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pMT-PURO

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.

Catalog number RDB08532
Resource name pMT-PURO
Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Depositor Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology 57(1), 45-49, 2008).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08532 pMT-PURO DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08532z.seq from Depositor

Featured content

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References

original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08532_A4Kk.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB08532_A4Kka.seq
>08532_08532_A4Kk_M13(-40)_C07_07.ab1
    1 CCGGCGGGAT TATTCGTTGC AGGACAGGAT GTGGTGCCCG ATGTGACTAG CTCTTTGCTG
   61 CAGGCCGTCC TATCCTCTGG TTCCGATAAG AGACCCAGAA CTCCGGCCCC CCACCGCCCA
  121 CCGCCACCCC CATACATATG TGGTACGCAA GTAAGAGTGC CTGCGCATGC CCCATGTGCC
  181 CCACCAAGAG CTTTGCATCC CATACAAGTC CCCAAAGTGG AGAACCGAAC CAATTCTTCG
  241 CGGGCAGAAC AAAAGCTTCT GCACACGTCT CCACTCGAAT TTGGAGCCGG CCGGCGTGTG
  301 CAAAAGAGGT GAATCGAACG AAAGACCCGT GTGTAAAGCC GCGTTTCCAA AATGTATAAA
  361 ACCGAGAGCA TCTGGCCAAT GTGCATCAGT TGTGGTCAGC AGCAAAATCA AGTGAATCAT
  421 CTCAGTGCAA CTAAAGGGGG GATCCGATCT CAATATGAAG TTATGCATAT TACTGGCCGT
  481 CGTGGCCTTT GTTGGCCTCT CGCTCGGGAG ATCTTCGCGA ACTAGTCCGG ATATCCACGT
  541 GTGTACACCT AGGCTTAAGT CTAGAGGGCC CTTCGAAGGT AAGCCTATCC CTAACCCTCT
  601 CCTCGGTCTC GATTCTACGC GTACCGGTCA TCATCACCAT CACCATTGAG TTTAAACCCG
  661 CTGATCAGCC TCGACTGTGC CTTCTAAGGC CTGAGCTCGC TGATCAGCCT CGATCGAGGA
  721 TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
  781 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
  841 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT
  901 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA
  961 GTCGACCTGC AGGCATGCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA
 1021 TTTGTTATCC CGCTCACAAT TTCCACACAA CATACGAGCC GGGAAGCATA AAGTGTAAAG
 1081 CCCTGGGTGC TAATGAGTGA GCTAACTCAC ATTATTTGCG TGCGCTCACT GACCGCTTTC
1141 CAGTCGGGAA AAACCTGTAT CGGGTTGCTA GC
//
Primer: Reverse2 RDB08532_A4Kkb.seq
>08532_08532_A4Kk_Reverse2_D07_10.ab1
    1 CATTAAATGA TTCGCCAAGC TTGCATGCCT GCAGGTCGAC TGATCATCAC ATCAGCCATA
   61 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
  121 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
  181 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
  241 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCTCGAT CGAGGCTGAT
  301 CAGCGAGCTC AGGCCTTAGA AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACTCAATGG
  361 TGATGGTGAT GATGACCGGT ACGCGTAGAA TCGAGACCGA GGAGAGGGTT AGGGATAGGC
  421 TTACCTTCGA AGGGCCCTCT AGACTTAAGC CTAGGTGTAC ACACGTGGAT ATCCGGACTA
  481 GTTCGCGAAG ATCTCCCGAG CGAGAGGCCA ACAAAGGCCA CGACGGCCAG TAATATGCAT
  541 AACTTCATAT TGAGATCGGA TCCCCCCTTT AGTTGCACTG AGATGATTCA CTTGATTTTG
  601 CTGCTGACCA CAACTGATGC ACATTGGCCA GATGCTCTCG GTTTTATACA TTTTGGAAAC
  661 GCGGCTTTAC ACACGGGTCT TTCGTTCGAT TCACCTCTTT TGCACACGCC GGCCGGCTCC
  721 AAATTCGAGT GGAGACGTGT GCAGAAGCTT TTGTTCTGCC CGCGAAGAAT TGGTTCGGTT
  781 CTCCACTTTG GGGACTTGTA TGGGATGCAA AGCTCTTGGT GGGGCACATG GGGCATGCGC
  841 AGGCACTCTT ACTTGCGTAC CACATATGTA TGGGGGTGGC GGTGGGCGGT GGGGGGCCGG
  901 AGTTCTGGGT CTCTTATCGG AACCAGAGGA TAGGACGGCC TGCAGCAAAG AGCTAGTCAC
  961 ATCGGGCACC ACATCCTGTC CTGCAACGAA TTAATTCACT GGGCCGTCGT TTTACACGTC
 1021 GTGACTGGGA AACCCTGGCG TTACCCACTT TAAATCGCCT TTGCAGCACA TCCCCCCTTT
 1081 CGGCAAGCTG GCCGTATAGC CGAGAGGCCG CCACGATTCG GCCCGCTCCT ACCAGCTTGC
1141 GCAGTCCTTG AATTGGCCGG AAATTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171.
reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017).
user report Védrine, M., Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14. PLoS One 13 (8): e0202664 (2018). PMID 30142177.
user report Niwa, Y., Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol. Biol. Cell 27 (5): 744-756 (2016). PMID 26764097.

2019.03.08

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