Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells.
Catalog number | RDB08531 |
---|---|
Resource name | pCoPURO |
Clone info. | Copia promoter expression vector of puromycin resistance gene for Schneider 2 (S2) Drosophila cells. |
Vector backbone | pCoPURO (Plasmid) |
Size of vector backbone | 4,040 kb |
Selectable markers | Amp^r |
Depositor|Developer | Iwaki, Takayuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Biotechniques 35(3), 482-484, 486, 2003). |
---|---|
Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB08531 | pCoPURO | DNA solution |
Electronic file
Electronic file | Nucleotide sequence provided by the depositor RDB08531zzk02.seq |
---|
Original reference
original | Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552. |
---|
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Dnaconda's recommendation EXR0056j (Japanese text) |
---|---|
Featured content | Dnaconda's recommendation EXR0056e (English text) |
Featured content | Empty Backbone (English text) |
Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB08531_A3Eop1-2.pdf ![]() |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 Region: copia pro,PuroR Sequence file: RDB08531_A3Eoa.seq ![]() |
---|
>08531_08531_A3Eo_M13_-40_C04_07.ab1 1 AAGCAGGGCC AGCTTGCATG CCTGCAGGTC GACTCTAGAG GATCCGGTGC GTGGTGGTTT 61 CATGCTTCTG GGAACGGCAA ATGGGTTTAG GATTGGGAAC CCCTCATCAT CTGTTGGAAT 121 ATACTATTCA ACCTACAAAA GTAACGTTAA ACAACACTAC TTTATATTTG ATATGAATGG 181 CCACACCTTT TATGCCATAA AACATATTGT AAGAGAATAC CACTCTTTTT ATTCCTTCTT 241 TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC TGGTGTTGCA GTTGAAATAA 301 CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT ATTTATTTAT TTATTAAGAA 361 AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT GCAGGAATTC GATAGCTTGC 421 ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT CCCCTGACCC ACGCCCCTGA 481 CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC 541 CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC 601 ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC 661 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG 721 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC 781 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC 841 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC 901 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG 961 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG 1021 CGGCTCGGGC TTCACCGTCA CCGCCCGACG TCGAGGTGCC CGAGACGCGC ACTGATGCAT 1081 GACCGGCAGC CGGTGCTGAC GCCCGCCCAC GACGCACGTC TGACCGAAGA GCGCACGGAC 1141 CCCATGGCTC CGAACCGGAA GCTAT // |
Primer: reverse-2 Region: SV40pA,PuroR Sequence file: RDB08531_A3Eob.seq ![]() |
>08531_08531_A3Eo_Reverse2_D04_10.ab1 1 CAGACATGAA TTCGATTCGA GCTCGGTACC CGATCCAGAC ATGATAAGAT ACATTGATGA 61 GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA 121 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTCAAG CTAGCTGGCC 181 TCGAGGTCGA CGGTATCGAT AAGCTTGATC TAGAGTCGGT GGGCCTCGGG GGCGGGTGCG 241 GGGTCGGCGG GGCCGCCCCG GGTGGCTTCG GTCGGAGCCA TGGGGTCGTG CGCTCCTTTC 301 GGTCGGGCGC TGCGGGTCGT GGGGCGGGCG TCAGGCACCG GGCTTGCGGG TCATGCACCA 361 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA 421 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC 481 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA 541 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC 601 TTCCATCTGT TGCTGCGCGG CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT 661 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC 721 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC 781 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG 841 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC 901 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGAAGGTCGT CTCCTTGTGA GGGTCAGGGG 961 CGTGGGTCAG GGATGGTGGC GGCACCGGGT CGTGGCGGGC CGACCTGCAA GCATGCAAGC 1021 TATCGAATTT CCTGCAGCCC GGGGGGATCT GTGTATTTAT AATTTTATAT TTTCTTTTCT 1081 ATAATAAATA AATAGGTCAA GTTTATGTTG AAGTTTTAAT GAATTTATAT TTTAAGTTAT 1141 TTTCAACTGC CAAACCATC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Iwaki, T., Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35 (3), 482-486, (2003). PMID 14513552. |
---|---|
reference | Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017). |
reference | Nagahashi, K., Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69 (2): 317-327 (2017). PMID 28070807. |
protocol | Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. |
2021.02.03
GNP_filter3_RDBDEP_html_201222.pl