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pCAGGS

Mammalian expression vector with CAG promoter.

Catalog number RDB08938
Resource name pCAGGS
Alternative name stock no 36
Clone info. Mammalian expression vector with CAG promoter.
EcoRI and XhoI sites are available for cloning cDNA.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Niwa, H., Gene. 108 (2), 193-199 (1991)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08938 pCAGGS DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08938z.seq from Depositor
Electronic file Map RDB08938a.gif from Depositor

Original reference

original Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08938_A4Af.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08938_A4Afa.seq
>08938_08938_A9D4_CAG_R1_beta_F09_06_ABI08.ab1
    1 CTGGGAAACG CCGCCGCACC CCCTCTAGCG GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA
   61 GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC
  121 TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG
  181 GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT
  241 TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG
  301 GCAAAGAATT CCTCGAGGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG
  361 CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC TCTGCCAAAA
  421 ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA
  481 TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA
  541 GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGCCC
  601 ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC
  661 CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTTA
  721 TATTTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC
  781 TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT
  841 GGAGATCCCT CGACCTGCAG CCCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
  901 TGAAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GGCCGGAAGC ATAAAGTGTT
  961 AAGCCCTGGG GGTGCCTAAA TGAGTGGAGC TAAACTCAAC ATTAATTGGC GGTTGGCGCT
 1021 CACTGCCCGG CCTTTCCAGT CGGGAAAACC CTGTCCGTTG CCAGCGATCC CGCATTCTCT
 1081 CAATAAGTCA AGCAAACCAT AAGTCCCGGC CCTTACTCGC CCAATCCGGC CCCTAACTCC
1141 CGCGCCCAGA ATCTCGCGCC CCATATGTCG GTTGC
//
Primer: SV40pA3'Element Reverse RDB08938_A4Afb.seq
>08938_08938_A4Af_SV40pA3.E.R._F05_17.ab1
    1 GTGCCCGGGG GGGGCGGGAG CCTATGGAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
   61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
  121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
  181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
  241 CGCGTTGGCC GATTCATTAA TGCAGCGGAT CCAGACATGA TAAGATACAT TGATGAGTTT
  301 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT
  361 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAGCTTTTT GCAAAAGCCT
  421 AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG
  481 GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC
  541 GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA
  601 TGCGGATCCG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA
  661 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC
  721 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG
  781 ATTACGCCAA GCTTGGGCTG CAGGTCGAGG GATCTCCATA AGAGAAGAGG GACAGCTATG
  841 ACTGGGAGTA GTCAGGAGAG GAGGAAAAAT CTGGCTAGTA AAACATGTAA GGAAAATTTT
  901 AGGGATGTTA AAGAAAAAAA TAACACAAAA CAAAATATAA AAAAAATCTA ACCTCAAGTC
  961 AAGGGCTTTT CTATGGGAAT AAGGAATGGA CAGCAGGGGG GCCTGTTTTC ATATACTGAT
 1021 GACCTTCTTT AATAGGCCCA ACCTTTGTTT CATGGCAGCC CAGCATAATG GGCATAATGT
 1081 GCCAAACTCT AAACCAAAAT AACTCATCTG ATGGTTTTAA TTGAATTGGC CCTCCCAATA
1141 ATGGTCCTTT CCCGAAAGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-199 (1991). PMID 1660837.
user report Nihongaki, Y., CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat. Methods 14 (10): 963-966 (2017). PMID 28892089.
user report Yahata, N., TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation. Sci. Rep. 7 (1): 15557 (2017). PMID 29138463.
user report Yoshimi, K., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405.
user report Yamaura, K., Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry. Nat. Chem. Biol. 12 (10): 822-830 (2016). PMID 27526031.

2019.06.12

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