営利機関による非営利学術研究用)[Word ]遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 |
| 備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB08943 | pCAG-HIVgp IN-D64E | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pCAG-HIVgp IN-D64E was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08943). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Lentivirus Vector (English text) |
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| Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB08943_A3Flp1-3.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pAxCA_F1 (Pr0164) Region: insert 5'(HIV-1 gag 5') Sequence file: RDB08943_A3Fla.seq
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>08943_08943_A3Fl_pAxCA_F1_G07_19.ab1 1 CAAGGACAAA CAATACTTCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC 61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTTG ATACGTACGG 121 TTTCGAGCTC AAGCTTCGAA TTCGAGATCT GCCGCCGCCA TGGGTGCGAG AGCGTCAGTA 181 TTAAGCGGGG GAGAATTAGA TCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA 241 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT 301 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC 361 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT 421 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG 481 CAAAACAAAA GTAAGAAAAA AGCACAGCAA GCAGCAGCTG ACACAGGACA CAGCAATCAG 541 GTCAGCCAAA ATTACCCTAT AGTGCAGAAC ATCCAGGGGC AAATGGTACA TCAGGCCATA 601 TCACCTAGAA CTTTAAATGC ATGGGTAAAA GTAGTAGAAG AGAAGGCTTT CAGCCCAGAA 661 GTGATACCCA TGTTTTCAGC ATTATCAGAA GGAGCCACCC CACAAGATTT AAACACCATG 721 CTAAACACAG TGGGGGGACA TCAAGCAGCC ATGCAAATGT TAAAAGAGAC CATCAATGAG 781 GAAGCTGCAG AATGGGATAG AGTGCATCCA GTGCATGCAG GGCCTATTGC ACCAGGCCAG 841 ATGAGAGAAC CAAGGGGAAG TGACATAGCA GGAACTACTA GTACCCTTCA GGAACAAATA 901 GGATGGATGA CACATAATCC ACCTATCCCA GTAGGAGAAA TCTATAAAAG ATGGATAATC 961 CTGGGATTAA ATAAAATAGT AAGAATGTAT AGCCCTACCA GCATTCTGGA CATAAGACAA 1021 GGACCAAAGG AACCCTTTAG AGACTATGTA GACCGATTCT ATAAAACTCT AGAGCCGAGC 1081 AGCTTCACAG AGGTAAAAAA TGATGACAGA ACCTTGATGG TCCAAATGCG ACCCGGATGG 1141 TAGGACTATT TAAAGCATTT GGGGACCAGG AGCCGAACAC CTTAGAAGAA AAAAAG // |
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Primer: HIVgp_R1 (Pr0132) Region: insert 3'(HIV-1 pol 3') Sequence file: RDB08943_A3Flb.seq
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>08943_08943_A3Fl_HIVgp-R1_H05_23.ab1 1 GCGGCAATCT GCGGGCCGCT CCGGATTCCA TGTGTTAATC CTCATCCTGT CTACTTGCCA 61 CACAATCATC ACCTGCCATC TGTTTTCCAT AATCCCTGAT GATCTTTGCT TTTCTTCTTG 121 GCACTACTTT TATGTCACTA TTATCTTGTA TTACTACTGC CCCTTCACCT TTCCAGAGGA 181 GCTTTGCTGG TCCTTTCCAA ACTGGATCTC TGCTGTCCCT GTAATAAACC CGAAAATTTT 241 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA 301 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA 361 ATACTGCCAT TTGTACTGCT GTCTTAAGAT GTTCAGCCTG ATCTCTTACC TGTCCTATAA 421 TTTTCTTTAA TTCTTTATTC ATAGATTCTA TTACTCCTTG ACTTTGGGGA TTGTAGGGAA 481 TGCCAAATTC CTGCTTGATC CCCGCCCACC AACAGGCGGC CTTAACTGTA GTACTGGTGA 541 AATTGCTGCC ATTGTCTGTA TGTACTGTTT TTACTGGCCA TCTTCCTGCT AATTTTAAGA 601 GGAAGTATGC TGTTTCTTGC CCTGTCTCTG CTGGAATTAC TTCTGCTTCT ATATATCCAC 661 TGGCTACATG AACTGCTACC AAGATAACTT TTCCTTCTAA ATGTGTGCAC TCTAGCTGCC 721 ATATTCCTGG GCTACAGTCT ACTTGTCCAT GCATGGCTTC CCCTTTTAGC TGACATTTAT 781 CACAGCTGGC TACTATTTCT TTTGCTACTA CAGGTGGTAG GTTAAAATCA CTAGCCATTG 841 CTCTCCAATT ACTGTGATAT TTCTCATGTT CTTCTTGGGC CTTATCTATT CCATCTAAAA 901 AATAGTACTT TCCCTGATTC CAGCACTGAC CAAATTTATC TACTTGTTCA TTTTCCTCCA 961 AATTCCCTTT GTGTGCTGGG TACCCCATGC CCAGGTAGAC TTTTTTTCCT TTTTTATTAA 1021 CTGCTCTATT ATTGACTGAC TAACTCTGAT TCACTTCTAA TCTGGATGTT GCTTGAATGG 1081 ATTCCATTGC ATATTGTTGA GTCCTGTTCC ACCCAATG // |
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Primer: SV40pro_F (Pr0110) Region: SV 40 pro_ori,NeoR_KanR Sequence file: RDB08943_A3Flc.seq
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>08943_08943_A3Fl_SV40pro_F_A01_01_ABI08.ab1 1 CAGGGGGGGT CGGCATCCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT 301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 601 AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGGACTC 901 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCCAACCTGC CATCACGAGA TTTCCGATTC 961 CACCGCCCGC CTTTCTATGA AAAGGGTTGG GCTTTCGGAA TCGTTTTCCG GACGCCCGGC 1021 TGGAATGGAT TCTCCCAGCG CGGGGGAATC TCATGCTTGG AGTTCTTCGT CCACCCTAGG 1081 GAGGCCTACT GGAACACGGT AAGGAGACTA AATACCCCGC AAGTG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2023.05.01
GNP_filter3_RDBDEP_html_230501.pl