RDB08938 - pCAGGS

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pCAGGS

Mammalian expression vector with CAG promoter.

Catalog number	RDB08938
Resource name	pCAGGS
Alternative name	stock no 36
Clone info.	Mammalian expression vector with CAG promoter.
Comment	EcoRI and XhoI sites are available for cloning cDNA.
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r
Depositor\|Developer	Niwa, Hitoshi \|

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External Database

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Niwa, H., Gene. 108 (2), 193-200 (1991)).

Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Niwa, H., Gene. 108 (2), 193-200 (1991)).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Niwa, H., Gene. 108 (2), 193-200 (1991))。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08938	pCAGGS	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB08938z.seq
Remarks, protocol and/or map (gif)	RDB08938a.gif

Original reference

original	Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.

Further references such as user reports and related articles (go to bottom)

Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08938_A4Afp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 Region: CAG pro,beta-globin p(A) Sequence file: RDB08938_A4Afc.seq
>08938_08938_A9D4_CAG_R1_beta_F09_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCTCNAGCG GGNGCGGGGC GAANCGGTGC GGCGCCGGCA 61 GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC 121 TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG 181 GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT 241 TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG 301 GCAAAGAATT CCTCGAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG 361 CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC TCTGCCAAAA 421 ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA 481 TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA 541 GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGCCC 601 ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC 661 CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTNN 721 NNNNTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC 781 TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT 841 NGANNTNCCT CNANCTGCAN CCNANGCTTG GCGTAATCAT GGNCNTAGCT GTTNNCTGTG 901 TGNAANNTNT TATCCGCTCN CNNTTNCNCN NNACANNCNN NNNCNGAANC ANNAANTNNN 961 ANNNCNNGGG GGNGNCNAAN NGANNNNNNC NNANNNNNNN NNN //
Primer: SV40pA3'element Reverse Region: SV40p(A),SV40 pro_ori Sequence file: RDB08938_A4Afd.seq
>08938_08938_A4Af_SV40pA3'Element Reverse_F05_17.ab1 1 NNNNNNNNNN NNGGNNNNNN NNNNNGNNNN NNGCNNGCAA CGCGGCCTTT TTACGGTTCC 61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 241 CGCGTTGGCC GATTCATTAA TGCAGCGGAT CCAGACATGA TAAGATACAT TGATGAGTTT 301 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT 361 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAGCTTTTT GCAAAAGCCT 421 AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG 481 GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC 541 GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA 601 TGCGGATCCG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA 661 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 721 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACNNTG 781 ATTACGCCAA GCTTGGGCTG CAGGTCGANG GATCTCCNTA AGAGAAGANG GACNGCTATG 841 ACTGGNNNTA GTCAGGANAN GAGGAAAAAT CTGGCTAGTA AANNATGTNA GGAAAATTTT 901 AGGNNNGTTA AAGAAAAAAA TAACNCAAAN CAAAANATAA AAAAAATCTA ACCTCAAGTC 961 NANGGCTTTT CTANNGNAAN ANNNAATGGN NAGNNNGGGG GNNNNNTTNN NTANNCTNNN 1021 GANCNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
user_report	Senoo, A., Orthogonal Activation of Metabotropic Glutamate Receptor Using Coordination Chemogenetics. Front. Chem. 9: 825669 (2022). PMID 35096780.
user_report	Yamanaka, S., Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF. EMBO J. 40 (4): e105375 (2021). PMID 33470442.
user_report	Wibisono, H., Tracing location by applying Emerald luciferase in an early phase of murine endometriotic lesion formation. Exp Anim. 2021 Nov 25. doi: 10.1538/expanim.21-0146. Epub ahead of print. PMID 34819403.
user_report	Yamanashi, T., Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination. Sci. Rep. 9 (1): 7863 (2019). PMID 31133660.
user_report	Ide, M., Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. EMBO Mol. Med. 28: e10695 (2019). PMID 31657521.
user_report	Ueda, Y., O-GlcNAcylation-mediated degradation of FBXL2 stabilizes FOXM1 to induce cancer progression. Biochem. Biophys. Res. Commun. pii: S0006-291X (19) 32074-1 (2019). PMID 31679690.
user_report	Nihongaki, Y., CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat. Methods 14 (10): 963-966 (2017). PMID 28892089.
user_report	Yahata, N., TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation. Sci. Rep. 7 (1): 15557 (2017). PMID 29138463.
user_report	Yoshimi, K., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405.
user_report	Yamaura, K., Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry. Nat. Chem. Biol. 12 (10): 822-830 (2016). PMID 27526031.

2022.05.17

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