Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBRPyCAG-EGFP-IH (#RDB08529)

Expression clone of GFP under the control of CAG promoter, hygromycin resistance

Alternative name stock no 10683
Clone info. Expression clone of GFP under the control of CAG promoter, hygromycin resistance.
Vector backbone pBRPyCAG (Plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Growth conditions 37 C, LB + Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB08529z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する()。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08529 pBRPyCAG-EGFP-IH DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBRPyCAG-EGFP-IH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08529).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13983_A6C8p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: EGFP 5'
Sequence file: RDB13983_A6C8e.seq check
>08529_13983_A6C8_9_pAxCA_F1_H10_22.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCTTC TTCNNTTTTN NNNNNNCTCC
   61 TGGGCNNCGT GCTGGTTATT GTGCTGTCTC NTCATTTTGG NNNANAATTC CTCGAGCCAC
  121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  481 GGTGAACCNN NNNNNNNTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACN ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
  601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
  661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGANAACCA
  721 CTACCTGAGC ACCCNGTCCG CCCTGAGCAA AGANCCCAAC GAGAAGCGCG NTCACATGGT
  781 CCTGCTGGAN TTCGTGACCG CCNNCNNGGG ATCACTCTCN GGCATGGNAC GANCTGTACA
  841 AGTAANCNGG NCGCTCNATA AGCTTGNTAT NNNANNTCNG CCCCCCCCCC CCNTCNNCCN
901 N
//
Primer: IRES-R2 (Pr0247)
Region: EGFP 3'
Sequence file: RDB13983_A6C8f.seq check
>08529_13983_B2EvF_IRES-R2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGGNNGGNN
   61 NNGGGGGGGG GGCGGAATTC GATATCNNGC TTATCGAGCG GCCGCTTACT TGTACAGCTC
  121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
  181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
  241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
  301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
  361 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAANAANA TGGTGCGCTC
  541 CTGGACGTAG CCTTCGGGCN TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  601 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCANC TTGCCGTANG TGGCATCGCC
  721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACN NCNCCGTCCA NCTCGACCAG
  781 NATGGGCACC ACCCCGGTGA ANAGCTCCTC GCCCTTGCTC ACCNNGGTGG CTCGAGGANN
  841 TCTTTGNCNA AATGATGAGA CANNNNNNTN ANCCAGCACG TTGCCCCNNG ANCTGTNNGA
901 AAANNANNAA NGNNNTGANC ANNNNNNNNN N
//
Primer: IRES-F (Pr0036)
Region: IRES,HygR
Sequence file: RDB13983_A6C8g.seq check
>08529_13983_A6C8_9_IRES-F_A11_02.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCGNNGNN NNNAANCGTC TAGGCCCCCC GNACCNCGGG
   61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA AAAAGCCTGA
  121 ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT
  181 GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG
  241 ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTCATCG
  301 GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA
  361 GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA
  421 AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC
  481 CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC
  541 TACGTGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT
  601 GATGGACGAC ACCGTCAGTG CGTCCGTCNC GCANGCTCTC GATGAGCTGA TGCTTTGGGC
  661 CNAGGACTGC CCCGAAGTCC GGCACCTCGT GCNCGCGGAT TTCGGCTNCN ANCNNTGTCC
  721 TGANNGACNA TGGCCGCNTA ACAGCGGTCA TTGACTGGAN CNANGCGANG TNCNGGGNNT
  781 NCCAATACNA GGTCGCCNAN NNTCNTCNTN NNNANGNNNN GNNNGGCNNG NNNNNNNNCA
  841 NCANANNCNC TNNNTNNNAG NNGNNNNNTN NNGNNCTTNN NNNNNNCNCN NNNGNNNCNN
901 GNNNNNNNNN GNNNNNNNNN N
//
Primer: Amp_R (Pr0008)
Region: polyoma ori,SV40 pA,HCMVIEE
Sequence file: RDB13983_A6C8h.seq check
>08529_13983_B2EvF_Amp_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNAAGNN TGTATTTCCT GGAAATTAAT
   61 GTTTATTCAA TAAACTGTGT ATTCAGCTAT ATTCACATAG TGGTGAGGCT GAAATGAGGC
  121 GGGAAGAGGC GGTTGGGGCT TAATTATATC AATTTGGGTG GCCCCACAGC GCCTCCAAGG
  181 CGCCAGTCCT GTTTTGACAA GTTGCCTCTG GAAGCCTCTC TTCTTTTTCT CCAGAGTAAG
  241 CGGAGGCCAG GGGCCCCCGG CCTCTGCTTA ATACTAAAAA AAACAGCTGT TGTCATAGTA
  301 ATGATTGGGT GGAAACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC TCTTGCAAAA
  361 CCACACTGAC ATTCCAGGCC TGGGTGGAGA GGCTTTTTGC TTCCTCTTGC AAAACCACAC
  421 TGCCCTCTGG AGGGCAGTTG CCTAGCAACT AATTAAAAGA GGATGTCGCA CGGCCAGCTG
  481 CGGTCAGTTA GTCACTTCCT GCTTAACTGA CTTGACATTT TCTATTTTAA GAGTCGGGAG
  541 GAAAATTACT GTGTTGGAGG CCCTCCGCCA TCTTCTGAAG CTGAATCGAA TTAACTTGTT
  601 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC
  661 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT
  721 CTGGATCTGA TATCATCGTC GACATTGATT ATTGACTAGC TAGTTATTAA TAGTAATCAA
  781 TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA
  841 ATGGCCCGCC TGGCTGANCG CCCAACGACC CCCGCCNATT GACGTCAATA ATGACGTATG
  901 TTCCCATANT AACGCNNATA GGGANTTTCC ATTGNNGTNA NTGGGTGGAN TATTTNNGGN
  961 NAACTGCCCN NTTNGNAGTA CATCANGTGT ATCATANNNC NNNGTANNNC CCCNNNNNNN
1021 NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles