Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-EGFP-IN

Expression clone of GFP under the control of CAG promoter, neomycin resistance

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB08530z.seq
Remarks, protocol and/or map (gif)	RDB08530a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08530_A4L1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08530_A4L1a.seq
>08530_08530_A4L1_pAxCA_F1_A09_03.ab1     1 TGTTGGGGCT TGTTCGCATG CAGGGTCTGC ACAGTTATGC CTTCTTCTTT TTCCTACAGC    61 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGC   121 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT   181 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC   241 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC   301 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT   361 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT   421 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC   481 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG   541 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA   601 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT   661 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA   721 CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT   781 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA   841 GTAAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TCCCTCCCCC   901 CCCCTAACGT TACTGGGCCG AAGCCGCTTG GAATAAGGCC GGGTGTGCGT TTGTCTATAT   961 TGTTATTTTT CCACCATATT GCCGTTCTTT GGGCAATGTG AGGGCCCCGG GAAACCTGGG  1021 CCCTGTCTTC TTTGACGGAG CATTCCTTAG GGGGTCTTTT CCTCATCTTC GCCAAGGGAA  1081 TGTGCAAGGG TCTGGTTGAT GGTCGTGAAG GGAAGGCAAG TCTCTGGAAA GCTTTCTTTG 1141 AGACCAACAA CGTTCTGATA GCCGAACCCT TTTGGCACGG CCAACGGCGG GACACGC //
Primer: IRES-F Sequence file: RDB08530_A4L1b.seq
>08530_08530_A4L1_IRES-F_B09_06.ab1     1 TATGGGGGGC TGGATGGGGG GGCCCTGCAT CTTTATGTGT TAGTCGAGGT TAAAAAACGT    61 CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTCTTGA AAAACACGAT GATAATATGG   121 CCACAACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA   661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG   841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC   901 GCCTTTCTTG ACGAGTTCTT CTGAGGGGAT CGGCAATAAA AAGACAGAAT AAAACGCACG   961 GGTGTTGGGT CGTTTGTTTC GGATCCCACG GGGTGCGCAT GATCGTGCTT CCTGTCGTTG  1021 AGGACCCGGG CTAGGCTGGC GGGGTTTGCC CTTTACTGGT TTAGCAGAAT GAAATCCACC  1081 GATACCGCCG AGCCGAAACG TGGAAAGCGA CTGCTGCTTG CTAAAACGTC TTGCGACCTG  1141 AGCATCAACC ATTGAAATTG GTCTCGGTTT CGTGTTTTCC CGTAAGATCC TGGTAAAACG 1201 G //
Primer: SV40pA3'Element Reverse Sequence file: RDB08530_A4L1c.seq
>08530_08530_A4L1_SV40pA3'ElementReverse_C09_09.ab1     1 AGGCCTCCAG GGGGGGGCCG GGAGCCTATG GAAAACGCCA GCAACGCGGC CTTTTTACGG    61 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT   121 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC   181 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT   241 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT   301 GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC   361 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG   421 CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT   481 CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC   541 AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT   601 GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT   661 CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC   721 TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC   781 AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT   841 TCGTTAATAC AGATGTAGGT GTTCCACAGG GGTAGCCAGC AGCATCCTGC GATGCAGATC   901 CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA   961 CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC  1021 GTTCGCTCGC GTATCGGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GGCCAGCCTA  1081 GCGGGGTTCC TCACCGACCA GGAGCACGAA TCATGCGCAC CGTTGGATCG GACCAACGAA 1141 TCAACAACCG ATGCCGTTAA TTCTGGTCTT TTTTAAATTG //
Primer: Amp_R Sequence file: RDB08530_A4L1d.seq
>08530_08530_A4L1_Amp_R_D09_12.ab1     1 TACGGGGGGG GGCAACTCTT TTTATGACCG CTTTGGAAAA CAAAGACTGT ATTTCCCCGC    61 GAAATTAATG TTTATTCAAT AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG   121 AAATGAGGCG GGAAGAGGCG GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG   181 CCTCCAAGGC GCCAGTCCTG TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC   241 CAGAGTAAGC GGAGGCCAGG GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT   301 GTCATAGTAA TGATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT   361 CTTGCAAAAC CACACTGACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA   421 AAACCACACT GCCCTCTGGA GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC   481 GGCCAGCTGC GGTCAGTTAG TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG   541 AGTCGGGAGG AAAATTACTG TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT   601 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC   661 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC   721 TTATCATGTC TGGATCTGAT ATCATCGTCG ACATTGATTA TTGACTAGCT AGTTATTAAT   781 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC   841 TTACGGTAAA TGGCCCGCCC TGGCTGACCG CCCAACGACC CCCCGCCCAT TGACGTCAAT   901 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGGACTTT TCCATTGACG TCAATGGGTG   961 GAGTATTTTA CGGTAAACTG CCCACTTTGG CAGTACCATC AAAGTGTATC ATATGCCAAA  1021 GTACCGCCCC CCTATTGGAC GTCAATGACG GTAAATGGGC CCGCCCTGGC ATTATGCCCA  1081 GTACATGGAA CCTTAATGGG GACTTTCCTT ACTTGGCCAG TTACAATCTT AGCTTATTAG  1141 TCATTCGCTA TTAACCATGG AATGGGCTCG AGGTGAAGGC CTCCCACGCG TTTCCTGTGC 1201 CTTTACACTT CTTTCCCCCC CAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl