RDB08528 - pPyCAG-EGFP-IB

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pPyCAG-EGFP-IB (#RDB08528)

Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.

Alternative name	stock no 10681
Clone info.	Expression clone of GFP under the control of CAG promoter, Blasticidin S resistance.
Vector backbone	pPyCAG (Plasmid)
Size of vector backbone	5.6 kb
Selectable markers	Amp^r
Growth conditions	37 C, LB + Amp
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Niwa, Hitoshi \|

Sequence	RDB08528z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08528	pPyCAG-EGFP-IB	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-EGFP-IB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08528).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08528_A6Hvp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB08528_A6Hva.seq
>08528_08528_A6Hv_pAxCA_F1_A08_02_ABI24.ab1 1 GTTATCACTG CTCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG 61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGCCACCATG GTGAGCAAGG 121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC 301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA 601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC 661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC 721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGCG GCCGCTCGAT 841 AAGCTTGATA TCGAATTCGC CCCCCCCCCC CCCTCTCCCT CCCCCCCCCC CCTAACGTTA 901 CTGGCCCGAA GCCCGCTTGG AATAGGCCCG GTGTGCGTTT GTCTATATGT ATTTTCCACA 961 TATGCCGTCT TTTGGCCAAT GTGAAGGGCC AGAAACTTGA TCTGTCCTTC CTGGACGAGC 1021 CATCCTAAGG TTCTACCCTC CTCGCCAGAT GCAGTCTGTG AATGTCATGA AGGAAGGCAG //
Primer: IRES-F Sequence file: RDB08528_A6Hvb.seq
>08528_08528_A6Hv_IRES-F_B08_05_ABI24.ab1 1 TCATCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGCCTTT GTCTCAAGAA 121 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC 181 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA 241 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG 301 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC 361 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG 421 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT 481 GTGTGGGAGG GTTAACTAGA TAAGTAATGA TCATAATCAG CCATATCACA TCTGTAGAGG 541 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG 601 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 661 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGG TTTGTCCAAA 721 CTCATCAATG TATCTTATCA TGTCTGGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG 781 GGGAGAGGCG GTTTGCGTAT TGGGGCGCTC TTCGCTCTCG CTCACTGACT CGCTGCGCTC 841 GTCGTCGCTG CGGCGAGCGG TATCAGCTCA CTCAAGCGTA TACGGTATCA CAGAATTCAA 901 GGGATACCGC AAGAAAGAAC ATTGTGAGCC AAATGCAGCC AAAGTCAGAA TCGTAAAAGC 961 CCGATGCCTG GCGCTTCCAT AGCTCGCCCC TGACAGCATT CCATTCGAGC CTAGTCCAAG 1021 GTGCAATG //
Primer: Amp_R Sequence file: RDB08528_A6Hvc.seq
>08528_08528_A6Hv_Amp_R_C08_08_ABI24.ab1 1 CTCTTTTATG ACGCTTTGGA AACAAAGACT GTATTTCCTG GAAATTAATG TTTATTCAAT 61 AAACTGTGTA TTCAGCTATA TTCACATAGT GGTGAGGCTG AAATGAGGCG GGAAGAGGCG 121 GTTGGGGCTT AATTATATCA ATTTGGGTGG CCCCACAGCG CCTCCAAGGC GCCAGTCCTG 181 TTTTGACAAG TTGCCTCTGG AAGCCTCTCT TCTTTTTCTC CAGAGTAAGC GGAGGCCAGG 241 GGCCCCCGGC CTCTGCTTAA TACTAAAAAA AACAGCTGTT GTCATAGTAA TGATTGGGTG 301 GAAACATTCC AGGCCTGGGT GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGACA 361 TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT GCCCTCTGGA 421 GGGCAGTTGC CTAGCAACTA ATTAAAAGAG GATGTCGCAC GGCCAGCTGC GGTCAGTTAG 481 TCACTTCCTG CTTAACTGAC TTGACATTTT CTATTTTAAG AGTCGGGAGG AAAATTACTG 541 TGTTGGAGGC CCTCCGCCAT CTTCTGAAGC TGAATCGAAT TAACTTGTTT ATTGCAGCTT 601 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 661 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTGAT 721 ATCATCGTCG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG 781 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT 841 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC 901 CAATAGGACT TTCCATTGAC GTCATGGGGT GGAGTATTTA CCGTAAACTG CCCACTTGCA 961 GTACATCAAG TGTATCATAT GCTAGTTACG CCCCTAATTG ACGTTCATTG ACCGTTAATG 1021 GACTGACCTT GGCATAATGG CCGAGTAATG ACCGTATGGA ACCTTACGTA CTTTGGCA //