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pCoPURO

Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.

Catalog number RDB08531
Resource name pCoPURO
Clone info. Copia promoter expression vector of puromycin resistance gene for Schneider 2 (S2) Drosophila cells.
Vector backbone pCoPURO (Plasmid)
Size of vector backbone 4,040 kb
Selectable markers Amp^r
Depositor|Developer Iwaki, Takayuki |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Biotechniques 35(3), 482-484, 486, 2003).
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biotechniques 35(3), 482-484, 486, 2003)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08531 pCoPURO DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB08531zzk02.seq provided by the depositor

            Original reference

            original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0056e (English text)
            Featured content Dnaconda's recommendation EXR0056j (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08531_A3Eop1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB17487_A3Eoa.seq check
            1
            Primer: reverse-2
            Region: SV40pA,PuroR
            Sequence file: RDB08531_A3Eob.seq check
            >08531_08531_A3Eo_Reverse2_D04_10.ab1
                1 CAGACATGAA TTCGATTCGA GCTCGGTACC CGATCCAGAC ATGATAAGAT ACATTGATGA
               61 GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA
              121 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTCAAG CTAGCTGGCC
              181 TCGAGGTCGA CGGTATCGAT AAGCTTGATC TAGAGTCGGT GGGCCTCGGG GGCGGGTGCG
              241 GGGTCGGCGG GGCCGCCCCG GGTGGCTTCG GTCGGAGCCA TGGGGTCGTG CGCTCCTTTC
              301 GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG TCAGGCACCG GGCTTGCGGG TCATGCACCA
              361 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA
              421 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC
              481 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA
              541 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC
              601 TTCCATCTGT TGCTGCGCGG CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT
              661 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC
              721 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC
              781 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG
              841 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC
              901 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGAAGGTCGT CTCCTTGTGA GGGTCAGGGG
              961 CGTGGGTCAG GGATGGTGGC GGCACCGGGT CGTGGCGGGC CGACCTGCAA GCATGCAAGC
             1021 TATCGAATTT CCTGCAGCCC GGGGGGATCT GTGTATTTAT AATTTTATAT TTTCTTTTCT
             1081 ATAATAAATA AATAGGTCAA GTTTATGTTG AAGTTTTAAT GAATTTATAT TTTAAGTTAT
            1141 TTTCAACTGC CAAACCATC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552.
            reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017).
            reference Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807.
            protocol Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171.

            2022.02.09

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