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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBRPyCAG-EGFP-IN (#RDB08530)

Expression clone of GFP under the control of CAG promoter, neomycin resistance

Alternative name stock no 10685
Clone info. Expression clone of GFP under the control of CAG promoter, neomycin resistance.
Vector backbone pBRPyCAG-cHA-IN (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r (E. coli); neomycin (mammalian cells)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB08530z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08530 pBRPyCAG-EGFP-IN DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBRPyCAG-EGFP-IN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08530).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB20093_B3Iqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAG pro,EGFP 5'
Sequence file: RDB20093_B3Iqa.seq check
>08530_20093_B3Iq_1_CAG_R1_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCCCNNCNNN CGGGCGCGGG GCNNANCGGT GCGGCGCCGG
   61 CAGGNAGGAA ATGGGCGGGG NGGGCCTTCG TGCGTCGCCG CGCCGCCGTC CCCTTCTCCC
  121 TCTCCAGCCT CGGGGCTGTC CGCGGGGGGA CGGCTGCCTT CGGGGGGGAC GGGGCAGGGC
  181 GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC
  241 CTTCTTCTTT TTCCTACAGC TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT
  301 TGGCAAAGAA TTCCTCGAGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
  361 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
  421 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
  481 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
  541 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
  601 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
  661 GTGAAGTTCG AGGGCGACAC CCTGGTGAAN CGCATCGAGC TGAANGGCAT CGACTTCNAG
  721 GAGGANGGCA ACNTCCTGGG GCACAAGCTG GANTACAACT ACAACAGCCA CAACGTCTAT
  781 ATCATGGNCG ACAAGCAGAA NAACGGCATC NNNNTGANCT TCNAGATNCG NCACNNCNTC
  841 GANGANNGCA GCGTGNANCN NNNNNANCAN TANCNNCANA NCANCCCCCA TCGGCGANNN
  901 NNNNNNGCNN CTGCCCNNNN NNNCNNNNAN CNNNANCANC CANNNCNNNC NNNNNNNNNN
//
Primer: EGFP-N_F2 (Pr1228)
Region: EGFP 3',IRES
Sequence file: RDB20093_B3Iqb.seq check
>08530_20093_B3Iq_1_EGFP-N_F2_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNCNTCTTCT TCAGGNNGAN NGCAACTACA AGNCCCGCGC
   61 CGNNGNGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
  121 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT
  181 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA
  241 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA
  301 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA
  361 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
  421 TCTCGGCATG GACGAGCTGT ACAAGTAAGC GGCCGCTCGA TAAGCTTGAT ATCGAATTCC
  481 GCCCCCCCCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA
  541 GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG
  601 AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGNATTC CTAGGGGTCT TTCCCCTCTC
  661 GCCNAAGGAA TGCAANGTCT GTTGAATGTC GTGANNNANN NNNTTCCTCT GGAAGCTTCN
  721 TGAANANNAN CNANNNNNCT GTAGCGACCC NTTGCANGCA NNNGNAANCC CCCNNNCNNG
  781 NNNNNCNNNN NGNCTCTGNN GGCCNNAAAG NCCNNGTGNA NNANNNANNN NNNTGCNNNN
  841 NCGGNNNNNN CCNCNNNNGN CCNNNNNTNN NGNANTTNNN NNNNNTNNNN GNNNNNNANT
901 NNANATGNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: Polyoma ori, SV40 pA
Sequence file: RDB20093_B3Iqc.seq check
>08530_20093_B3Iq_1_Amp_R_H02_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNCTTNN GNNNNNNAGN NTGTANTTCC TGGNANTTAA TGTTTATTCA
   61 ATAAACTGTG NATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG
  121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC
  181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA
  241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG
  301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA
  361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG
  421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT
  481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC
  541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC
  601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  661 ACTGCATTCT ANTTGTGNTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG
  721 ANATCATCGT CGACATTGAT TATTGACTAN CTAGTTATTA ATAGTAATCA ATTACGGNNT
  781 CATTAGTTCA TANCCNATAT ATGGANTTNC GCGTTACATA ACTNACNGNA AATGGCCCGC
  841 CNNGCTGANC GCCCAACNNN CCCCNCCNNT TNNCGTCNAT NNNNNNNNAT GNNNCNATAN
901 NAANNNCNNN TANGNNNNTN NNNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES,NeoR 5'
Sequence file: RDB20093_B3Iqd.seq check
>08530_20093_B3Ig_1_IRES-F_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTNN NTCGNNGTTA NNANAACGTC TAGGCCCCCC NAACCACGGG
   61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA TTGAACAAGA
  121 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
  181 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
  241 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC
  301 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
  361 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC
  421 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
  481 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
  541 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT
  601 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGNTCTCGT
  661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGNNC GCTTTTCTGG
  721 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CAGGACATAN CGTTGGCTAC
  781 CCGTGATATT GCTGAAGANC TTGNCGGCGA ATGGGCTGAN CGCTTCCTCN NGCTTTACNG
  841 NATCGCCGCN NCNGANTCNC ANCGCATCGC CNTCTATCGC CTNNTTGANN ANTNCNNNTG
901 ANGGNNNCNG NNNNTAAAAA NANNGAANNA AANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles