Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-EGFP-IH

Expression clone of GFP under the control of CAG promoter, hygromycin resistance

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する()。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13983_A6C8p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: EGFP 5' Sequence file: RDB13983_A6C8e.seq
>08529_13983_A6C8_9_pAxCA_F1_H10_22.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCTTC TTCNNTTTTN NNNNNNCTCC    61 TGGGCNNCGT GCTGGTTATT GTGCTGTCTC NTCATTTTGG NNNANAATTC CTCGAGCCAC   121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA   181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA   241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC   301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA   361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT   421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT   481 GGTGAACCNN NNNNNNNTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA   541 CAAGCTGGAG TACAACTACN ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA   601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC   661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGANAACCA   721 CTACCTGAGC ACCCNGTCCG CCCTGAGCAA AGANCCCAAC GAGAAGCGCG NTCACATGGT   781 CCTGCTGGAN TTCGTGACCG CCNNCNNGGG ATCACTCTCN GGCATGGNAC GANCTGTACA   841 AGTAANCNGG NCGCTCNATA AGCTTGNTAT NNNANNTCNG CCCCCCCCCC CCNTCNNCCN 901 N //
Primer: IRES-R2 (Pr0247) Region: EGFP 3' Sequence file: RDB13983_A6C8f.seq
>08529_13983_B2EvF_IRES-R2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGGNNGGNN    61 NNGGGGGGGG GGCGGAATTC GATATCNNGC TTATCGAGCG GCCGCTTACT TGTACAGCTC   121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG   181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG   241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT   301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC   361 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT   421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC   481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAANAANA TGGTGCGCTC   541 CTGGACGTAG CCTTCGGGCN TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG   601 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC   661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCANC TTGCCGTANG TGGCATCGCC   721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACN NCNCCGTCCA NCTCGACCAG   781 NATGGGCACC ACCCCGGTGA ANAGCTCCTC GCCCTTGCTC ACCNNGGTGG CTCGAGGANN   841 TCTTTGNCNA AATGATGAGA CANNNNNNTN ANCCAGCACG TTGCCCCNNG ANCTGTNNGA 901 AAANNANNAA NGNNNTGANC ANNNNNNNNN N //
Primer: IRES-F (Pr0036) Region: IRES,HygR Sequence file: RDB13983_A6C8g.seq
>08529_13983_A6C8_9_IRES-F_A11_02.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCGNNGNN NNNAANCGTC TAGGCCCCCC GNACCNCGGG    61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA AAAAGCCTGA   121 ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT   181 GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG   241 ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTCATCG   301 GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA   361 GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA   421 AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC   481 CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC   541 TACGTGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT   601 GATGGACGAC ACCGTCAGTG CGTCCGTCNC GCANGCTCTC GATGAGCTGA TGCTTTGGGC   661 CNAGGACTGC CCCGAAGTCC GGCACCTCGT GCNCGCGGAT TTCGGCTNCN ANCNNTGTCC   721 TGANNGACNA TGGCCGCNTA ACAGCGGTCA TTGACTGGAN CNANGCGANG TNCNGGGNNT   781 NCCAATACNA GGTCGCCNAN NNTCNTCNTN NNNANGNNNN GNNNGGCNNG NNNNNNNNCA   841 NCANANNCNC TNNNTNNNAG NNGNNNNNTN NNGNNCTTNN NNNNNNCNCN NNNGNNNCNN 901 GNNNNNNNNN GNNNNNNNNN N //
Primer: Amp_R (Pr0008) Region: polyoma ori,SV40 pA,HCMVIEE Sequence file: RDB13983_A6C8h.seq
>08529_13983_B2EvF_Amp_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNAAGNN TGTATTTCCT GGAAATTAAT    61 GTTTATTCAA TAAACTGTGT ATTCAGCTAT ATTCACATAG TGGTGAGGCT GAAATGAGGC   121 GGGAAGAGGC GGTTGGGGCT TAATTATATC AATTTGGGTG GCCCCACAGC GCCTCCAAGG   181 CGCCAGTCCT GTTTTGACAA GTTGCCTCTG GAAGCCTCTC TTCTTTTTCT CCAGAGTAAG   241 CGGAGGCCAG GGGCCCCCGG CCTCTGCTTA ATACTAAAAA AAACAGCTGT TGTCATAGTA   301 ATGATTGGGT GGAAACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC TCTTGCAAAA   361 CCACACTGAC ATTCCAGGCC TGGGTGGAGA GGCTTTTTGC TTCCTCTTGC AAAACCACAC   421 TGCCCTCTGG AGGGCAGTTG CCTAGCAACT AATTAAAAGA GGATGTCGCA CGGCCAGCTG   481 CGGTCAGTTA GTCACTTCCT GCTTAACTGA CTTGACATTT TCTATTTTAA GAGTCGGGAG   541 GAAAATTACT GTGTTGGAGG CCCTCCGCCA TCTTCTGAAG CTGAATCGAA TTAACTTGTT   601 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC   661 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT   721 CTGGATCTGA TATCATCGTC GACATTGATT ATTGACTAGC TAGTTATTAA TAGTAATCAA   781 TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA   841 ATGGCCCGCC TGGCTGANCG CCCAACGACC CCCGCCNATT GACGTCAATA ATGACGTATG   901 TTCCCATANT AACGCNNATA GGGANTTTCC ATTGNNGTNA NTGGGTGGAN TATTTNNGGN   961 NAACTGCCCN NTTNGNAGTA CATCANGTGT ATCATANNNC NNNGTANNNC CCCNNNNNNN 1021 NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl