Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS

Mammalian expression vector with CAG promoter.

Catalog number RDB08938
Resource name pCAGGS
Alternative name stock no 36
Clone info. Mammalian expression vector with CAG promoter.
Comment EcoRI and XhoI sites are available for cloning cDNA.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Niwa, H., Gene. 108 (2), 193-200 (1991)).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Niwa, H., Gene. 108 (2), 193-200 (1991))。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08938 pCAGGS DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08938z.seq
            Remarks, protocol and/or map (gif) RDB08938a.gif

            Original reference

            original Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08938_A4Afp1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CAG_R1
            Region: CAG pro,beta-globin p(A)
            Sequence file: RDB08938_A4Afc.seq check
            >08938_08938_A9D4_CAG_R1_beta_F09_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNCTCNAGCG GGNGCGGGGC GAANCGGTGC GGCGCCGGCA
               61 GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC
              121 TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG
              181 GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT
              241 TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG
              301 GCAAAGAATT CCTCGAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG
              361 CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC TCTGCCAAAA
              421 ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA
              481 TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA
              541 GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGCCC
              601 ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC
              661 CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTNN
              721 NNNNTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC
              781 TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT
              841 NGANNTNCCT CNANCTGCAN CCNANGCTTG GCGTAATCAT GGNCNTAGCT GTTNNCTGTG
              901 TGNAANNTNT TATCCGCTCN CNNTTNCNCN NNACANNCNN NNNCNGAANC ANNAANTNNN
            961 ANNNCNNGGG GGNGNCNAAN NGANNNNNNC NNANNNNNNN NNN
            //
            Primer: SV40pA3'element Reverse
            Region: SV40p(A),SV40 pro_ori
            Sequence file: RDB08938_A4Afd.seq check
            >08938_08938_A4Af_SV40pA3'Element Reverse_F05_17.ab1
                1 NNNNNNNNNN NNGGNNNNNN NNNNNGNNNN NNGCNNGCAA CGCGGCCTTT TTACGGTTCC
               61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
              121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
              181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
              241 CGCGTTGGCC GATTCATTAA TGCAGCGGAT CCAGACATGA TAAGATACAT TGATGAGTTT
              301 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT
              361 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAGCTTTTT GCAAAAGCCT
              421 AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG
              481 GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC
              541 GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA
              601 TGCGGATCCG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA
              661 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC
              721 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACNNTG
              781 ATTACGCCAA GCTTGGGCTG CAGGTCGANG GATCTCCNTA AGAGAAGANG GACNGCTATG
              841 ACTGGNNNTA GTCAGGANAN GAGGAAAAAT CTGGCTAGTA AANNATGTNA GGAAAATTTT
              901 AGGNNNGTTA AAGAAAAAAA TAACNCAAAN CAAAANATAA AAAAAATCTA ACCTCAAGTC
              961 NANGGCTTTT CTANNGNAAN ANNNAATGGN NAGNNNGGGG GNNNNNTTNN NTANNCTNNN
            1021 GANCNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
            user_report Senoo, A., Orthogonal Activation of Metabotropic Glutamate Receptor Using Coordination Chemogenetics. Front. Chem. 9: 825669 (2022). PMID 35096780.
            user_report Yamanaka, S., Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF. EMBO J. 40 (4): e105375 (2021). PMID 33470442.
            user_report Wibisono, H., Tracing location by applying Emerald luciferase in an early phase of murine endometriotic lesion formation. Exp Anim. 2021 Nov 25. doi: 10.1538/expanim.21-0146. Epub ahead of print. PMID 34819403.
            user_report Yamanashi, T., Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination. Sci. Rep. 9 (1): 7863 (2019). PMID 31133660.
            user_report Ide, M., Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. EMBO Mol. Med. 28: e10695 (2019). PMID 31657521.
            user_report Ueda, Y., O-GlcNAcylation-mediated degradation of FBXL2 stabilizes FOXM1 to induce cancer progression. Biochem. Biophys. Res. Commun. pii: S0006-291X (19) 32074-1 (2019). PMID 31679690.
            user_report Nihongaki, Y., CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat. Methods 14 (10): 963-966 (2017). PMID 28892089.
            user_report Yahata, N., TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation. Sci. Rep. 7 (1): 15557 (2017). PMID 29138463.
            user_report Yoshimi, K., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405.
            user_report Yamaura, K., Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry. Nat. Chem. Biol. 12 (10): 822-830 (2016). PMID 27526031.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl