Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-EGFP-IB

Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Sequence RDB08528z.seq
Electronic file	Map RDB08528a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08528_A6Hvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08528_A6Hva.seq
>08528_08528_A6Hv_pAxCA_F1_A08_02_ABI24.ab1     1 GTTATCACTG CTCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG    61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGCCACCATG GTGAGCAAGG   121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC   241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC   301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT   361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG   421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG   481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA   541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA   601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC   661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC   721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG   781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGCG GCCGCTCGAT   841 AAGCTTGATA TCGAATTCGC CCCCCCCCCC CCCTCTCCCT CCCCCCCCCC CCTAACGTTA   901 CTGGCCCGAA GCCCGCTTGG AATAGGCCCG GTGTGCGTTT GTCTATATGT ATTTTCCACA   961 TATGCCGTCT TTTGGCCAAT GTGAAGGGCC AGAAACTTGA TCTGTCCTTC CTGGACGAGC  1021 CATCCTAAGG TTCTACCCTC CTCGCCAGAT GCAGTCTGTG AATGTCATGA AGGAAGGCAG //
Primer: IRES-F Sequence file: RDB08528_A6Hvb.seq
>08528_08528_A6Hv_IRES-F_B08_05_ABI24.ab1     1 TCATCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG    61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGCCTTT GTCTCAAGAA   121 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC   181 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA   241 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG   301 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC   361 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG   421 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT   481 GTGTGGGAGG GTTAACTAGA TAAGTAATGA TCATAATCAG CCATATCACA TCTGTAGAGG   541 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG   601 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA   661 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGG TTTGTCCAAA   721 CTCATCAATG TATCTTATCA TGTCTGGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG   781 GGGAGAGGCG GTTTGCGTAT TGGGGCGCTC TTCGCTCTCG CTCACTGACT CGCTGCGCTC   841 GTCGTCGCTG CGGCGAGCGG TATCAGCTCA CTCAAGCGTA TACGGTATCA CAGAATTCAA   901 GGGATACCGC AAGAAAGAAC ATTGTGAGCC AAATGCAGCC AAAGTCAGAA TCGTAAAAGC   961 CCGATGCCTG GCGCTTCCAT AGCTCGCCCC TGACAGCATT CCATTCGAGC CTAGTCCAAG 1021 GTGCAATG //
Primer: Amp_R Sequence file: RDB08528_A6Hvc.seq
>08528_08528_A6Hv_Amp_R_C08_08_ABI24.ab1     1 CTCTTTTATG ACGCTTTGGA AACAAAGACT GTATTTCCTG GAAATTAATG TTTATTCAAT    61 AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG AAATGAGGCG GGAAGAGGCG   121 GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG CCTCCAAGGC GCCAGTCCTG   181 TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC CAGAGTAAGC GGAGGCCAGG   241 GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT GTCATAGTAA TGATTGGGTG   301 GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGACA   361 TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT GCCCTCTGGA   421 GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC GGCCAGCTGC GGTCAGTTAG   481 TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG AGTCGGGAGG AAAATTACTG   541 TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT TAACTTGTTT ATTGCAGCTT   601 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC   661 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGAT   721 ATCATCGTCG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG   781 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT   841 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC   901 CAATAGGACT TTCCATTGAC GTCATGGGGT GGAGTATTTA CCGTAAACTG CCCACTTGCA   961 GTACATCAAG TGTATCATAT GCTAGTTACG CCCCTAATTG ACGTTCATTG ACCGTTAATG 1021 GACTGACCTT GGCATAATGG CCGAGTAATG ACCGTATGGA ACCTTACGTA CTTTGGCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl