Resource data sheet
Prev.

pBRPyCAG-EGFP-IH

Expression clone of GFP under the control of CAG promoter, hygromycin resistance

Catalog number RDB08529
Resource name pBRPyCAG-EGFP-IH
Alternative name stock no 10683
Clone info. Expression clone of GFP under the control of CAG promoter, hygromycin resistance.
Vector backbone pBRPyCAG (Plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08529 pBRPyCAG-EGFP-IH DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08529z.seq from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13983_A6C8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB13983_A6C8a.seq
>08529_13983_A6C8_9_pAxCA_F1_H10_22.ab1
    1 AAGACGGAAA CGGGTTCATT CTACAATGTT TATGGCCTTC TTCTTTTTTC CTACAGCTCC
   61 TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGCCAC
  121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  481 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
  601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
  661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
  721 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
  781 CCTGCTGGAG TTCGTGACCG CCCGCCGGGG ATCACTCTCG GGCATGGGAC GAGCTGTACA
  841 AGTAAGCGGG CCGCTCGATA AGCTTGATAT CGAATTTCCG CCCCCCCCCC CCCTCTTCCC
901 T
//
Primer: IRES-F RDB13983_A6C8b.seq
>08529_13983_A6C8_9_IRES-F_A11_02.ab1
    1 ACAAGCCCTT TAATGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA AAAAGCCTGA
  121 ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT
  181 GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG
  241 ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTCATCG
  301 GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA
  361 GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA
  421 AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC
  481 CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC
  541 TACGTGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT
  601 GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC
  661 CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA AACAATGTCC
  721 TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG TTCGGGGATT
  781 CCCAATACGA GGTCGCCAAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TAATGGAGCA
  841 GCAGACGCGC TACTTTCGAG CCGGAGCATC CGGAGCTTGC AGGAATCGCC GCGGCTCCGG
  901 GGCGTAATAT GCTCCGCATT GGGTCTTGAA CCAACTCTAT CAAGAAGCTT TGATTGAACG
  961 GGCAAATTTC GATGAATGCA AGCTTTGGGC GCAAGGGTCC GAATGCGTAC GCAAATCGGT
 1021 CGGAATCGAA AGTCAGGAAC TGGTCGGGCG TACACAATTC CGCCTGCAAA GCCGACCGGT
1081 CTGAACGGAT GGCTGGTTAA AAAGTACCTC GCCCGAATAG TGAAATCCGG AAGC
//
Primer: Amp_R RDB13983_A6C8c.seq
>08529_13983_A6HC8_Amp_R_E09_15_ABI24.ab1
    1 CTCATTTTCA TGACGCTTTG GAAACAAAGA CTGTATTTCC TGGAAATTAA TGTTTATTCA
   61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG
  121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC
  181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA
  241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG
  301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA
  361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG
  421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT
  481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC
  541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC
  601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG
  721 ATATCATCGT CGACATTGAT TATTGACTAG CTAGTTATTA ATAGTAATCA ATTACGGGGT
  781 CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC
  841 CTGGCTGACC GCCCAACGAC CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
  901 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
  961 CACTTGGCAG TACATCAAGT GTATCATATG CCCAAGTACG CCCCCCCTTA TGGACGTCAT
 1021 GGACGGTAAA TGGACCGACT TGCAATATGG CCCAGTACAT GACCGTATGG CAACGTTTCG
1081 TTACTTGCCC AGGATAC
//

Please visit Sequencing and PCR primers for primer information.


References

References


2019.03.15

GNP_filter3_RDBDEP_html_190307.pl