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pCoPURO

Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.

Catalog number RDB08531
Resource name pCoPURO
Clone info. Copia promoter expression vector of puromycin resistance gene for Schneider 2 (S2) Drosophila cells.
Vector backbone pCoPURO (Plasmid)
Size of vector backbone 4,040 kb
Selectable markers Amp^r
Depositor|Developer Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Biotechniques 35(3), 482-484, 486, 2003).
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RDB08531 pCoPURO DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB08531zzk02.seq

Original reference

original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0056j (Japanese text)
Featured content Dnaconda's recommendation EXR0056e (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08531_A3Eop1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Region: copia pro,PuroR
Sequence file: RDB08531_A3Eoa.seq check
>08531_08531_A3Eo_M13_-40_C04_07.ab1
    1 AAGCAGGGCC AGCTTGCATG CCTGCAGGTC GACTCTAGAG GATCCGGTGC GTGGTGGTTT
   61 CATGCTTCTG GGAACGGCAA ATGGGTTTAG GATTGGGAAC CCCTCATCAT CTGTTGGAAT
  121 ATACTATTCA ACCTACAAAA GTAACGTTAA ACAACACTAC TTTATATTTG ATATGAATGG
  181 CCACACCTTT TATGCCATAA AACATATTGT AAGAGAATAC CACTCTTTTT ATTCCTTCTT
  241 TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC TGGTGTTGCA GTTGAAATAA
  301 CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT ATTTATTTAT TTATTAAGAA
  361 AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT GCAGGAATTC GATAGCTTGC
  421 ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT CCCCTGACCC ACGCCCCTGA
  481 CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC
  541 CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC
  601 ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC
  661 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG
  721 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC
  781 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC
  841 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC
  901 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG
  961 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG
 1021 CGGCTCGGGC TTCACCGTCA CCGCCCGACG TCGAGGTGCC CGAGACGCGC ACTGATGCAT
 1081 GACCGGCAGC CGGTGCTGAC GCCCGCCCAC GACGCACGTC TGACCGAAGA GCGCACGGAC
1141 CCCATGGCTC CGAACCGGAA GCTAT
//
Primer: reverse-2
Region: SV40pA,PuroR
Sequence file: RDB08531_A3Eob.seq check
>08531_08531_A3Eo_Reverse2_D04_10.ab1
    1 CAGACATGAA TTCGATTCGA GCTCGGTACC CGATCCAGAC ATGATAAGAT ACATTGATGA
   61 GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA
  121 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTCAAG CTAGCTGGCC
  181 TCGAGGTCGA CGGTATCGAT AAGCTTGATC TAGAGTCGGT GGGCCTCGGG GGCGGGTGCG
  241 GGGTCGGCGG GGCCGCCCCG GGTGGCTTCG GTCGGAGCCA TGGGGTCGTG CGCTCCTTTC
  301 GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG TCAGGCACCG GGCTTGCGGG TCATGCACCA
  361 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA
  421 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC
  481 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA
  541 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC
  601 TTCCATCTGT TGCTGCGCGG CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT
  661 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC
  721 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC
  781 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG
  841 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC
  901 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGAAGGTCGT CTCCTTGTGA GGGTCAGGGG
  961 CGTGGGTCAG GGATGGTGGC GGCACCGGGT CGTGGCGGGC CGACCTGCAA GCATGCAAGC
 1021 TATCGAATTT CCTGCAGCCC GGGGGGATCT GTGTATTTAT AATTTTATAT TTTCTTTTCT
 1081 ATAATAAATA AATAGGTCAA GTTTATGTTG AAGTTTTAAT GAATTTATAT TTTAAGTTAT
1141 TTTCAACTGC CAAACCATC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552.
reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017).
reference Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807.
protocol Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171.

2021.02.03

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