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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMT-PURO

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.

Catalog number RDB08532
Resource name pMT-PURO
Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Depositor|Developer Iwaki, Takayuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB08532zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology 57(1), 45-49, 2008).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cytotechnology 57(1), 45-49, 2008)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB08532 pMT-PURO DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08532_A2Gap1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: HB-NOS_R
            Region: MT pro, BiP signal sequence
            Sequence file: RDB08532_A2Gaa.seq check
            >08532_08532_B1CtF_HB-NOS_R_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT TGGGTNNNGC CNGGGTTTTC CCNGTCACGA CGTTGTAAAA
               61 CGACGGCCAG TGAATTAATT CGTTGCAGGA CAGGATGTGG TGCCCGATGT GACTAGCTCT
              121 TTGCTGCAGG CCGTCCTATC CTCTGGTTCC GATAAGAGAC CCAGAACTCC GGCCCCCCAC
              181 CGCCCACCGC CACCCCCATA CATATGTGGT ACGCAAGTAA GAGTGCCTGC GCATGCCCCA
              241 TGTGCCCCAC CAAGAGCTTT GCATCCCATA CAAGTCCCCA AAGTGGAGAA CCGAACCAAT
              301 TCTTCGCGGG CAGAACAAAA GCTTCTGCAC ACGTCTCCAC TCGAATTTGG AGCCGGCCGG
              361 CGTGTGCAAA AGAGGTGAAT CGAACGAAAG ACCCGTGTGT AAAGCCGCGT TTCCAAAATG
              421 TATAAAACCG AGAGCATCTG GCCAATGTGC ATCAGTTGTG GTCAGCAGCA AAATCAAGTG
              481 AATCATCTCA GTGCAACTAA AGGGGGGATC CGATCTCAAT ATGAAGTTAT GCATATTACT
              541 GGCCGTCGTG GCCTTTGTTG GCCTCTCGCT CGGGAGANCT TCGCGAACTA GTCCGGANAT
              601 CCACGTGTGT ACACCTANGC TTAAGTCTAG AGGGCCNTTC NAANGNAAGC CTATCCCTAA
              661 CCCTCTCCTC NGTCTCGANT CNACNCNNAN CNGTCATCAT CNNNATCNCN ANNNNNTTNN
            721 ANNCNNNNNN NNNN
            //
            Primer: reverse
            Region: SV40 pA, 6xHis, V5 tag, BiP signal sequence, MT pro
            Sequence file: RDB08532_A2Gab.seq check
            >08532_08532_B1CtF_reverse_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNCNNNNNNN NNTAATCNGC CNTACCACAT TTGTAGAGGT TTTACTTGCT
               61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
              121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
              181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
              241 TCTTATCATG TCTGGATCCT CGATCGAGGC TGATCAGCGA GCTCAGGCCT TAGAAGGCAC
              301 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTACGCGT
              361 AGAATCGAGA CCGAGGAGAG GGTTAGGGAT AGGCTTACCT TCGAAGGGCC CTCTAGACTT
              421 AAGCCTAGGT GTACACACGT GGATATCCGG ACTAGTTCGC GAAGATCTCC CGAGCGAGAG
              481 GCCAACAAAG GCCACGACGG CCAGTAATAT GCATAACTTC ATATTGAGAT CGGATCCCCC
              541 CTTTAGTTGC ACTGAGATGA TTCACTTGAT TTTGCTGCTG ACCACAACTG ATGCACATTG
              601 GCCAGATGCT CTCGGTTTTA TACATTTTGG AAACNCGGCT TTACACACGG GTCTTNCGTT
            661 CNANTNNCCT CNTTNNNANN CNNNNGNNNN GCTNNNAANT NNNNNNNNNN
            //
            Primer: COPIApro-F
            Region: copia pro, PuroR
            Sequence file: RDB08532_A2Gac.seq check
            >08532_08532_B1CtF_COPIApro-F_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCTT TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC
               61 TGGTGTTGCA GTTGAAATAA CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT
              121 ATTTATTTAT TTATTAAGAA AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT
              181 GCAGGAATTC GATAGCTTGC ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT
              241 CCCCTGACCC ACGCCCCTGA CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC
              301 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG
              361 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG
              421 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
              481 TCNNGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC NGANAGCGTC GAAGCGGGGG
              541 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGANTTGAG CGGNTCCNGG CTGGNCGCGC
              601 ANCAACAGAN GGNANGGNCT CCTGGCGCCN CANCNGCCNN ANGNANCCCN NGNNGNNNCN
            661 NGNNNNNNGN NNNNNTCNNN NNNNNNN
            //
            Primer: Amp_R
            Region: AmpR pro, SV40 pA
            Sequence file: RDB08532_A2Gad.seq check
            >08532_08532_B1CtF_Amp_R_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNTNANCAT TTATNNGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
               61 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
              121 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG
              181 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG
              241 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG
              301 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA
              361 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
              421 ATCAGGCGCC GGTACCCGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA
              481 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
              541 TTGTAACCAT TATAAGCTGC AATAAACAAG TTCAAGCTAG CTGGCCTCGA NGTCGACGGT
              601 ATCGATAAGC TTGATCTAGA GTCNGTGGGC CTCGGGGGCG GGNGCGGGGG NCGGCGGGGC
              661 CGCCNNNGGN NGNTTNNNNC NNNNCCNTGG GGNCNNGNGC TNNNTTNNGN NNNGGNNNNN
            721 NNGNNNNNNG GGGNNNGGNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171.
            reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017).
            user_report Védrine, M., Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14. PLoS One 13 (8): e0202664 (2018). PMID 30142177.
            user_report Niwa, Y., Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol. Biol. Cell 27 (5): 744-756 (2016). PMID 26764097.

            2022.08.12

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