Resource data sheet
Prev. |  Lentiviral vector plasmid > 

CSIV-UbC-MCS-IRES2-Venus

Backbone vector of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.

Catalog number RDB08930
Resource name CSIV-UbC-MCS-IRES2-Venus
Clone info. Backbone vector of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-UbC-MCS-IRES2-Venus (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08930 CSIV-UbC-MCS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08930z.seq from Depositor
Electronic file PDF (map, protocol and/or remarks) RDB08930.pdf from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08930_A5C9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F | Sequence: RDB08930_A5C9a.seq
>08930_08930_A5C9_IRES-F_D12_12.ab1
    1 CAGTCTTCTG TGTTTAGTCG AGGTTAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG
   61 GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA AGGGCGAGGA
  121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
  181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT
  241 GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA
  301 CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
  361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
  421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
  541 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
  601 GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
  661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC
  721 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
  781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA GAATTCAAGG CCTCTCGAGC
  841 CTCTAGAGTT AACATCGAGG GATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT
  901 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT
  961 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCCTCCCT
 1021 TGTATAAAAT CCCTGATTGC TGTCTCTTTA TGAAGGAGTT GTGCCCCGTT GTCAGCAACG
 1081 GTGGCGTGGC TGTGCACTGT GATTGCTGAA CGCAACCCCC CCCACTGGTA TTGGGGGGCC
1141 AATTGC
//
Primer: IRES-R | Sequence: RDB08930_A5C9b.seq
>08930_08930_A5C9_IRES-R_E12_15.ab1
    1 GCACAGCCGG CTCGGCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCGCGGCC
   61 GCTGCAGAAT TCTCGAGACC GGTAGCGCTA AACACGGTCC GGTCTAACAA AAAAGCCAAA
  121 AACGGCCAGA ATTTAGCGGA CAATTTACTA GTCTAACACT GAAAATTACA TATTGACCCA
  181 AATGATTACA TTTCAAAAGG TGCCTAAAAA ACTTCACAAA ACACACTCGC CAACCCCGAG
  241 CGCATAGTTC AAAACCGGAG CTTCAGCTAC TTAAGAAGAT AGGTACATAA AACCGACCAA
  301 AGAAACTGAC GCCTCACTTA TCCCTCCCCT CACCAGAGGT CCGGCGCCTG TCGATTCAGG
  361 AGAGCCTACC CTAGGCCCGA ACCCTGCGTC CTGCGACGGA GAAAAGCCTA CCGCACACCT
  421 ACCGGCAGGT GGCCCCACCC TGCATTATAA GCCAACAGAA CGGGTGACGT CACGACACGA
  481 CGAGGGCGCG CGCTCCCAAA GGTACGGGTG CACTGCCCAA CGGCACCGCC ATAACTGCCG
  541 CCCCCGCAAC AGACGACAAA CCGAGTTCTC CAGTCAGTGA CAAACTTCAC GTCAGGGTCC
  601 CCAGATGGTG CCCCAGCCCA TCTCACCCGA ATAAGAGCTT TCCCGCATTA GCGAAGGCCT
  661 CAAGACCTTG GGTTCTTGCC GCCCACCATG CCCCCCACCT TGTTTCAACG ACCTCACAGC
  721 CCGCCTCACA AGCGTCTTCC ATTCAAGACT CGGGAACAGC CGCCATTTTG CTGCGCTCCC
  781 CCCAACCCCC AGTTCAGGGC AACCTTGCTC GCGGACCCAG ACTACAGCCC TTGGCGGTCT
  841 CTCCACACGC TTCCGTCCCA CCGAGCGGCC CGGCGGCCAC GAAAGCCCCG GCCAGCCCAG
  901 CAGCCCGCTA CTCACCAAGT GACGATCACA GCGATCCACA AACAAGAACC GCGACCCAAA
  961 TCCCGGCTGC GACGGAACTA GCTGTGCCAC ACCCGGCGCG TCCTTTATAT AATCATCGGC
 1021 GTTCACCGCC CCACGGGAGA TCCTCGCAGA ATCGCCGAAG AAGGACTACT TTTCTTCGCT
 1081 GTTCCGCCTC TCTGAAGAAA ACAAGTGACC CTAGAGGTTC ACCCAAGTTC CCGCTCCTTA
1141 AAAATGGTTT CAT
//
Primer: CMV-Forward | Sequence: RDB08930_A5C9c.seq
>08930_08930_A5C9_CMV-Forward_F12_18.ab1
    1 CGTCCTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
  121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
  181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
  241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA
  301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG
  361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG
  421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA
  481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA
  541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG
  601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA
  661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA
  721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT
  781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCAT TAGAGTAGCA CCCACCAAGG CAAAGAAGAA GAGTGTGCAG
  901 AGAGAAAAAG AGCAGTGGGA TAGGAGCTTT GTTCCTTGGG TTCTTGGGGA GCAGCAGAGC
