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pPyCAG-EGFP-IB

Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.

Catalog number RDB08528
Resource name pPyCAG-EGFP-IB
Alternative name stock no 10681
Clone info. Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested().
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Schedule A (GFP-A) [Link]
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RDB08528 pPyCAG-EGFP-IB DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08528z.seq from Depositor
Electronic file Map RDB08528a.gif from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08528_A6Hvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB08528_A6Hva.seq
>08528_08528_A6Hv_pAxCA_F1_A08_02_ABI24.ab1
    1 GTTATCACTG CTCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGCCACCATG GTGAGCAAGG
  121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
  361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
  421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
  481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
  541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
  601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
  661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
  721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
  781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGCG GCCGCTCGAT
  841 AAGCTTGATA TCGAATTCGC CCCCCCCCCC CCCTCTCCCT CCCCCCCCCC CCTAACGTTA
  901 CTGGCCCGAA GCCCGCTTGG AATAGGCCCG GTGTGCGTTT GTCTATATGT ATTTTCCACA
  961 TATGCCGTCT TTTGGCCAAT GTGAAGGGCC AGAAACTTGA TCTGTCCTTC CTGGACGAGC
 1021 CATCCTAAGG TTCTACCCTC CTCGCCAGAT GCAGTCTGTG AATGTCATGA AGGAAGGCAG
//
Primer: IRES-F RDB08528_A6Hvb.seq
>08528_08528_A6Hv_IRES-F_B08_05_ABI24.ab1
    1 TCATCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGCCTTT GTCTCAAGAA
  121 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC
  181 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA
  241 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
  301 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC
  361 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG
  421 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT
  481 GTGTGGGAGG GTTAACTAGA TAAGTAATGA TCATAATCAG CCATATCACA TCTGTAGAGG
  541 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG
  601 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
  661 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGG TTTGTCCAAA
  721 CTCATCAATG TATCTTATCA TGTCTGGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG
  781 GGGAGAGGCG GTTTGCGTAT TGGGGCGCTC TTCGCTCTCG CTCACTGACT CGCTGCGCTC
  841 GTCGTCGCTG CGGCGAGCGG TATCAGCTCA CTCAAGCGTA TACGGTATCA CAGAATTCAA
  901 GGGATACCGC AAGAAAGAAC ATTGTGAGCC AAATGCAGCC AAAGTCAGAA TCGTAAAAGC
  961 CCGATGCCTG GCGCTTCCAT AGCTCGCCCC TGACAGCATT CCATTCGAGC CTAGTCCAAG
1021 GTGCAATG
//
Primer: Amp_R RDB08528_A6Hvc.seq
>08528_08528_A6Hv_Amp_R_C08_08_ABI24.ab1
    1 CTCTTTTATG ACGCTTTGGA AACAAAGACT GTATTTCCTG GAAATTAATG TTTATTCAAT
   61 AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG AAATGAGGCG GGAAGAGGCG
  121 GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG CCTCCAAGGC GCCAGTCCTG
  181 TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC CAGAGTAAGC GGAGGCCAGG
  241 GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT GTCATAGTAA TGATTGGGTG
  301 GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGACA
  361 TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT GCCCTCTGGA
  421 GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC GGCCAGCTGC GGTCAGTTAG
  481 TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG AGTCGGGAGG AAAATTACTG
  541 TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT TAACTTGTTT ATTGCAGCTT
  601 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
  661 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGAT
  721 ATCATCGTCG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
  781 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
  841 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
  901 CAATAGGACT TTCCATTGAC GTCATGGGGT GGAGTATTTA CCGTAAACTG CCCACTTGCA
  961 GTACATCAAG TGTATCATAT GCTAGTTACG CCCCTAATTG ACGTTCATTG ACCGTTAATG
1021 GACTGACCTT GGCATAATGG CCGAGTAATG ACCGTATGGA ACCTTACGTA CTTTGGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2019.02.18

GNP_filter3_RDBDEP_html_190131.pl