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pBRPyCAG-EGFP-IN

Expression clone of GFP under the control of CAG promoter, neomycin resistance

Catalog number RDB08530
Resource name pBRPyCAG-EGFP-IN
Alternative name stock no 10685
Clone info. Expression clone of GFP under the control of CAG promoter, neomycin resistance.
Vector backbone pBRPyCAG-cHA-IN (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r (E. coli); neomycin (mammalian cells)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08530 pBRPyCAG-EGFP-IN DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08530z.seq
            Remarks, protocol and/or map (gif) RDB08530a.gif

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08530_A4L1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB08530_A4L1a.seq check
            >08530_08530_A4L1_pAxCA_F1_A09_03.ab1
                1 TGTTGGGGCT TGTTCGCATG CAGGGTCTGC ACAGTTATGC CTTCTTCTTT TTCCTACAGC
               61 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGC
              121 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
              181 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
              241 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
              301 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
              361 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
              421 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
              481 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
              541 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA
              601 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT
              661 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
              721 CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT
              781 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
              841 GTAAGCGGCC GCTCGATAAG CTTGATATCG AATTCCGCCC CCCCCCCCTC TCCCTCCCCC
              901 CCCCTAACGT TACTGGGCCG AAGCCGCTTG GAATAAGGCC GGGTGTGCGT TTGTCTATAT
              961 TGTTATTTTT CCACCATATT GCCGTTCTTT GGGCAATGTG AGGGCCCCGG GAAACCTGGG
             1021 CCCTGTCTTC TTTGACGGAG CATTCCTTAG GGGGTCTTTT CCTCATCTTC GCCAAGGGAA
             1081 TGTGCAAGGG TCTGGTTGAT GGTCGTGAAG GGAAGGCAAG TCTCTGGAAA GCTTTCTTTG
            1141 AGACCAACAA CGTTCTGATA GCCGAACCCT TTTGGCACGG CCAACGGCGG GACACGC
            //
            Primer: IRES-F
            Sequence file: RDB08530_A4L1b.seq check
            >08530_08530_A4L1_IRES-F_B09_06.ab1
                1 TATGGGGGGC TGGATGGGGG GGCCCTGCAT CTTTATGTGT TAGTCGAGGT TAAAAAACGT
               61 CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTCTTGA AAAACACGAT GATAATATGG
              121 CCACAACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
              181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
              241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
              301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
              361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
              421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
              481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
              541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
              601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
              661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
              721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
              781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
              841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
              901 GCCTTTCTTG ACGAGTTCTT CTGAGGGGAT CGGCAATAAA AAGACAGAAT AAAACGCACG
              961 GGTGTTGGGT CGTTTGTTTC GGATCCCACG GGGTGCGCAT GATCGTGCTT CCTGTCGTTG
             1021 AGGACCCGGG CTAGGCTGGC GGGGTTTGCC CTTTACTGGT TTAGCAGAAT GAAATCCACC
             1081 GATACCGCCG AGCCGAAACG TGGAAAGCGA CTGCTGCTTG CTAAAACGTC TTGCGACCTG
             1141 AGCATCAACC ATTGAAATTG GTCTCGGTTT CGTGTTTTCC CGTAAGATCC TGGTAAAACG
            1201 G
            //
            Primer: SV40pA3'Element Reverse
            Sequence file: RDB08530_A4L1c.seq check
            >08530_08530_A4L1_SV40pA3'ElementReverse_C09_09.ab1
                1 AGGCCTCCAG GGGGGGGCCG GGAGCCTATG GAAAACGCCA GCAACGCGGC CTTTTTACGG
               61 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
              121 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
              181 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT
              241 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT
              301 GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC
              361 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG
              421 CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT
              481 CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC
              541 AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT
              601 GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT
              661 CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC
              721 TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC
              781 AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT
              841 TCGTTAATAC AGATGTAGGT GTTCCACAGG GGTAGCCAGC AGCATCCTGC GATGCAGATC
              901 CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA
              961 CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC
             1021 GTTCGCTCGC GTATCGGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GGCCAGCCTA
             1081 GCGGGGTTCC TCACCGACCA GGAGCACGAA TCATGCGCAC CGTTGGATCG GACCAACGAA
            1141 TCAACAACCG ATGCCGTTAA TTCTGGTCTT TTTTAAATTG
            //
            Primer: Amp_R
            Sequence file: RDB08530_A4L1d.seq check
            >08530_08530_A4L1_Amp_R_D09_12.ab1
                1 TACGGGGGGG GGCAACTCTT TTTATGACCG CTTTGGAAAA CAAAGACTGT ATTTCCCCGC
               61 GAAATTAATG TTTATTCAAT AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG
              121 AAATGAGGCG GGAAGAGGCG GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG
              181 CCTCCAAGGC GCCAGTCCTG TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC
              241 CAGAGTAAGC GGAGGCCAGG GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT
              301 GTCATAGTAA TGATTGGGTG GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT
              361 CTTGCAAAAC CACACTGACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA
              421 AAACCACACT GCCCTCTGGA GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC
              481 GGCCAGCTGC GGTCAGTTAG TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG
              541 AGTCGGGAGG AAAATTACTG TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT
              601 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
              661 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
              721 TTATCATGTC TGGATCTGAT ATCATCGTCG ACATTGATTA TTGACTAGCT AGTTATTAAT
              781 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
              841 TTACGGTAAA TGGCCCGCCC TGGCTGACCG CCCAACGACC CCCCGCCCAT TGACGTCAAT
              901 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGGACTTT TCCATTGACG TCAATGGGTG
              961 GAGTATTTTA CGGTAAACTG CCCACTTTGG CAGTACCATC AAAGTGTATC ATATGCCAAA
             1021 GTACCGCCCC CCTATTGGAC GTCAATGACG GTAAATGGGC CCGCCCTGGC ATTATGCCCA
             1081 GTACATGGAA CCTTAATGGG GACTTTCCTT ACTTGGCCAG TTACAATCTT AGCTTATTAG
             1141 TCATTCGCTA TTAACCATGG AATGGGCTCG AGGTGAAGGC CTCCCACGCG TTTCCTGTGC
            1201 CTTTACACTT CTTTCCCCCC CAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl