Sequencing and PCR primers for primer information.
Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.
| Catalog number | RDB08532 |
|---|---|
| Resource name | pMT-PURO |
| Clone info. | Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable. |
| Vector backbone | pMT-PURO (Plasmid) |
| Size of vector backbone | 5.0 kb |
| Selectable markers | Amp^r |
| Depositor | Iwaki, Takayuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology 57(1), 45-49, 2008). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08532 | pMT-PURO | DNA solution |
Electronic file
| Electronic file | Sequence RDB08532z.seq from Depositor |
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Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08532_A4Kk.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB08532_A4Kka.seq |
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>08532_08532_A4Kk_M13(-40)_C07_07.ab1 1 CCGGCGGGAT TATTCGTTGC AGGACAGGAT GTGGTGCCCG ATGTGACTAG CTCTTTGCTG 61 CAGGCCGTCC TATCCTCTGG TTCCGATAAG AGACCCAGAA CTCCGGCCCC CCACCGCCCA 121 CCGCCACCCC CATACATATG TGGTACGCAA GTAAGAGTGC CTGCGCATGC CCCATGTGCC 181 CCACCAAGAG CTTTGCATCC CATACAAGTC CCCAAAGTGG AGAACCGAAC CAATTCTTCG 241 CGGGCAGAAC AAAAGCTTCT GCACACGTCT CCACTCGAAT TTGGAGCCGG CCGGCGTGTG 301 CAAAAGAGGT GAATCGAACG AAAGACCCGT GTGTAAAGCC GCGTTTCCAA AATGTATAAA 361 ACCGAGAGCA TCTGGCCAAT GTGCATCAGT TGTGGTCAGC AGCAAAATCA AGTGAATCAT 421 CTCAGTGCAA CTAAAGGGGG GATCCGATCT CAATATGAAG TTATGCATAT TACTGGCCGT 481 CGTGGCCTTT GTTGGCCTCT CGCTCGGGAG ATCTTCGCGA ACTAGTCCGG ATATCCACGT 541 GTGTACACCT AGGCTTAAGT CTAGAGGGCC CTTCGAAGGT AAGCCTATCC CTAACCCTCT 601 CCTCGGTCTC GATTCTACGC GTACCGGTCA TCATCACCAT CACCATTGAG TTTAAACCCG 661 CTGATCAGCC TCGACTGTGC CTTCTAAGGC CTGAGCTCGC TGATCAGCCT CGATCGAGGA 721 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 781 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 841 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT 901 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA 961 GTCGACCTGC AGGCATGCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 1021 TTTGTTATCC CGCTCACAAT TTCCACACAA CATACGAGCC GGGAAGCATA AAGTGTAAAG 1081 CCCTGGGTGC TAATGAGTGA GCTAACTCAC ATTATTTGCG TGCGCTCACT GACCGCTTTC 1141 CAGTCGGGAA AAACCTGTAT CGGGTTGCTA GC // |
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| Primer: Reverse2 | RDB08532_A4Kkb.seq |
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>08532_08532_A4Kk_Reverse2_D07_10.ab1 1 CATTAAATGA TTCGCCAAGC TTGCATGCCT GCAGGTCGAC TGATCATCAC ATCAGCCATA 61 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA 121 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 181 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 241 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCTCGAT CGAGGCTGAT 301 CAGCGAGCTC AGGCCTTAGA AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACTCAATGG 361 TGATGGTGAT GATGACCGGT ACGCGTAGAA TCGAGACCGA GGAGAGGGTT AGGGATAGGC 421 TTACCTTCGA AGGGCCCTCT AGACTTAAGC CTAGGTGTAC ACACGTGGAT ATCCGGACTA 481 GTTCGCGAAG ATCTCCCGAG CGAGAGGCCA ACAAAGGCCA CGACGGCCAG TAATATGCAT 541 AACTTCATAT TGAGATCGGA TCCCCCCTTT AGTTGCACTG AGATGATTCA CTTGATTTTG 601 CTGCTGACCA CAACTGATGC ACATTGGCCA GATGCTCTCG GTTTTATACA TTTTGGAAAC 661 GCGGCTTTAC ACACGGGTCT TTCGTTCGAT TCACCTCTTT TGCACACGCC GGCCGGCTCC 721 AAATTCGAGT GGAGACGTGT GCAGAAGCTT TTGTTCTGCC CGCGAAGAAT TGGTTCGGTT 781 CTCCACTTTG GGGACTTGTA TGGGATGCAA AGCTCTTGGT GGGGCACATG GGGCATGCGC 841 AGGCACTCTT ACTTGCGTAC CACATATGTA TGGGGGTGGC GGTGGGCGGT GGGGGGCCGG 901 AGTTCTGGGT CTCTTATCGG AACCAGAGGA TAGGACGGCC TGCAGCAAAG AGCTAGTCAC 961 ATCGGGCACC ACATCCTGTC CTGCAACGAA TTAATTCACT GGGCCGTCGT TTTACACGTC 1021 GTGACTGGGA AACCCTGGCG TTACCCACTT TAAATCGCCT TTGCAGCACA TCCCCCCTTT 1081 CGGCAAGCTG GCCGTATAGC CGAGAGGCCG CCACGATTCG GCCCGCTCCT ACCAGCTTGC 1141 GCAGTCCTTG AATTGGCCGG AAATTG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. |
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| reference | Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017). |
| user report | Védrine, M., Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14. PLoS One 13 (8): e0202664 (2018). PMID 30142177. |
| user report | Niwa, Y., Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol. Biol. Cell 27 (5): 744-756 (2016). PMID 26764097. |
2019.03.08
GNP_filter3_RDBDEP_html_190307.pl