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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBRPyCAG-EGFP-IH

Expression clone of GFP under the control of CAG promoter, hygromycin resistance

Catalog number RDB08529
Resource name pBRPyCAG-EGFP-IH
Alternative name stock no 10683
Clone info. Expression clone of GFP under the control of CAG promoter, hygromycin resistance.
Vector backbone pBRPyCAG (Plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence RDB08529z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する()。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08529 pBRPyCAG-EGFP-IH DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13983_A6C8p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Region: EGFP 5'
            Sequence file: RDB13983_A6C8e.seq check
            >08529_13983_A6C8_9_pAxCA_F1_H10_22.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCTTC TTCNNTTTTN NNNNNNCTCC
               61 TGGGCNNCGT GCTGGTTATT GTGCTGTCTC NTCATTTTGG NNNANAATTC CTCGAGCCAC
              121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
              181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
              241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
              301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
              361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
              421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
              481 GGTGAACCNN NNNNNNNTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
              541 CAAGCTGGAG TACAACTACN ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
              601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
              661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGANAACCA
              721 CTACCTGAGC ACCCNGTCCG CCCTGAGCAA AGANCCCAAC GAGAAGCGCG NTCACATGGT
              781 CCTGCTGGAN TTCGTGACCG CCNNCNNGGG ATCACTCTCN GGCATGGNAC GANCTGTACA
              841 AGTAANCNGG NCGCTCNATA AGCTTGNTAT NNNANNTCNG CCCCCCCCCC CCNTCNNCCN
            901 N
            //
            Primer: IRES-R2
            Region: EGFP 3'
            Sequence file: RDB13983_A6C8f.seq check
            >08529_13983_B2EvF_IRES-R2_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGGNNGGNN
               61 NNGGGGGGGG GGCGGAATTC GATATCNNGC TTATCGAGCG GCCGCTTACT TGTACAGCTC
              121 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
              181 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
              241 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
              301 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
              361 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
              421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
              481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAANAANA TGGTGCGCTC
              541 CTGGACGTAG CCTTCGGGCN TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
              601 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
              661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCANC TTGCCGTANG TGGCATCGCC
              721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACN NCNCCGTCCA NCTCGACCAG
              781 NATGGGCACC ACCCCGGTGA ANAGCTCCTC GCCCTTGCTC ACCNNGGTGG CTCGAGGANN
              841 TCTTTGNCNA AATGATGAGA CANNNNNNTN ANCCAGCACG TTGCCCCNNG ANCTGTNNGA
            901 AAANNANNAA NGNNNTGANC ANNNNNNNNN N
            //
            Primer: IRES-F
            Region: IRES,HygR
            Sequence file: RDB13983_A6C8g.seq check
            >08529_13983_A6C8_9_IRES-F_A11_02.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCGNNGNN NNNAANCGTC TAGGCCCCCC GNACCNCGGG
               61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA AAAAGCCTGA
              121 ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT
              181 GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG
              241 ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTCATCG
              301 GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA
              361 GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA
              421 AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC
              481 CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC
              541 TACGTGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT
              601 GATGGACGAC ACCGTCAGTG CGTCCGTCNC GCANGCTCTC GATGAGCTGA TGCTTTGGGC
              661 CNAGGACTGC CCCGAAGTCC GGCACCTCGT GCNCGCGGAT TTCGGCTNCN ANCNNTGTCC
              721 TGANNGACNA TGGCCGCNTA ACAGCGGTCA TTGACTGGAN CNANGCGANG TNCNGGGNNT
              781 NCCAATACNA GGTCGCCNAN NNTCNTCNTN NNNANGNNNN GNNNGGCNNG NNNNNNNNCA
              841 NCANANNCNC TNNNTNNNAG NNGNNNNNTN NNGNNCTTNN NNNNNNCNCN NNNGNNNCNN
            901 GNNNNNNNNN GNNNNNNNNN N
            //
            Primer: Amp_R
            Region: polyoma ori,SV40 pA,HCMVIEE
            Sequence file: RDB13983_A6C8h.seq check
            >08529_13983_B2EvF_Amp_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNAAGNN TGTATTTCCT GGAAATTAAT
               61 GTTTATTCAA TAAACTGTGT ATTCAGCTAT ATTCACATAG TGGTGAGGCT GAAATGAGGC
              121 GGGAAGAGGC GGTTGGGGCT TAATTATATC AATTTGGGTG GCCCCACAGC GCCTCCAAGG
              181 CGCCAGTCCT GTTTTGACAA GTTGCCTCTG GAAGCCTCTC TTCTTTTTCT CCAGAGTAAG
              241 CGGAGGCCAG GGGCCCCCGG CCTCTGCTTA ATACTAAAAA AAACAGCTGT TGTCATAGTA
              301 ATGATTGGGT GGAAACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC TCTTGCAAAA
              361 CCACACTGAC ATTCCAGGCC TGGGTGGAGA GGCTTTTTGC TTCCTCTTGC AAAACCACAC
              421 TGCCCTCTGG AGGGCAGTTG CCTAGCAACT AATTAAAAGA GGATGTCGCA CGGCCAGCTG
              481 CGGTCAGTTA GTCACTTCCT GCTTAACTGA CTTGACATTT TCTATTTTAA GAGTCGGGAG
              541 GAAAATTACT GTGTTGGAGG CCCTCCGCCA TCTTCTGAAG CTGAATCGAA TTAACTTGTT
              601 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC
              661 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT
              721 CTGGATCTGA TATCATCGTC GACATTGATT ATTGACTAGC TAGTTATTAA TAGTAATCAA
              781 TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA
              841 ATGGCCCGCC TGGCTGANCG CCCAACGACC CCCGCCNATT GACGTCAATA ATGACGTATG
              901 TTCCCATANT AACGCNNATA GGGANTTTCC ATTGNNGTNA NTGGGTGGAN TATTTNNGGN
              961 NAACTGCCCN NTTNGNAGTA CATCANGTGT ATCATANNNC NNNGTANNNC CCCNNNNNNN
            1021 NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.10.12

            GNP_filter3_RDBDEP_html_221010.pl