  961 ACTATGGGGC GCAGCGTCAT GACGCTGACG TACAGCAGAC ATTATTGTCT GCTATATGCA
 1021 GCAGCCGACC AATTTGCTGA AGGCCTAATT GAGGCCAACG CATCTGATGC ACCTCAGTCT
1081 GGGCAATCAC GCAGGCTCTC TGA
//
Primer: BGH_rev | Sequence: RDB08930_A5C9d.seq
>08930_08930_A5C9_BGHrev_G12_21.ab1
    1 GATTCGGGAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
   61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  181 CAGAGAGACC CAGTACAGTC CGGAATAACT TCGTATAATG TATGCTATAC GAAGTTATGA
  241 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT
  301 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG
  361 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG
  421 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG
  481 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
  541 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
  601 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
  661 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC
  721 AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGAAAGCGA
  781 AAGTCCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA ACCAGTGGGG GTTGCGTCAG
  841 CAAACACAGT GCACACCACG CCACGTTGCC TGACAACGGG CCACAACTCC TCATAAAGAG
  901 ACAGCAACCA GGATTTATAC AAAGAGGAGA AAATGAAAGC CATACGGGAA GCAATAGCAT
  961 GATACAAAGG CATTAAAGCA GCGTATCCAC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA
 1021 TACCAGTTCA ATCTTTCACA AATTTTGTAA TCCAGAGGTG GATTATCGAT AAGCTTTGAT
 1081 CCCCTCGATG TTACTCTAGA GCTCGAGAAG GCCTGATTCC TTACCTGTAC AGCTTCGTCC
1141 AATGCCCGAA GAAGTGAATT CCCCTAGACC GGACGGATA
//
Primer: SV40pro_F | Sequence: RDB08930_A5C9e.seq
>08930_08930_A5C9_SV40pro_F_H12_24.ab1
    1 CCTGTCTGAC ATTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT
  121 CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT
  181 TGACCAGTGC CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA
  241 CCGACCGGCT CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG
  301 ACGACGTGAC CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG
  361 CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA
  421 CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC
  481 GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG
  541 ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  601 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT
  661 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
  721 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC
  781 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT
  841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
  901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
  961 TTTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCC AGCTGCATTA
 1021 ATGATCGGCC CAACGCGCGG GGAGAGCGGT TTGCGTATTG GGGCGCTCTT CGCCTTTCTC
 1081 GCCTCACTGA CTCCGCTTGC GCTTCGCTTC GCTTCCGGCC TGGCGGCGAG CGGGTAATTC
1141 CAGGCCTTCC AACCTCTCGA AAAGGC
//
Primer: UbC-N_R | Sequence: RDB08930_A5C9f.seq
>08930_08930_A9Cq_UbC-N_R_C01_07_ABI24.ab1
    1 CATTAACAGC GGGGCCGCTG TCTGAGCGTC CGGGCGGAAG GATCAGGACG CTCGCTGCGC
   61 CCTTCGTCTG ACGTGGCAGC GCTCGCCGTG AGGAGGGGGG CGCCCGCGGG GGGCGCCAAA
  121 ACCCGGCGCG GAGGCCAGAT CTCCCGATCT CGAGCTAGAA TTCAAAGTGA ATTTCTGCTG
  181 TCCCTGTAAT AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT
  241 TGTATGTCTG TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC
  301 CCTTTTCTTT TAAAATTGTG GATGAATACT GCCATTTGTA GATCCGTTCA CTAATCGAAT
  361 GGATCTGTCT CTGTCTCTCT CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC
  421 CCTCGGGGTT GGGAGGTGGG TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT
  481 CACTATAGAA AGTACAGCAA AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT
  541 GAATAATTTT ATATACCACA GCCAATTTGT TATGTTAAAC CAATTCCACA AACTTGCCCA
  601 TTTATCTAAT TCCAATAATT CTTGTTCATT CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT
  661 TAAGGAGTGT ATTAAGCTTG TGTAATTGTT AATTTCTCTG TCCCACTCCA TCCAGGTCGT
  721 GTGATTCCAA ATCTGTTCCA GAGATTTATT ACTCCAACTA GCATTCCAAG GCACAGCAGT
  781 GGTGCAAATG AGTTTTCCAG AGCAACCCCA AATCCCCAGG AGCTGTTGAT CCTTTAGGTA
  841 TCTTTCCACA GCCAGGATTC TTGCCTGGAG CTGCTTGATG CCCCAGACTG TGAGTTGCAA
  901 CAGATGCTGT TGCGCCTCAA TAGCCCTCAG CAAATTGTTC TGCTGCTGCA CTATACCAGA
  961 CAATAAATTG TCTGGCCCTG TACCGTCAGC GTCATTTGAC GCTGCGCCCA TAGTGCTTTC
 1021 CTGCTGCTCC CAAGGACCAA GGAAACAAGC TCCTATTTCC CACTGCTTCT TTTTCTCTCT
1081 GCACCACCTC GTCTCTTGCC TGATGGTTGC CTACCTCCTA TGGATTCAAG ATTTTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.08.11

GNP_filter3_RDBDEP_html_190809.pl