Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.
| Catalog number | RDB08528 |
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| Resource name | pPyCAG-EGFP-IB |
| Alternative name | stock no 10681 |
| Clone info. | Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance. |
| Vector backbone | pPyCAG (Plasmid) |
| Size of vector backbone | 5.6 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Aequorea victoria GFP cDNA |
| Depositor | Niwa, Hitoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08528 | pPyCAG-EGFP-IB | DNA solution |
Electronic file
| Electronic file | Sequence RDB08528z.seq from Depositor |
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| Electronic file | Map RDB08528a.gif from Depositor
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Featured content
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08528_A6Hvp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxCA_F1 | RDB08528_A6Hva.seq |
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>08528_08528_A6Hv_pAxCA_F1_A08_02_ABI24.ab1 1 GTTATCACTG CTCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG 61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGCCACCATG GTGAGCAAGG 121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC 301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA 601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC 661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC 721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGCG GCCGCTCGAT 841 AAGCTTGATA TCGAATTCGC CCCCCCCCCC CCCTCTCCCT CCCCCCCCCC CCTAACGTTA 901 CTGGCCCGAA GCCCGCTTGG AATAGGCCCG GTGTGCGTTT GTCTATATGT ATTTTCCACA 961 TATGCCGTCT TTTGGCCAAT GTGAAGGGCC AGAAACTTGA TCTGTCCTTC CTGGACGAGC 1021 CATCCTAAGG TTCTACCCTC CTCGCCAGAT GCAGTCTGTG AATGTCATGA AGGAAGGCAG // |
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| Primer: IRES-F | RDB08528_A6Hvb.seq |
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>08528_08528_A6Hv_IRES-F_B08_05_ABI24.ab1 1 TCATCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGCCTTT GTCTCAAGAA 121 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC 181 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA 241 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG 301 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC 361 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG 421 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT 481 GTGTGGGAGG GTTAACTAGA TAAGTAATGA TCATAATCAG CCATATCACA TCTGTAGAGG 541 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG 601 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 661 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGG TTTGTCCAAA 721 CTCATCAATG TATCTTATCA TGTCTGGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG 781 GGGAGAGGCG GTTTGCGTAT TGGGGCGCTC TTCGCTCTCG CTCACTGACT CGCTGCGCTC 841 GTCGTCGCTG CGGCGAGCGG TATCAGCTCA CTCAAGCGTA TACGGTATCA CAGAATTCAA 901 GGGATACCGC AAGAAAGAAC ATTGTGAGCC AAATGCAGCC AAAGTCAGAA TCGTAAAAGC 961 CCGATGCCTG GCGCTTCCAT AGCTCGCCCC TGACAGCATT CCATTCGAGC CTAGTCCAAG 1021 GTGCAATG // |
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| Primer: Amp_R | RDB08528_A6Hvc.seq |
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>08528_08528_A6Hv_Amp_R_C08_08_ABI24.ab1 1 CTCTTTTATG ACGCTTTGGA AACAAAGACT GTATTTCCTG GAAATTAATG TTTATTCAAT 61 AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG AAATGAGGCG GGAAGAGGCG 121 GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG CCTCCAAGGC GCCAGTCCTG 181 TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC CAGAGTAAGC GGAGGCCAGG 241 GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT GTCATAGTAA TGATTGGGTG 301 GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGACA 361 TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT GCCCTCTGGA 421 GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC GGCCAGCTGC GGTCAGTTAG 481 TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG AGTCGGGAGG AAAATTACTG 541 TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT TAACTTGTTT ATTGCAGCTT 601 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 661 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGAT 721 ATCATCGTCG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG 781 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT 841 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC 901 CAATAGGACT TTCCATTGAC GTCATGGGGT GGAGTATTTA CCGTAAACTG CCCACTTGCA 961 GTACATCAAG TGTATCATAT GCTAGTTACG CCCCTAATTG ACGTTCATTG ACCGTTAATG 1021 GACTGACCTT GGCATAATGG CCGAGTAATG ACCGTATGGA ACCTTACGTA CTTTGGCA // |
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Sequencing and PCR primers for primer information.
References
2019.02.18
GNP_filter3_RDBDEP_html_190131.pl