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pCoPURO

Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.

Catalog number RDB08531
Resource name pCoPURO
Clone info. Copia promoter expression vector of puromycin resistance gene for Schneider 2 (S2) Drosophila cells.
Vector backbone pCoPURO (Plasmid)
Size of vector backbone 4,040 kb
Selectable markers Amp^r
Depositor Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Biotechniques 35(3), 482-484, 486, 2003).
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RDB08531 pCoPURO DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08531z.seq from Depositor

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References

original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08531_A3Eop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB08531_A3Eoa.seq
>08531_08531_A3Eo_M13_-40_C04_07.ab1
    1 AAGCAGGGCC AGCTTGCATG CCTGCAGGTC GACTCTAGAG GATCCGGTGC GTGGTGGTTT
   61 CATGCTTCTG GGAACGGCAA ATGGGTTTAG GATTGGGAAC CCCTCATCAT CTGTTGGAAT
  121 ATACTATTCA ACCTACAAAA GTAACGTTAA ACAACACTAC TTTATATTTG ATATGAATGG
  181 CCACACCTTT TATGCCATAA AACATATTGT AAGAGAATAC CACTCTTTTT ATTCCTTCTT
  241 TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC TGGTGTTGCA GTTGAAATAA
  301 CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT ATTTATTTAT TTATTAAGAA
  361 AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT GCAGGAATTC GATAGCTTGC
  421 ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT CCCCTGACCC ACGCCCCTGA
  481 CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC
  541 CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC
  601 ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC
  661 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG
  721 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC
  781 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC
  841 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC
  901 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG
  961 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG
 1021 CGGCTCGGGC TTCACCGTCA CCGCCCGACG TCGAGGTGCC CGAGACGCGC ACTGATGCAT
 1081 GACCGGCAGC CGGTGCTGAC GCCCGCCCAC GACGCACGTC TGACCGAAGA GCGCACGGAC
1141 CCCATGGCTC CGAACCGGAA GCTAT
//
Primer: Reverse2 RDB08531_A3Eob.seq
>08531_08531_A3Eo_Reverse2_D04_10.ab1
    1 CAGACATGAA TTCGATTCGA GCTCGGTACC CGATCCAGAC ATGATAAGAT ACATTGATGA
   61 GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA
  121 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTCAAG CTAGCTGGCC
  181 TCGAGGTCGA CGGTATCGAT AAGCTTGATC TAGAGTCGGT GGGCCTCGGG GGCGGGTGCG
  241 GGGTCGGCGG GGCCGCCCCG GGTGGCTTCG GTCGGAGCCA TGGGGTCGTG CGCTCCTTTC
  301 GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG TCAGGCACCG GGCTTGCGGG TCATGCACCA
  361 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA
  421 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC
  481 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA
  541 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC
  601 TTCCATCTGT TGCTGCGCGG CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT
  661 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC
  721 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC
  781 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG
  841 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC
  901 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGAAGGTCGT CTCCTTGTGA GGGTCAGGGG
  961 CGTGGGTCAG GGATGGTGGC GGCACCGGGT CGTGGCGGGC CGACCTGCAA GCATGCAAGC
 1021 TATCGAATTT CCTGCAGCCC GGGGGGATCT GTGTATTTAT AATTTTATAT TTTCTTTTCT
 1081 ATAATAAATA AATAGGTCAA GTTTATGTTG AAGTTTTAAT GAATTTATAT TTTAAGTTAT
1141 TTTCAACTGC CAAACCATC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552.
reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017).

2018.12.10

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