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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-EGFP-IB

Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.

Catalog number RDB08528
Resource name pPyCAG-EGFP-IB
Alternative name stock no 10681
Clone info. Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08528 pPyCAG-EGFP-IB DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB08528z.seq
            Remarks, protocol and/or map (gif) RDB08528a.gif

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08528_A6Hvp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxCA_F1
            Sequence file: RDB08528_A6Hva.seq check
            >08528_08528_A6Hv_pAxCA_F1_A08_02_ABI24.ab1
                1 GTTATCACTG CTCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
               61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGCCACCATG GTGAGCAAGG
              121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
              181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
              241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
              301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
              361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
              421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
              481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
              541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
              601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
              661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
              721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
              781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGCG GCCGCTCGAT
              841 AAGCTTGATA TCGAATTCGC CCCCCCCCCC CCCTCTCCCT CCCCCCCCCC CCTAACGTTA
              901 CTGGCCCGAA GCCCGCTTGG AATAGGCCCG GTGTGCGTTT GTCTATATGT ATTTTCCACA
              961 TATGCCGTCT TTTGGCCAAT GTGAAGGGCC AGAAACTTGA TCTGTCCTTC CTGGACGAGC
             1021 CATCCTAAGG TTCTACCCTC CTCGCCAGAT GCAGTCTGTG AATGTCATGA AGGAAGGCAG
            //
            Primer: IRES-F
            Sequence file: RDB08528_A6Hvb.seq check
            >08528_08528_A6Hv_IRES-F_B08_05_ABI24.ab1
                1 TCATCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
               61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGCCTTT GTCTCAAGAA
              121 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC
              181 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA
              241 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
              301 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC
              361 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG
              421 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT
              481 GTGTGGGAGG GTTAACTAGA TAAGTAATGA TCATAATCAG CCATATCACA TCTGTAGAGG
              541 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG
              601 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
              661 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGG TTTGTCCAAA
              721 CTCATCAATG TATCTTATCA TGTCTGGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG
              781 GGGAGAGGCG GTTTGCGTAT TGGGGCGCTC TTCGCTCTCG CTCACTGACT CGCTGCGCTC
              841 GTCGTCGCTG CGGCGAGCGG TATCAGCTCA CTCAAGCGTA TACGGTATCA CAGAATTCAA
              901 GGGATACCGC AAGAAAGAAC ATTGTGAGCC AAATGCAGCC AAAGTCAGAA TCGTAAAAGC
              961 CCGATGCCTG GCGCTTCCAT AGCTCGCCCC TGACAGCATT CCATTCGAGC CTAGTCCAAG
            1021 GTGCAATG
            //
            Primer: Amp_R
            Sequence file: RDB08528_A6Hvc.seq check
            >08528_08528_A6Hv_Amp_R_C08_08_ABI24.ab1
                1 CTCTTTTATG ACGCTTTGGA AACAAAGACT GTATTTCCTG GAAATTAATG TTTATTCAAT
               61 AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG AAATGAGGCG GGAAGAGGCG
              121 GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG CCTCCAAGGC GCCAGTCCTG
              181 TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC CAGAGTAAGC GGAGGCCAGG
              241 GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT GTCATAGTAA TGATTGGGTG
              301 GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGACA
              361 TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT GCCCTCTGGA
              421 GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC GGCCAGCTGC GGTCAGTTAG
              481 TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG AGTCGGGAGG AAAATTACTG
              541 TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT TAACTTGTTT ATTGCAGCTT
              601 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
              661 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGAT
              721 ATCATCGTCG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
              781 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
              841 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
              901 CAATAGGACT TTCCATTGAC GTCATGGGGT GGAGTATTTA CCGTAAACTG CCCACTTGCA
              961 GTACATCAAG TGTATCATAT GCTAGTTACG CCCCTAATTG ACGTTCATTG ACCGTTAATG
            1021 GACTGACCTT GGCATAATGG CCGAGTAATG ACCGTATGGA ACCTTACGTA CTTTGGCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl