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pCAGGS

Mammalian expression vector with CAG promoter.

Catalog number RDB08938
Resource name pCAGGS
Alternative name stock no 36
Clone info. Mammalian expression vector with CAG promoter.
Comment EcoRI and XhoI sites are available for cloning cDNA.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Depositor|Developer Niwa, Hitoshi |
 
Sequence (full) RDB08938hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB08938_A4Afp1-5.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Niwa, H., Gene. 108 (2), 193-200 (1991)).
提供案内 (日本国内) [open/close]

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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Niwa, H., Gene. 108 (2), 193-200 (1991))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08938 pCAGGS DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAGGS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08938).

Reference section:

Niwa, H., Yamamura, K., Miyazaki, J., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report Taira, J., Computer‐Assisted Identification of Inhibitor with Novel Pharmacophore Targeting First Kringle Domain of Hepatocyte Growth Factor. Chemistry Select 8: e202301577 (2023). [link to RRC of NBRP]
user_report Wibisono, H., Tracing location by applying Emerald luciferase in an early phase of murine endometriotic lesion formation. Exp. Anim. 71 (2): 184-192 (2022). PMID 34819403. [link to RRC of NBRP]
user_report Senoo, A., Orthogonal Activation of Metabotropic Glutamate Receptor Using Coordination Chemogenetics. Front. Chem. 9: 825669 (2022). PMID 35096780. [link to RRC of NBRP]
user_report Yamanaka, S., Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF. EMBO J. 40 (4): e105375 (2021). PMID 33470442. [link to RRC of NBRP]
user_report Yamanashi, T., Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination. Sci. Rep. 9 (1): 7863 (2019). PMID 31133660. [link to RRC of NBRP]
user_report Ide, M., Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. EMBO Mol. Med. 28: e10695 (2019). PMID 31657521. [link to RRC of NBRP]
user_report Ueda, Y., O-GlcNAcylation-mediated degradation of FBXL2 stabilizes FOXM1 to induce cancer progression. Biochem. Biophys. Res. Commun. pii: S0006-291X (19) 32074-1 (2019). PMID 31679690. [link to RRC of NBRP]
user_report Nihongaki, Y., CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat. Methods 14 (10): 963-966 (2017). PMID 28892089. [link to RRC of NBRP]
user_report Yahata, N., TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation. Sci. Rep. 7 (1): 15557 (2017). PMID 29138463. [link to RRC of NBRP]
user_report Yoshimi, K., ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat. Commun. 7: 10431 (2016). PMID 26786405. [link to RRC of NBRP]
user_report Yamaura, K., Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry. Nat. Chem. Biol. 12 (10): 822-830 (2016). PMID 27526031. [link to RRC of NBRP]