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pBRPyCAG-EGFP-IN

Expression clone of GFP under the control of CAG promoter, neomycin resistance

Catalog number RDB08530
Resource name pBRPyCAG-EGFP-IN
Alternative name stock no 10685
Clone info. Expression clone of GFP under the control of CAG promoter, neomycin resistance.
Vector backbone pBRPyCAG-cHA-IN (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r (E. coli); neomycin (mammalian cells)
Gene/insert name Aequorea victoria GFP cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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RDB08530 pBRPyCAG-EGFP-IN DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08530z.seq from Depositor
Electronic file Map RDB08530a.gif from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08530_A4L1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08530_A4L1a.seq
>08530_08530_A4L1_pAxCA_F1_A09_03.ab1
    1 TGTTGGGGCT TGTTCGCATG CAGGGTCTGC ACAGTTATGC CTTCTTCTTT TTCCTACAGC
   61 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGC
  121 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
  181 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
  241 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
  301 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
  361 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
  421 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
  481 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
  541 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA
  601 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT
  661 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
  721 CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT
  781 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
  841 GTAAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TCCCTCCCCC
  901 CCCCTAACGT TACTGGGCCG AAGCCGCTTG GAATAAGGCC GGGTGTGCGT TTGTCTATAT
  961 TGTTATTTTT CCACCATATT GCCGTTCTTT GGGCAATGTG AGGGCCCCGG GAAACCTGGG
 1021 CCCTGTCTTC TTTGACGGAG CATTCCTTAG GGGGTCTTTT CCTCATCTTC GCCAAGGGAA
 1081 TGTGCAAGGG TCTGGTTGAT GGTCGTGAAG GGAAGGCAAG TCTCTGGAAA GCTTTCTTTG
1141 AGACCAACAA CGTTCTGATA GCCGAACCCT TTTGGCACGG CCAACGGCGG GACACGC
//
Primer: IRES-F RDB08530_A4L1b.seq
>08530_08530_A4L1_IRES-F_B09_06.ab1
    1 TATGGGGGGC TGGATGGGGG GGCCCTGCAT CTTTATGTGT TAGTCGAGGT TAAAAAACGT
   61 CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTCTTGA AAAACACGAT GATAATATGG
  121 CCACAACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTTCTTG ACGAGTTCTT CTGAGGGGAT CGGCAATAAA AAGACAGAAT AAAACGCACG
  961 GGTGTTGGGT CGTTTGTTTC GGATCCCACG GGGTGCGCAT GATCGTGCTT CCTGTCGTTG
 1021 AGGACCCGGG CTAGGCTGGC GGGGTTTGCC CTTTACTGGT TTAGCAGAAT GAAATCCACC
 1081 GATACCGCCG AGCCGAAACG TGGAAAGCGA CTGCTGCTTG CTAAAACGTC TTGCGACCTG
 1141 AGCATCAACC ATTGAAATTG GTCTCGGTTT CGTGTTTTCC CGTAAGATCC TGGTAAAACG
1201 G
//
Primer: SV40pA3'Element Reverse RDB08530_A4L1c.seq
>08530_08530_A4L1_SV40pA3'ElementReverse_C09_09.ab1
    1 AGGCCTCCAG GGGGGGGCCG GGAGCCTATG GAAAACGCCA GCAACGCGGC CTTTTTACGG
   61 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
  121 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
  181 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT
  241 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT
  301 GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC
  361 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG
  421 CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT
  481 CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC
  541 AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT
  601 GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT
  661 CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC
  721 TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC
  781 AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT
  841 TCGTTAATAC AGATGTAGGT GTTCCACAGG GGTAGCCAGC AGCATCCTGC GATGCAGATC
  901 CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA
  961 CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC
 1021 GTTCGCTCGC GTATCGGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GGCCAGCCTA
 1081 GCGGGGTTCC TCACCGACCA GGAGCACGAA TCATGCGCAC CGTTGGATCG GACCAACGAA
1141 TCAACAACCG ATGCCGTTAA TTCTGGTCTT TTTTAAATTG
//
Primer: Amp_R RDB08530_A4L1d.seq
>08530_08530_A4L1_Amp_R_D09_12.ab1
    1 TACGGGGGGG GGCAACTCTT TTTATGACCG CTTTGGAAAA CAAAGACTGT ATTTCCCCGC
   61 GAAATTAATG TTTATTCAAT AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG
  121 AAATGAGGCG GGAAGAGGCG GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG
  181 CCTCCAAGGC GCCAGTCCTG TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC
  241 CAGAGTAAGC GGAGGCCAGG GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT
  301 GTCATAGTAA TGATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT
  361 CTTGCAAAAC CACACTGACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA
  421 AAACCACACT GCCCTCTGGA GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC
  481 GGCCAGCTGC GGTCAGTTAG TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG
  541 AGTCGGGAGG AAAATTACTG TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT
  601 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
  661 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
  721 TTATCATGTC TGGATCTGAT ATCATCGTCG ACATTGATTA TTGACTAGCT AGTTATTAAT
  781 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
  841 TTACGGTAAA TGGCCCGCCC TGGCTGACCG CCCAACGACC CCCCGCCCAT TGACGTCAAT
  901 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGGACTTT TCCATTGACG TCAATGGGTG
  961 GAGTATTTTA CGGTAAACTG CCCACTTTGG CAGTACCATC AAAGTGTATC ATATGCCAAA
 1021 GTACCGCCCC CCTATTGGAC GTCAATGACG GTAAATGGGC CCGCCCTGGC ATTATGCCCA
 1081 GTACATGGAA CCTTAATGGG GACTTTCCTT ACTTGGCCAG TTACAATCTT AGCTTATTAG
 1141 TCATTCGCTA TTAACCATGG AATGGGCTCG AGGTGAAGGC CTCCCACGCG TTTCCTGTGC
1201 CTTTACACTT CTTTCCCCCC CAT
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.11

GNP_filter3_RDBDEP_html_181012.pl