Resource data sheet
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pCoPURO (#RDB08531)

Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format
SnapGene file
Publication Iwaki, T., Biotechniques 35 (3), 482-486, (2003). PMID 14513552. [link to RRC of NBRP]
Test sheet Data Sheet 
 
Clone info. Copia promoter expression vector of puromycin resistance gene for Schneider 2 (S2) Drosophila cells.
Vector backbone pCoPURO (Plasmid)
Size of vector backbone 4,040 kb
Selectable markers Amp^r
Growth conditions 37C, LB + Amp
Depositor|Developer Iwaki, Takayuki |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Biotechniques 35(3), 482-484, 486, 2003).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biotechniques 35(3), 482-484, 486, 2003)。

Catalog # Resource name Shipping form Fee
RDB08531 pCoPURO DNA solution

How to cite this biological resource

Materials & Methods section:

The pCoPURO was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08531).

Reference section:

Iwaki, T., Figuera, M., Ploplis, V.A., Castellino, F.J., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552. [link to RRC of NBRP]
reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017). [link to Journal]
reference Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807. [link to Journal]
protocol Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. [link to RRC of NBRP]