Resource data sheet
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pCAG-HIVgp IN-D64E

Expression vector of VSV G protein HIV-1 gag and pol. Integrase mutant.

Catalog number RDB08943
Resource name pCAG-HIVgp IN-D64E
Clone info. Expression vector of VSV G protein HIV-1 gag and po for packagingl. Integrase mutant.
Vector backbone Plasmid
Selectable markers Kan^r
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08943_A3Fl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB08943_A3Fla.seq
>08943_08943_A3Fl_pAxCA_F1_G07_19.ab1
    1 CAAGGACAAA CAATACTTCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC
   61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTTG ATACGTACGG
  121 TTTCGAGCTC AAGCTTCGAA TTCGAGATCT GCCGCCGCCA TGGGTGCGAG AGCGTCAGTA
  181 TTAAGCGGGG GAGAATTAGA TCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA
  241 AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT
  301 CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC
  361 CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT
  421 GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG
  481 CAAAACAAAA GTAAGAAAAA AGCACAGCAA GCAGCAGCTG ACACAGGACA CAGCAATCAG
  541 GTCAGCCAAA ATTACCCTAT AGTGCAGAAC ATCCAGGGGC AAATGGTACA TCAGGCCATA
  601 TCACCTAGAA CTTTAAATGC ATGGGTAAAA GTAGTAGAAG AGAAGGCTTT CAGCCCAGAA
  661 GTGATACCCA TGTTTTCAGC ATTATCAGAA GGAGCCACCC CACAAGATTT AAACACCATG
  721 CTAAACACAG TGGGGGGACA TCAAGCAGCC ATGCAAATGT TAAAAGAGAC CATCAATGAG
  781 GAAGCTGCAG AATGGGATAG AGTGCATCCA GTGCATGCAG GGCCTATTGC ACCAGGCCAG
  841 ATGAGAGAAC CAAGGGGAAG TGACATAGCA GGAACTACTA GTACCCTTCA GGAACAAATA
  901 GGATGGATGA CACATAATCC ACCTATCCCA GTAGGAGAAA TCTATAAAAG ATGGATAATC
  961 CTGGGATTAA ATAAAATAGT AAGAATGTAT AGCCCTACCA GCATTCTGGA CATAAGACAA
 1021 GGACCAAAGG AACCCTTTAG AGACTATGTA GACCGATTCT ATAAAACTCT AGAGCCGAGC
 1081 AGCTTCACAG AGGTAAAAAA TGATGACAGA ACCTTGATGG TCCAAATGCG ACCCGGATGG
1141 TAGGACTATT TAAAGCATTT GGGGACCAGG AGCCGAACAC CTTAGAAGAA AAAAAG
//
Primer: RDB08943_A3Flb.seq
>08943_08943_A3Fl_HIVgp-F1_G05_20.ab1
    1 GCAAGGTGGC GGACTCACAA TATGCATTGG GAATCATTCA AGCACAACCA GATAAGAGTG
   61 AATCAGAGTT AGTCAGTCAA ATAATAGAGC AGTTAATAAA AAAGGAAAAA GTCTACCTGG
  121 CATGGGTACC AGCACACAAA GGAATTGGAG GAAATGAACA AGTAGATAAA TTGGTCAGTG
  181 CTGGAATCAG GAAAGTACTA TTTTTAGATG GAATAGATAA GGCCCAAGAA GAACATGAGA
  241 AATATCACAG TAATTGGAGA GCAATGGCTA GTGATTTTAA CCTACCACCT GTAGTAGCAA
  301 AAGAAATAGT AGCCAGCTGT GATAAATGTC AGCTAAAAGG GGAAGCCATG CATGGACAAG
  361 TAGACTGTAG CCCAGGAATA TGGCAGCTAG AGTGCACACA TTTAGAAGGA AAAGTTATCT
  421 TGGTAGCAGT TCATGTAGCC AGTGGATATA TAGAAGCAGA AGTAATTCCA GCAGAGACAG
  481 GGCAAGAAAC AGCATACTTC CTCTTAAAAT TAGCAGGAAG ATGGCCAGTA AAAACAGTAC
  541 ATACAGACAA TGGCAGCAAT TTCACCAGTA CTACAGTTAA GGCCGCCTGT TGGTGGGCGG
  601 GGATCAAGCA GGAATTTGGC ATTCCCTACA ATCCCCAAAG TCAAGGAGTA ATAGAATCTA
  661 TGAATAAAGA ATTAAAGAAA ATTATAGGAC AGGTAAGAGA TCACGCTGAA CATCTTAAGA
  721 CAGCAGTACA AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG ATTGGGGGGG
  781 TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA
  841 CAAAAACAAT TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGGACAGC AGAGATCCAG
  901 TTTGGAAAGG ACCAGCAAAG CTCCTCTGGA AAGGTGAAGG GGCAGTAGTA ATACAAGAAT
  961 AATAGTGAAC ATAAAAAGTA GTGCTAGAAG AAGGCAAGAT CATCAGGATT ATGGAAAACA
 1021 GATGCAAGGT GATGATGTGT GCCAGTAGAA CAGGGAATGA GATACCATTG TAATCCGGAA
1081 CGGCCGGCAA GAGGAGCT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References


2017.10.23

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