Sequencing and PCR primers for primer information.
Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.
| Catalog number | RDB08531 |
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| Resource name | pCoPURO |
| Clone info. | Copia promoter expression vector of puromycin resistance gene for Schneider 2 (S2) Drosophila cells. |
| Vector backbone | pCoPURO (Plasmid) |
| Size of vector backbone | 4,040 kb |
| Selectable markers | Amp^r |
| Depositor | Iwaki, Takayuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Biotechniques 35(3), 482-484, 486, 2003). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08531 | pCoPURO | DNA solution |
Electronic file
| Electronic file | Sequence RDB08531z.seq from Depositor |
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Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
References
| original | Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08531_A3Eop1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: M13_-40 | RDB08531_A3Eoa.seq |
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>08531_08531_A3Eo_M13_-40_C04_07.ab1 1 AAGCAGGGCC AGCTTGCATG CCTGCAGGTC GACTCTAGAG GATCCGGTGC GTGGTGGTTT 61 CATGCTTCTG GGAACGGCAA ATGGGTTTAG GATTGGGAAC CCCTCATCAT CTGTTGGAAT 121 ATACTATTCA ACCTACAAAA GTAACGTTAA ACAACACTAC TTTATATTTG ATATGAATGG 181 CCACACCTTT TATGCCATAA AACATATTGT AAGAGAATAC CACTCTTTTT ATTCCTTCTT 241 TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC TGGTGTTGCA GTTGAAATAA 301 CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT ATTTATTTAT TTATTAAGAA 361 AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT GCAGGAATTC GATAGCTTGC 421 ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT CCCCTGACCC ACGCCCCTGA 481 CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC 541 CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC 601 ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC 661 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG 721 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC 781 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC 841 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC 901 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG 961 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG 1021 CGGCTCGGGC TTCACCGTCA CCGCCCGACG TCGAGGTGCC CGAGACGCGC ACTGATGCAT 1081 GACCGGCAGC CGGTGCTGAC GCCCGCCCAC GACGCACGTC TGACCGAAGA GCGCACGGAC 1141 CCCATGGCTC CGAACCGGAA GCTAT // |
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| Primer: Reverse2 | RDB08531_A3Eob.seq |
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>08531_08531_A3Eo_Reverse2_D04_10.ab1 1 CAGACATGAA TTCGATTCGA GCTCGGTACC CGATCCAGAC ATGATAAGAT ACATTGATGA 61 GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA 121 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTCAAG CTAGCTGGCC 181 TCGAGGTCGA CGGTATCGAT AAGCTTGATC TAGAGTCGGT GGGCCTCGGG GGCGGGTGCG 241 GGGTCGGCGG GGCCGCCCCG GGTGGCTTCG GTCGGAGCCA TGGGGTCGTG CGCTCCTTTC 301 GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG TCAGGCACCG GGCTTGCGGG TCATGCACCA 361 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA 421 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC 481 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA 541 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC 601 TTCCATCTGT TGCTGCGCGG CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT 661 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC 721 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC 781 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG 841 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC 901 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGAAGGTCGT CTCCTTGTGA GGGTCAGGGG 961 CGTGGGTCAG GGATGGTGGC GGCACCGGGT CGTGGCGGGC CGACCTGCAA GCATGCAAGC 1021 TATCGAATTT CCTGCAGCCC GGGGGGATCT GTGTATTTAT AATTTTATAT TTTCTTTTCT 1081 ATAATAAATA AATAGGTCAA GTTTATGTTG AAGTTTTAAT GAATTTATAT TTTAAGTTAT 1141 TTTCAACTGC CAAACCATC // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552. |
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| reference | Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017). |
2018.12.10
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