Expression clone of GFP under the control of CAG promoter, neomycin resistance
| Catalog number | RDB08530 |
|---|---|
| Resource name | pBRPyCAG-EGFP-IN |
| Alternative name | stock no 10685 |
| Clone info. | Expression clone of GFP under the control of CAG promoter, neomycin resistance. |
| Vector backbone | pBRPyCAG-cHA-IN (Plasmid) |
| Size of vector backbone | 6.7 kb |
| Selectable markers | Amp^r (E. coli); neomycin (mammalian cells) |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08530 | pBRPyCAG-EGFP-IN | DNA solution |
Electronic file
| Electronic file | Sequence RDB08530z.seq from Depositor |
|---|---|
| Electronic file | Map RDB08530a.gif from Depositor
|
Featured content
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08530_A4L1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB08530_A4L1a.seq |
|---|---|
|
>08530_08530_A4L1_pAxCA_F1_A09_03.ab1 1 TGTTGGGGCT TGTTCGCATG CAGGGTCTGC ACAGTTATGC CTTCTTCTTT TTCCTACAGC 61 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGC 121 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT 181 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC 241 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 301 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT 361 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT 421 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC 481 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG 541 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA 601 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT 661 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA 721 CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT 781 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA 841 GTAAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TCCCTCCCCC 901 CCCCTAACGT TACTGGGCCG AAGCCGCTTG GAATAAGGCC GGGTGTGCGT TTGTCTATAT 961 TGTTATTTTT CCACCATATT GCCGTTCTTT GGGCAATGTG AGGGCCCCGG GAAACCTGGG 1021 CCCTGTCTTC TTTGACGGAG CATTCCTTAG GGGGTCTTTT CCTCATCTTC GCCAAGGGAA 1081 TGTGCAAGGG TCTGGTTGAT GGTCGTGAAG GGAAGGCAAG TCTCTGGAAA GCTTTCTTTG 1141 AGACCAACAA CGTTCTGATA GCCGAACCCT TTTGGCACGG CCAACGGCGG GACACGC // |
|
| Primer: IRES-F | RDB08530_A4L1b.seq |
|
>08530_08530_A4L1_IRES-F_B09_06.ab1 1 TATGGGGGGC TGGATGGGGG GGCCCTGCAT CTTTATGTGT TAGTCGAGGT TAAAAAACGT 61 CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTCTTGA AAAACACGAT GATAATATGG 121 CCACAACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 901 GCCTTTCTTG ACGAGTTCTT CTGAGGGGAT CGGCAATAAA AAGACAGAAT AAAACGCACG 961 GGTGTTGGGT CGTTTGTTTC GGATCCCACG GGGTGCGCAT GATCGTGCTT CCTGTCGTTG 1021 AGGACCCGGG CTAGGCTGGC GGGGTTTGCC CTTTACTGGT TTAGCAGAAT GAAATCCACC 1081 GATACCGCCG AGCCGAAACG TGGAAAGCGA CTGCTGCTTG CTAAAACGTC TTGCGACCTG 1141 AGCATCAACC ATTGAAATTG GTCTCGGTTT CGTGTTTTCC CGTAAGATCC TGGTAAAACG 1201 G // |
|
| Primer: SV40pA3'Element Reverse | RDB08530_A4L1c.seq |
|
>08530_08530_A4L1_SV40pA3'ElementReverse_C09_09.ab1 1 AGGCCTCCAG GGGGGGGCCG GGAGCCTATG GAAAACGCCA GCAACGCGGC CTTTTTACGG 61 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT 121 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC 181 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT 241 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT 301 GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC 361 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG 421 CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT 481 CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC 541 AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT 601 GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT 661 CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC 721 TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC 781 AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT 841 TCGTTAATAC AGATGTAGGT GTTCCACAGG GGTAGCCAGC AGCATCCTGC GATGCAGATC 901 CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA 961 CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC 1021 GTTCGCTCGC GTATCGGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GGCCAGCCTA 1081 GCGGGGTTCC TCACCGACCA GGAGCACGAA TCATGCGCAC CGTTGGATCG GACCAACGAA 1141 TCAACAACCG ATGCCGTTAA TTCTGGTCTT TTTTAAATTG // |
|
| Primer: Amp_R | RDB08530_A4L1d.seq |
|
>08530_08530_A4L1_Amp_R_D09_12.ab1 1 TACGGGGGGG GGCAACTCTT TTTATGACCG CTTTGGAAAA CAAAGACTGT ATTTCCCCGC 61 GAAATTAATG TTTATTCAAT AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG 121 AAATGAGGCG GGAAGAGGCG GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG 181 CCTCCAAGGC GCCAGTCCTG TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC 241 CAGAGTAAGC GGAGGCCAGG GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT 301 GTCATAGTAA TGATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT 361 CTTGCAAAAC CACACTGACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA 421 AAACCACACT GCCCTCTGGA GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC 481 GGCCAGCTGC GGTCAGTTAG TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG 541 AGTCGGGAGG AAAATTACTG TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT 601 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 661 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 721 TTATCATGTC TGGATCTGAT ATCATCGTCG ACATTGATTA TTGACTAGCT AGTTATTAAT 781 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC 841 TTACGGTAAA TGGCCCGCCC TGGCTGACCG CCCAACGACC CCCCGCCCAT TGACGTCAAT 901 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGGACTTT TCCATTGACG TCAATGGGTG 961 GAGTATTTTA CGGTAAACTG CCCACTTTGG CAGTACCATC AAAGTGTATC ATATGCCAAA 1021 GTACCGCCCC CCTATTGGAC GTCAATGACG GTAAATGGGC CCGCCCTGGC ATTATGCCCA 1081 GTACATGGAA CCTTAATGGG GACTTTCCTT ACTTGGCCAG TTACAATCTT AGCTTATTAG 1141 TCATTCGCTA TTAACCATGG AATGGGCTCG AGGTGAAGGC CTCCCACGCG TTTCCTGTGC 1201 CTTTACACTT CTTTCCCCCC CAT // |
|
Please visit 
Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl